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Citations for Cambridge Isotope Labs :
1 - 50 of 61 citations for Retinol Binding Protein RBP Multi format ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Ultra pure bovine serum albumin (Cell Signalling Technology 9998) was used as a protein source and iPSDM were incubated with media containing 13C-labeled glucose (Cambridge Isotope Laboratories CLM-1396-5) and the described treatments for 1h before continuing with downstream sample processing ...
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bioRxiv - Neuroscience 2023Quote: ... and heavy isotopes (4, 4, 5, 5-D4 L-lysine and 13C6 L-arginine, Cambridge Isotope Laboratories (CIL)) for the K4/R6 population ...
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bioRxiv - Biochemistry 2019Quote: ... 13C2-acetate (Cambridge Isotope Laboratories CNLM-440-5), 13C6-glucose (Cambridge Isotope Laboratories CLM-1396-1) ...
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bioRxiv - Molecular Biology 2024Quote: ... [15N]5-2-deoxyadenosine was obtained from Cambridge Isotope Laboratories ...
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bioRxiv - Cell Biology 2024Quote: ... or U-13C-glucose (Cambridge Isotope Laboratories: CLM-1396-5), which replaces the unlabeled glutamine or glucose in regular media at the same concentration ...
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bioRxiv - Cancer Biology 2023Quote: ... or 5 mM 13C1,2-glucose (CLM-504-PK, Cambridge Isotope Laboratories) and 10% dialyzed FBS (F0392 ...
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bioRxiv - Genetics 2021Quote: ... coli kit (MS-CRED-KIT) was purchased from Cambridge Isotope Laboratories ...
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bioRxiv - Biochemistry 2021Quote: ... 5 mL 15N Bioexpress Cell Growth Medium (Cambridge Isotope Laboratories, Tewksbury, MA, USA), 5 mL of Gibco MEM Vitamin Solution (100x) ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.05 U Phosphodiesterase I (Sigma # P3243)] and [15N]5-2’-deoxyadenosine ([15N]-dA) (Cambridge Isotope Laboratories # NLM-3895-25) as the internal standard to account for variations in sample handling and mass spectrometer response59 ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were incubated with either 13C6-glucose (CLM-1396-5, Cambridge Isotope Laboratories) or 15N2-glutamine (NLM-1328-0.25 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were blocked with 50mM ammonium chloride (Cambridge Isotope Laboratories, NLM-467-5), 0.01% bovine serum albumin (BSA ...
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bioRxiv - Biochemistry 2021Quote: ... protein was expressed in M9 minimal media with 15N-NH4Cl (Cambridge Isotope labs) as the sole nitrogen source instead of 2xYT media ...
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bioRxiv - Systems Biology 2020Quote: ... four plates of each species were treated with 2.5 mM L-proline (13C5, 99%; Cambridge Isotope Laboratories) and four with 2.5 mM AZC ...
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bioRxiv - Bioengineering 2021Quote: ... 5 μL of 100/3 mM DSS-D6 in deuterium oxide (Cambridge Isotope Laboratories) were added to 1.7 mm NMR tubes (Bruker BioSpin) ...
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bioRxiv - Biochemistry 2023Quote: ... but glucose was omitted and instead 5 mM 13C3-U-glycerol (Cambridge Isotope Laboratories) was supplied ...
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bioRxiv - Cell Biology 2020Quote: ... This DMEM contained 2 mM [5-13C 2-15N] labelled glutamine (Cambridge Isotope Laboratories, Inc.), 5.5 mM glucose ...
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bioRxiv - Microbiology 2023Quote: ... We spiked 13C and 15N labeled algal AAs or protein (Cambridge Isotope Laboratories, Inc., MA) into late-log phase ...
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bioRxiv - Bioengineering 2022Quote: ... 100 μl of 5 M isotope labelled D-Glucose (U-13C6, 99% from Cambridge Isotope Laboratories) prior to feeding ...
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bioRxiv - Neuroscience 2020Quote: Primary hippocampal neurons were prepared from E18-19 rat brains and grown on poly-D-lysine coated 15 cm plates at a density of 3.5 million cells/ 15 cm plate in DMEM media without l-arginine or l-lysine (Cambridge Isotope Laboratories ...
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bioRxiv - Microbiology 2020Quote: ... Each carbohydrate (about 5 mg) was dissolved in about 550 µl DH2O (Cambridge Isotope Laboratories, 99.8+% deuterated). Chemical shift referencing was accomplished relative to TMSP at 0.0 ppm ...
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bioRxiv - Microbiology 2023Quote: ... The dry MeOH extract (∼5 mg) was dissolved in 1 ml deuterated methanol (CD3OD; Cambridge Isotope Laboratories) for analysis by proton nuclear magnetic resonance (1H NMR ...
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bioRxiv - Microbiology 2024Quote: ... they were diluted 1:5 with an internal standard mixture (L-Adenine-8-13C (Cambridge Isotope Lab), L-Phenyl-d5-Alanine-2,3,3-d3 (Cambridge Isotope Lab) ...
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Conformational dynamics at microsecond timescale in the RNA-binding regions of dsRNA-binding domainsbioRxiv - Biophysics 2019Quote: ... the protein was prepared by growing the cells in the M9 media containing 15NH4Cl (Cambridge Isotope Laboratories) as a sole source of nitrogen.
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bioRxiv - Cancer Biology 2021Quote: ... 7.0 × 105 cells were plated onto 6 well plates for next day pulse-labeling with either 25 mM [1,2-13C] glucose (Cambridge Isotope Laboratories). Isotope labeling experiments were performed for 4 hours and extra plates were included for cell counts ...
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bioRxiv - Immunology 2022Quote: ... and re-cultured (2.5 × 106 cells/well in 24-well plates) for indicated times in medium containing 13C-labeled metabolites (Cambridge Isotope Laboratories) at the following concentrations ...
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bioRxiv - Cancer Biology 2021Quote: ... CAFs seeded were seeded in 6 well plates at 2×105 cells/well and incubated for 72 hours in growth media containing U13C-Glucose (Cambridge Isotope Laboratories).
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bioRxiv - Physiology 2024Quote: ... the cells were placed in 10 cm plates with media where the normal glucose was replaced with 13C6-L-glucose (Cambridge Isotope Laboratories). Cells were allowed to incubate in the 13C6-L-glucose prior to the in vitro ischemic-reperfusion injury (hypoxia/reoxygenation ...
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bioRxiv - Molecular Biology 2021Quote: ... and proteins were produced in the M9 media supplemented with 2 g/L [15N]NH4Cl (Cambridge Isotope Laboratories) and 3 g/L d-[13C6]glucose (Cambridge Isotope Laboratories) ...
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bioRxiv - Cancer Biology 2022Quote: ... the dried pellet was dissolved in 550 µl D2O containing 0.01 mg/ml Sodium 2,2-Dimethyl-2-Silapentane-5-Sulfonate (DSS; Cambridge Isotope). The samples were then vortexed and centrifuged at 16000 rpm for 12 min ...
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bioRxiv - Cell Biology 2023Quote: ... The media was exchanged for media with or without 5 mM 13C6-Glucose (Cambridge Isotope Laboratories Inc., CLM-1396) in HPLM or DMEM based MCF10A media for 2 h (for 13C6-Glucose media ...
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bioRxiv - Biochemistry 2023Quote: ... cells were grown in the standard HMI-11 medium supplemented with 5 mM 13C-U-glycerol (Cambridge Isotope Laboratories). For the experiment with Δfbp.sbp/RNAiPFK ...
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bioRxiv - Molecular Biology 2020Quote: ... protein was expressed in BL21/DE3 Escherichia coli cells using M9 minimal media supplemented with 15NH4Cl (Cambridge Isotope Laboratories) induced at an OD600 of 0.6 with 0.5 mM IPTG for 16 hours at 18°C ...
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bioRxiv - Biochemistry 2021Quote: ... 25.9 mM NaHCO3) supplemented with 5 mM glucose and 50 μM [U-13C]-succinic acid (Cambridge isotope CLM-1571-0.1). This buffer was pre-equilibrated at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... containing 0.11 mM of the internal standard DSS (sodium 2,2-dimethyl-2-silapentane-5-sulfonate, D6, 98%; Cambridge Isotope Laboratories) at pH 7.0 and vortex mixed for <1 min prior to transfer into 1.7 mm SampleJet NMR tubes ...
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bioRxiv - Biochemistry 2021Quote: ... 5 mM MgCl2, 0.5 mM EDTA, 0.5 mM ATP, 1 mM DTT, 10% glycerol [v/v], D2O [Cambridge Isotope Laboratories]) at 25°C and quenched by a 2-fold dilution with ice-cold quench buffer (0.8 M guanidine hydrochloride ...
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bioRxiv - Cell Biology 2022Quote: ... TKO and Glut 5 were gavaged with a mix of 1:1 universally labeled fructose (U-13C6, Cambridge Isotope Laboratories) and regular glucose at 1g/kg body weight ...
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bioRxiv - Immunology 2023Quote: ... On the final day all media conditions were replaced with 5 mM [U-13C]-fructose (Cambridge Isotope Laboratories, CLM-1553). Metabolites were extracted using 80% methanol 24 hours later ...
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bioRxiv - Biophysics 2021Quote: ... and the cell pellet was resuspended in 0.5L of M9 minimal media containing 15NH2Cl with either 13C-glucose or 5% 13C-glucose (Cambridge Isotope Laboratories). Incubation continued for about 1hr at 35 C when expression was induced with 1 mM IPTG ...
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bioRxiv - Microbiology 2022Quote: ... or 300 mL (algal cultures) of filtrate was collected in glass Erlenmeyer flasks and spiked with 5 μL of benzoate-d5 (98%, Cambridge Isotope Laboratries ...
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bioRxiv - Systems Biology 2022Quote: ... in a 100 mM sodium phosphate buffered solution with 0.11 mM sodium 2,2-dimethyl-2-silapentane-5-sulfonate (DSS-D6; 98%; Cambridge Isotope Laboratories, Inc.). Once dry ...
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bioRxiv - Cancer Biology 2023Quote: ... no-glucose and no-glutamine media was supplemented with 5 mM of 13C6 D-Glucose (Cambridge Isotope Laboratories, Inc., CLM-1396) and 4 mM unlabeled glutamine ...
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bioRxiv - Biochemistry 2019Quote: ... assay and immediately diluted (1:1) to a final protein concentration of 0.5 mg/mL with either 18O heavy (Cambridge Isotope Laboratories) or 16O light (Fisher ...
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bioRxiv - Biophysics 2023Quote: ... 15N-labeled and 15N-13C labeled (as required) TRBP2-dsRBD2 protein was prepared using 15NH4Cl and 13C-glucose (Cambridge Isotope Laboratories) as a sole source of nitrogen and carbon ...
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bioRxiv - Biophysics 2024Quote: ... to obtain the 15N uniformly single labelled protein or in a media containing 15NH4Cl (0.1% w/v) and 13C-labelled glucose (0.4% w/v) (Cambridge isotope Laboratories, Inc.) for 15N and 13C uniformly double labelled protein ...
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bioRxiv - Immunology 2021Quote: ... complete CTL:AIM-V labeling medium consisted of glucose-free CTL:AIM-V with 5% heat-inactivated human AB serum supplemented with 11 mM uniformly labeled 13C-glucose ([U-13C]glucose) (Cambridge Isotope Laboratories). Glucose-free ImmunoCult™-XF T Cell Expansion Medium (STEMCELL Technologies ...
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bioRxiv - Cell Biology 2023Quote: Mice were injected intraperitoneally with AZD 5 mg/kg, 2 h prior to intravenous tail vein injection with [U13C] succinate (10 mg/kg, 2 min) (Cambridge Isotope Laboratories). Intravenous injections were performed as a bolus over 20 s ...
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bioRxiv - Microbiology 2024Quote: ... 100 µL of each strain was transferred from the unlabeled glucose M9 media to 5 mL of M9 media with 50% 13C glucose (99% 13C, Cambridge Isotope Laboratory) and 50% natural abundance glucose and allowed to grow overnight at room temperature ...
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bioRxiv - Biophysics 2019Quote: ... and uniformly 15N- or 15N,13C-labeled proteins were purified from cells grown in M9 minimal media with 15N ammonium chloride and/or 13C D-glucose (Cambridge Isotope Labs) as the sole nitrogen and/or carbon source ...
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bioRxiv - Biochemistry 2021Quote: ... then 10% of bead volume was lysed with 1% Triton buffer for protein extracts and the remaining 90% was extracted in 80% methanol containing heavy labeled amino acid standards (Cambridge Isotope Labs) on a rotator shaker for 10□min at 4□°C.
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bioRxiv - Biochemistry 2023Quote: ... 15N-labeled protein samples were grown in 1L minimal media containing 3 g/L 15N ammonium chloride (Cambridge Isotope Laboratories, Tweksbury, MA) and unlabeled protein samples were grown in 1L super broth media ...