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Citations for Cambridge Isotope Labs :
101 - 150 of 169 citations for Recombinant Human ROR2 protein Fc tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... 15N-labeled SARS-CoV-2 nsp7 sample was expressed and purified by the same methods as previously described in our NMR assignment paper.20 The recombinant Escherichia coli Rossetta2 pLysS was first grown in a M9 minimal medium containing 1 g/L 15N4HCl (Cambridge Isotope Laboratories), 4 g/L 13C6-D-Glucose (Cambridge Isotope Laboratories ...
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bioRxiv - Neuroscience 2021Quote: ... The desalted peptides on the StageTips were subjected to dimethyl-labeling with isotope-labeled formaldehyde (13CD2O, ISOTEC, U.S.A.) and NaBD3CN (Cambridge Isotope Laboratories, U.S.A.) (heavy label ...
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bioRxiv - Neuroscience 2021Quote: ... Then samples were labeled with either heavy formaldehyde (formaldehyde containing the isotope 13C and deuterium (13CD2O from Cambridge Isotope Laboratories, Inc) to a final concentration of 60 mM or light formaldehyde (regular formaldehyde (12CH2O from Sigma ...
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bioRxiv - Biochemistry 2020Quote: ... with non-labeled Xyl6 (200 µg) and Ac-CoA (4 mM) in 100 mM potassium phosphate buffer in D2O (99.9%; Cambridge Isotope Laboratories) for 6 h at room temperature ...
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bioRxiv - Biochemistry 2020Quote: C9S SqrR samples for backbone assignments were isotopically labeled using published procedures47 with all isotopes for NMR experiments purchased from Cambridge Isotope Laboratories ...
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bioRxiv - Cell Biology 2021Quote: ... and then harvested with 1mL/well of 80% methanol (on dry ice) with 500 nM 13C- and 15N-labeled amino acids (Cat#MSK-A1-1.2, Cambridge Isotope Laboratories) as internal standards ...
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bioRxiv - Cancer Biology 2020Quote: ... TIF was mixed 1:1 with a solution of isotopically labeled glucose at a known concentration (Cambridge Isotope Laboratories, CLM-1396). Samples were extracted in 300 µl of ice cold HPLC grade methanol ...
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bioRxiv - Biochemistry 2021Quote: ... with inclusion of 10 µL 100 µg/mL labeled (13C) nicotinamide adenine dinucleotide (NAD+) (Cambridge Isotope Laboratories, Inc., CLM-10671-PK) and 10 µL of 100 µg/mL norvaline (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... each of which was 25% labeled with 13C (i.e. mixed 1:4 with 25% H13CO3, 13C12H6O12, or CH313CO2, respectively; Cambridge Isotope Laboratories). All saltwater media was adapted from the Halohandbook and contained NaCl ...
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bioRxiv - Microbiology 2021Quote: ... A pulse of 0.025% (w/v) stable isotope-labeled lysine derivative 13C615N2 L-lysine (“heavy” lysine, Lys8, K8, Cambridge Isotope Laboratories) was then added in the presence of ampicillin and 1 – 2 mL of cultures were harvested in intermittent time intervals (10min - 24h ...
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bioRxiv - Microbiology 2022Quote: ... G7021 and for labeled glucose we used either D-glucose-U-13C (99%, Cambridge Isotope Laboratories, cat no CLM-1396-10) or D-glucose-2-13C glucose (99% ...
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bioRxiv - Systems Biology 2019Quote: ... for “heavy” (“H”)-labeled cells 13C615N4-L-arginine (89 mg/L) and 13C615N2-L-lysine (154 mg/L)(Cambridge Isotope Laboratories). Cells were split into each SILAC formulation and passaged six times to allow for complete incorporation of amino acid isotopologues.
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bioRxiv - Cell Biology 2021Quote: ... that cannot form lysine-linked polySUMO chains were grown for at least ten divisions in synthetic complete media supplemented either with unlabeled (Lys0 and Arg0; light) or heavy isotope-labeled amino acids (Lys8 and Arg10; heavy) from Cambridge Isotope Laboratories ...
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bioRxiv - Immunology 2022Quote: ... and re-cultured (2.5 × 106 cells/well in 24-well plates) for indicated times in medium containing 13C-labeled metabolites (Cambridge Isotope Laboratories) at the following concentrations ...
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bioRxiv - Cancer Biology 2023Quote: GMP clones were lysed in 100 µl ice-cold 80% methanol in water and polar metabolites were extracted using methanol-chloroform phase separation (1 ml methanol containing 2.5 µM of an internal standard (13C-, 15N-labeled amino acid mix; Cambridge Isotope Laboratories), 500 µl water ...
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bioRxiv - Cell Biology 2020Quote: ... L amino acids were substituted for the same concentrations of heavy isotope–labeled (H) amino acids 13C6-15N2 L-Lys:2HCl and 13C6-15N4 L-Arg (Cambridge Isotope Laboratories). For immature dendritic cells (Dc ...
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bioRxiv - Cell Biology 2022Quote: ... Polar metabolites were extracted in 80 % methanol containing 15N and 13C fully-labeled amino acid standards (MSK-A2-1.2, Cambridge Isotope Laboratories, Inc). Extracts were shaken for 10 min with a vortexer ...
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bioRxiv - Developmental Biology 2021Quote: ... /water (2:2:1) containing isotope-labeled internal standards ([U-13C6]glucose; [U-13C3]lactate; [U-13C5]glutamine; and [U-13C5]glutamate; Cambridge Isotope Laboratories) were spiked into media samples ...
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bioRxiv - Biochemistry 2021Quote: ... The labeled M9 minimal medium had the same composition except for the replacement of NH4Cl and glucose by [15N]NH4Cl and uniformly labeled [13C]glucose at the same concentrations (99% labeling for both compounds; Cambridge Isotope Laboratories). The growth media were sterilized by filtration (Millex-LG 0.2 µm ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% FBS and heavy isotope-labeled amino acids (L-Lysine 13C6, L-Arginine 13C6) (CLM-2247-H-1, CLM-2265-H-1, Cambridge Isotope Laboratories). DAC-treated cells grown in the regular medium were considered as SILAC light counterparts.
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bioRxiv - Cell Biology 2021Quote: ... and 500 μl of ice-cold 80% methanol in water with 250nM 13C/15N labeled amino acid standards (MSK-A2-1.2: Cambridge Isotope Laboratories, Inc.) was added to each well ...
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bioRxiv - Microbiology 2021Quote: ... 2 mL of LC-MS grade water containing 0.1 μL of MSK-A2-1.2 stable isotope-labeled amino acid standards (Cambridge Isotope Laboratories, Tewksbury, MA) was added before vortexing for 1 minute ...
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bioRxiv - Cancer Biology 2022Quote: ... H2O/ACN/MeOH supplemented with stable isotopic labeled amino acids solution at 1 nmol/mL (MSK-A2-1.2, Cambridge Isotope Laboratories, USA).
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bioRxiv - Immunology 2023Quote: ... activated T cells were washed with PBS and re-cultured in TCM (lacking glucose and serine) containing 10% dialyzed FBS and uniformly labeled [13C]-Glucose (Cambridge Isotope Laboratories). T cells (5 x 106 per well in 6 well plates ...
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bioRxiv - Physiology 2023Quote: Metabolomics was performed on microglia by first washing microglia primary cell cultures with PBS and re-cultured in DMEM (lacking glucose) containing 10% dialyzed FBS and uniformly labeled [13C]-Glucose (Cambridge Isotope Laboratories). Microglia (2 x 106 per well in 6 well plates ...
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bioRxiv - Microbiology 2023Quote: ... to 13CH4 or CD3H was tracked by incubating active enrichment cultures in the presence of 20% labeled substrate (98%; Cambridge Isotope Laboratories). Incubations were carried out in 30 mL culture volumes in 60 mL serum bottles with 8% v/v inoculum ...
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bioRxiv - Cancer Biology 2023Quote: ... Media was aspirated from cells and then washed rapidly in ice cold bank saline followed by addition of 500 mL ice-cold 80% methanol in water containing 500 nM each of 17 isotopically labeled 13C/15N amino acids (Cambridge Isotope Laboratories). Alternatively ...
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bioRxiv - Cell Biology 2024Quote: ... 10 µL supernatant was added 40 µL solution of stable isotope-labeled internal standards (phenylalanine-D5, tyrosine-D4, valine-D8, methionine-D3, lysine-13C6-15N2, and arginine 13C6-15N4; Cambridge Isotope Laboratories) and 180 µL solvent (water with 0.2% heptafluorobutyric acid) ...
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bioRxiv - Microbiology 2023Quote: The amino acids were extracted with water spiked with 50 µM of an isotopically labeled mix of 17 amino acids (Cambridge Isotope Laboratories). The amino acids were separated on a Imtakt Intrada Amino Acid column (3 mm × 150 mm ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were quenched in 1 mL pre-chilled −80°C 80/20 methanol:water (v/v) and spiked with 50µL 1µM isotope labeled TCA cycle mix (Cambridge Isotope Laboratories MSK-TCA-A) pre-diluted in 80/20 methanol:water and 50µL 0.02 ng/µL Propionyl-L-carnitine-(N-methyl-d3 ...
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bioRxiv - Cancer Biology 2020Quote: ... 10μL of media was mixed with 10μL of a mixture of isotopically labeled metabolites and amino acids of known concentrations (Cambridge Isotope Laboratories, Tewksbury, MA) and extracted with 600μL ice-cold HPLC grade methanol ...
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bioRxiv - Cancer Biology 2022Quote: ... polar metabolites were extracted from 5µL IF samples using 45µL 75:25:0.1 HPLC grade acetonitrile:methanol:formic acid extraction mix into which a mixture of isotopically labeled amino acids of known concentrations (Cambridge Isotope Laboratories, MSK-A2-1.2) was added ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HB101 bacteria were cultured overnight in cholesterol-free medium (10 g/L ether-extracted peptone in DMEM) and prepared as 10× concentrates supplemented with 140 μM naturally labeled (i.e., 12C) or 13C3 (Cambridge Isotope Laboratories, Tewksbury, MA) labeled cholesterol ...
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bioRxiv - Cancer Biology 2022Quote: ... the cell pellet was resuspended into 1 mL of ice-cold 2:2:1 MeOH: ACN: H2O (v/v/v) containing 10 μM stable isotope-labeled canonical amino acid mix (Cambridge Isotope Laboratories, Inc) as internal standards ...
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bioRxiv - Molecular Biology 2022Quote: Histones were prepared for MS analysis using derivatization with propionic anhydride as previously described (Lund et al., 2019) except that isotopically labeled propionic anhydride (D10, 98%, Cambridge Isotope Laboratories Inc.) was used to differentiate endogenous (light ...
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bioRxiv - Microbiology 2023Quote: ... were prepared via protein precipitation with the addition of 9 volumes of 74.9:24.9:0.2 v/v/v acetonitrile/methanol/formic acid containing stable isotope-labeled internal standards (valine-d8, Sigma-Aldrich; and phenylalanine-d8, Cambridge Isotope Laboratories). The samples were centrifuged (10 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... The tissue powder was then weighed into pre-chilled tubes and extracted with methanol (containing 500 nM each of 17 isotopically labeled 13C/15N amino acids (Cambridge Isotope Laboratories, Inc.)) ...
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bioRxiv - Biophysics 2022Quote: ... then centrifuging and resuspending in M9 minimal medium containing 2 g/L unlabeled glucose and 1 g/L 15N-labeled ammonium acetate (Cambridge Isotope Labs, Tewksbury, MA) and induced with 1 mM IPTG ...
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bioRxiv - Cancer Biology 2022Quote: ... Dried samples were rehydrated with 2:1 methanol:water into which a mixture of isotopically labeled amino acids of known concentrations (Cambridge Isotope Laboratories, MSK-A2-1.2) was added ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... were diluted using 90 µL of 74.9:24.9:0.2 v/v/v acetonitrile/methanol/formic acid containing stable isotope-labeled internal standards (valine-d8, Isotec; and phenylalanine-d8, Cambridge Isotope Laboratories; Andover, MA). The samples were centrifuged (10 min ...
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bioRxiv - Cell Biology 2020Quote: ... 500 μl of each cell lysate sample was blown to dryness with N2 and reconstituted with 150 μL of an Acetonitrile/H2O (2:1) solution containing stable isotope-labeled internal standards: tyrosine-d2 and lactate-13C3 (Cambridge Isotope Lab, Tewksbury, MA). Centrifuged the cell lysate at 10,000 g for 20 minutes ...
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bioRxiv - Cancer Biology 2023Quote: 5 μL of adult bovine serum or ftABSB was mixed with 45 μL of HPLC-grade methanol:water (80:20) including 500 nM 13C/15N labeled amino acids (Cambridge Isotope Laboratories, MSK-CAA-1). Samples were vortexed for 15 min at 4°C and insoluble material was sedimented by centrifugation at 16,000 x g for 10 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Taurine concentrations were then measured via LC-MS and analyzed the area of the exact mass for the corresponding ions of the targets as well as the intracellular labeled isotopic taurine levels (13C2, 99%; 15N, 98%, Cambridge isotope #CNLM-10253-PK).
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bioRxiv - Cancer Biology 2023Quote: ... 5 μL of media was mixed with 45 μL of HPLC-grade methanol:water (80:20) including 500 nM 13C/15N labeled amino acids (Cambridge Isotope Laboratories, MSK-CAA-1). Samples were then vortexed for 15 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: 100 µL of each CSF sample was spiked with 5 µL of a mixture of stable isotope-labeled standards (Cambridge Isotope Labs, Tewksbury, MA). An aliquot of pre-chilled methanol was added to each sample for a final composition of 80% methanol to extract metabolites and precipitate proteins ...
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bioRxiv - Cell Biology 2023Quote: ... L-LYSINE:2HCL (13C6, 99%;15N2, 99%) and stable isotope-labeled (heavy) L-ARGININE:HCL (13C6, 99%; 15N4, 99%) (Cambridge Isotope Labs, Inc. Andover, MA). Light SILAC medium was generated by adding unlabeled L-LYSINE:2HCL (final concentration 146.0 mg/mL ...
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bioRxiv - Microbiology 2019Quote: ... Duplicate microcosms were fed with a 1 M solution of either 12C sodium butyrate or 13C-labeled sodium butyrate (>98% atom purity, Cambridge Isotope Laboratories, Tewksbury, MA, USA) to reach an initial butyrate concentration of 40 mM ...
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bioRxiv - Epidemiology 2019Quote: ... using 90 μL of acetonitrile/methanol/formic acid (74.9:24.9:0.2 v/v/v) containing stable isotope-labeled internal standards (valine-d8, Sigma-Aldrich; St. Louis, MO; and phenylalanine-d8, Cambridge Isotope Laboratories; Andover, MA). The samples were centrifuged (10 min ...
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Metabolic function in aging retina and retinal pigment epithelium remains robust despite vision lossbioRxiv - Biochemistry 2021Quote: Either 5 mM U-13C glucose alone or 5 mM unlabeled glucose and 2 mM U-13C glutamine U-13C metabolic tracers (99% isotopic purity) included 13C labeled D-Glucose and L-Glutamine (Cambridge Isotope Laboratories, Inc; Tewksbury, MA). Incubations ...
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bioRxiv - Microbiology 2022Quote: ... were resuspended in 300 μL of LC/MS grade methanol containing a mixture of 17 isotope-labeled amino acids (200 nM, Cambridge Isotope Laboratories, MSK-A2-1.2). To this solution ...