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Citations for Cambridge Isotope Labs :
1 - 50 of 192 citations for Recombinant Human Programmed Cell Death 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... 15N-labeled SARS-CoV-2 nsp7 sample was expressed and purified by the same methods as previously described in our NMR assignment paper.20 The recombinant Escherichia coli Rossetta2 pLysS was first grown in a M9 minimal medium containing 1 g/L 15N4HCl (Cambridge Isotope Laboratories), 4 g/L 13C6-D-Glucose (Cambridge Isotope Laboratories ...
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bioRxiv - Cancer Biology 2021Quote: ... PaTu-8902 and MIAPaCa-2 iDox-shGOT2.1 cells were cultured in media containing 1 mM unlabelled glucose and 1 mM U13C-Pyruvate (Cambridge Isotope Laboratories) for 16 hours.
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bioRxiv - Biophysics 2020Quote: ... The rinsed cells were then used to inoculate 1 L of M9 medium supplemented with 1 g/L 15N-NH4Cl (Cambridge Isotope Labs), 4 g/L dextrose ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were incubated in the media containing [U-13C]glucose (Cambridge Isotope Laboratories, CLM-1396-1) or [U-13C]glutamine (Cambridge Isotope Laboratories ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell were depleted of methionine for 1h and then 1 mM L-azidohomoalanine (AHA, Cambridge Isotope Laboratories) was added to the media with a dose of CDCexo (103 EV/ cell ...
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bioRxiv - Cancer Biology 2019Quote: ... 15-20E + 6 cells in light SILAC media were treated with drug compound and cells in heavy SILAC media (L-Lysine-13C6,15N2, L-Arginine-13C6,15N4 (Cambridge Isotope, CNLM-291-H-1, CNLM-539-H-1) were treated with DMSO for 24 hr ...
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bioRxiv - Biochemistry 2023Quote: ... The cells were scrapped in 50% methanol and 100 ug D4 alanine (Cambridge isotope lab DLM-250-1) and centrifuged at 13000 RPM for 5 minutes at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated with media containing the designated tracer γ-15N-glutamine (Cambridge Isotope Laboratories: NLM-557-1), or U-13C-glucose (Cambridge Isotope Laboratories ...
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bioRxiv - Systems Biology 2023Quote: ... T87 cells were cultured in 15N-enriched NT-1 liquid medium containing K15NO3 and 15NH415NO3 (Cambridge Isotope Labs), under identical culture conditions ...
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bioRxiv - Immunology 2022Quote: ... of the samples was used to scale the metabolite extraction to a ratio of 1×106 cells / 1 mL extraction solution [80% LCMS grade methanol (aq) with 500nM labelled amino acid internal standard (Cambridge Isotope Laboratories, Inc., Cat No. MSK-A2-1.2)] ...
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bioRxiv - Biochemistry 2023Quote: ... coli BL21(DE3)pLysS cells in standard M9 minimal medium using 1 g/l 15N NH4Cl (Cambridge Isotope Laboratories) as a sole nitrogen source ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were labeled with 40 mM isotopically distinct dimethyl formaldehyde (light, CH2O, for the parental RPE-1 cells (Cambridge Isotope Laboratories ...
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bioRxiv - Systems Biology 2023Quote: ... cells were grown in SCD medium for 24hrs and then pulsed with 1% 13C2-acetate (Cambridge Isotope Laboratories, CLM-440). Metabolites were extracted after 30 minutes of labelled acetate addition ...
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bioRxiv - Biophysics 2021Quote: ... The cells were collected by centrifugation and resuspended in M9 medium (1 L, 100% D2O) supplemented with 15NH4Cl (Cambridge Isotope Laboratories) and 12C6H12O6 as the sole nitrogen and carbon sources ...
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bioRxiv - Cell Biology 2019Quote: 3 μL of human plasma were spiked with 3 μL amino acid isotope labelled internal standards (Cambridge Isotope Laboratories, #MSK-A2-1.2) and extracted with 250 μL –20 °C methanol for 10 min and centrifuged at 4°C for 10 min at 15 000 g ...
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bioRxiv - Immunology 2021Quote: ... complete CTL:AIM-V labeling medium consisted of glucose-free CTL:AIM-V with 5% heat-inactivated human AB serum supplemented with 11 mM uniformly labeled 13C-glucose ([U-13C]glucose) (Cambridge Isotope Laboratories). Glucose-free ImmunoCult™-XF T Cell Expansion Medium (STEMCELL Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... activity in cultured melanoma cells was quantitated by measuring the transfer of 15N from [α–15N]glutamine (NLM-1016-1, Cambridge Isotope Laboratories) to 15NH4+ as described (37 ...
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bioRxiv - Molecular Biology 2021Quote: ... For the 15N isotope labeling cells were cultivated at 37 °C in M9 minimal medium with 1 g/L 15NH4Cl (Cambridge Isotope Laboratories, Inc.).
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bioRxiv - Microbiology 2023Quote: ... 250 µL of each human CVL sample was added to 750 µL of methanol containing 200 nM 13C18-OA (Cambridge Isotope Laboratories, Inc. #CLM-460-PK), incubated on ice for 15 min ...
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bioRxiv - Plant Biology 2023Quote: ... and 1 g/L KNO3 or 1 g/L K15NO3 (Cambridge Isotope Laboratories), pH 5.8 ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1 g/L KNO3 or 1 g/L K15NO3 (Cambridge Isotope Laboratories), pH 5.8 ...
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bioRxiv - Biophysics 2023Quote: ... Uniformly-labeled 13C 15N Sup35NM samples were prepared by growing BL21(DES)-Rosetta Escherichia coli in the presence of M9 media with 2 g L-1 D-glucose 1H,13C6 and 1 g L-1 of 15N ammonium chloride (Cambridge Isotope Labs, Cambridge, MA). Purified ...
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bioRxiv - Physiology 2020Quote: ... constant infusion (0.0085 mg•kg-1•min-1) of [ring-13C6]-L-phenylalanine (Cambridge Isotope Laboratories, Tewksbury, MA) from 1000 to the end of the protocol ...
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bioRxiv - Biochemistry 2019Quote: ... with 1 g 15NH4Cl (Cambridge Isotope Laboratories) as the sole nitrogen source ...
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bioRxiv - Bioengineering 2022Quote: ... 100 % 1-13C glucose (Cambridge Isotope Laboratories), or unlabelled glucose ...
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bioRxiv - Biochemistry 2022Quote: ... 1 g/L 15NH4Cl (Cambridge Isotope Laboratories) and 50 μg/mL kanamycin ...
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bioRxiv - Cancer Biology 2020Quote: ... Heavy lysine (1G: CLM-265-H-1) and arginine (1G: CNLM-291-H-1) were purchased from Cambridge Isotope Laboratories (CIL ...
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bioRxiv - Microbiology 2019Quote: ... 10 and 20 minutes and quenched in ice-cold acetonitrile:methanol (1:1) spiked with 0.1 mM 13C5,15N-L-glutamic acid as internal standard (Cambridge Isotope Laboratories). Samples were stored at -80 °C until RapidFire analysis ...
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bioRxiv - Cell Biology 2022Quote: ... TKO and Glut 5 were gavaged with a mix of 1:1 universally labeled fructose (U-13C6, Cambridge Isotope Laboratories) and regular glucose at 1g/kg body weight ...
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bioRxiv - Biochemistry 2019Quote: ... 13C6-glucose (Cambridge Isotope Laboratories CLM-1396-1), Vitamin B5-[13C6 15N2] (calcium pantothenate-[13C6 15N2] ...
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bioRxiv - Cancer Biology 2020Quote: ... 99% (Cambridge Isotope Laboratories, Inc., CLM-1396-1) and 2 mL of bovine pituitary extract ...
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bioRxiv - Biophysics 2021Quote: ... [1-13C]pyruvate (Cambridge Isotope Labs, Massachusetts, USA) with 15mM Ox063 (Oxford Instruments ...
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bioRxiv - Cancer Biology 2020Quote: ... TIF was mixed 1:1 with a solution of isotopically labeled glucose at a known concentration (Cambridge Isotope Laboratories, CLM-1396). Samples were extracted in 300 µl of ice cold HPLC grade methanol ...
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bioRxiv - Microbiology 2020Quote: ... irregularis was cultured in modified SC solid medium supplied with 1 mM neutralized [1-13C]myristic acid (Cambridge Isotope Laboratories, Inc.) for 2.5 months ...
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bioRxiv - Biochemistry 2019Quote: ... assay and immediately diluted (1:1) to a final protein concentration of 0.5 mg/mL with either 18O heavy (Cambridge Isotope Laboratories) or 16O light (Fisher ...
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bioRxiv - Plant Biology 2019Quote: ... or 15N MS medium (1/2 MS without nitrogen source [PhytoTechnology Laboratories], 15NH415NO3 [0.5 g/L, NLM-390-1, Cambridge Isotope Laboratory] ...
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bioRxiv - Biochemistry 2021Quote: ... BioExpress cell growth media (Cambridge Isotope Laboratories Inc., Andover, MA) was added (2×100 mL of 10X U-15N ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% FBS and heavy isotope-labeled amino acids (L-Lysine 13C6, L-Arginine 13C6) (CLM-2247-H-1, CLM-2265-H-1, Cambridge Isotope Laboratories). DAC-treated cells grown in the regular medium were considered as SILAC light counterparts.
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bioRxiv - Microbiology 2023Quote: ... or medium (84 mg L−1 13C6 L-arginine and 146 mg L−1 2H4 L-lysine) SILAC amino acids (Cambridge Isotope Laboratories). Medium SILAC labeled HeLa or HeLa S3 cells were used as spike-in control ...
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bioRxiv - Biochemistry 2023Quote: ... To the dried peptide, 300 μL of a 1:1 mixture 12 M DCl (35% w/v, Aldrich) and D2O (Cambridge Isotope Laboratory) containing 3% phenol (w/v ...
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bioRxiv - Immunology 2024Quote: ... the media was changed to DMEM media containing 1% dialyzed FBS or 1% dialyzed FBS supplemented with 2mM [U-13C8]-octanoate (Cambridge Isotope Laboratories). After 24h ...
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bioRxiv - Systems Biology 2021Quote: ... and 1 mM γ[18O4]-ATP (Cambridge Isotope Laboratory) in 30 °C for 1 hour ...
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bioRxiv - Plant Biology 2022Quote: ... or 1 mM 13C-ring labeled phenylalanine (Cambridge Isotope Laboratories ...
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bioRxiv - Microbiology 2023Quote: ... and [U-13C]1-propanol (Cambridge Isotope Laboratories, Inc.). Ethyl [U-13C]2-hydroxybutyrate (Cambridge Isotope Laboratories ...
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bioRxiv - Biochemistry 2021Quote: ... 13C-1 labelled polyM was produced from fermentation of the same strain using 13C-1 (99 % 13C) D-fructose (Cambridge Isotope Laboratories, USA). This substrate was further subjected to acid hydrolysis as described elsewhere (39 ...
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bioRxiv - Immunology 2024Quote: ... and labeled (U-13C6, Cambridge Isotope Laboratories CLM-1396-1) or unlabeled glucose (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... 99% [2,3-13C] sodium succinate or an equimolar molar 1:1 mixture of [U-13C] sodium succinate (obtained from Cambridge Isotope Laboratories, Inc., Andover, MA, USA) and naturally labelled sodium succinate.
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bioRxiv - Cancer Biology 2023Quote: ... 10 µL of urine was mixed with 90 µL of methanol: water (1:1) and internal standards (Labeled tryptophan (CNLM-2475-H-PK, Cambridge Isotope Laboratories, Inc., Andover, MA), zeatine and inosine ...
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Domain-specific quantification of prion protein in cerebrospinal fluid by targeted mass spectrometrybioRxiv - Neuroscience 2019Quote: ... coli in 15N cell growth medium (Cambridge Isotope Laboratories CGM-1000-N) induced with 15N auto-induction medium (Millipore 71759-3) ...
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bioRxiv - Biochemistry 2023Quote: ... 10 mL Bioexpress Cell Growth Media (Cambridge Isotope Laboratories, Tewksbury, MA, USA), 5 mL Gibco™ MEM Vitamin Solution (100x) ...