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Citations for Cambridge Isotope Labs :
51 - 100 of 125 citations for Prestained Protein Peptide Standards since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... metabolites were extracted with 80:20 methanol:water (−80°C) containing stable isotope-labeled internal standard [1,2,3,4-13C4]α-ketoglutaric acid (Cambridge Isotope Laboratories) and incubated at −80°C for 1 hr as described previously63,64 ...
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bioRxiv - Microbiology 2019Quote: ... 10 and 20 minutes and quenched in ice-cold acetonitrile:methanol (1:1) spiked with 0.1 mM 13C5,15N-L-glutamic acid as internal standard (Cambridge Isotope Laboratories). Samples were stored at -80 °C until RapidFire analysis ...
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bioRxiv - Cell Biology 2019Quote: ... and 100 μL ice-cold H2O containing amino acid isotope labelled internal standards (Cambridge Isotope Laboratories, #MSK-A2-1.2). An aliquot of tissue homogenate (50 μL ...
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bioRxiv - Cancer Biology 2022Quote: ... Dried pellet from unlabeled samples was mixed with [U13C]-D/L-2HG (internal standard for unlabeled samples, Cambridge isotope laboratories ...
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bioRxiv - Cell Biology 2022Quote: ... 600 μl LC/MS grade methanol containing 500 nM internal standards (Amino Acid Metabolomics Mix; Cambridge Isotope Laboratories, Inc.) was added to each well ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Samples were thawed on ice and phthalates were extracted from a 10 μL aliquot via vigorous vortexing for 30 min at 4°C in the presence of 5:3:2 methanol:acetonitrile:water (240 μL) containing an isotope labeled standard (1 μM DEHP ring-1,2-13C2, Cambridge Isotope Laboratories). Extraction supernatants were clarified via centrifugation at 18,000 rpm for 10 min at 4°C and analyzed immediately by ultra high-pressure liquid chromatography coupled to mass spectrometry (UHPLC-MS) ...
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bioRxiv - Biochemistry 2023Quote: ... coli BL21(DE3)pLysS cells in standard M9 minimal medium using 1 g/l 15N NH4Cl (Cambridge Isotope Laboratories) as a sole nitrogen source ...
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bioRxiv - Biochemistry 2023Quote: ... cells were grown in the standard HMI-11 medium supplemented with 5 mM 13C-U-glycerol (Cambridge Isotope Laboratories). For the experiment with Δfbp.sbp/RNAiPFK ...
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bioRxiv - Bioengineering 2021Quote: ... was used as a chemical shift and concentration reference standard by adding 333.3 μl of 1.0 M DSS-D6 (Cambridge Isotope Laboratory) stock solution to the buffer for a final DSS concentration of 1/3 mM ...
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bioRxiv - Biochemistry 2021Quote: ... containing 0.11 mM of the internal standard DSS (sodium 2,2-dimethyl-2-silapentane-5-sulfonate, D6, 98%; Cambridge Isotope Laboratories) at pH 7.0 and vortex mixed for <1 min prior to transfer into 1.7 mm SampleJet NMR tubes ...
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bioRxiv - Systems Biology 2019Quote: ... 20nM isotope-labeled fatty acid standards (U-13C-C16:0, U-13C-C18:1, U-13C-C18:2; Cambridge Isotope) were added before saponification as internal standards ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... in borate buffer (0.1 M, pH= 8) and 10 μL of the internal standard (200 ppb NaS13C15N) (Cambridge Isotope Labsyes). To minimize pipetting errors ...
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bioRxiv - Cancer Biology 2023Quote: ... Metabolites were extracted in 1 mL ice-cold 80% methanol containing 500 nM internal amino acid standards (Cambridge Isotope Laboratories). Following a 10 min vortex and centrifugation at 21130 g for 3 min at 4°C ...
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bioRxiv - Physiology 2023Quote: ... The samples were reconstituted in 125 µl of an acylcarnitine internal standard mix (NSK-B reference mix, Cambridge Isotope Laboratories) prepared in acetonitril:methanol [3:1] ...
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bioRxiv - Plant Biology 2023Quote: ... The following isotope-labelled standards were added at 1 pmol per sample: 13C6-IAA (Cambridge Isotope Laboratories, Tewksbury, MA, USA); 2H4-SA (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... and resuspended in different volumes of water containing labeled non-standard amino acid mix (Cambridge Isotope Laboratories, MSK-NCAA-1) to account for differences in starting tissue weight.
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bioRxiv - Cancer Biology 2023Quote: ... 50 μL of internal standard solution (U-13C-15N-cysteine, catalog no: CNLM-3871-H-PK, Cambridge Isotope Laboratories catalog) was added ...
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bioRxiv - Genetics 2024Quote: ... Polar metabolites were extracted in 500μL ice-cold 80% methanol containing amino acid standards (Cambridge Isotope Labs, MSK-A2-1.2). Samples were vortexed at 4°C for 10 minutes and centrifuged for 10 minutes at 20000xg at 4°C before supernatant was collected and dried via nitrogen evaporation ...
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bioRxiv - Cell Biology 2021Quote: ... The DPC-bound peptide sample was prepared by dissolving lyophilized peptide in PBS supplemented with 7% D2O with 0.7% perdeuterated d38-DPC (Cambridge Isotope Laboratories) at a final concentration of ~ 2 mM ...
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bioRxiv - Physiology 2022Quote: ... ice-cold 400μL saline and ice-cold dH2O containing amino acid isotope labelled internal standards (Cambridge Isotope Laboratories, #MSK-A2-1.2). An aliquot of homogenate (50μL ...
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bioRxiv - Biochemistry 2020Quote: An internal standard stock mixture was prepared using the following compounds and concentrations: citric acid-d4 (Cambridge Isotope Laboratories, DLM-3487), 225 μM ...
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bioRxiv - Plant Biology 2020Quote: ... 200 μL of 65% isopropanol /35% 0.2 M pH 7.0 imidazole buffer (Chen et al. 1988) was added with [13C6] IAA internal standard (Cambridge Isotope Laboratories Inc.), and samples were extracted on ice for 1 h ...
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bioRxiv - Cancer Biology 2022Quote: ... and SVVESVKFL+7 were synthesized at the MIT-Koch Institute Swanson Biotechnology Center in Biopolymers and Proteomics Facility using standard Fmoc chemistry using an Intavis model MultiPep peptide synthesizer with HATU activation and 5 μmol chemistry cycles as previously described.(24) Standard Fmoc amino acids were procured from NovaBiochem and Fmoc-Leu (13C6, 15N) was obtained from Cambridge Isotope Laboratories ...
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bioRxiv - Biochemistry 2021Quote: ... Extraction solution contained 80:20 methanol:water with 1:2500 dilution of a heavy amino acid mix standard (MSK-A2, Cambridge Isotope Laboratories); derivatization solution is described below ...
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bioRxiv - Cancer Biology 2021Quote: ... and polar metabolites extracted in 1 mL of cold 80% methanol containing internal standards (MSK-A2-1.2, Cambridge Isotope Laboratories, Inc.) by scraping the cells in each well on ice and collecting the content in 1.5 mL tubes ...
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bioRxiv - Microbiology 2020Quote: ... Samples were extracted using cold extraction solution containing 80% methanol/20% water/0.2 uM heavy internal standard mix (MSK-A2-1.2 Cambridge Isotope Laboratories, Inc) using 2 million cells in 500 uL ...
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bioRxiv - Cancer Biology 2023Quote: GMP clones were lysed in 100 µl ice-cold 80% methanol in water and polar metabolites were extracted using methanol-chloroform phase separation (1 ml methanol containing 2.5 µM of an internal standard (13C-, 15N-labeled amino acid mix; Cambridge Isotope Laboratories), 500 µl water ...
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bioRxiv - Plant Biology 2023Quote: ... 200 µl of 65% isopropanol/35% 0.2 M imidazole extraction buffer pH 7.0 (Chen et al. 1988) and 5.0 µl of [13C6]-IAA internal standard (Cambridge Isotope Laboratories Inc.) were added ...
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bioRxiv - Cell Biology 2023Quote: ... of 80% methanol in LC–MS water containing 500 nM isotope-labelled amino acids used as internal standards (Cambridge Isotope Laboratories). Following extraction ...
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bioRxiv - Cancer Biology 2023Quote: ... Two transitions were optimised using an authentic standard of glycocholic acid (glycine-1-13C, CLM-191-PK, Cambridge Isotope Laobratories, UK), from the negative precursor ion (m/z 465 ...
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bioRxiv - Neuroscience 2023Quote: ... Chromatographic performance was quality controlled using a mixture of unlabeled standard amino acids and a mixture of chemically diverse compounds (Cambridge Isotope Laboratories ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were washed three times with 1 ml of cold 0.9% NaCl and scraped into 500µL of cold 80%v/v LC-MS grade methanol containing heavy amino acid internal standards (Cambridge Isotope Laboratories). After 10 minutes of vortexing at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... SRM transitions were detailed in the TraceFinder software and then verified with the Metabolomics Amino Acid Mix Standard (Cambridge Isotope Laboratories) for unique transitions ...
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bioRxiv - Biochemistry 2023Quote: ... To the dried peptide, 300 μL of a 1:1 mixture 12 M DCl (35% w/v, Aldrich) and D2O (Cambridge Isotope Laboratory) containing 3% phenol (w/v ...
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bioRxiv - Cell Biology 2019Quote: 3 μL of human plasma were spiked with 3 μL amino acid isotope labelled internal standards (Cambridge Isotope Laboratories, #MSK-A2-1.2) and extracted with 250 μL –20 °C methanol for 10 min and centrifuged at 4°C for 10 min at 15 000 g ...
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bioRxiv - Cell Biology 2022Quote: ... Polar metabolites were extracted in 80 % methanol containing 15N and 13C fully-labeled amino acid standards (MSK-A2-1.2, Cambridge Isotope Laboratories, Inc). Extracts were shaken for 10 min with a vortexer ...
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bioRxiv - Developmental Biology 2021Quote: ... /water (2:2:1) containing isotope-labeled internal standards ([U-13C6]glucose; [U-13C3]lactate; [U-13C5]glutamine; and [U-13C5]glutamate; Cambridge Isotope Laboratories) were spiked into media samples ...
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bioRxiv - Cell Biology 2021Quote: ... and 500 μl of ice-cold 80% methanol in water with 250nM 13C/15N labeled amino acid standards (MSK-A2-1.2: Cambridge Isotope Laboratories, Inc.) was added to each well ...
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bioRxiv - Plant Biology 2020Quote: ... 200 μL of 65% isopropanol /35% 0.2 M pH 7.0 imidazole buffer (Chen et al. 1988) was then added with [13C6] IAA internal standard (Cambridge Isotope Laboratories Inc.), and samples were extracted on ice for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... 2 mL of LC-MS grade water containing 0.1 μL of MSK-A2-1.2 stable isotope-labeled amino acid standards (Cambridge Isotope Laboratories, Tewksbury, MA) was added before vortexing for 1 minute ...
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bioRxiv - Cell Biology 2022Quote: ... Metabolites were extracted with 1 mL 80% MeOH pre-cooled to −80 °C containing 10 nmol [D8]-valine as an internal standard (Cambridge Isotope Labs). Insoluble material was removed by centrifugation at 21,000 ×g for 15 min at 4 °C ...
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bioRxiv - Physiology 2023Quote: ... of plasma together with standards (estradiol, Cerillant; testosterone, National Measurement Institute (NMI)) and quality control samples were fortified with deuterated d4-estradiol (Cambridge Isotope Lab) and d3-testosterone (NMI ...
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bioRxiv - Cell Biology 2024Quote: ... 10 µL supernatant was added 40 µL solution of stable isotope-labeled internal standards (phenylalanine-D5, tyrosine-D4, valine-D8, methionine-D3, lysine-13C6-15N2, and arginine 13C6-15N4; Cambridge Isotope Laboratories) and 180 µL solvent (water with 0.2% heptafluorobutyric acid) ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell pellets were resuspended in 1 mL of 80% methanol solution containing 500 nM internal standards (Metabolomics Amino Acid Mix, Cambridge Isotope Laboratories Inc.). Samples were vortexed at 4 degrees ...
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bioRxiv - Cell Biology 2020Quote: ... Data was normalized to 100 μg per sample and polar metabolites were extracted in a total volume of 275 μl of 40:40:20 (acetonitrile:methanol:water) with inclusion of internal standard d3N15-serine (Cambridge Isotope Laboratories, Inc. #DNLM-6863). Extracted samples were centrifuged at 10,000 × g for 10 min and an aliquot of the supernatant was injected onto LC/MS where metabolites were separated by liquid chromatography as previously described (111) ...
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bioRxiv - Microbiology 2023Quote: ... were prepared via protein precipitation with the addition of 9 volumes of 74.9:24.9:0.2 v/v/v acetonitrile/methanol/formic acid containing stable isotope-labeled internal standards (valine-d8, Sigma-Aldrich; and phenylalanine-d8, Cambridge Isotope Laboratories). The samples were centrifuged (10 minutes ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... were diluted using 90 µL of 74.9:24.9:0.2 v/v/v acetonitrile/methanol/formic acid containing stable isotope-labeled internal standards (valine-d8, Isotec; and phenylalanine-d8, Cambridge Isotope Laboratories; Andover, MA). The samples were centrifuged (10 min ...
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bioRxiv - Cell Biology 2020Quote: ... 500 μl of each cell lysate sample was blown to dryness with N2 and reconstituted with 150 μL of an Acetonitrile/H2O (2:1) solution containing stable isotope-labeled internal standards: tyrosine-d2 and lactate-13C3 (Cambridge Isotope Lab, Tewksbury, MA). Centrifuged the cell lysate at 10,000 g for 20 minutes ...
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bioRxiv - Genomics 2019Quote: ... Samples were spiked with 10 nM of 8-[15N5]oxoG internal standard with 288.9/173 mass transition (Cambridge Isotope Laboratories, Inc., Tewksbury, MA, USA). Separate samples were subjected to high-performance liquid chromatography (HPLC ...
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bioRxiv - Cancer Biology 2023Quote: 100 µL of each CSF sample was spiked with 5 µL of a mixture of stable isotope-labeled standards (Cambridge Isotope Labs, Tewksbury, MA). An aliquot of pre-chilled methanol was added to each sample for a final composition of 80% methanol to extract metabolites and precipitate proteins ...