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Citations for Cambridge Isotope Labs :
1 - 50 of 159 citations for Nnn Unlabeled 2 Mg Ml In Acetonitrile since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... in dry acetonitrile (10 ml) was added 2-bromoethanol-1,1,2,2-d4 (240 μl, Cambridge Isotope Laboratories) under argon ...
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bioRxiv - Cancer Biology 2022Quote: ... Dried pellet from unlabeled samples was mixed with [U13C]-D/L-2HG (internal standard for unlabeled samples, Cambridge isotope laboratories ...
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bioRxiv - Physiology 2023Quote: ... a canonical amino acid unlabeled standard mix (Cambridge isotope laboratories ...
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bioRxiv - Physiology 2023Quote: ... and a canonical amino acid unlabeled standard mix (Cambridge isotope laboratories ...
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bioRxiv - Microbiology 2024Quote: ... 100 µL of each strain was transferred from the unlabeled glucose M9 media to 5 mL of M9 media with 50% 13C glucose (99% 13C, Cambridge Isotope Laboratory) and 50% natural abundance glucose and allowed to grow overnight at room temperature ...
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bioRxiv - Plant Biology 2023Quote: RG-II (1-2 mg) was dissolved in D2O (0.2 mL, 99.9%; Cambridge Isotope Laboratories, Tewksbury, MA, USA) and placed in a 3 mm NMR tube 45 ...
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bioRxiv - Biophysics 2022Quote: ... then centrifuging and resuspending in M9 minimal medium containing 2 g/L unlabeled glucose and 1 g/L 15N-labeled ammonium acetate (Cambridge Isotope Labs, Tewksbury, MA) and induced with 1 mM IPTG ...
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bioRxiv - Cancer Biology 2022Quote: ... the dried pellet was dissolved in 550 µl D2O containing 0.01 mg/ml Sodium 2,2-Dimethyl-2-Silapentane-5-Sulfonate (DSS; Cambridge Isotope). The samples were then vortexed and centrifuged at 16000 rpm for 12 min ...
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bioRxiv - Cell Biology 2023Quote: Mice were injected intraperitoneally with AZD 5 mg/kg, 2 h prior to intravenous tail vein injection with [U13C] succinate (10 mg/kg, 2 min) (Cambridge Isotope Laboratories). Intravenous injections were performed as a bolus over 20 s ...
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bioRxiv - Microbiology 2021Quote: ... Egressed parasites were incubated in amino acid-free RPMI supplemented with 2 mg/ml algal [13C]amino acid mix (Cambridge Isotope Laboratories ...
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bioRxiv - Cell Biology 2023Quote: Lysine auxotrophic strains were grown in yeast nitrogen base (YNB) medium containing either 30 mg/ml L-Lysine or 30 mg/ml 13C6;15N2-L-Lysine (Cambridge Isotope Laboratories, USA). Cells were grown to logarithmic phase and up to 350 OD600 units were harvested of labeled cells ...
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bioRxiv - Cell Biology 2023Quote: ... lysine auxotrophic strains were grown in yeast nitrogen base (YNB) medium containing either 30 mg/ml L-Lysine or 30 mg/ml 13C6;15N2-L-Lysine (Cambridge Isotope Laboratories, USA). Cells were grown to logarithmic phase and the same amount of OD units of the control strain and the mutant strain were harvested and combined ...
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bioRxiv - Cell Biology 2023Quote: For whole cell proteomics lysine auxotrophic strains were grown in yeast nitrogen base (YNB) medium containing either 30 mg/ml L-Lysine or 30 mg/ ml 13C6;15N2-L-Lysine (Cambridge Isotope Laboratories, USA). Cells were grown to logarithmic phase and 1 OD unit of each strain was harvested ...
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bioRxiv - Cell Biology 2020Quote: ... 500 μl of each cell lysate sample was blown to dryness with N2 and reconstituted with 150 μL of an Acetonitrile/H2O (2:1) solution containing stable isotope-labeled internal standards: tyrosine-d2 and lactate-13C3 (Cambridge Isotope Lab, Tewksbury, MA). Centrifuged the cell lysate at 10,000 g for 20 minutes ...
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bioRxiv - Developmental Biology 2021Quote: ... 4mM either unlabeled or U-13C5-glutamine (Cambridge Isotope Laboratories cat. no. CLM-1822-H-0.1), 1mM Sodium Pyruvate ...
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bioRxiv - Biochemistry 2024Quote: ... mice were fed a liquid diet containing unlabeled glucose or U-13C-labeled glucose (Cambridge Isotope Laboratories) ad libitum for 48 h as previously described 52,38 ...
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bioRxiv - Microbiology 2021Quote: ... and 84 mg/mL [13C6,15N4]L-Arginine-HCl (Cambridge Isotope Laboratories) to DMEM Medium for SILAC (Thermo) ...
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bioRxiv - Microbiology 2019Quote: ... or 1 mM unlabeled methanol and 10 mM13C-labeled sodium bicarbonate (13C-labeled substrates provided by Cambridge Isotope Laboratories ...
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bioRxiv - Bioengineering 2021Quote: The NMR buffer solution was prepared by dissolving 928.6 mg of anhydrous NaH2PO4 and 320.9 mg of Na2HPO4 in 80 ml D2O (Cambridge Isotope Laboratory) in a volumetric flask ...
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bioRxiv - Developmental Biology 2021Quote: ... Tracer medium consisted of 25mM either unlabeled or U-13C6-glucose (Cambridge Isotope Laboratories cat. no. CLM-1396-0.5), 4mM either unlabeled or U-13C5-glutamine (Cambridge Isotope Laboratories cat ...
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bioRxiv - Cell Biology 2023Quote: ... Mice were then injected with [U13C] succinate (10 mg/kg, 2 min) (Cambridge Isotope Laboratories) via intravenous tail vein ...
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bioRxiv - Cell Biology 2021Quote: ... that cannot form lysine-linked polySUMO chains were grown for at least ten divisions in synthetic complete media supplemented either with unlabeled (Lys0 and Arg0; light) or heavy isotope-labeled amino acids (Lys8 and Arg10; heavy) from Cambridge Isotope Laboratories ...
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bioRxiv - Cancer Biology 2022Quote: ... 1×106 cells were plated in medium supplemented with 17.5 mM glucose (unlabeled glucose or 13C6-glucose [CLM-1396, Cambridge Isotope Laboratories]) or 2 mM glutamine (unlabeled glutamine or 13C5-glutamine [CLM-1822] ...
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bioRxiv - Neuroscience 2023Quote: ... Chromatographic performance was quality controlled using a mixture of unlabeled standard amino acids and a mixture of chemically diverse compounds (Cambridge Isotope Laboratories ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were preincubated in this media for 2 h until a metabolic steady state was reached at which time unlabeled glucose was replaced with [U-13C6]glucose (Cambridge Isotope Laboratories). Following the addition of 13C-label ...
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Multi cell line analysis of lysosomal proteomes reveals unique features and novel lysosomal proteinsbioRxiv - Molecular Biology 2020Quote: ... 181.2 mg/ml light or 13C615N2 lysine (Cambridge Isotope Laboratories, Inc., CNLM-291-H-1), and 87.8 mg/ml light or 13C615N4 arginine (Cambridge Isotope Laboratories ...
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bioRxiv - Biophysics 2024Quote: ... the cells were transferred to fresh LB medium for the overexpression of the unlabeled protein or to M9 minimal medium supplied with 15NH4Cl (Cambridge Isotope Laboratories Inc.) as the only nitrogen source for the labeled protein overexpression ...
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Multi cell line analysis of lysosomal proteomes reveals unique features and novel lysosomal proteinsbioRxiv - Molecular Biology 2020Quote: ... and 87.8 mg/ml light or 13C615N4 arginine (Cambridge Isotope Laboratories, Inc., CNLM-539-H-1) at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... or 0.5 mg/mL [1,2-13C2]acetate (all the reagents were purchased from Cambridge Isotope Laboratories, Inc) in a 50 mL sterile Falcon tube ...
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bioRxiv - Microbiology 2021Quote: Heavy medium (HM) was prepared by adding 146 mg/mL [13C6,15N2]L-Lysine-2HCl (Cambridge Isotope Laboratories) and 84 mg/mL [13C6,15N4]L-Arginine-HCl (Cambridge Isotope Laboratories ...
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bioRxiv - Microbiology 2023Quote: ... The dry MeOH extract (∼5 mg) was dissolved in 1 ml deuterated methanol (CD3OD; Cambridge Isotope Laboratories) for analysis by proton nuclear magnetic resonance (1H NMR ...
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bioRxiv - Cancer Biology 2024Quote: ... and then switched into normal DMEM containing 4.5 mg/mL D-Glucose (DLM-2062, Cambridge Isotope Laboratories) + 1mM Sodium Pyruvate (Catalog # ...
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bioRxiv - Microbiology 2022Quote: ... DNA with different levels of 13C enrichment was produced by varying the ratio of unlabeled glucose to uniformly-labeled 13C6-D-glucose (Cambridge Isotope Laboratories; CLM-1396; 99 atom %), e.g ...
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bioRxiv - Bioengineering 2020Quote: ... Rapamycin was extracted from blood and urine using a solution of methanol and acetonitrile (50:50 v/v) doped with rapamycin-D3 (Cambridge Isotope Laboratories) as an internal standard ...
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bioRxiv - Cell Biology 2021Quote: ... 15N2] lysine (100 mg/L) and [13C6,15N4] arginine (100 mg/L) (Cambridge Isotope Laboratories) (Heavy ...
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bioRxiv - Microbiology 2023Quote: ... were prepared via protein precipitation with the addition of 9 volumes of 74.9:24.9:0.2 v/v/v acetonitrile/methanol/formic acid containing stable isotope-labeled internal standards (valine-d8, Sigma-Aldrich; and phenylalanine-d8, Cambridge Isotope Laboratories). The samples were centrifuged (10 minutes ...
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bioRxiv - Biochemistry 2019Quote: ... assay and immediately diluted (1:1) to a final protein concentration of 0.5 mg/mL with either 18O heavy (Cambridge Isotope Laboratories) or 16O light (Fisher ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... were diluted using 90 µL of 74.9:24.9:0.2 v/v/v acetonitrile/methanol/formic acid containing stable isotope-labeled internal standards (valine-d8, Isotec; and phenylalanine-d8, Cambridge Isotope Laboratories; Andover, MA). The samples were centrifuged (10 min ...
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bioRxiv - Biochemistry 2020Quote: ... 15N2 HCl (190.59 mg/L) and L-proline (1000 mg/L) (Cambridge Isotope Laboratories, Andover, MA). Media components were mixed and sterile-filtered through a 0.22 um PES membrane filter (Millipore).
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bioRxiv - Cancer Biology 2022Quote: ... 84 mg/l 13C615N4 L-arginine and 175 mg/l 13C615N2 L-lysine (Cambridge Isotope Laboratories) until they were fully labeled ...
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Multi-omics characterization of partial chemical reprogramming reveals evidence of cell rejuvenationbioRxiv - Molecular Biology 2023Quote: ... Protein was precipitated from 10 µl of sample by addition of nine volumes of 74.9:24.9:0.2 v/v/v acetonitrile/methanol/formic acid containing internal standards (valine-d8, Sigma-Aldrich; St. Louis, MO; and phenylalanine-d8, Cambridge Isotope Laboratories; Andover, MA). Precipitated material was cleared by centrifugation and the supernatant was injected directly onto a 150 x 2 mm 3 µm Atlantis HILIC column (Waters ...
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bioRxiv - Developmental Biology 2019Quote: ... supplemented with 5 μl of heavy Lysine (Lys-8; stock: 30 mg/ml) (L-Lysine: 2HCl, U-13C6, 99%; U-15N2, 99%, Cambridge Isotope Laboratories, Inc.). The culture was incubated at 30°C for 24 hours ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... 84 mg/l 13C615N4 L-arginine and 175 mg/l 13C615N2 L-lysine (Cambridge Isotope Laboratories, Inc., Tewksbury, MA). All the cell lines were cultured under standard conditions (37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2019Quote: ... and the heavy amino acids L-Arginine 13C6 15N4 (147.5 mg/l) and L-Lysine 13C6 15N2 (91.25 mg/l; Cambridge Isotope Laboratories), or equal amounts of light arginine and lysine (Sigma) ...
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bioRxiv - Systems Biology 2019Quote: ... for “heavy” (“H”)-labeled cells 13C615N4-L-arginine (89 mg/L) and 13C615N2-L-lysine (154 mg/L)(Cambridge Isotope Laboratories). Cells were split into each SILAC formulation and passaged six times to allow for complete incorporation of amino acid isotopologues.
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bioRxiv - Biophysics 2023Quote: ... They were then resuspended in 500 ml of M9 media supplemented with 100 mg of a desired 15N-labeled or 13C-labeled L-amino acid (Cambridge Isotope Labs, Inc. or Sigma-Aldrich, Inc.) as well as the remaining 19 unlabeled amino acids ...
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bioRxiv - Cancer Biology 2021Quote: ... whereas the medium for the ‘heavy’ labelled cells contained 84 mg/l 15N L-arginine and 175 mg/l C615N2 L-lysine (Cambridge Isotope Laboratories). Cells were regularly tested for mycoplasma and MCF10DCIS.com cells authenticated ...
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bioRxiv - Microbiology 2023Quote: ... or medium (84 mg L−1 13C6 L-arginine and 146 mg L−1 2H4 L-lysine) SILAC amino acids (Cambridge Isotope Laboratories). Medium SILAC labeled HeLa or HeLa S3 cells were used as spike-in control ...
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bioRxiv - Cell Biology 2022Quote: ... 15N2 l-lysine (25 mg/L, Cambridge Isotope Laboratories) and reinoculated in heavy SCD ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were washed with PBS and fed 1 ml of culture medium supplemented with 2 mM [U-13C]glutamine (CLM-1822, Cambridge Isotope Laboratories) or 12.5 mM [1,2-13C]glucose (CLM-504-1 ...