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Citations for Cambridge Isotope Labs :
1 - 50 of 178 citations for Mouse Anti Dengue Virus Envelope Protein Serotypes 1 3 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... using a pET-46 tobacco etch virus (TEV) protease-cleavable N-terminal hexahistidine tag fusion protein in M9 media supplemented with 15NH4Cl (Cambridge Isotope Labs, Inc.). Nurr1 LBD was eluted against a 500 mM imidazole gradient through a Ni-NTA column ...
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bioRxiv - Biochemistry 2020Quote: ... 100 mM phosphate buffer (pH 7.4) with 1/3 mM DSS (Cambridge Isotope Laboratories), which was referenced to 0.0 ppm ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 1 g/L [U-13C]-glucose (Cambridge Isotope Laboratories, #110187-42-3), 10% dialyzed fetal bovine serum (R&D Systems ...
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bioRxiv - Biochemistry 2023Quote: ... 15N-labeled protein samples were grown in 1L minimal media containing 3 g/L 15N ammonium chloride (Cambridge Isotope Laboratories, Tweksbury, MA) and unlabeled protein samples were grown in 1L super broth media ...
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bioRxiv - Biochemistry 2019Quote: ... assay and immediately diluted (1:1) to a final protein concentration of 0.5 mg/mL with either 18O heavy (Cambridge Isotope Laboratories) or 16O light (Fisher ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Samples were thawed on ice and phthalates were extracted from a 10 μL aliquot via vigorous vortexing for 30 min at 4°C in the presence of 5:3:2 methanol:acetonitrile:water (240 μL) containing an isotope labeled standard (1 μM DEHP ring-1,2-13C2, Cambridge Isotope Laboratories). Extraction supernatants were clarified via centrifugation at 18,000 rpm for 10 min at 4°C and analyzed immediately by ultra high-pressure liquid chromatography coupled to mass spectrometry (UHPLC-MS) ...
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bioRxiv - Cell Biology 2020Quote: ... [3-13C]lactate (2mM; Cambridge Isotope Laboratory), [U-13C6]leucine (Cambridge Isotope Laboratory) ...
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bioRxiv - Cell Biology 2020Quote: ... [3-13C]pyruvate (2mM; Cambridge Isotope Laboratory), [3-13C]lactate (2mM ...
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bioRxiv - Biochemistry 2020Quote: ... and 3-2H-glucose (all from Cambridge Isotope), the isotopically labeled glucose was substituted for unlabeled glucose at the same concentration in RPMI culture media ...
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bioRxiv - Cancer Biology 2023Quote: ... 13C glucose (Cambridge Isotope Libraries 110187-42-3) was added to media lacking sodium pyruvate and glucose (Gibco 11966 ...
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bioRxiv - Molecular Biology 2021Quote: ... expression was carried out in M9 minimal media supplemented with 3 g/L (13C6, 99%)-D-glucose and/or 1 g/l (15N, 99%)-NH4Cl (Cambridge Isotope Laboratories). To generate isotopically labeled rad6-A126T ...
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bioRxiv - Biophysics 2020Quote: ... harvested at an OD600nm of 3 and resuspended in 2 L of M9 minimal medium with isotopic enrichment (1 g/L of 15NH4Cl and/or 3 g/L of 13C6-glucose, Cambridge Isotope Laboratories). After 30-45 min ...
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bioRxiv - Biophysics 2024Quote: ... The cells were used to inoculate 2L of H2O-based M9 media supplemented with 3 g L-1 of [U-13C6] d-glucose (Cambridge Isotope Laboratories, CIL) and 2 g L-1 of [15N] ammonium chloride (Cambridge Isotope Laboratories ...
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bioRxiv - Cell Biology 2019Quote: 3 μL of human plasma were spiked with 3 μL amino acid isotope labelled internal standards (Cambridge Isotope Laboratories, #MSK-A2-1.2) and extracted with 250 μL –20 °C methanol for 10 min and centrifuged at 4°C for 10 min at 15 000 g ...
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bioRxiv - Immunology 2024Quote: ... Labeled ([3-13C]-Lactate, Cambridge Isotope Laboratories CLM-1578) or unlabeled lactate (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... and 3 g/L d-[13C6]glucose (Cambridge Isotope Laboratories). The expression was induced by 1 mM IPTG after cells reached the density of ~2 OD600 ...
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bioRxiv - Cancer Biology 2022Quote: 13C4 3-hydroxybutyrate (Cambridge Isotope Laboratory, Andover, MA, CLM-3853)
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bioRxiv - Synthetic Biology 2023Quote: ... Deuterated indole-3-acetic acid (d7-IAA, Cambridge Isotope Laboratories) was used as an internal standard at a concentration of 75 nM in all samples and standards ...
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bioRxiv - Evolutionary Biology 2020Quote: ... for “medium” (M) labeling and (3) 13C6 15N2 L-Lysine (Cambridge Isotope Laboratories) for “heavy” (H ...
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bioRxiv - Bioengineering 2021Quote: ... 5 μL of 100/3 mM DSS-D6 in deuterium oxide (Cambridge Isotope Laboratories) were added to 1.7 mm NMR tubes (Bruker BioSpin) ...
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bioRxiv - Biochemistry 2021Quote: ... with inclusion of 3 µL 100 µM labeled (13C,15N) amino acid standard mix (Cambridge Isotope Laboratories ...
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bioRxiv - Molecular Biology 2022Quote: ... and 60 mg/L of alpha-ketoisovaleric acid (13C5, 98%; 3-D1, 98%, Cambridge Isotope Laboratory). All the recombinant protein expressions were performed at 37°C during 4 hours in presence of 1 mM IPTG.
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bioRxiv - Biophysics 2022Quote: ... and 70 mg/L sodium α-ketobutyrate (2H-3, 13C-methyl; Cambridge Isotope Laboratories, Tewksbury, MA). EmrE was purified also as previously reported (8 ...
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bioRxiv - Cancer Biology 2020Quote: ... [3-13C] serine (CLM-1572) and [U-2H]betaine (DLM-407-PK) were purchased from Cambridge Isotope Laboratories and stored in stocks of 10 mg/mL at −20°C ...
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bioRxiv - Biochemistry 2021Quote: ... protein was expressed in M9 minimal media with 15N-NH4Cl (Cambridge Isotope labs) as the sole nitrogen source instead of 2xYT media ...
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bioRxiv - Neuroscience 2020Quote: ... with a pH of 7.4 containing 6.15 mM NaN3 and 4.64 mM sodium 3-[trimethylsilyl] d4-propionate (Cambridge Isotope Laboratories) was prepared ...
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bioRxiv - Neuroscience 2023Quote: ... 5–10 μL of CSF or serum was extracted in 4:6:3 chloroform:methanol:water mixture supplemented with isotopically labeled T3 and T4 (at 100 nM, Cambridge Isotope Laboratories ...
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bioRxiv - Microbiology 2023Quote: ... We spiked 13C and 15N labeled algal AAs or protein (Cambridge Isotope Laboratories, Inc., MA) into late-log phase ...
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bioRxiv - Biochemistry 2019Quote: ... 20 min) and resuspended in M9 minimal media comprising 3 g/L 15N ammonium chloride (Cambridge Isotope Laboratories, NLM-467-25) with 10 g/L glucose or 10 g/L 13C glucose (Cambridge Isotope Laboratories ...
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Conformational dynamics at microsecond timescale in the RNA-binding regions of dsRNA-binding domainsbioRxiv - Biophysics 2019Quote: ... the protein was prepared by growing the cells in the M9 media containing 15NH4Cl (Cambridge Isotope Laboratories) as a sole source of nitrogen.
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bioRxiv - Molecular Biology 2021Quote: ... and proteins were produced in the M9 media supplemented with 2 g/L [15N]NH4Cl (Cambridge Isotope Laboratories) and 3 g/L d-[13C6]glucose (Cambridge Isotope Laboratories) ...
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bioRxiv - Developmental Biology 2021Quote: ... Islets were then incubated on a rotator plate at 95 RPM for 90 minutes at 37°C and 5% CO2 before being transferred to Eppendorf tubes and the basal KRB exchanged for a 0.9 ml volume of KRB containing either 3 mM (low) or 17 mM (high) [U-13C6] glucose (Cambridge Isotope Laboratories, CLM 1396). Islets were then incubated for 1 hour at 37°C and 5% CO2 ...
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bioRxiv - Biochemistry 2022Quote: ... MMA quantification was performed using 3 uL of plasma that was spiked in with [U-13C]-MMA (Cambridge Isotope Laboratories, cat# CLM-9426-PK) as previously described (31 ...
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bioRxiv - Molecular Biology 2020Quote: ... protein was expressed in BL21/DE3 Escherichia coli cells using M9 minimal media supplemented with 15NH4Cl (Cambridge Isotope Laboratories) induced at an OD600 of 0.6 with 0.5 mM IPTG for 16 hours at 18°C ...
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bioRxiv - Plant Biology 2023Quote: ... and 1 g/L KNO3 or 1 g/L K15NO3 (Cambridge Isotope Laboratories), pH 5.8 ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1 g/L KNO3 or 1 g/L K15NO3 (Cambridge Isotope Laboratories), pH 5.8 ...
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bioRxiv - Biophysics 2023Quote: ... 15N-labeled and 15N-13C labeled (as required) TRBP2-dsRBD2 protein was prepared using 15NH4Cl and 13C-glucose (Cambridge Isotope Laboratories) as a sole source of nitrogen and carbon ...
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bioRxiv - Biophysics 2024Quote: ... to obtain the 15N uniformly single labelled protein or in a media containing 15NH4Cl (0.1% w/v) and 13C-labelled glucose (0.4% w/v) (Cambridge isotope Laboratories, Inc.) for 15N and 13C uniformly double labelled protein ...
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bioRxiv - Biophysics 2023Quote: ... Uniformly-labeled 13C 15N Sup35NM samples were prepared by growing BL21(DES)-Rosetta Escherichia coli in the presence of M9 media with 2 g L-1 D-glucose 1H,13C6 and 1 g L-1 of 15N ammonium chloride (Cambridge Isotope Labs, Cambridge, MA). Purified ...
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bioRxiv - Biophysics 2019Quote: ... and uniformly 15N- or 15N,13C-labeled proteins were purified from cells grown in M9 minimal media with 15N ammonium chloride and/or 13C D-glucose (Cambridge Isotope Labs) as the sole nitrogen and/or carbon source ...
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bioRxiv - Biochemistry 2021Quote: ... then 10% of bead volume was lysed with 1% Triton buffer for protein extracts and the remaining 90% was extracted in 80% methanol containing heavy labeled amino acid standards (Cambridge Isotope Labs) on a rotator shaker for 10□min at 4□°C.
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bioRxiv - Biochemistry 2022Quote: ... and sortase A-compatible proteins were either expressed in Terrific Broth (TB) media or M9 media supplemented with 13C-glucose and/or 15NH4Cl (Cambridge Isotope Labs, Inc.) followed by protein induction with 0.5 mM isopropyl ß-D-1-thiogalactopyranoside (Gold Biotechnology ...
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bioRxiv - Biophysics 2024Quote: ... the cells were transferred to fresh LB medium for the overexpression of the unlabeled protein or to M9 minimal medium supplied with 15NH4Cl (Cambridge Isotope Laboratories Inc.) as the only nitrogen source for the labeled protein overexpression ...
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bioRxiv - Biochemistry 2021Quote: ... cells while expression was carried out in LB media for non-labeled protein and M9 minimal media containing 15NH4Cl (Cambridge Isotope Laboratories, Tewksbury, MA) for 15N ...
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bioRxiv - Physiology 2020Quote: ... constant infusion (0.0085 mg•kg-1•min-1) of [ring-13C6]-L-phenylalanine (Cambridge Isotope Laboratories, Tewksbury, MA) from 1000 to the end of the protocol ...
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bioRxiv - Biochemistry 2019Quote: ... with 1 g 15NH4Cl (Cambridge Isotope Laboratories) as the sole nitrogen source ...
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bioRxiv - Bioengineering 2022Quote: ... 100 % 1-13C glucose (Cambridge Isotope Laboratories), or unlabelled glucose ...
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bioRxiv - Biochemistry 2022Quote: ... 1 g/L 15NH4Cl (Cambridge Isotope Laboratories) and 50 μg/mL kanamycin ...
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bioRxiv - Cell Biology 2022Quote: ... Ultra pure bovine serum albumin (Cell Signalling Technology 9998) was used as a protein source and iPSDM were incubated with media containing 13C-labeled glucose (Cambridge Isotope Laboratories CLM-1396-5) and the described treatments for 1h before continuing with downstream sample processing ...
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bioRxiv - Cancer Biology 2020Quote: ... Heavy lysine (1G: CLM-265-H-1) and arginine (1G: CNLM-291-H-1) were purchased from Cambridge Isotope Laboratories (CIL ...