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Citations for Cambridge Isotope Labs :
101 - 150 of 247 citations for L Asparagine H2O 13C4 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... H2O/ACN/MeOH supplemented with stable isotopic labeled amino acids solution at 1 nmol/mL (MSK-A2-1.2, Cambridge Isotope Laboratories, USA).
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bioRxiv - Biochemistry 2021Quote: ... The labeled M9 minimal medium had the same composition except for the replacement of NH4Cl and glucose by [15N]NH4Cl and uniformly labeled [13C]glucose at the same concentrations (99% labeling for both compounds; Cambridge Isotope Laboratories). The growth media were sterilized by filtration (Millex-LG 0.2 µm ...
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bioRxiv - Microbiology 2021Quote: The ability of Thaumarchaeota to oxidize DON during growth on NH3 was assessed using 15N isotope tracers (98-99% 15N, Cambridge Isotope Laboratories). Axenic cultures were grown on SCM as described above except using 100 µM ammonium chloride and 20 µM pyruvate ...
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bioRxiv - Microbiology 2024Quote: ... 100 µL of each strain was transferred from the unlabeled glucose M9 media to 5 mL of M9 media with 50% 13C glucose (99% 13C, Cambridge Isotope Laboratory) and 50% natural abundance glucose and allowed to grow overnight at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... or L-Arginine U-13C6 and L-Lysine 4,4,5,5-2H4 (HMI11-R6K4) (Cambridge Isotope Laboratories) were added at 120 uM and 240 uM respectively ...
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bioRxiv - Cancer Biology 2022Quote: ... the cell pellet was resuspended into 1 mL of ice-cold 2:2:1 MeOH: ACN: H2O (v/v/v) containing 10 μM stable isotope-labeled canonical amino acid mix (Cambridge Isotope Laboratories, Inc) as internal standards ...
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bioRxiv - Biochemistry 2021Quote: ... 13C-1 labelled polyM was produced from fermentation of the same strain using 13C-1 (99 % 13C) D-fructose (Cambridge Isotope Laboratories, USA). This substrate was further subjected to acid hydrolysis as described elsewhere (39 ...
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bioRxiv - Plant Biology 2021Quote: ... In darkness the sample was purged with N2 to minimise background 16O2 before a bubble of 18O2 (99% Cambridge Isotope Laboratories Inc, UK) was loaded into the stirring liquid ...
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bioRxiv - Plant Biology 2022Quote: ... six leaf discs from the same leaf were transferred in a well of a 6-well microplate filled with 4 mL/well of a buffer containing 10 mM MES-KOH (pH 6.5) and 10 mM U-13C-pyruvate (13C isotopic purity of 99%; sodium salt from Cambridge Isotope Laboratories (Eurisotop, France)) ...
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bioRxiv - Plant Biology 2024Quote: ... A solution containing 15N was created by combining 15NKNO3 with a 99 atomic percent (15N) purity (Cambridge Isotope Laboratories, Inc., Andover, MA, USA) and unlabelled KNO3 (Merck ...
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bioRxiv - Molecular Biology 2019Quote: ... or L-arginine (13C6-15N4) and L-lysine (13C6-15N2) (heavy SILAC label) (Cambridge Isotope Laboratories).
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bioRxiv - Cancer Biology 2020Quote: ... or L-Arginine-13C6,15N4 (Cambridge Isotope), 200mg/L Proline (Sigma) ...
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bioRxiv - Cancer Biology 2020Quote: ... or L-Lysine-13C6,15N2 (Cambridge Isotope), 40mg/L Arginine (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... 13C5-L-ornithine (Cambridge Isotope Laboratories) was used as a control at a final concentration of 100 µM ...
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bioRxiv - Biophysics 2023Quote: ... The exo-PS accumulated on the 10 kDa membrane surface as a clear gel and was collected and dialyzed extensively against MilliQ-grade H2O or D2O (Cambridge Isotope Labs, 99.9% D). The H2O solutions provide scattering profiles at an additional contrast ...
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bioRxiv - Cell Biology 2023Quote: ... Taurine concentrations were then measured via LC-MS and analyzed the area of the exact mass for the corresponding ions of the targets as well as the intracellular labeled isotopic taurine levels (13C2, 99%; 15N, 98%, Cambridge isotope #CNLM-10253-PK).
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bioRxiv - Biophysics 2021Quote: ... 2 g/l of U-[2H,13C] D-glucose and 1 g/l 15NH4Cl (Cambridge Isotope Laboratories). For production of U-[2H ...
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bioRxiv - Systems Biology 2020Quote: ... or using ‘heavy’ (containing 20 mg/l L-arginine-13C-HCl (CLM-2265-H, Cambridge Isotope Labs) and 20 mg/l L-lysine-13C-HCl [CLM-2247-H ...
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bioRxiv - Biochemistry 2019Quote: ... with 10 g/L glucose or 10 g/L 13C glucose (Cambridge Isotope Laboratories, CLM-1396-25). Expression was induced using 1 mM isopropylthio-β-D-galactoside (IPTG ...
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bioRxiv - Cell Biology 2019Quote: ... and 0.4 mM isotopically-labelled L-arginine and 0.8 mM isotopically-labelled L-lysine (Cambridge Isotope Laboratory) (“Heavy” media ...
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bioRxiv - Cell Biology 2023Quote: ... R0) or 0.80 mM [U-13C6,15N2] L-lysine and 0.40 mM [U-13C6] L-arginine (Cambridge Isotope Laboratories ...
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bioRxiv - Biochemistry 2023Quote: ... media without L-leucine was supplemented with U-[13C]-L-leucine (Cambridge Isotope Laboratories, CLM-2262-H) at formulation concentration and was used in the place of chemically identical regular unlabeled media ...
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bioRxiv - Cell Biology 2019Quote: HEK293WT cells were labelled to >95% incorporation of 12C6,15N2 L-Lysine and 13C6,15N4 L-Arginine (Cambridge Isotope Laboratories) in SILAC DMEM media lacking L-Lysine and L-Arginine (Thermo Fischer) ...
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bioRxiv - Molecular Biology 2021Quote: ... [13C6][15N4] L-arginine (Arg-10) and [13C6][15N2] L-lysine (Lys-8) were added (Cambridge Isotope Laboratories). Both forward (heavy CNOT1 siRNA/medium-heavy control siRNA ...
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bioRxiv - Neuroscience 2023Quote: ... and heavy isotopes (4, 4, 5, 5-D4 L-lysine and 13C6 L-arginine, Cambridge Isotope Laboratories (CIL)) for the K4/R6 population ...
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bioRxiv - Microbiology 2019Quote: ... Except for the negative controls, either 13C-labelled methane (99 atom%; Isotec Inc., Matheson Trigas Products Division) or 13C-labelled bicarbonate (Cambridge Isotope Laboratories Inc., Tewksbury, USA) was used in the batch incubations ...
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bioRxiv - Plant Biology 2023Quote: ... we exchanged the centrifuge tube with one containing a 10 mM solution of either 13C1- or 13C2-pyruvate (99 atom % 13C, Cambridge Isotope Laboratories, Andover, MA, USA). We used the mass decline of the centrifuge tube over time to calculate the label uptake ...
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bioRxiv - Cancer Biology 2020Quote: ... 2mM L-glutamine-13C5 (Cambridge Isotope Laboratories) and 1.1mM L-arginine hydrochloride (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... and 1g/l 15NH4Cl (Cambridge Isotope Laboratories) and 100μg/l of carbenicillin ...
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bioRxiv - Cell Biology 2022Quote: ... and L-Valine-d8 (Cambridge Isotope Laboratories) were added to the supernatants ...
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bioRxiv - Evolutionary Biology 2020Quote: ... (2) D4 L-Lysine (Cambridge Isotope Laboratories) for “medium” (M ...
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bioRxiv - Microbiology 2023Quote: ... L-valine-13C5,15N was from Cambridge Isotope Laboratories ...
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bioRxiv - Biochemistry 2022Quote: ... 1 g/L 15NH4Cl (Cambridge Isotope Laboratories) and 50 μg/mL kanamycin ...
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bioRxiv - Cell Biology 2020Quote: ... L amino acids were substituted for the same concentrations of intermediate isotope–labeled (M) amino acids 4.4.5.5-D4 L-Lys:2HCl and 13C6 L-Arg (Cambridge Isotope Laboratories). All media were changed every other day for 8 to 10 days ...
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bioRxiv - Molecular Biology 2021Quote: ... a supplement of [13C6] L-arginine (Arg-6) and [2H4] L-lysine (Lys-4) was added (Cambridge Isotope Laboratories). For the heavy isotope containing medium ...
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bioRxiv - Biochemistry 2020Quote: ... This media was supplemented L-Arginine-13C 15N (R10) and L-Lysine-13C 15N (K8) (Cambridge Isotope Laboratories, Inc.) but not with FCS to prevent incorporation of 12C-Lysine or Arginine.
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bioRxiv - Immunology 2021Quote: ... and either L-Lysine-2HCl 13C6 15N2 and L-Arginine-HCl 13C6 15N4 (‘Heavy’ amino acids) or L-Lysine-2HCl 4,4,5,5-D4 and L-Arginine-HCl 13C6 (‘Medium’ amino acids) (all Cambridge Isotope Laboratories). Wt cells were grown in ‘Medium’ SILAC media and ifitm3-/- were grown in ‘Heavy’ SILAC media ...
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bioRxiv - Cell Biology 2022Quote: ... The second plate was cultured in medium SILAC media with the same composition as above except Arg0 and Lys0 were replaced by L-Arginine [13C6] HCl (Arg-6) and L-ysine-4,4,5,5-d4 (Lys-4) (Cambridge Isotope Laboratories). The third plate was cultured in heavy SILAC media with the same composition except arginine and lysine were replaced by L-Arginine [13C6,D7,15N4]HCl (Arg-17 ...
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bioRxiv - Microbiology 2021Quote: ... The cosms were surface-irrigated at 7 am with 60 ml of either 19.85 mM 15NH4Cl (99% 15N, Cambridge Isotope Laboratories, Inc., MA, US, pH 5.47) or a 19.98 mM 15KNO3 (99% 15N ...
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bioRxiv - Microbiology 2022Quote: ... DNA with different levels of 13C enrichment was produced by varying the ratio of unlabeled glucose to uniformly-labeled 13C6-D-glucose (Cambridge Isotope Laboratories; CLM-1396; 99 atom %), e.g ...
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bioRxiv - Cell Biology 2019Quote: ... 1% (v/v) penicillin/streptomycin and either unlabelled Arg and Lys or L-[13C6,15N4] Arg and L-[13C6,15N2] Lys (Cambridge Isotope Laboratories) at concentrations of 42 μg/ml (Arg ...
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bioRxiv - Microbiology 2021Quote: 13C-labeled cystine (1,1’-13C2-L-cystine) was prepared from 1-13C-L-cysteine (Cambridge Isotope Labs, CLM-3852-0.5) via a modification of the procedure of Hill ...
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bioRxiv - Cancer Biology 2019Quote: ... The samples were then dissolved in a mixture containing phosphate buffer (520 μL, 0.135 mol/L), D2O (50 μL) and TSP (30 μL, 5.8 mmol/L) (Cambridge Isotope Laboratories) as internal standard and were analyzed by NMR ...
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bioRxiv - Neuroscience 2020Quote: Primary hippocampal neurons were prepared from E18-19 rat brains and grown on poly-D-lysine coated 15 cm plates at a density of 3.5 million cells/ 15 cm plate in DMEM media without l-arginine or l-lysine (Cambridge Isotope Laboratories ...
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bioRxiv - Biochemistry 2021Quote: ... and 0.798 mM L-(13C6,15N2)-lysine (Lys”8”)] or “medium” amino acids [0.398 mM L-(15N4)-arginine (Arg”4”) and 0.798 mM L-(D4)-lysine (Lys”4”)] (Cambridge Isotope Laboratories), and incubated for 2 hr as previously described elsewhere (Imami et al. ...
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bioRxiv - Cell Biology 2022Quote: ... The third plate was cultured in heavy SILAC media with the same composition except arginine and lysine were replaced by L-Arginine [13C6,D7,15N4]HCl (Arg-17) and L-Lysine [13C6,15N2]HCl (Lys-8) (Cambridge Isotope Laboratories). Cells were passaged and expanded in the corresponding SILAC media for 10 doublings.
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bioRxiv - Systems Biology 2020Quote: ... heavy [13C6/15N2] L-lysine (Cambridge Isotope Labs) was added to a final concentration of 30 mg/l ...
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bioRxiv - Biochemistry 2020Quote: ... L-lysine-d4 (Cambridge Isotope Laboratories, DLM-2640), 112.5 μM ...
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bioRxiv - Cell Biology 2020Quote: ... or heavy L-arginine (R10, Cambridge Isotope Laboratories). SILAC labeled cells were counted after harvesting ...
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bioRxiv - Cell Biology 2020Quote: ... or heavy L-lysine (K8, Cambridge Isotope Laboratories) and 84 mg/ml light (R0 ...