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Citations for Cambridge Isotope Labs :
51 - 98 of 98 citations for Ion Exchange Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... M1C with uniform 13C and 15N isotope labels was expressed in 1L M9 minimal media containing 1.8 g 15NH4Cl and 2.0 g 13C-glucose (Cambridge Isotope Laboratories). Cells were grown at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... the experiment was additionally repeated in media made from 18O enriched water (OLM-240-10-1, Cambridge Isotope Laboratories, Inc.) at a final δ18Owater of approximately +100‰.
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bioRxiv - Immunology 2023Quote: ... On the final day all media conditions were replaced with 5 mM [U-13C]-fructose (Cambridge Isotope Laboratories, CLM-1553). Metabolites were extracted using 80% methanol 24 hours later ...
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bioRxiv - Cancer Biology 2023Quote: Isotopically enriched HHR WT/DelEx4 samples were grown in M9 minimal media containing 15NH4Cl and 13C glucose (Cambridge Isotope Labs) as the sole source of nitrogen and carbon respectively ...
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bioRxiv - Biophysics 2024Quote: ... 1 L cultures of autoinduction media (Cold Spring Harbor Protocols) were used with 15N labeled ammonium chloride (Cambridge Isotope Laboratories). The final antibiotic concentration was increased to 0.1 mg/mL of kanamycin to reduce plasmid loss and poor yields ...
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bioRxiv - Biochemistry 2022Quote: ... Cell pellets were resuspended in 250 mL of isotopically labelled minimal media M9 salt solution supplemented with 13C D-glucose 4.0 g/L and 15NH4Cl 1.0 g/L (Cambridge Isotope Laboratories, Inc.), then incubated to allow the recovery of growth and clearance of unlabeled metabolites ...
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bioRxiv - Molecular Biology 2020Quote: ... cultures were first grown at 37°C in 1 L of LB media until an OD600 of 0.6 before the cells were pelleted and resuspended in 0.5 L M9 minimal media prepared in D2O supplemented with 13C-D-glucose and 15NH4Cl (Cambridge Isotope Laboratories). After 1 hour recovery at 37°C ...
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bioRxiv - Plant Biology 2019Quote: ... Ca(NO3)2 × 4H2O in solid and liquid PpNO3 media was replaced by Ca(15NO3)2 × 4H2O (Cambridge Isotope Laboratories, England). To obtain fully labelled protonema tissue the protonema cultures were weekly sub-cultivated on fresh 15N labelled solid PpNO3 media for at least 4 months ...
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bioRxiv - Biochemistry 2019Quote: ... 20 min) and resuspended in M9 minimal media comprising 3 g/L 15N ammonium chloride (Cambridge Isotope Laboratories, NLM-467-25) with 10 g/L glucose or 10 g/L 13C glucose (Cambridge Isotope Laboratories ...
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bioRxiv - Biophysics 2021Quote: ... and the cell pellet was resuspended in 0.5L of M9 minimal media containing 15NH2Cl with either 13C-glucose or 5% 13C-glucose (Cambridge Isotope Laboratories). Incubation continued for about 1hr at 35 C when expression was induced with 1 mM IPTG ...
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bioRxiv - Cancer Biology 2021Quote: ... PaTu-8902 and MIAPaCa-2 iDox-shGOT2.1 cells were cultured in media containing 1 mM unlabelled glucose and 1 mM U13C-Pyruvate (Cambridge Isotope Laboratories) for 16 hours.
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bioRxiv - Microbiology 2023Quote: When the protein samples were prepared for NMR analysis the bacteria were cultured in M9 media containing D-[13C] glucose (U-13C6, 99%, CLM-1396-1, Cambridge Isotope) and 15NH4Cl (15NH4 Chloride ...
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bioRxiv - Cancer Biology 2023Quote: ... no-glucose and no-glutamine media was supplemented with 5 mM of 13C6 D-Glucose (Cambridge Isotope Laboratories, Inc., CLM-1396) and 4 mM unlabeled glutamine ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were seeded and treated as above and incubated in media in which glutamine was replaced with [U-13C]-glutamine (99% enrichment; Cambridge Isotope Laboratories ...
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bioRxiv - Biophysics 2023Quote: ... except that yeast cells were grown in SD-CSM media made with 2% (w/v) protonated 13C-depleted glucose (99.9% 12C, Cambridge Isotope Labs) as the carbon source ...
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bioRxiv - Cancer Biology 2023Quote: U-[13C]-Glc labelling media consisted of DMEM supplemented with 10 mM D-glucose (U-13C6; CLM-1396; Cambridge Isotope Laboratories), 2 mM glutamine ...
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bioRxiv - Cancer Biology 2023Quote: ... U-[13C]-Q labelling media consisted of DMEM supplemented with 2 mM L-glutamine (U-13C5; CLM-1822; Cambridge Isotope Laboratories), 10 mM glucose ...
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bioRxiv - Biophysics 2024Quote: ... to obtain the 15N uniformly single labelled protein or in a media containing 15NH4Cl (0.1% w/v) and 13C-labelled glucose (0.4% w/v) (Cambridge isotope Laboratories, Inc.) for 15N and 13C uniformly double labelled protein ...
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bioRxiv - Molecular Biology 2021Quote: ... expression was carried out in M9 minimal media supplemented with 3 g/L (13C6, 99%)-D-glucose and/or 1 g/l (15N, 99%)-NH4Cl (Cambridge Isotope Laboratories). To generate isotopically labeled rad6-A126T ...
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bioRxiv - Cancer Biology 2021Quote: ... CAFs seeded were seeded in 6 well plates at 2×105 cells/well and incubated for 72 hours in growth media containing U13C-Glucose (Cambridge Isotope Laboratories).
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bioRxiv - Cancer Biology 2022Quote: ... and re-plated for 12 hours in glutamine free media that was supplemented with either 2 mM 15N-glutamine (Cambridge isotope laboratories) or 2 mM GlutaMAX (Gibco Inc. ...
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bioRxiv - Biophysics 2022Quote: ... the cells were grown in M9 media made with D2O (99.8%, Sigma-Aldrich, St. Louis, MO/Cambridge Isotope Laboratories, Inc., Tewksbury, MA), 1 g/L 15NH4Cl (Sigma-Aldrich ...
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bioRxiv - Biophysics 2019Quote: ... and uniformly 15N- or 15N,13C-labeled proteins were purified from cells grown in M9 minimal media with 15N ammonium chloride and/or 13C D-glucose (Cambridge Isotope Labs) as the sole nitrogen and/or carbon source ...
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bioRxiv - Biophysics 2020Quote: ... Cells were pelleted and transferred to M9 media containing 1 g of 15N-ammonium chloride and 2 g of 13C uniformly label glucose (Cambridge Isotope Laboratories). After transfer ...
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bioRxiv - Cancer Biology 2022Quote: ... 3×106 cells/well were seeded in a 6-well plate and cultured in 2 mL assay media supplemented with 10% dialyzed FBS (Wisent) and either 25 mM U-[13C]-glucose (Cambridge Isotope Laboratories) and 4 mM glutamine (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... 200μL of media was added to a 2mL eppendorf tube followed by 40μL of 1mM 13C2-D3 sodium acetate (Cambridge Isotope #CDLM-3457) as internal standard ...
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bioRxiv - Biochemistry 2023Quote: ... 15N-labeled protein samples were grown in 1L minimal media containing 3 g/L 15N ammonium chloride (Cambridge Isotope Laboratories, Tweksbury, MA) and unlabeled protein samples were grown in 1L super broth media ...
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bioRxiv - Physiology 2024Quote: ... the cells were placed in 10 cm plates with media where the normal glucose was replaced with 13C6-L-glucose (Cambridge Isotope Laboratories). Cells were allowed to incubate in the 13C6-L-glucose prior to the in vitro ischemic-reperfusion injury (hypoxia/reoxygenation ...
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bioRxiv - Cancer Biology 2023Quote: ... Plates were then washed with PBS then switched to labeling media containing either 1 mM 13C5-glutamine (CLM-1822-H-PK, Cambridge Isotope Laboratories) or 5 mM 13C1,2-glucose (CLM-504-PK ...
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bioRxiv - Immunology 2024Quote: ... the media was changed to DMEM media containing 1% dialyzed FBS or 1% dialyzed FBS supplemented with 2mM [U-13C8]-octanoate (Cambridge Isotope Laboratories). After 24h ...
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bioRxiv - Cancer Biology 2024Quote: ... the media was removed and replaced with glutamine-free media supplemented with 10% dFBS and 4 mmol/L U-13C5-L-glutamine (CLM-1822-H, Cambridge Isotope Laboratories) for 2 hours prior to harvest (n = 3 per condition) ...
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bioRxiv - Cell Biology 2024Quote: ... 13C6-Glucose or 13C3-pyruvate were added for either the full 48 hours or in the last 8 hours accompanied by a media change (Cambridge Isotope Laboratories). Cells were then washed with ice-cold PBS three times and metabolites extracted using an extraction solvent consisting of 50% methanol ...
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bioRxiv - Cancer Biology 2020Quote: ... 10μL of media was mixed with 10μL of a mixture of isotopically labeled metabolites and amino acids of known concentrations (Cambridge Isotope Laboratories, Tewksbury, MA) and extracted with 600μL ice-cold HPLC grade methanol ...
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bioRxiv - Molecular Biology 2020Quote: ... Unlabeled samples were prepared using Luria Broth (LB) (Melford) and minimal media M9 with 15NH4Cl and/or D- [13C] glucose (Cambridge Isotope Laboratories, Inc) were used to prepare the labeled samples (25) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... using a pET-46 tobacco etch virus (TEV) protease-cleavable N-terminal hexahistidine tag fusion protein in M9 media supplemented with 15NH4Cl (Cambridge Isotope Labs, Inc.). Nurr1 LBD was eluted against a 500 mM imidazole gradient through a Ni-NTA column ...
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bioRxiv - Plant Biology 2022Quote: ... or 15N media (½ strength MS nutrient without nitrogen, 15NH 15NO [0.5 g/L, Cambridge Isotope Laboratory], K15NO3 [0.5 g/L, Cambridge Isotope Laboratory], pH 5.7) for 10 days ...
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bioRxiv - Cancer Biology 2023Quote: ... Alternatively post 48 hr LNCaP monoclonal CRISPR KOs were washed with PBS and media was replaced with DMEM (Dulbecco’s Modified Eagle’s Medium) medium containing 10 mM [U-13C]-glucose (Cambridge Isotope laboratories: #CLM-1396-10), 2 mM unlabeled glutamine and 10% dialyzed FBS (in triplicate) ...
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bioRxiv - Cancer Biology 2023Quote: ... organoid cultures were washed with PBS and media was replaced with DMEM (Dulbecco’s Modified Eagle’s Medium) medium containing 15 mM [U-13C]-glucose (Cambridge Isotope laboratories: #CLM-1396-10), 2 mM unlabeled glutamine ...
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bioRxiv - Biochemistry 2023Quote: Uniformly 15N labeled BTK samples were produced as described earlier by growth in modified M9 minimal media containing 15N ammonium chloride (1g/L, Cambridge Isotope Laboratories, Inc.) as the sole source of nitrogen [39] ...
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bioRxiv - Biophysics 2024Quote: ... The cells were used to inoculate 2L of H2O-based M9 media supplemented with 3 g L-1 of [U-13C6] d-glucose (Cambridge Isotope Laboratories, CIL) and 2 g L-1 of [15N] ammonium chloride (Cambridge Isotope Laboratories ...
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bioRxiv - Cell Biology 2021Quote: ... and Y858 (as Y859, but cdc55Δ ) cells were grown in free-methionine minimum media containing 13C6-lysine and -arginine (heavy, Cambridge Isotope Laboratories Inc., US) or unmodified arginine and lysine (light) ...
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bioRxiv - Biophysics 2023Quote: ... Uniformly-labeled 13C 15N Sup35NM samples were prepared by growing BL21(DES)-Rosetta Escherichia coli in the presence of M9 media with 2 g L-1 D-glucose 1H,13C6 and 1 g L-1 of 15N ammonium chloride (Cambridge Isotope Labs, Cambridge, MA). Purified ...
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bioRxiv - Cell Biology 2022Quote: ... Ultra pure bovine serum albumin (Cell Signalling Technology 9998) was used as a protein source and iPSDM were incubated with media containing 13C-labeled glucose (Cambridge Isotope Laboratories CLM-1396-5) and the described treatments for 1h before continuing with downstream sample processing ...
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bioRxiv - Biochemistry 2023Quote: ... uniformly 15N-labeled and 13C/15N-labeled Cd-IF1 were over-expressed in M9 media with 15NH4Cl and 15NH4Cl/13C-glucose (Cambridge Isotope Laboratories, Inc. Andover, MA) using the high-yield protein expression protocol [11] and purified as previously described [12] ...
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bioRxiv - Neuroscience 2023Quote: ... neurons were gently washed with PBS twice and switched into media containing the same components except for replacing light lysine with heavy stable isotope labeled (13C615N2) lysine (Cambridge Isotope Laboratories #CNLM-291-H-PK). For multiple time point experiments ...
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bioRxiv - Cancer Biology 2023Quote: ... Tracing of glutamine was performed by supplementing no-glucose and no-glutamine media with 4 mM 13C5 L-glutamine (Cambridge Isotope Laboratories, Inc., CLM-1822-H) and 5 mM unlabeled glucose ...
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bioRxiv - Cancer Biology 2019Quote: ... 15-20E + 6 cells in light SILAC media were treated with drug compound and cells in heavy SILAC media (L-Lysine-13C6,15N2, L-Arginine-13C6,15N4 (Cambridge Isotope, CNLM-291-H-1, CNLM-539-H-1) were treated with DMSO for 24 hr ...
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bioRxiv - Biophysics 2023Quote: ... They were then resuspended in 500 ml of M9 media supplemented with 100 mg of a desired 15N-labeled or 13C-labeled L-amino acid (Cambridge Isotope Labs, Inc. or Sigma-Aldrich, Inc.) as well as the remaining 19 unlabeled amino acids ...