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Citations for Cambridge Isotope Labs :
51 - 94 of 94 citations for Histone Lysine N Methyltransferase EZH2 EZH2 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... or U-13C6-N- acetylglucosamine (Cambridge Isotope Laboratories, CLM-1827) and incubated in a conical tube for 5 hours in humidified incubators at 37 °C and 5% CO2 prior to addition of excess ice-cold PBS ...
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bioRxiv - Molecular Biology 2019Quote: ... or L-arginine (13C6-15N4) and L-lysine (13C6-15N2) (heavy SILAC label) (Cambridge Isotope Laboratories).
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bioRxiv - Cancer Biology 2022Quote: ... 84 mg/l 13C615N4 L-arginine and 175 mg/l 13C615N2 L-lysine (Cambridge Isotope Laboratories) until they were fully labeled ...
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bioRxiv - Biochemistry 2020Quote: ... or ‘heavy’ 13C615N2-Lysine (Silantes; 211604102) and 13C615N4-Arginine (Cambridge Isotope Labs Inc; CNLM-539-H-1). Cells were cultured in SILAC ‘light’ or ‘heavy’ media for two weeks prior to harvesting by centrifugation (800g ...
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bioRxiv - Microbiology 2021Quote: Heavy medium (HM) was prepared by adding 146 mg/mL [13C6,15N2]L-Lysine-2HCl (Cambridge Isotope Laboratories) and 84 mg/mL [13C6,15N4]L-Arginine-HCl (Cambridge Isotope Laboratories ...
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bioRxiv - Cell Biology 2019Quote: ... and 0.4 mM isotopically-labelled L-arginine and 0.8 mM isotopically-labelled L-lysine (Cambridge Isotope Laboratory) (“Heavy” media ...
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bioRxiv - Cell Biology 2023Quote: ... R0) or 0.80 mM [U-13C6,15N2] L-lysine and 0.40 mM [U-13C6] L-arginine (Cambridge Isotope Laboratories ...
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bioRxiv - Cell Biology 2019Quote: HEK293WT cells were labelled to >95% incorporation of 12C6,15N2 L-Lysine and 13C6,15N4 L-Arginine (Cambridge Isotope Laboratories) in SILAC DMEM media lacking L-Lysine and L-Arginine (Thermo Fischer) ...
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bioRxiv - Molecular Biology 2021Quote: ... [13C6][15N4] L-arginine (Arg-10) and [13C6][15N2] L-lysine (Lys-8) were added (Cambridge Isotope Laboratories). Both forward (heavy CNOT1 siRNA/medium-heavy control siRNA ...
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bioRxiv - Neuroscience 2023Quote: ... and heavy isotopes (4, 4, 5, 5-D4 L-lysine and 13C6 L-arginine, Cambridge Isotope Laboratories (CIL)) for the K4/R6 population ...
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Domain-specific quantification of prion protein in cerebrospinal fluid by targeted mass spectrometrybioRxiv - Neuroscience 2019Quote: ... coli in 15N cell growth medium (Cambridge Isotope Laboratories CGM-1000-N) induced with 15N auto-induction medium (Millipore 71759-3) ...
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bioRxiv - Cancer Biology 2020Quote: ... Heavy lysine (1G: CLM-265-H-1) and arginine (1G: CNLM-291-H-1) were purchased from Cambridge Isotope Laboratories (CIL ...
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bioRxiv - Molecular Biology 2021Quote: ... a supplement of [13C6] L-arginine (Arg-6) and [2H4] L-lysine (Lys-4) was added (Cambridge Isotope Laboratories). For the heavy isotope containing medium ...
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bioRxiv - Biochemistry 2020Quote: ... This media was supplemented L-Arginine-13C 15N (R10) and L-Lysine-13C 15N (K8) (Cambridge Isotope Laboratories, Inc.) but not with FCS to prevent incorporation of 12C-Lysine or Arginine.
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bioRxiv - Immunology 2021Quote: ... and either L-Lysine-2HCl 13C6 15N2 and L-Arginine-HCl 13C6 15N4 (‘Heavy’ amino acids) or L-Lysine-2HCl 4,4,5,5-D4 and L-Arginine-HCl 13C6 (‘Medium’ amino acids) (all Cambridge Isotope Laboratories). Wt cells were grown in ‘Medium’ SILAC media and ifitm3-/- were grown in ‘Heavy’ SILAC media ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... 84 mg/l 13C615N4 L-arginine and 175 mg/l 13C615N2 L-lysine (Cambridge Isotope Laboratories, Inc., Tewksbury, MA). All the cell lines were cultured under standard conditions (37°C and 5% CO2 ...
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bioRxiv - Biochemistry 2019Quote: ... coli in 15N-enriched cell growth medium (Cambridge Isotope Laboratories CGM-1000-N) supplemented with Overnight Express Autoinduction Medium (Millipore-Sigma 71300-4) ...
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bioRxiv - Biochemistry 2023Quote: ... [2H4]N-palmitoyl-PE was synthesized using [2H4] palmitic acid (Cambridge Isotope Laboratories) and 1,2-dioleoyl-PE and N-oleoyl-PE was synthesized using 1,2-dihexanoyl-PE and oleoyl chloride (MilliporeSigma ...
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bioRxiv - Neuroscience 2020Quote: Primary hippocampal neurons were prepared from E18-19 rat brains and grown on poly-D-lysine coated 15 cm plates at a density of 3.5 million cells/ 15 cm plate in DMEM media without l-arginine or l-lysine (Cambridge Isotope Laboratories ...
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bioRxiv - Cell Biology 2019Quote: ... and the heavy amino acids L-Arginine 13C6 15N4 (147.5 mg/l) and L-Lysine 13C6 15N2 (91.25 mg/l; Cambridge Isotope Laboratories), or equal amounts of light arginine and lysine (Sigma) ...
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bioRxiv - Biochemistry 2021Quote: ... and 0.798 mM L-(13C6,15N2)-lysine (Lys”8”)] or “medium” amino acids [0.398 mM L-(15N4)-arginine (Arg”4”) and 0.798 mM L-(D4)-lysine (Lys”4”)] (Cambridge Isotope Laboratories), and incubated for 2 hr as previously described elsewhere (Imami et al. ...
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bioRxiv - Cell Biology 2022Quote: ... The third plate was cultured in heavy SILAC media with the same composition except arginine and lysine were replaced by L-Arginine [13C6,D7,15N4]HCl (Arg-17) and L-Lysine [13C6,15N2]HCl (Lys-8) (Cambridge Isotope Laboratories). Cells were passaged and expanded in the corresponding SILAC media for 10 doublings.
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bioRxiv - Systems Biology 2019Quote: ... for “heavy” (“H”)-labeled cells 13C615N4-L-arginine (89 mg/L) and 13C615N2-L-lysine (154 mg/L)(Cambridge Isotope Laboratories). Cells were split into each SILAC formulation and passaged six times to allow for complete incorporation of amino acid isotopologues.
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bioRxiv - Cancer Biology 2020Quote: ... 2mM were cultured with 2H4-lysine and 13C6-arginine (medium labelling) and 4mM were cultured with 15N2 13C6-lysine and 15N4 13C6-arginine (heavy labelling) (Cambridge Isotope Laboratory ...
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bioRxiv - Molecular Biology 2022Quote: ... medium (L-arginine:HCL [U13C6] and L-lysine:2HCL [4,4,5,5-D4], or heavy (L-arginine:HCL (13C6, 15N4) and L-lysine:2HCL (13C6, 15N2)) amino acid isotopes (Cambridge Isotope Laboratories Inc.).
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bioRxiv - Cell Biology 2020Quote: ... Two HeLa cultures were grown in SILAC in RPMI(-)Arg(-)Lys supplemented with 10% dialyzed FBS and either with 100 ng/ml of 13C Lysine plus 30 ng/ml of 13C Arginine (Cambridge isotope Laboratories) for heavy labelling ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% FBS and heavy isotope-labeled amino acids (L-Lysine 13C6, L-Arginine 13C6) (CLM-2247-H-1, CLM-2265-H-1, Cambridge Isotope Laboratories). DAC-treated cells grown in the regular medium were considered as SILAC light counterparts.
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bioRxiv - Biochemistry 2020Quote: ... and 0.798 mM L-(13C6,15N2)-lysine (Lys“8”)] or “medium” amino acids [0.398 mM L-(15N4)-arginine (Arg“4”) and 0.798 mM L-(D4)-lysine (Lys“4”)] (Cambridge Isotope Laboratories, Tewksbury, USA), and incubated for 2 h ...
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bioRxiv - Cell Biology 2021Quote: ... which were replaced with either unlabelled amino-acids (Lys0 and Arg0) or stable isotopic forms 13C6 15N2-lysine (Lys8) and 13C6 15N4-arginine (Arg10) (Cambridge Isotope Laboratories). The medium was supplemented with 10% dialyzed FBS using previous published methods (Golebiowski et al ...
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bioRxiv - Cancer Biology 2021Quote: ... whereas the medium for the ‘heavy’ labelled cells contained 84 mg/l 15N L-arginine and 175 mg/l C615N2 L-lysine (Cambridge Isotope Laboratories). Cells were regularly tested for mycoplasma and MCF10DCIS.com cells authenticated ...
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bioRxiv - Biochemistry 2022Quote: ... and 0.798 mM L-(13C6,15N2)-lysine (Lys”8”)] or “medium-heavy” amino acids [0.398 mM L-(13C6)-arginine (Arg”6”) and 0.798 mM L-(D4)-lysine (Lys”4”)] (Cambridge Isotope Laboratories, Tewksbury, USA). For chloramphenicol (CAP ...
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bioRxiv - Microbiology 2023Quote: ... or medium (84 mg L−1 13C6 L-arginine and 146 mg L−1 2H4 L-lysine) SILAC amino acids (Cambridge Isotope Laboratories). Medium SILAC labeled HeLa or HeLa S3 cells were used as spike-in control ...
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bioRxiv - Cell Biology 2023Quote: ... and either “Heavy” amino acids (50 μg/ml 13C6, 15N4 Arginine HCl and 100 μg/ml 13C6, 15N2 Lysine 2HCl; Cambridge Isotope Laboratories), or the equivalent “Light” amino acids ...
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bioRxiv - Cell Biology 2024Quote: ... 10 µL supernatant was added 40 µL solution of stable isotope-labeled internal standards (phenylalanine-D5, tyrosine-D4, valine-D8, methionine-D3, lysine-13C6-15N2, and arginine 13C6-15N4; Cambridge Isotope Laboratories) and 180 µL solvent (water with 0.2% heptafluorobutyric acid) ...
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bioRxiv - Neuroscience 2020Quote: F2 SILAM wide type female mice were purchased from Cambridge Isotope Laboratories and then crossed with regular C57BL6 wild type males by fed with stable isotope labeling using amino acids in cell culture (SILAC) L-lysine (13C6) feed (Cambridge Isotope Laboratories, Inc). After 7 days ...
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bioRxiv - Cell Biology 2021Quote: ... and Y858 (as Y859, but cdc55Δ ) cells were grown in free-methionine minimum media containing 13C6-lysine and -arginine (heavy, Cambridge Isotope Laboratories Inc., US) or unmodified arginine and lysine (light) ...
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bioRxiv - Cell Biology 2023Quote: ... L-LYSINE:2HCL (13C6, 99%;15N2, 99%) and stable isotope-labeled (heavy) L-ARGININE:HCL (13C6, 99%; 15N4, 99%) (Cambridge Isotope Labs, Inc. Andover, MA). Light SILAC medium was generated by adding unlabeled L-LYSINE:2HCL (final concentration 146.0 mg/mL ...
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bioRxiv - Biochemistry 2020Quote: ... 15N AIM media was composed of 1X BioExpress 15N cell growth media (Cambridge isotope labs CGM-1000-N) in EMD Millipore Overnight Express induction system (Sigma-Aldrich 71300-M ...
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bioRxiv - Cancer Biology 2019Quote: ... 15-20E + 6 cells in light SILAC media were treated with drug compound and cells in heavy SILAC media (L-Lysine-13C6,15N2, L-Arginine-13C6,15N4 (Cambridge Isotope, CNLM-291-H-1, CNLM-539-H-1) were treated with DMSO for 24 hr ...
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bioRxiv - Developmental Biology 2022Quote: ... and L-Lysine (0.2 mM) were replaced with the non-radioactive isotopic forms Arg-10 (13C6 H14 15N4O2; Cambridge Isotope Laboratories, cat. no. CNLM-539-H-PK) and Lys-8 (13C6 H14 15N2O2 ...
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bioRxiv - Immunology 2023Quote: 2×106 BMDCs were treated with 1mM uniformly labeled 13C lactate (L-LA*) (CLM-1579-N-0.1MG, Cambridge Isotope Laboratories), in the presence or absence of LPS (100ng/mL) ...
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Sex-specific fear acquisition following early life stress is linked to amygdala glutamate metabolismbioRxiv - Animal Behavior and Cognition 2024Quote: ... U-13C15N-labeled amino acids at a final concentration of 0.25 µM (prepared as 2.5 mM in 0.1 N HCl, Cambridge Isotope Laboratories, MSK-A2-1.2), citric acid d4 at 0.02 µg/mL (dissolved at 100 µg/mL in H2O ...
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bioRxiv - Microbiology 2021Quote: ... uniformly 15 N- and 13 C-labeled proteins were produced by growing cell cultures in M9 minimal medium that contained 1 g/l 15 N-ammonium chloride and 2 g/l 13 C-D-glucose (Cambridge Isotope Laboratories, Inc.) as the sources of nitrogen and carbon ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... using a pET-46 tobacco etch virus (TEV) protease-cleavable N-terminal hexahistidine tag fusion protein in M9 media supplemented with 15NH4Cl (Cambridge Isotope Labs, Inc.). Nurr1 LBD was eluted against a 500 mM imidazole gradient through a Ni-NTA column ...