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Citations for Cambridge Isotope Labs :
101 - 144 of 144 citations for Herpes Simplex Virus 1 Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... 1 L cultures of autoinduction media (Cold Spring Harbor Protocols) were used with 15N labeled ammonium chloride (Cambridge Isotope Laboratories). The final antibiotic concentration was increased to 0.1 mg/mL of kanamycin to reduce plasmid loss and poor yields ...
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bioRxiv - Biophysics 2021Quote: ... The cells were collected by centrifugation and resuspended in M9 medium (1 L, 100% D2O) supplemented with 15NH4Cl (Cambridge Isotope Laboratories) and 12C6H12O6 as the sole nitrogen and carbon sources ...
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bioRxiv - Microbiology 2021Quote: ... each of which was 25% labeled with 13C (i.e. mixed 1:4 with 25% H13CO3, 13C12H6O12, or CH313CO2, respectively; Cambridge Isotope Laboratories). All saltwater media was adapted from the Halohandbook and contained NaCl ...
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bioRxiv - Biochemistry 2021Quote: ... Extraction solution contained 80:20 methanol:water with 1:2500 dilution of a heavy amino acid mix standard (MSK-A2, Cambridge Isotope Laboratories); derivatization solution is described below ...
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bioRxiv - Cancer Biology 2021Quote: ... and polar metabolites extracted in 1 mL of cold 80% methanol containing internal standards (MSK-A2-1.2, Cambridge Isotope Laboratories, Inc.) by scraping the cells in each well on ice and collecting the content in 1.5 mL tubes ...
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bioRxiv - Biophysics 2022Quote: HDX samples of I552A SLO-1 were exchanged as a function of time by a 10-fold dilution into D2O (99% D; Cambridge Isotope) buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... Omental tumor biopsies from HGSOC patients were sectioned (0.5-1 mm) and incubated in DMEM supplemented with 10 mM [U-13C]-glucose (Cambridge Isotope Laboratories) and 2 mM glutamine ...
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bioRxiv - Cancer Biology 2023Quote: GMP clones were lysed in 100 µl ice-cold 80% methanol in water and polar metabolites were extracted using methanol-chloroform phase separation (1 ml methanol containing 2.5 µM of an internal standard (13C-, 15N-labeled amino acid mix; Cambridge Isotope Laboratories), 500 µl water ...
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bioRxiv - Microbiology 2023Quote: When the protein samples were prepared for NMR analysis the bacteria were cultured in M9 media containing D-[13C] glucose (U-13C6, 99%, CLM-1396-1, Cambridge Isotope) and 15NH4Cl (15NH4 Chloride ...
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bioRxiv - Cancer Biology 2023Quote: ... Two transitions were optimised using an authentic standard of glycocholic acid (glycine-1-13C, CLM-191-PK, Cambridge Isotope Laobratories, UK), from the negative precursor ion (m/z 465 ...
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bioRxiv - Biophysics 2024Quote: ... 4.0 gL-1 of D-glucose provided in specific isotopic ratios (12C, Merek, Germany; 13C, Cambridge Isotope Laboratories, USA; Table S1). 109 cells were harvested at late-exponential growth phase (Figure S3) ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were washed with PBS and fed 1 ml of culture medium supplemented with 2 mM [U-13C]glutamine (CLM-1822, Cambridge Isotope Laboratories) or 12.5 mM [1,2-13C]glucose (CLM-504-1 ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were washed with 0.9% NaCl twice and lysed with pre-chilled extraction buffer that was composed of 480 ul of MeOH mixed with 120 ul of H2O and 1 uM of pre-mixed Heavy Amino Acid mix (Cambridge Isotope Laboratories). Lysates were transferred to an Eppendorf tube and vortexed at 4 °C for 10 min before being centrifuged at 16,000 g at 4 °C for 10 min ...
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bioRxiv - Molecular Biology 2021Quote: ... expression was carried out in M9 minimal media supplemented with 3 g/L (13C6, 99%)-D-glucose and/or 1 g/l (15N, 99%)-NH4Cl (Cambridge Isotope Laboratories). To generate isotopically labeled rad6-A126T ...
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bioRxiv - Developmental Biology 2021Quote: ... /water (2:2:1) containing isotope-labeled internal standards ([U-13C6]glucose; [U-13C3]lactate; [U-13C5]glutamine; and [U-13C5]glutamate; Cambridge Isotope Laboratories) were spiked into media samples ...
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bioRxiv - Biochemistry 2022Quote: ... modified glucose-free DMEM (Hyclone) was adjusted to 25 mM glucose with either 1-13C-glucose or 6-13C-glucose (Cambridge Isotope Laboratories). Cells were reverse transfected as described above ...
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bioRxiv - Biophysics 2020Quote: ... harvested at an OD600nm of 3 and resuspended in 2 L of M9 minimal medium with isotopic enrichment (1 g/L of 15NH4Cl and/or 3 g/L of 13C6-glucose, Cambridge Isotope Laboratories). After 30-45 min ...
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bioRxiv - Cell Biology 2022Quote: ... Metabolites were extracted with 1 mL 80% MeOH pre-cooled to −80 °C containing 10 nmol [D8]-valine as an internal standard (Cambridge Isotope Labs). Insoluble material was removed by centrifugation at 21,000 ×g for 15 min at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... 15N-labeled SARS-CoV-2 nsp7 sample was expressed and purified by the same methods as previously described in our NMR assignment paper.20 The recombinant Escherichia coli Rossetta2 pLysS was first grown in a M9 minimal medium containing 1 g/L 15N4HCl (Cambridge Isotope Laboratories), 4 g/L 13C6-D-Glucose (Cambridge Isotope Laboratories ...
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bioRxiv - Biophysics 2020Quote: ... Cells were pelleted and transferred to M9 media containing 1 g of 15N-ammonium chloride and 2 g of 13C uniformly label glucose (Cambridge Isotope Laboratories). After transfer ...
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bioRxiv - Cancer Biology 2022Quote: ... H2O/ACN/MeOH supplemented with stable isotopic labeled amino acids solution at 1 nmol/mL (MSK-A2-1.2, Cambridge Isotope Laboratories, USA).
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bioRxiv - Cancer Biology 2023Quote: ... Plates were then washed with PBS then switched to labeling media containing either 1 mM 13C5-glutamine (CLM-1822-H-PK, Cambridge Isotope Laboratories) or 5 mM 13C1,2-glucose (CLM-504-PK ...
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bioRxiv - Cancer Biology 2021Quote: ... activity in cultured melanoma cells was quantitated by measuring the transfer of 15N from [α–15N]glutamine (NLM-1016-1, Cambridge Isotope Laboratories) to 15NH4+ as described (37 ...
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bioRxiv - Microbiology 2021Quote: ... uniformly 15 N- and 13 C-labeled proteins were produced by growing cell cultures in M9 minimal medium that contained 1 g/l 15 N-ammonium chloride and 2 g/l 13 C-D-glucose (Cambridge Isotope Laboratories, Inc.) as the sources of nitrogen and carbon ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell pellets were resuspended in 1 mL of 80% methanol solution containing 500 nM internal standards (Metabolomics Amino Acid Mix, Cambridge Isotope Laboratories Inc.). Samples were vortexed at 4 degrees ...
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bioRxiv - Microbiology 2021Quote: ... The concentrations ranged from 0.1 to 1000 ng.mL-1 for each congener and 0.1 to 100 ng/ml for surrogates (Cambridge Isotope Labs Inc. Part# EDF-5524) (Table S3) ...
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bioRxiv - Immunology 2020Quote: ... 25 μl of serum and 0.5 ml of Lipid Extraction Buffer containing 1 μg of D2-oleic acid (Cambridge isotope: DLM-689-0.1) and 1 μg of D14-palmitoleic acid (Cayman ...
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bioRxiv - Molecular Biology 2021Quote: ... For the 15N isotope labeling cells were cultivated at 37 °C in M9 minimal medium with 1 g/L 15NH4Cl (Cambridge Isotope Laboratories, Inc.).
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bioRxiv - Cancer Biology 2022Quote: ... the cell pellet was resuspended into 1 mL of ice-cold 2:2:1 MeOH: ACN: H2O (v/v/v) containing 10 μM stable isotope-labeled canonical amino acid mix (Cambridge Isotope Laboratories, Inc) as internal standards ...
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bioRxiv - Biophysics 2024Quote: ... The cells were used to inoculate 2L of H2O-based M9 media supplemented with 3 g L-1 of [U-13C6] d-glucose (Cambridge Isotope Laboratories, CIL) and 2 g L-1 of [15N] ammonium chloride (Cambridge Isotope Laboratories ...
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bioRxiv - Biophysics 2022Quote: ... then centrifuging and resuspending in M9 minimal medium containing 2 g/L unlabeled glucose and 1 g/L 15N-labeled ammonium acetate (Cambridge Isotope Labs, Tewksbury, MA) and induced with 1 mM IPTG ...
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bioRxiv - Biochemistry 2022Quote: ... a 100 mL LB starter culture treated with 100 μg/mL ampicillin was grown at 30 °C overnight and was used to inoculate 4× 1 L of M9 minimal medium containing 15NH4Cl and/or 13C6-glucose (Cambridge Isotope, Tewksbury, MA, USA). The cells were grown in this isotopically-enriched minimal media at 37 °C and shaken at 200 rpm until the OD600 reached 0.6-0.8 before a brief cold shock at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... 1 % P/S and isotope-labelled D3-15N-L-serine (1 mM unless indicated otherwise) and D4-L-alanine 2 mM (Cambridge Isotope Laboratories, MA, USA). Cells were grown further in the labelling media for 16 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... Dried samples were rehydrated with 2:1 methanol:water into which a mixture of isotopically labeled amino acids of known concentrations (Cambridge Isotope Laboratories, MSK-A2-1.2) was added ...
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bioRxiv - Synthetic Biology 2021Quote: ... a 80:20 mixture of 6-13C glucose:U-13C6 glucose or a 80:20 mixture of 1-13C glucose:U-13C6 glucose (Cambridge Isotope, MA and Omicron Biochemicals, IN). Cultures used as internal standards for metabolomic measurements were grown in two subsequent M9 minimal mediums cultures containing 100% U-13C6.
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bioRxiv - Cell Biology 2020Quote: ... 500 μl of each cell lysate sample was blown to dryness with N2 and reconstituted with 150 μL of an Acetonitrile/H2O (2:1) solution containing stable isotope-labeled internal standards: tyrosine-d2 and lactate-13C3 (Cambridge Isotope Lab, Tewksbury, MA). Centrifuged the cell lysate at 10,000 g for 20 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... with 1 mL of dry-ice cold 80% methanol/20% water solution containing 1.5 μM Cambridge AA mix (Cambridge Isotope Laboratories, MSK-A2-1.2). Immediately after ...
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bioRxiv - Cancer Biology 2023Quote: 5 μL of adult bovine serum or ftABSB was mixed with 45 μL of HPLC-grade methanol:water (80:20) including 500 nM 13C/15N labeled amino acids (Cambridge Isotope Laboratories, MSK-CAA-1). Samples were vortexed for 15 min at 4°C and insoluble material was sedimented by centrifugation at 16,000 x g for 10 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 μL of media was mixed with 45 μL of HPLC-grade methanol:water (80:20) including 500 nM 13C/15N labeled amino acids (Cambridge Isotope Laboratories, MSK-CAA-1). Samples were then vortexed for 15 min at 4°C ...
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bioRxiv - Biophysics 2024Quote: ... 15N isotope-labeled Ac-α-syn or α-syn were produced in M9 minimal medium with uniformly labeled 15NH4Cl (1 g/L, Cambridge Isotope Laboratories). Not isotope-labeled Ac-α-syn or α-syn were produced in LB medium ...
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bioRxiv - Microbiology 2024Quote: ... Pellets were reconstituted in 60 μl acetonitrile:ultrapure water (4:1, containing 13C6/15N-isoleucine as internal standard, 40 μM, Cambridge Isotope Laboratories, CNLM-561-H) and vortexed vigorously ...
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bioRxiv - Microbiology 2019Quote: ... Duplicate microcosms were fed with a 1 M solution of either 12C sodium butyrate or 13C-labeled sodium butyrate (>98% atom purity, Cambridge Isotope Laboratories, Tewksbury, MA, USA) to reach an initial butyrate concentration of 40 mM ...
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bioRxiv - Microbiology 2021Quote: ... The sample was spiked with 10 pg.g-1 of an isotopically labelled surrogate compound mixture containing 13C labelled surrogates of the 17 congeners analyzed (Cambridge Isotope Laboratories Inc. Part # EDF 5393). The samples were extracted with toluene at 200°C and 2000 psi for 10 min (Dionex ASE 350) ...
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bioRxiv - Biochemistry 2022Quote: Amino acids were quantified from 10 µL plasma precipitated with 180 µl ice-cold methanol containing 1 nmol of stable isotope labelled amino acids (Cambridge Isotope Laboratories, MSK-A2-1.2). Samples were incubated at -20°C for 30 min followed by centrifugation at 4°C (14,000g ...