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Citations for Cambridge Isotope Labs :
301 - 350 of 422 citations for Dl Serine 1 13C 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... and uniformly 15N- or 15N,13C-labeled proteins were purified from cells grown in M9 minimal media with 15N ammonium chloride and/or 13C D-glucose (Cambridge Isotope Labs) as the sole nitrogen and/or carbon source ...
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bioRxiv - Neuroscience 2023Quote: ... each rat was fed 1g of a pancake that contained 4 µl of [13C]-octanoic acid (Cambridge Isotope Laboratories, Inc, Tewksbury MA). After visually confirming the rats had eaten the entire pancake (in less than 5min or they were omitted from the study) ...
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bioRxiv - Cancer Biology 2022Quote: ... 3×106 cells/well were seeded in a 6-well plate and cultured in 2 mL assay media supplemented with 10% dialyzed FBS (Wisent) and either 25 mM U-[13C]-glucose (Cambridge Isotope Laboratories) and 4 mM glutamine (Sigma ...
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bioRxiv - Physiology 2023Quote: Metabolomics was performed on microglia by first washing microglia primary cell cultures with PBS and re-cultured in DMEM (lacking glucose) containing 10% dialyzed FBS and uniformly labeled [13C]-Glucose (Cambridge Isotope Laboratories). Microglia (2 x 106 per well in 6 well plates ...
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bioRxiv - Cancer Biology 2023Quote: ... Media was aspirated from cells and then washed rapidly in ice cold bank saline followed by addition of 500 mL ice-cold 80% methanol in water containing 500 nM each of 17 isotopically labeled 13C/15N amino acids (Cambridge Isotope Laboratories). Alternatively ...
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bioRxiv - Molecular Biology 2020Quote: ... Unlabeled samples were prepared using Luria Broth (LB) (Melford) and minimal media M9 with 15NH4Cl and/or D- [13C] glucose (Cambridge Isotope Laboratories, Inc) were used to prepare the labeled samples (25) ...
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bioRxiv - Biophysics 2020Quote: ... while 15N and 15N,13C samples for NMR studies were produced using minimal medium (M9) containing 0.1% 15NH4Cl (Cambridge Isotope Laboratories, Tewksbury, MA) or 0.1% 15NH4Cl and 0.3% U-13C-D-glucose (Cambridge Isotope Laboratories ...
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bioRxiv - Biochemistry 2022Quote: ... and sortase A-compatible proteins were either expressed in Terrific Broth (TB) media or M9 media supplemented with 13C-glucose and/or 15NH4Cl (Cambridge Isotope Labs, Inc.) followed by protein induction with 0.5 mM isopropyl ß-D-1-thiogalactopyranoside (Gold Biotechnology ...
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bioRxiv - Physiology 2023Quote: ... hearts were perfused for 20 minutes with a modified KH-buffer containing uniformly labelled glucose (5 mmol/L [U-13C]glucose, Cambridge Isotope Laboratories, Inc.) instead of standard glucose ...
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bioRxiv - Cancer Biology 2023Quote: ... Alternatively post 48 hr LNCaP monoclonal CRISPR KOs were washed with PBS and media was replaced with DMEM (Dulbecco’s Modified Eagle’s Medium) medium containing 10 mM [U-13C]-glucose (Cambridge Isotope laboratories: #CLM-1396-10), 2 mM unlabeled glutamine and 10% dialyzed FBS (in triplicate) ...
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bioRxiv - Cancer Biology 2023Quote: ... organoid cultures were washed with PBS and media was replaced with DMEM (Dulbecco’s Modified Eagle’s Medium) medium containing 15 mM [U-13C]-glucose (Cambridge Isotope laboratories: #CLM-1396-10), 2 mM unlabeled glutamine ...
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bioRxiv - Cancer Biology 2023Quote: ... The tissue powder was then weighed into pre-chilled tubes and extracted with methanol (containing 500 nM each of 17 isotopically labeled 13C/15N amino acids (Cambridge Isotope Laboratories, Inc.)) ...
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bioRxiv - Biochemistry 2021Quote: ... Tissue was incubated in Krebs-Ringer buffer (formulations used in each figure specified below below) supplemented with 5 mM glucose and [U-13C]-succinic acid (Cambridge isotope CLM-1571-0.1) or [U-13C]-malate (Cambridge isotope CLM-8065 ...
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bioRxiv - Biochemistry 2022Quote: ... MMA quantification was performed using 3 uL of plasma that was spiked in with [U-13C]-MMA (Cambridge Isotope Laboratories, cat# CLM-9426-PK) as previously described (31 ...
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bioRxiv - Immunology 2023Quote: WT and Slc2a5−/− P7 neonatal mice born to dams on HF or CD were injected intraperitoneally via the quadriceps with 4 g/kg [2-13C]-fructose (Cambridge Isotope Laboratories, CLM-1527) in PBS (20 mg in 200 μL PBS) ...
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bioRxiv - Cell Biology 2022Quote: ... Polar metabolites were extracted in ice cold 80% LC/MS grade methanol containing 15N and 13C fully-labeled amino acid standards (Cambridge Isotope Laboratories #MSK-A2-1.2). Tissues were homogenized by Bead Ruptor 24 (Omni International ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed twice with ice-cold 0.9% NaCl and polar metabolites extracted in ice cold 80% LC/MS grade methanol containing 15N and 13C fully-labeled amino acid standards (Cambridge Isotope Laboratories #MSK-A2-1.2). Extracts were vigorously shaken by vortex for 10 minutes at 4°C and spun at 14,000 rpm at 4°C for 10 minutes to remove insoluble cell debris ...
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bioRxiv - Cell Biology 2022Quote: ... Ultra pure bovine serum albumin (Cell Signalling Technology 9998) was used as a protein source and iPSDM were incubated with media containing 13C-labeled glucose (Cambridge Isotope Laboratories CLM-1396-5) and the described treatments for 1h before continuing with downstream sample processing ...
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bioRxiv - Genomics 2023Quote: ... followed by metabolite extraction in ice cold 80:20 LC/MS grade methanol: water buffer containing 15N and 13C fully-labeled amino acid standards (Cambridge Isotope Laboratories #MSK-A2-1.2). Samples were vortexed for 10 minutes at 4°C and then centrifuged at maximum speed (> 14,000g ...
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bioRxiv - Microbiology 2024Quote: ... spectroscopic data were obtained using Bruker NEO NMR system (1H: 600 MHz, 13C: 150 MHz) (Bruker, Billerica, MA) with CD3OD (Cambridge Isotope Laboratories, Inc., Tewksbury, MA). Chemical shifts are presented on a δ scale ...
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bioRxiv - Developmental Biology 2020Quote: Four aliquots of 200 μl of the bacterial suspension obtained as described above were added to 2 μl 10 mM 13C-citrulline (Lot No. PR-16536, Catalog No. CLM-4899-0, Cambridge Isotope Laboratories, Inc., Tewksbury, MA, USA). After 1 h of incubation ...
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bioRxiv - Microbiology 2021Quote: The ability of Thaumarchaeota to oxidize DON during growth on NH3 was assessed using 15N isotope tracers (98-99% 15N, Cambridge Isotope Laboratories). Axenic cultures were grown on SCM as described above except using 100 µM ammonium chloride and 20 µM pyruvate ...
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Synthetase and Hydrolase Specificity Collectively Excludes 2’-Deoxyguanosine from Bacterial AlarmonebioRxiv - Microbiology 2024Quote: ... 20 μM FeSO4-EDTA and 0.50 g/L regular NH4Cl (light medium) or 0.51 g/L NH4Cl (15N, 99%, Cambridge Isotope Laboratories, heavy medium) were used ...
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bioRxiv - Biophysics 2023Quote: ... They were then resuspended in 500 ml of M9 media supplemented with 100 mg of a desired 15N-labeled or 13C-labeled L-amino acid (Cambridge Isotope Labs, Inc. or Sigma-Aldrich, Inc.) as well as the remaining 19 unlabeled amino acids ...
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bioRxiv - Plant Biology 2021Quote: ... In darkness the sample was purged with N2 to minimise background 16O2 before a bubble of 18O2 (99% Cambridge Isotope Laboratories Inc, UK) was loaded into the stirring liquid ...
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bioRxiv - Plant Biology 2024Quote: ... A solution containing 15N was created by combining 15NKNO3 with a 99 atomic percent (15N) purity (Cambridge Isotope Laboratories, Inc., Andover, MA, USA) and unlabelled KNO3 (Merck ...
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bioRxiv - Physiology 2021Quote: ... continuous infusion of stable amino acid isotope [13C6]-phenylalanine (L-phenylalanine, ring-13C6, 99%, CLM-1055-MPT; Cambridge Isotope Laboratories, Inc., Tewksbury, MA, USA) was used for measurement of amino acid kinetics across the limb and incorporation of labeled phenylalanine into muscle as previously described [20] ...
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bioRxiv - Cell Biology 2023Quote: ... Taurine concentrations were then measured via LC-MS and analyzed the area of the exact mass for the corresponding ions of the targets as well as the intracellular labeled isotopic taurine levels (13C2, 99%; 15N, 98%, Cambridge isotope #CNLM-10253-PK).
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bioRxiv - Microbiology 2021Quote: ... The cosms were surface-irrigated at 7 am with 60 ml of either 19.85 mM 15NH4Cl (99% 15N, Cambridge Isotope Laboratories, Inc., MA, US, pH 5.47) or a 19.98 mM 15KNO3 (99% 15N ...
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bioRxiv - Plant Biology 2023Quote: ... and 1 g/L KNO3 or 1 g/L K15NO3 (Cambridge Isotope Laboratories), pH 5.8 ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1 g/L KNO3 or 1 g/L K15NO3 (Cambridge Isotope Laboratories), pH 5.8 ...
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bioRxiv - Biophysics 2023Quote: ... Uniformly-labeled 13C 15N Sup35NM samples were prepared by growing BL21(DES)-Rosetta Escherichia coli in the presence of M9 media with 2 g L-1 D-glucose 1H,13C6 and 1 g L-1 of 15N ammonium chloride (Cambridge Isotope Labs, Cambridge, MA). Purified ...
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bioRxiv - Physiology 2020Quote: ... constant infusion (0.0085 mg•kg-1•min-1) of [ring-13C6]-L-phenylalanine (Cambridge Isotope Laboratories, Tewksbury, MA) from 1000 to the end of the protocol ...
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bioRxiv - Biochemistry 2019Quote: ... with 1 g 15NH4Cl (Cambridge Isotope Laboratories) as the sole nitrogen source ...
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bioRxiv - Biochemistry 2022Quote: ... 1 g/L 15NH4Cl (Cambridge Isotope Laboratories) and 50 μg/mL kanamycin ...
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bioRxiv - Cancer Biology 2020Quote: ... Heavy lysine (1G: CLM-265-H-1) and arginine (1G: CNLM-291-H-1) were purchased from Cambridge Isotope Laboratories (CIL ...
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bioRxiv - Microbiology 2019Quote: ... 10 and 20 minutes and quenched in ice-cold acetonitrile:methanol (1:1) spiked with 0.1 mM 13C5,15N-L-glutamic acid as internal standard (Cambridge Isotope Laboratories). Samples were stored at -80 °C until RapidFire analysis ...
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bioRxiv - Cell Biology 2022Quote: ... TKO and Glut 5 were gavaged with a mix of 1:1 universally labeled fructose (U-13C6, Cambridge Isotope Laboratories) and regular glucose at 1g/kg body weight ...
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bioRxiv - Biochemistry 2019Quote: ... 13C6-glucose (Cambridge Isotope Laboratories CLM-1396-1), Vitamin B5-[13C6 15N2] (calcium pantothenate-[13C6 15N2] ...
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bioRxiv - Cancer Biology 2021Quote: ... PaTu-8902 and MIAPaCa-2 iDox-shGOT2.1 cells were cultured in media containing 1 mM unlabelled glucose and 1 mM U13C-Pyruvate (Cambridge Isotope Laboratories) for 16 hours.
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bioRxiv - Cancer Biology 2020Quote: ... TIF was mixed 1:1 with a solution of isotopically labeled glucose at a known concentration (Cambridge Isotope Laboratories, CLM-1396). Samples were extracted in 300 µl of ice cold HPLC grade methanol ...
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bioRxiv - Biochemistry 2019Quote: ... assay and immediately diluted (1:1) to a final protein concentration of 0.5 mg/mL with either 18O heavy (Cambridge Isotope Laboratories) or 16O light (Fisher ...
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bioRxiv - Plant Biology 2019Quote: ... or 15N MS medium (1/2 MS without nitrogen source [PhytoTechnology Laboratories], 15NH415NO3 [0.5 g/L, NLM-390-1, Cambridge Isotope Laboratory] ...
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bioRxiv - Biophysics 2020Quote: ... The rinsed cells were then used to inoculate 1 L of M9 medium supplemented with 1 g/L 15N-NH4Cl (Cambridge Isotope Labs), 4 g/L dextrose ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% FBS and heavy isotope-labeled amino acids (L-Lysine 13C6, L-Arginine 13C6) (CLM-2247-H-1, CLM-2265-H-1, Cambridge Isotope Laboratories). DAC-treated cells grown in the regular medium were considered as SILAC light counterparts.
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bioRxiv - Microbiology 2023Quote: ... or medium (84 mg L−1 13C6 L-arginine and 146 mg L−1 2H4 L-lysine) SILAC amino acids (Cambridge Isotope Laboratories). Medium SILAC labeled HeLa or HeLa S3 cells were used as spike-in control ...
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bioRxiv - Biochemistry 2023Quote: ... To the dried peptide, 300 μL of a 1:1 mixture 12 M DCl (35% w/v, Aldrich) and D2O (Cambridge Isotope Laboratory) containing 3% phenol (w/v ...
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bioRxiv - Immunology 2024Quote: ... the media was changed to DMEM media containing 1% dialyzed FBS or 1% dialyzed FBS supplemented with 2mM [U-13C8]-octanoate (Cambridge Isotope Laboratories). After 24h ...
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bioRxiv - Systems Biology 2021Quote: ... and 1 mM γ[18O4]-ATP (Cambridge Isotope Laboratory) in 30 °C for 1 hour ...
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bioRxiv - Immunology 2024Quote: ... and labeled (U-13C6, Cambridge Isotope Laboratories CLM-1396-1) or unlabeled glucose (Thermo Fisher Scientific ...