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Citations for Cambridge Isotope Labs :
1 - 50 of 169 citations for Dengue Virus VLP Serotypes 1 4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... using a pET-46 tobacco etch virus (TEV) protease-cleavable N-terminal hexahistidine tag fusion protein in M9 media supplemented with 15NH4Cl (Cambridge Isotope Labs, Inc.). Nurr1 LBD was eluted against a 500 mM imidazole gradient through a Ni-NTA column ...
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bioRxiv - Microbiology 2021Quote: ... Commercial reagents and standards for analysis were purchased from the following suppliers: Deuterated Dimethylsulfoxide (DMSO-d6, 99.9%) and 4,4-dimethyl-4-silapentane-1-sulfonic acid (Cambridge Isotope Laboratories ...
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bioRxiv - Microbiology 2021Quote: ... each of which was 25% labeled with 13C (i.e. mixed 1:4 with 25% H13CO3, 13C12H6O12, or CH313CO2, respectively; Cambridge Isotope Laboratories). All saltwater media was adapted from the Halohandbook and contained NaCl ...
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bioRxiv - Neuroscience 2023Quote: ... and heavy isotopes (4, 4, 5, 5-D4 L-lysine and 13C6 L-arginine, Cambridge Isotope Laboratories (CIL)) for the K4/R6 population ...
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bioRxiv - Biochemistry 2021Quote: ... and 0.798 mM L-(13C6,15N2)-lysine (Lys”8”)] or “medium” amino acids [0.398 mM L-(15N4)-arginine (Arg”4”) and 0.798 mM L-(D4)-lysine (Lys”4”)] (Cambridge Isotope Laboratories), and incubated for 2 hr as previously described elsewhere (Imami et al. ...
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bioRxiv - Biochemistry 2022Quote: ... a 100 mL LB starter culture treated with 100 μg/mL ampicillin was grown at 30 °C overnight and was used to inoculate 4× 1 L of M9 minimal medium containing 15NH4Cl and/or 13C6-glucose (Cambridge Isotope, Tewksbury, MA, USA). The cells were grown in this isotopically-enriched minimal media at 37 °C and shaken at 200 rpm until the OD600 reached 0.6-0.8 before a brief cold shock at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... Pellets were reconstituted in 60 μl acetonitrile:ultrapure water (4:1, containing 13C6/15N-isoleucine as internal standard, 40 μM, Cambridge Isotope Laboratories, CNLM-561-H) and vortexed vigorously ...
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bioRxiv - Biochemistry 2020Quote: ... and 0.798 mM L-(13C6,15N2)-lysine (Lys“8”)] or “medium” amino acids [0.398 mM L-(15N4)-arginine (Arg“4”) and 0.798 mM L-(D4)-lysine (Lys“4”)] (Cambridge Isotope Laboratories, Tewksbury, USA), and incubated for 2 h ...
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bioRxiv - Biochemistry 2019Quote: ... and deuterium oxide (Cambridge Isotope Laboratories, DLM-4-1000). Protein expression was induced with 100 μM IPTG and cultures were grown for 18 hours at 18°C ...
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bioRxiv - Biochemistry 2021Quote: ... 4 g/L 13C6-D-Glucose (Cambridge Isotope Laboratories) and 100 mg/L ampicillin at 37 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... or 4 mM U-[13C]-glutamine (Cambridge Isotope Laboratories) and 25 mM glucose (Sigma ...
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bioRxiv - Bioengineering 2023Quote: ... 600 µl of D2O (Cambridge Isotope Laboratories (DLM-4)) was added and homogenized ...
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bioRxiv - Cancer Biology 2023Quote: ... For the infusion of D2O (Cambridge Isotope, #DLM-4-50) and [2,2,3-2H]-serine (Cambridge Isotope ...
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bioRxiv - Biophysics 2022Quote: ... 10% D2O (v/v) (Cambridge Isotope Laboratories; Cat. No. DLM-4-25) was added to the sample for the spectrometer deuterium lock ...
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bioRxiv - Genetics 2020Quote: ... injection of 4 mmol/kg of 15N-labeled ammonium chloride (15NH4Cl, Cambridge Isotope Laboratories) 20 min before terminal blood collection via cardiac puncture ...
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bioRxiv - Molecular Biology 2020Quote: ... medium (M: R6K4 = +10Da) isotopes (13C on Arg, 4 D on Lys; Cambridge isotope laboratories ...
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bioRxiv - Cancer Biology 2020Quote: ... 10 mM U-13C-glucose or 4 mM U-13C-glutamine (Cambridge Isotope Laboratories), which included control (DMSO ...
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bioRxiv - Evolutionary Biology 2021Quote: ... D23-dodecanoid acid and 13C6-4-hydroxybenzoic acid (98-99% enrichment, Cambridge Isotope Laboratories, Inc.) as well as a control with sterile oat/poultry-rearing pellet-powder ...
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bioRxiv - Neuroscience 2022Quote: ... Catalog #:A456-4) prepared with Millipore water and spiked with deuterated isotope-labeled internal standards sourced from Cambridge Isotope Laboratories ...
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bioRxiv - Molecular Biology 2021Quote: ... a supplement of [13C6] L-arginine (Arg-6) and [2H4] L-lysine (Lys-4) was added (Cambridge Isotope Laboratories). For the heavy isotope containing medium ...
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bioRxiv - Cell Biology 2022Quote: ... The second plate was cultured in medium SILAC media with the same composition as above except Arg0 and Lys0 were replaced by L-Arginine [13C6] HCl (Arg-6) and L-ysine-4,4,5,5-d4 (Lys-4) (Cambridge Isotope Laboratories). The third plate was cultured in heavy SILAC media with the same composition except arginine and lysine were replaced by L-Arginine [13C6,D7,15N4]HCl (Arg-17 ...
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bioRxiv - Neuroscience 2023Quote: ... 5–10 μL of CSF or serum was extracted in 4:6:3 chloroform:methanol:water mixture supplemented with isotopically labeled T3 and T4 (at 100 nM, Cambridge Isotope Laboratories ...
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bioRxiv - Biochemistry 2020Quote: ... with non-labeled Xyl6 (200 µg) and Ac-CoA (4 mM) in 100 mM potassium phosphate buffer in D2O (99.9%; Cambridge Isotope Laboratories) for 6 h at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... 4 weeks after induction tumor-bearing mice and healthy littermate controls were treated with 15N2-glutamine (Cambridge Isotope Laboratory #NLM-1328-PK) dissolved in sterile phosphate buffered saline at 7.2mg/animal by tail vein injection as previously described (Lane et al. ...
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bioRxiv - Biochemistry 2022Quote: ... and 0.798 mM L-(13C6,15N2)-lysine (Lys”8”)] or “medium-heavy” amino acids [0.398 mM L-(13C6)-arginine (Arg”6”) and 0.798 mM L-(D4)-lysine (Lys”4”)] (Cambridge Isotope Laboratories, Tewksbury, USA). For chloramphenicol (CAP ...
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bioRxiv - Neuroscience 2023Quote: ... each rat was fed 1g of a pancake that contained 4 µl of [13C]-octanoic acid (Cambridge Isotope Laboratories, Inc, Tewksbury MA). After visually confirming the rats had eaten the entire pancake (in less than 5min or they were omitted from the study) ...
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bioRxiv - Cancer Biology 2024Quote: ... the media was removed and replaced with glutamine-free media supplemented with 10% dFBS and 4 mmol/L U-13C5-L-glutamine (CLM-1822-H, Cambridge Isotope Laboratories) for 2 hours prior to harvest (n = 3 per condition) ...
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bioRxiv - Plant Biology 2023Quote: ... and 1 g/L KNO3 or 1 g/L K15NO3 (Cambridge Isotope Laboratories), pH 5.8 ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1 g/L KNO3 or 1 g/L K15NO3 (Cambridge Isotope Laboratories), pH 5.8 ...
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bioRxiv - Biophysics 2023Quote: ... Uniformly-labeled 13C 15N Sup35NM samples were prepared by growing BL21(DES)-Rosetta Escherichia coli in the presence of M9 media with 2 g L-1 D-glucose 1H,13C6 and 1 g L-1 of 15N ammonium chloride (Cambridge Isotope Labs, Cambridge, MA). Purified ...
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bioRxiv - Immunology 2023Quote: WT and Slc2a5−/− P7 neonatal mice born to dams on HF or CD were injected intraperitoneally via the quadriceps with 4 g/kg [2-13C]-fructose (Cambridge Isotope Laboratories, CLM-1527) in PBS (20 mg in 200 μL PBS) ...
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bioRxiv - Immunology 2022Quote: ... BMDMs were infected by Mtb in DMEM medium supplemented with 10% dialyzed FBS and 4 mM of 50% U13C glutamine (Cambridge Isotope Laboratories, Inc, Tewksbury, MA) (2 mM labeled and 2 mM unlabeled glutamine ...
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bioRxiv - Cancer Biology 2023Quote: ... Tracing of glutamine was performed by supplementing no-glucose and no-glutamine media with 4 mM 13C5 L-glutamine (Cambridge Isotope Laboratories, Inc., CLM-1822-H) and 5 mM unlabeled glucose ...
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bioRxiv - Physiology 2020Quote: ... constant infusion (0.0085 mg•kg-1•min-1) of [ring-13C6]-L-phenylalanine (Cambridge Isotope Laboratories, Tewksbury, MA) from 1000 to the end of the protocol ...
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bioRxiv - Biochemistry 2019Quote: ... with 1 g 15NH4Cl (Cambridge Isotope Laboratories) as the sole nitrogen source ...
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bioRxiv - Bioengineering 2022Quote: ... 100 % 1-13C glucose (Cambridge Isotope Laboratories), or unlabelled glucose ...
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bioRxiv - Biochemistry 2022Quote: ... 1 g/L 15NH4Cl (Cambridge Isotope Laboratories) and 50 μg/mL kanamycin ...
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bioRxiv - Cancer Biology 2020Quote: ... Heavy lysine (1G: CLM-265-H-1) and arginine (1G: CNLM-291-H-1) were purchased from Cambridge Isotope Laboratories (CIL ...
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bioRxiv - Microbiology 2019Quote: ... 10 and 20 minutes and quenched in ice-cold acetonitrile:methanol (1:1) spiked with 0.1 mM 13C5,15N-L-glutamic acid as internal standard (Cambridge Isotope Laboratories). Samples were stored at -80 °C until RapidFire analysis ...
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bioRxiv - Cell Biology 2022Quote: ... TKO and Glut 5 were gavaged with a mix of 1:1 universally labeled fructose (U-13C6, Cambridge Isotope Laboratories) and regular glucose at 1g/kg body weight ...
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bioRxiv - Biochemistry 2019Quote: ... 13C6-glucose (Cambridge Isotope Laboratories CLM-1396-1), Vitamin B5-[13C6 15N2] (calcium pantothenate-[13C6 15N2] ...
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bioRxiv - Cancer Biology 2020Quote: ... 99% (Cambridge Isotope Laboratories, Inc., CLM-1396-1) and 2 mL of bovine pituitary extract ...
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bioRxiv - Biophysics 2021Quote: ... [1-13C]pyruvate (Cambridge Isotope Labs, Massachusetts, USA) with 15mM Ox063 (Oxford Instruments ...
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bioRxiv - Cancer Biology 2021Quote: ... PaTu-8902 and MIAPaCa-2 iDox-shGOT2.1 cells were cultured in media containing 1 mM unlabelled glucose and 1 mM U13C-Pyruvate (Cambridge Isotope Laboratories) for 16 hours.
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bioRxiv - Cancer Biology 2020Quote: ... TIF was mixed 1:1 with a solution of isotopically labeled glucose at a known concentration (Cambridge Isotope Laboratories, CLM-1396). Samples were extracted in 300 µl of ice cold HPLC grade methanol ...
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bioRxiv - Microbiology 2020Quote: ... irregularis was cultured in modified SC solid medium supplied with 1 mM neutralized [1-13C]myristic acid (Cambridge Isotope Laboratories, Inc.) for 2.5 months ...
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bioRxiv - Biochemistry 2019Quote: ... assay and immediately diluted (1:1) to a final protein concentration of 0.5 mg/mL with either 18O heavy (Cambridge Isotope Laboratories) or 16O light (Fisher ...
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bioRxiv - Plant Biology 2019Quote: ... or 15N MS medium (1/2 MS without nitrogen source [PhytoTechnology Laboratories], 15NH415NO3 [0.5 g/L, NLM-390-1, Cambridge Isotope Laboratory] ...
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bioRxiv - Biophysics 2020Quote: ... The rinsed cells were then used to inoculate 1 L of M9 medium supplemented with 1 g/L 15N-NH4Cl (Cambridge Isotope Labs), 4 g/L dextrose ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% FBS and heavy isotope-labeled amino acids (L-Lysine 13C6, L-Arginine 13C6) (CLM-2247-H-1, CLM-2265-H-1, Cambridge Isotope Laboratories). DAC-treated cells grown in the regular medium were considered as SILAC light counterparts.