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Citations for Cambridge Isotope Labs :
1 - 50 of 102 citations for Dengue Virus Serotype 4 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... using a pET-46 tobacco etch virus (TEV) protease-cleavable N-terminal hexahistidine tag fusion protein in M9 media supplemented with 15NH4Cl (Cambridge Isotope Labs, Inc.). Nurr1 LBD was eluted against a 500 mM imidazole gradient through a Ni-NTA column ...
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Conformational dynamics at microsecond timescale in the RNA-binding regions of dsRNA-binding domainsbioRxiv - Biophysics 2019Quote: ... the protein was prepared by growing the cells in the M9 media containing 15NH4Cl (Cambridge Isotope Laboratories) as a sole source of nitrogen.
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bioRxiv - Molecular Biology 2020Quote: ... protein was expressed in BL21/DE3 Escherichia coli cells using M9 minimal media supplemented with 15NH4Cl (Cambridge Isotope Laboratories) induced at an OD600 of 0.6 with 0.5 mM IPTG for 16 hours at 18°C ...
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bioRxiv - Neuroscience 2023Quote: ... and heavy isotopes (4, 4, 5, 5-D4 L-lysine and 13C6 L-arginine, Cambridge Isotope Laboratories (CIL)) for the K4/R6 population ...
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bioRxiv - Biochemistry 2021Quote: ... and 0.798 mM L-(13C6,15N2)-lysine (Lys”8”)] or “medium” amino acids [0.398 mM L-(15N4)-arginine (Arg”4”) and 0.798 mM L-(D4)-lysine (Lys”4”)] (Cambridge Isotope Laboratories), and incubated for 2 hr as previously described elsewhere (Imami et al. ...
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bioRxiv - Biophysics 2019Quote: ... and uniformly 15N- or 15N,13C-labeled proteins were purified from cells grown in M9 minimal media with 15N ammonium chloride and/or 13C D-glucose (Cambridge Isotope Labs) as the sole nitrogen and/or carbon source ...
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bioRxiv - Biochemistry 2020Quote: ... and 0.798 mM L-(13C6,15N2)-lysine (Lys“8”)] or “medium” amino acids [0.398 mM L-(15N4)-arginine (Arg“4”) and 0.798 mM L-(D4)-lysine (Lys“4”)] (Cambridge Isotope Laboratories, Tewksbury, USA), and incubated for 2 h ...
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bioRxiv - Biochemistry 2019Quote: ... and deuterium oxide (Cambridge Isotope Laboratories, DLM-4-1000). Protein expression was induced with 100 μM IPTG and cultures were grown for 18 hours at 18°C ...
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bioRxiv - Biochemistry 2021Quote: ... 4 g/L 13C6-D-Glucose (Cambridge Isotope Laboratories) and 100 mg/L ampicillin at 37 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... or 4 mM U-[13C]-glutamine (Cambridge Isotope Laboratories) and 25 mM glucose (Sigma ...
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bioRxiv - Bioengineering 2023Quote: ... 600 µl of D2O (Cambridge Isotope Laboratories (DLM-4)) was added and homogenized ...
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bioRxiv - Biochemistry 2021Quote: ... cells while expression was carried out in LB media for non-labeled protein and M9 minimal media containing 15NH4Cl (Cambridge Isotope Laboratories, Tewksbury, MA) for 15N ...
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bioRxiv - Cancer Biology 2023Quote: ... For the infusion of D2O (Cambridge Isotope, #DLM-4-50) and [2,2,3-2H]-serine (Cambridge Isotope ...
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bioRxiv - Biophysics 2022Quote: ... 10% D2O (v/v) (Cambridge Isotope Laboratories; Cat. No. DLM-4-25) was added to the sample for the spectrometer deuterium lock ...
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bioRxiv - Genetics 2020Quote: ... injection of 4 mmol/kg of 15N-labeled ammonium chloride (15NH4Cl, Cambridge Isotope Laboratories) 20 min before terminal blood collection via cardiac puncture ...
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bioRxiv - Molecular Biology 2020Quote: ... medium (M: R6K4 = +10Da) isotopes (13C on Arg, 4 D on Lys; Cambridge isotope laboratories ...
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bioRxiv - Cancer Biology 2020Quote: ... 10 mM U-13C-glucose or 4 mM U-13C-glutamine (Cambridge Isotope Laboratories), which included control (DMSO ...
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bioRxiv - Biochemistry 2021Quote: ... protein was expressed in M9 minimal media with 15N-NH4Cl (Cambridge Isotope labs) as the sole nitrogen source instead of 2xYT media ...
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bioRxiv - Evolutionary Biology 2021Quote: ... D23-dodecanoid acid and 13C6-4-hydroxybenzoic acid (98-99% enrichment, Cambridge Isotope Laboratories, Inc.) as well as a control with sterile oat/poultry-rearing pellet-powder ...
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bioRxiv - Microbiology 2023Quote: ... We spiked 13C and 15N labeled algal AAs or protein (Cambridge Isotope Laboratories, Inc., MA) into late-log phase ...
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bioRxiv - Neuroscience 2022Quote: ... Catalog #:A456-4) prepared with Millipore water and spiked with deuterated isotope-labeled internal standards sourced from Cambridge Isotope Laboratories ...
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bioRxiv - Microbiology 2021Quote: ... Commercial reagents and standards for analysis were purchased from the following suppliers: Deuterated Dimethylsulfoxide (DMSO-d6, 99.9%) and 4,4-dimethyl-4-silapentane-1-sulfonic acid (Cambridge Isotope Laboratories ...
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bioRxiv - Molecular Biology 2021Quote: ... a supplement of [13C6] L-arginine (Arg-6) and [2H4] L-lysine (Lys-4) was added (Cambridge Isotope Laboratories). For the heavy isotope containing medium ...
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bioRxiv - Cell Biology 2022Quote: ... The second plate was cultured in medium SILAC media with the same composition as above except Arg0 and Lys0 were replaced by L-Arginine [13C6] HCl (Arg-6) and L-ysine-4,4,5,5-d4 (Lys-4) (Cambridge Isotope Laboratories). The third plate was cultured in heavy SILAC media with the same composition except arginine and lysine were replaced by L-Arginine [13C6,D7,15N4]HCl (Arg-17 ...
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bioRxiv - Neuroscience 2023Quote: ... 5–10 μL of CSF or serum was extracted in 4:6:3 chloroform:methanol:water mixture supplemented with isotopically labeled T3 and T4 (at 100 nM, Cambridge Isotope Laboratories ...
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bioRxiv - Molecular Biology 2021Quote: ... and proteins were produced in the M9 media supplemented with 2 g/L [15N]NH4Cl (Cambridge Isotope Laboratories) and 3 g/L d-[13C6]glucose (Cambridge Isotope Laboratories) ...
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bioRxiv - Biochemistry 2020Quote: ... with non-labeled Xyl6 (200 µg) and Ac-CoA (4 mM) in 100 mM potassium phosphate buffer in D2O (99.9%; Cambridge Isotope Laboratories) for 6 h at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... each of which was 25% labeled with 13C (i.e. mixed 1:4 with 25% H13CO3, 13C12H6O12, or CH313CO2, respectively; Cambridge Isotope Laboratories). All saltwater media was adapted from the Halohandbook and contained NaCl ...
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bioRxiv - Cancer Biology 2022Quote: ... 4 weeks after induction tumor-bearing mice and healthy littermate controls were treated with 15N2-glutamine (Cambridge Isotope Laboratory #NLM-1328-PK) dissolved in sterile phosphate buffered saline at 7.2mg/animal by tail vein injection as previously described (Lane et al. ...
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bioRxiv - Biochemistry 2022Quote: ... and 0.798 mM L-(13C6,15N2)-lysine (Lys”8”)] or “medium-heavy” amino acids [0.398 mM L-(13C6)-arginine (Arg”6”) and 0.798 mM L-(D4)-lysine (Lys”4”)] (Cambridge Isotope Laboratories, Tewksbury, USA). For chloramphenicol (CAP ...
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bioRxiv - Neuroscience 2023Quote: ... each rat was fed 1g of a pancake that contained 4 µl of [13C]-octanoic acid (Cambridge Isotope Laboratories, Inc, Tewksbury MA). After visually confirming the rats had eaten the entire pancake (in less than 5min or they were omitted from the study) ...
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bioRxiv - Cancer Biology 2024Quote: ... the media was removed and replaced with glutamine-free media supplemented with 10% dFBS and 4 mmol/L U-13C5-L-glutamine (CLM-1822-H, Cambridge Isotope Laboratories) for 2 hours prior to harvest (n = 3 per condition) ...
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bioRxiv - Biochemistry 2019Quote: ... assay and immediately diluted (1:1) to a final protein concentration of 0.5 mg/mL with either 18O heavy (Cambridge Isotope Laboratories) or 16O light (Fisher ...
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bioRxiv - Biophysics 2023Quote: ... 15N-labeled and 15N-13C labeled (as required) TRBP2-dsRBD2 protein was prepared using 15NH4Cl and 13C-glucose (Cambridge Isotope Laboratories) as a sole source of nitrogen and carbon ...
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bioRxiv - Biophysics 2024Quote: ... to obtain the 15N uniformly single labelled protein or in a media containing 15NH4Cl (0.1% w/v) and 13C-labelled glucose (0.4% w/v) (Cambridge isotope Laboratories, Inc.) for 15N and 13C uniformly double labelled protein ...
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bioRxiv - Biochemistry 2022Quote: ... a 100 mL LB starter culture treated with 100 μg/mL ampicillin was grown at 30 °C overnight and was used to inoculate 4× 1 L of M9 minimal medium containing 15NH4Cl and/or 13C6-glucose (Cambridge Isotope, Tewksbury, MA, USA). The cells were grown in this isotopically-enriched minimal media at 37 °C and shaken at 200 rpm until the OD600 reached 0.6-0.8 before a brief cold shock at 4 °C ...
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bioRxiv - Immunology 2023Quote: WT and Slc2a5−/− P7 neonatal mice born to dams on HF or CD were injected intraperitoneally via the quadriceps with 4 g/kg [2-13C]-fructose (Cambridge Isotope Laboratories, CLM-1527) in PBS (20 mg in 200 μL PBS) ...
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bioRxiv - Microbiology 2024Quote: ... Pellets were reconstituted in 60 μl acetonitrile:ultrapure water (4:1, containing 13C6/15N-isoleucine as internal standard, 40 μM, Cambridge Isotope Laboratories, CNLM-561-H) and vortexed vigorously ...
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bioRxiv - Biochemistry 2021Quote: ... then 10% of bead volume was lysed with 1% Triton buffer for protein extracts and the remaining 90% was extracted in 80% methanol containing heavy labeled amino acid standards (Cambridge Isotope Labs) on a rotator shaker for 10□min at 4□°C.
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bioRxiv - Biochemistry 2023Quote: ... 15N-labeled protein samples were grown in 1L minimal media containing 3 g/L 15N ammonium chloride (Cambridge Isotope Laboratories, Tweksbury, MA) and unlabeled protein samples were grown in 1L super broth media ...
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bioRxiv - Immunology 2022Quote: ... BMDMs were infected by Mtb in DMEM medium supplemented with 10% dialyzed FBS and 4 mM of 50% U13C glutamine (Cambridge Isotope Laboratories, Inc, Tewksbury, MA) (2 mM labeled and 2 mM unlabeled glutamine ...
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bioRxiv - Biochemistry 2022Quote: ... and sortase A-compatible proteins were either expressed in Terrific Broth (TB) media or M9 media supplemented with 13C-glucose and/or 15NH4Cl (Cambridge Isotope Labs, Inc.) followed by protein induction with 0.5 mM isopropyl ß-D-1-thiogalactopyranoside (Gold Biotechnology ...
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bioRxiv - Biophysics 2024Quote: ... the cells were transferred to fresh LB medium for the overexpression of the unlabeled protein or to M9 minimal medium supplied with 15NH4Cl (Cambridge Isotope Laboratories Inc.) as the only nitrogen source for the labeled protein overexpression ...
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bioRxiv - Cancer Biology 2023Quote: ... Tracing of glutamine was performed by supplementing no-glucose and no-glutamine media with 4 mM 13C5 L-glutamine (Cambridge Isotope Laboratories, Inc., CLM-1822-H) and 5 mM unlabeled glucose ...
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bioRxiv - Cell Biology 2022Quote: ... Ultra pure bovine serum albumin (Cell Signalling Technology 9998) was used as a protein source and iPSDM were incubated with media containing 13C-labeled glucose (Cambridge Isotope Laboratories CLM-1396-5) and the described treatments for 1h before continuing with downstream sample processing ...
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bioRxiv - Biochemistry 2021Quote: ... BioExpress cell growth media (Cambridge Isotope Laboratories Inc., Andover, MA) was added (2×100 mL of 10X U-15N ...
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Domain-specific quantification of prion protein in cerebrospinal fluid by targeted mass spectrometrybioRxiv - Neuroscience 2019Quote: ... coli in 15N cell growth medium (Cambridge Isotope Laboratories CGM-1000-N) induced with 15N auto-induction medium (Millipore 71759-3) ...
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bioRxiv - Biochemistry 2023Quote: ... 10 mL Bioexpress Cell Growth Media (Cambridge Isotope Laboratories, Tewksbury, MA, USA), 5 mL Gibco™ MEM Vitamin Solution (100x) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were supplemented with fresh 5.5 mM [U-13C6] glucose (Cambridge Isotope Laboratories). After 24 hr incubation ...
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bioRxiv - Cell Biology 2019Quote: ... cells were cultured in 10 mM [U-13C6]glucose (Cambridge Isotope Laboratories, Inc.) containing either 1000 µM serine/glycine or no serine/glycine for 48 h prior to extraction ...