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Citations for Cambridge Isotope Labs :
1 - 50 of 161 citations for Dengue Virus Serotype 1 Envelope Protein Human Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... using a pET-46 tobacco etch virus (TEV) protease-cleavable N-terminal hexahistidine tag fusion protein in M9 media supplemented with 15NH4Cl (Cambridge Isotope Labs, Inc.). Nurr1 LBD was eluted against a 500 mM imidazole gradient through a Ni-NTA column ...
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bioRxiv - Biochemistry 2019Quote: ... assay and immediately diluted (1:1) to a final protein concentration of 0.5 mg/mL with either 18O heavy (Cambridge Isotope Laboratories) or 16O light (Fisher ...
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bioRxiv - Biochemistry 2021Quote: ... protein was expressed in M9 minimal media with 15N-NH4Cl (Cambridge Isotope labs) as the sole nitrogen source instead of 2xYT media ...
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bioRxiv - Cell Biology 2019Quote: 3 μL of human plasma were spiked with 3 μL amino acid isotope labelled internal standards (Cambridge Isotope Laboratories, #MSK-A2-1.2) and extracted with 250 μL –20 °C methanol for 10 min and centrifuged at 4°C for 10 min at 15 000 g ...
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bioRxiv - Immunology 2021Quote: ... complete CTL:AIM-V labeling medium consisted of glucose-free CTL:AIM-V with 5% heat-inactivated human AB serum supplemented with 11 mM uniformly labeled 13C-glucose ([U-13C]glucose) (Cambridge Isotope Laboratories). Glucose-free ImmunoCult™-XF T Cell Expansion Medium (STEMCELL Technologies ...
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bioRxiv - Microbiology 2023Quote: ... We spiked 13C and 15N labeled algal AAs or protein (Cambridge Isotope Laboratories, Inc., MA) into late-log phase ...
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Conformational dynamics at microsecond timescale in the RNA-binding regions of dsRNA-binding domainsbioRxiv - Biophysics 2019Quote: ... the protein was prepared by growing the cells in the M9 media containing 15NH4Cl (Cambridge Isotope Laboratories) as a sole source of nitrogen.
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bioRxiv - Molecular Biology 2021Quote: ... and proteins were produced in the M9 media supplemented with 2 g/L [15N]NH4Cl (Cambridge Isotope Laboratories) and 3 g/L d-[13C6]glucose (Cambridge Isotope Laboratories) ...
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bioRxiv - Microbiology 2023Quote: ... 250 µL of each human CVL sample was added to 750 µL of methanol containing 200 nM 13C18-OA (Cambridge Isotope Laboratories, Inc. #CLM-460-PK), incubated on ice for 15 min ...
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bioRxiv - Molecular Biology 2020Quote: ... protein was expressed in BL21/DE3 Escherichia coli cells using M9 minimal media supplemented with 15NH4Cl (Cambridge Isotope Laboratories) induced at an OD600 of 0.6 with 0.5 mM IPTG for 16 hours at 18°C ...
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bioRxiv - Plant Biology 2023Quote: ... and 1 g/L KNO3 or 1 g/L K15NO3 (Cambridge Isotope Laboratories), pH 5.8 ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1 g/L KNO3 or 1 g/L K15NO3 (Cambridge Isotope Laboratories), pH 5.8 ...
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bioRxiv - Biophysics 2023Quote: ... 15N-labeled and 15N-13C labeled (as required) TRBP2-dsRBD2 protein was prepared using 15NH4Cl and 13C-glucose (Cambridge Isotope Laboratories) as a sole source of nitrogen and carbon ...
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bioRxiv - Biophysics 2024Quote: ... to obtain the 15N uniformly single labelled protein or in a media containing 15NH4Cl (0.1% w/v) and 13C-labelled glucose (0.4% w/v) (Cambridge isotope Laboratories, Inc.) for 15N and 13C uniformly double labelled protein ...
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bioRxiv - Biophysics 2023Quote: ... Uniformly-labeled 13C 15N Sup35NM samples were prepared by growing BL21(DES)-Rosetta Escherichia coli in the presence of M9 media with 2 g L-1 D-glucose 1H,13C6 and 1 g L-1 of 15N ammonium chloride (Cambridge Isotope Labs, Cambridge, MA). Purified ...
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bioRxiv - Biophysics 2019Quote: ... and uniformly 15N- or 15N,13C-labeled proteins were purified from cells grown in M9 minimal media with 15N ammonium chloride and/or 13C D-glucose (Cambridge Isotope Labs) as the sole nitrogen and/or carbon source ...
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bioRxiv - Biochemistry 2021Quote: ... then 10% of bead volume was lysed with 1% Triton buffer for protein extracts and the remaining 90% was extracted in 80% methanol containing heavy labeled amino acid standards (Cambridge Isotope Labs) on a rotator shaker for 10□min at 4□°C.
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bioRxiv - Biochemistry 2023Quote: ... 15N-labeled protein samples were grown in 1L minimal media containing 3 g/L 15N ammonium chloride (Cambridge Isotope Laboratories, Tweksbury, MA) and unlabeled protein samples were grown in 1L super broth media ...
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bioRxiv - Biochemistry 2022Quote: ... and sortase A-compatible proteins were either expressed in Terrific Broth (TB) media or M9 media supplemented with 13C-glucose and/or 15NH4Cl (Cambridge Isotope Labs, Inc.) followed by protein induction with 0.5 mM isopropyl ß-D-1-thiogalactopyranoside (Gold Biotechnology ...
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bioRxiv - Biophysics 2024Quote: ... the cells were transferred to fresh LB medium for the overexpression of the unlabeled protein or to M9 minimal medium supplied with 15NH4Cl (Cambridge Isotope Laboratories Inc.) as the only nitrogen source for the labeled protein overexpression ...
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bioRxiv - Biochemistry 2021Quote: ... cells while expression was carried out in LB media for non-labeled protein and M9 minimal media containing 15NH4Cl (Cambridge Isotope Laboratories, Tewksbury, MA) for 15N ...
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bioRxiv - Physiology 2020Quote: ... constant infusion (0.0085 mg•kg-1•min-1) of [ring-13C6]-L-phenylalanine (Cambridge Isotope Laboratories, Tewksbury, MA) from 1000 to the end of the protocol ...
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bioRxiv - Biochemistry 2019Quote: ... with 1 g 15NH4Cl (Cambridge Isotope Laboratories) as the sole nitrogen source ...
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bioRxiv - Bioengineering 2022Quote: ... 100 % 1-13C glucose (Cambridge Isotope Laboratories), or unlabelled glucose ...
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bioRxiv - Biochemistry 2022Quote: ... 1 g/L 15NH4Cl (Cambridge Isotope Laboratories) and 50 μg/mL kanamycin ...
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bioRxiv - Cell Biology 2022Quote: ... Ultra pure bovine serum albumin (Cell Signalling Technology 9998) was used as a protein source and iPSDM were incubated with media containing 13C-labeled glucose (Cambridge Isotope Laboratories CLM-1396-5) and the described treatments for 1h before continuing with downstream sample processing ...
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bioRxiv - Cancer Biology 2020Quote: ... Heavy lysine (1G: CLM-265-H-1) and arginine (1G: CNLM-291-H-1) were purchased from Cambridge Isotope Laboratories (CIL ...
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bioRxiv - Microbiology 2019Quote: ... 10 and 20 minutes and quenched in ice-cold acetonitrile:methanol (1:1) spiked with 0.1 mM 13C5,15N-L-glutamic acid as internal standard (Cambridge Isotope Laboratories). Samples were stored at -80 °C until RapidFire analysis ...
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bioRxiv - Cell Biology 2022Quote: ... TKO and Glut 5 were gavaged with a mix of 1:1 universally labeled fructose (U-13C6, Cambridge Isotope Laboratories) and regular glucose at 1g/kg body weight ...
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bioRxiv - Biochemistry 2019Quote: ... 13C6-glucose (Cambridge Isotope Laboratories CLM-1396-1), Vitamin B5-[13C6 15N2] (calcium pantothenate-[13C6 15N2] ...
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bioRxiv - Cancer Biology 2020Quote: ... 99% (Cambridge Isotope Laboratories, Inc., CLM-1396-1) and 2 mL of bovine pituitary extract ...
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bioRxiv - Biophysics 2021Quote: ... [1-13C]pyruvate (Cambridge Isotope Labs, Massachusetts, USA) with 15mM Ox063 (Oxford Instruments ...
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bioRxiv - Cancer Biology 2021Quote: ... PaTu-8902 and MIAPaCa-2 iDox-shGOT2.1 cells were cultured in media containing 1 mM unlabelled glucose and 1 mM U13C-Pyruvate (Cambridge Isotope Laboratories) for 16 hours.
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bioRxiv - Cancer Biology 2020Quote: ... TIF was mixed 1:1 with a solution of isotopically labeled glucose at a known concentration (Cambridge Isotope Laboratories, CLM-1396). Samples were extracted in 300 µl of ice cold HPLC grade methanol ...
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bioRxiv - Microbiology 2020Quote: ... irregularis was cultured in modified SC solid medium supplied with 1 mM neutralized [1-13C]myristic acid (Cambridge Isotope Laboratories, Inc.) for 2.5 months ...
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bioRxiv - Plant Biology 2019Quote: ... or 15N MS medium (1/2 MS without nitrogen source [PhytoTechnology Laboratories], 15NH415NO3 [0.5 g/L, NLM-390-1, Cambridge Isotope Laboratory] ...
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bioRxiv - Biophysics 2020Quote: ... The rinsed cells were then used to inoculate 1 L of M9 medium supplemented with 1 g/L 15N-NH4Cl (Cambridge Isotope Labs), 4 g/L dextrose ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% FBS and heavy isotope-labeled amino acids (L-Lysine 13C6, L-Arginine 13C6) (CLM-2247-H-1, CLM-2265-H-1, Cambridge Isotope Laboratories). DAC-treated cells grown in the regular medium were considered as SILAC light counterparts.
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bioRxiv - Microbiology 2023Quote: ... or medium (84 mg L−1 13C6 L-arginine and 146 mg L−1 2H4 L-lysine) SILAC amino acids (Cambridge Isotope Laboratories). Medium SILAC labeled HeLa or HeLa S3 cells were used as spike-in control ...
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bioRxiv - Biochemistry 2023Quote: ... To the dried peptide, 300 μL of a 1:1 mixture 12 M DCl (35% w/v, Aldrich) and D2O (Cambridge Isotope Laboratory) containing 3% phenol (w/v ...
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bioRxiv - Immunology 2024Quote: ... the media was changed to DMEM media containing 1% dialyzed FBS or 1% dialyzed FBS supplemented with 2mM [U-13C8]-octanoate (Cambridge Isotope Laboratories). After 24h ...
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bioRxiv - Systems Biology 2021Quote: ... and 1 mM γ[18O4]-ATP (Cambridge Isotope Laboratory) in 30 °C for 1 hour ...
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bioRxiv - Plant Biology 2022Quote: ... or 1 mM 13C-ring labeled phenylalanine (Cambridge Isotope Laboratories ...
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bioRxiv - Microbiology 2023Quote: ... and [U-13C]1-propanol (Cambridge Isotope Laboratories, Inc.). Ethyl [U-13C]2-hydroxybutyrate (Cambridge Isotope Laboratories ...
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bioRxiv - Biochemistry 2021Quote: ... 13C-1 labelled polyM was produced from fermentation of the same strain using 13C-1 (99 % 13C) D-fructose (Cambridge Isotope Laboratories, USA). This substrate was further subjected to acid hydrolysis as described elsewhere (39 ...
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bioRxiv - Immunology 2024Quote: ... and labeled (U-13C6, Cambridge Isotope Laboratories CLM-1396-1) or unlabeled glucose (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... 99% [2,3-13C] sodium succinate or an equimolar molar 1:1 mixture of [U-13C] sodium succinate (obtained from Cambridge Isotope Laboratories, Inc., Andover, MA, USA) and naturally labelled sodium succinate.
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bioRxiv - Cancer Biology 2023Quote: ... 10 µL of urine was mixed with 90 µL of methanol: water (1:1) and internal standards (Labeled tryptophan (CNLM-2475-H-PK, Cambridge Isotope Laboratories, Inc., Andover, MA), zeatine and inosine ...
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bioRxiv - Immunology 2020Quote: ... [1-13C] pyruvic acid (30 μL, Cambridge Isotope Laboratories, Tewksbury, MA) containing 15 mM OX063 and 2.5 mM gadolinium chelate ProHance (Bracco Diagnostics ...
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bioRxiv - Synthetic Biology 2022Quote: ... 1-13C-sodium acetate (Cambridge Isotope Laboratories Inc., Andover, MA, USA) and/or 13CO2 (Cambridge Isotope Laboratories Inc. ...