Labshake search
Citations for Cambridge Isotope Labs :
51 - 79 of 79 citations for 7 Bromo 5 fluoro 3 methyl 1H indole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... The dry MeOH extract (∼5 mg) was dissolved in 1 ml deuterated methanol (CD3OD; Cambridge Isotope Laboratories) for analysis by proton nuclear magnetic resonance (1H NMR ...
-
bioRxiv - Microbiology 2024Quote: ... they were diluted 1:5 with an internal standard mixture (L-Adenine-8-13C (Cambridge Isotope Lab), L-Phenyl-d5-Alanine-2,3,3-d3 (Cambridge Isotope Lab) ...
-
bioRxiv - Molecular Biology 2021Quote: ... expression was carried out in M9 minimal media supplemented with 3 g/L (13C6, 99%)-D-glucose and/or 1 g/l (15N, 99%)-NH4Cl (Cambridge Isotope Laboratories). To generate isotopically labeled rad6-A126T ...
-
bioRxiv - Biophysics 2020Quote: ... harvested at an OD600nm of 3 and resuspended in 2 L of M9 minimal medium with isotopic enrichment (1 g/L of 15NH4Cl and/or 3 g/L of 13C6-glucose, Cambridge Isotope Laboratories). After 30-45 min ...
-
bioRxiv - Biochemistry 2023Quote: ... 15N-labeled protein samples were grown in 1L minimal media containing 3 g/L 15N ammonium chloride (Cambridge Isotope Laboratories, Tweksbury, MA) and unlabeled protein samples were grown in 1L super broth media ...
-
bioRxiv - Developmental Biology 2021Quote: ... Islets were then incubated on a rotator plate at 95 RPM for 90 minutes at 37°C and 5% CO2 before being transferred to Eppendorf tubes and the basal KRB exchanged for a 0.9 ml volume of KRB containing either 3 mM (low) or 17 mM (high) [U-13C6] glucose (Cambridge Isotope Laboratories, CLM 1396). Islets were then incubated for 1 hour at 37°C and 5% CO2 ...
-
bioRxiv - Biophysics 2024Quote: ... The cells were used to inoculate 2L of H2O-based M9 media supplemented with 3 g L-1 of [U-13C6] d-glucose (Cambridge Isotope Laboratories, CIL) and 2 g L-1 of [15N] ammonium chloride (Cambridge Isotope Laboratories ...
-
bioRxiv - Cancer Biology 2022Quote: ... the dried pellet was dissolved in 550 µl D2O containing 0.01 mg/ml Sodium 2,2-Dimethyl-2-Silapentane-5-Sulfonate (DSS; Cambridge Isotope). The samples were then vortexed and centrifuged at 16000 rpm for 12 min ...
-
bioRxiv - Cell Biology 2023Quote: ... The media was exchanged for media with or without 5 mM 13C6-Glucose (Cambridge Isotope Laboratories Inc., CLM-1396) in HPLM or DMEM based MCF10A media for 2 h (for 13C6-Glucose media ...
-
bioRxiv - Biochemistry 2023Quote: ... cells were grown in the standard HMI-11 medium supplemented with 5 mM 13C-U-glycerol (Cambridge Isotope Laboratories). For the experiment with Δfbp.sbp/RNAiPFK ...
-
bioRxiv - Biochemistry 2022Quote: ... MMA quantification was performed using 3 uL of plasma that was spiked in with [U-13C]-MMA (Cambridge Isotope Laboratories, cat# CLM-9426-PK) as previously described (31 ...
-
bioRxiv - Biochemistry 2021Quote: ... 25.9 mM NaHCO3) supplemented with 5 mM glucose and 50 μM [U-13C]-succinic acid (Cambridge isotope CLM-1571-0.1). This buffer was pre-equilibrated at 37°C ...
-
bioRxiv - Biochemistry 2021Quote: ... containing 0.11 mM of the internal standard DSS (sodium 2,2-dimethyl-2-silapentane-5-sulfonate, D6, 98%; Cambridge Isotope Laboratories) at pH 7.0 and vortex mixed for <1 min prior to transfer into 1.7 mm SampleJet NMR tubes ...
-
bioRxiv - Biochemistry 2021Quote: ... 5 mM MgCl2, 0.5 mM EDTA, 0.5 mM ATP, 1 mM DTT, 10% glycerol [v/v], D2O [Cambridge Isotope Laboratories]) at 25°C and quenched by a 2-fold dilution with ice-cold quench buffer (0.8 M guanidine hydrochloride ...
-
bioRxiv - Cell Biology 2022Quote: ... TKO and Glut 5 were gavaged with a mix of 1:1 universally labeled fructose (U-13C6, Cambridge Isotope Laboratories) and regular glucose at 1g/kg body weight ...
-
bioRxiv - Immunology 2023Quote: ... On the final day all media conditions were replaced with 5 mM [U-13C]-fructose (Cambridge Isotope Laboratories, CLM-1553). Metabolites were extracted using 80% methanol 24 hours later ...
-
bioRxiv - Biophysics 2021Quote: ... and the cell pellet was resuspended in 0.5L of M9 minimal media containing 15NH2Cl with either 13C-glucose or 5% 13C-glucose (Cambridge Isotope Laboratories). Incubation continued for about 1hr at 35 C when expression was induced with 1 mM IPTG ...
-
bioRxiv - Microbiology 2022Quote: ... or 300 mL (algal cultures) of filtrate was collected in glass Erlenmeyer flasks and spiked with 5 μL of benzoate-d5 (98%, Cambridge Isotope Laboratries ...
-
bioRxiv - Systems Biology 2022Quote: ... in a 100 mM sodium phosphate buffered solution with 0.11 mM sodium 2,2-dimethyl-2-silapentane-5-sulfonate (DSS-D6; 98%; Cambridge Isotope Laboratories, Inc.). Once dry ...
-
bioRxiv - Cancer Biology 2023Quote: ... no-glucose and no-glutamine media was supplemented with 5 mM of 13C6 D-Glucose (Cambridge Isotope Laboratories, Inc., CLM-1396) and 4 mM unlabeled glutamine ...
-
bioRxiv - Immunology 2021Quote: ... complete CTL:AIM-V labeling medium consisted of glucose-free CTL:AIM-V with 5% heat-inactivated human AB serum supplemented with 11 mM uniformly labeled 13C-glucose ([U-13C]glucose) (Cambridge Isotope Laboratories). Glucose-free ImmunoCult™-XF T Cell Expansion Medium (STEMCELL Technologies ...
-
bioRxiv - Cell Biology 2023Quote: Mice were injected intraperitoneally with AZD 5 mg/kg, 2 h prior to intravenous tail vein injection with [U13C] succinate (10 mg/kg, 2 min) (Cambridge Isotope Laboratories). Intravenous injections were performed as a bolus over 20 s ...
-
bioRxiv - Microbiology 2024Quote: ... 100 µL of each strain was transferred from the unlabeled glucose M9 media to 5 mL of M9 media with 50% 13C glucose (99% 13C, Cambridge Isotope Laboratory) and 50% natural abundance glucose and allowed to grow overnight at room temperature ...
-
bioRxiv - Plant Biology 2023Quote: ... and detached leaves were fed continuously through the petiole with an aqueous solution of either 5 mM isotopically labelled L-lysine (L-lysine·2HCl [13C6, 15N2], Cambridge Isotope Laboratories, Inc.) or 5 mM unlabeled L-lysine (Sigma-Aldrich) ...
-
bioRxiv - Physiology 2023Quote: ... hearts were perfused for 20 minutes with a modified KH-buffer containing uniformly labelled glucose (5 mmol/L [U-13C]glucose, Cambridge Isotope Laboratories, Inc.) instead of standard glucose ...
-
bioRxiv - Biochemistry 2021Quote: ... Tissue was incubated in Krebs-Ringer buffer (formulations used in each figure specified below below) supplemented with 5 mM glucose and [U-13C]-succinic acid (Cambridge isotope CLM-1571-0.1) or [U-13C]-malate (Cambridge isotope CLM-8065 ...
-
bioRxiv - Cancer Biology 2023Quote: 100 µL of each CSF sample was spiked with 5 µL of a mixture of stable isotope-labeled standards (Cambridge Isotope Labs, Tewksbury, MA). An aliquot of pre-chilled methanol was added to each sample for a final composition of 80% methanol to extract metabolites and precipitate proteins ...
-
bioRxiv - Cancer Biology 2019Quote: ... The cells were rinsed twice with PBS and replenished at time point 0 by either a glucose-free DMEM medium with the addition of 5 mM 13C6 glucose (Cambridge Isotope Laboratories Inc., Tewksbury, MA), 10% dialyzed FBS and 1% penicillin/streptomycin ...
-
bioRxiv - Cell Biology 2022Quote: ... Ultra pure bovine serum albumin (Cell Signalling Technology 9998) was used as a protein source and iPSDM were incubated with media containing 13C-labeled glucose (Cambridge Isotope Laboratories CLM-1396-5) and the described treatments for 1h before continuing with downstream sample processing ...