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Citations for Cambridge Isotope Labs :
51 - 100 of 263 citations for 7 8 9 10 TETRAHYDRO 6H AZEPINO 2 1 B QUINAZOLIN 12 YLIDENEAMINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: HDX samples of I552A SLO-1 were exchanged as a function of time by a 10-fold dilution into D2O (99% D; Cambridge Isotope) buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... Omental tumor biopsies from HGSOC patients were sectioned (0.5-1 mm) and incubated in DMEM supplemented with 10 mM [U-13C]-glucose (Cambridge Isotope Laboratories) and 2 mM glutamine ...
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bioRxiv - Cancer Biology 2019Quote: ... or 10 µL IM156 medium containing 10% dialyzed FBS and [13C]-glutamine (Cambridge Isotope Laboratories). Cells were washed twice with normal saline ...
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bioRxiv - Evolutionary Biology 2020Quote: ... (2) D4 L-Lysine (Cambridge Isotope Laboratories) for “medium” (M ...
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bioRxiv - Molecular Biology 2023Quote: ... and 2 g 13C-glucose (Cambridge isotope). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... uniformly 15 N- and 13 C-labeled proteins were produced by growing cell cultures in M9 minimal medium that contained 1 g/l 15 N-ammonium chloride and 2 g/l 13 C-D-glucose (Cambridge Isotope Laboratories, Inc.) as the sources of nitrogen and carbon ...
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bioRxiv - Cell Biology 2022Quote: ... The third plate was cultured in heavy SILAC media with the same composition except arginine and lysine were replaced by L-Arginine [13C6,D7,15N4]HCl (Arg-17) and L-Lysine [13C6,15N2]HCl (Lys-8) (Cambridge Isotope Laboratories). Cells were passaged and expanded in the corresponding SILAC media for 10 doublings.
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bioRxiv - Cell Biology 2022Quote: ... Metabolites were extracted with 1 mL 80% MeOH pre-cooled to −80 °C containing 10 nmol [D8]-valine as an internal standard (Cambridge Isotope Labs). Insoluble material was removed by centrifugation at 21,000 ×g for 15 min at 4 °C ...
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bioRxiv - Biochemistry 2019Quote: ... with 10 g/L glucose or 10 g/L 13C glucose (Cambridge Isotope Laboratories, CLM-1396-25). Expression was induced using 1 mM isopropylthio-β-D-galactoside (IPTG ...
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bioRxiv - Biophysics 2022Quote: ... then centrifuging and resuspending in M9 minimal medium containing 2 g/L unlabeled glucose and 1 g/L 15N-labeled ammonium acetate (Cambridge Isotope Labs, Tewksbury, MA) and induced with 1 mM IPTG ...
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bioRxiv - Biochemistry 2022Quote: ... 1 % P/S and isotope-labelled D3-15N-L-serine (1 mM unless indicated otherwise) and D4-L-alanine 2 mM (Cambridge Isotope Laboratories, MA, USA). Cells were grown further in the labelling media for 16 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... Dried samples were rehydrated with 2:1 methanol:water into which a mixture of isotopically labeled amino acids of known concentrations (Cambridge Isotope Laboratories, MSK-A2-1.2) was added ...
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bioRxiv - Cell Biology 2020Quote: ... 500 μl of each cell lysate sample was blown to dryness with N2 and reconstituted with 150 μL of an Acetonitrile/H2O (2:1) solution containing stable isotope-labeled internal standards: tyrosine-d2 and lactate-13C3 (Cambridge Isotope Lab, Tewksbury, MA). Centrifuged the cell lysate at 10,000 g for 20 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... [13C4]-2-oxoglutarate ([13C4]-2OG) (Cambridge Isotope Laboratories), was added during metabolite extraction ...
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bioRxiv - Biochemistry 2022Quote: ... 10 µM 13C1-ribitol (Cambridge Isotope Laboratories) was added to methanol as an internal standard during extraction ...
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bioRxiv - Biochemistry 2023Quote: ... 10 μM 13C1-ribitol (Cambridge Isotope Laboratories) was added to methanol as an internal standard during extraction ...
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bioRxiv - Plant Biology 2019Quote: ... Ca(NO3)2 × 4H2O in solid and liquid PpNO3 media was replaced by Ca(15NO3)2 × 4H2O (Cambridge Isotope Laboratories, England). To obtain fully labelled protonema tissue the protonema cultures were weekly sub-cultivated on fresh 15N labelled solid PpNO3 media for at least 4 months ...
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bioRxiv - Cell Biology 2023Quote: ... Washed cells were extracted with 50 µL extraction solvent composed of 20% MeOH in water and including stable isotope-labelled caffeine (13C3, 8 µM, Cambridge Isotope Laboratory) and deuterated succinic acid (d4 ...
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bioRxiv - Cell Biology 2024Quote: ... 13C6-Glucose or 13C3-pyruvate were added for either the full 48 hours or in the last 8 hours accompanied by a media change (Cambridge Isotope Laboratories). Cells were then washed with ice-cold PBS three times and metabolites extracted using an extraction solvent consisting of 50% methanol ...
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bioRxiv - Microbiology 2021Quote: ... The cosms were surface-irrigated at 7 am with 60 ml of either 19.85 mM 15NH4Cl (99% 15N, Cambridge Isotope Laboratories, Inc., MA, US, pH 5.47) or a 19.98 mM 15KNO3 (99% 15N ...
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bioRxiv - Molecular Biology 2022Quote: ... [2-13C]glycine (Cambridge Isotope Laboratories, CLM-136-PK). All cell culture experiments were seeded with a starting cell density of 100,000 cells per mL and cultured for 2 days in the specified culture media ...
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bioRxiv - Molecular Biology 2022Quote: ... [2-13C]serine (Cambridge Isotope Laboratories, CLM-2013-PK), [2-13C]glycine (Cambridge Isotope Laboratories ...
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bioRxiv - Microbiology 2023Quote: ... Ethyl [U-13C]2-hydroxybutyrate (Cambridge Isotope Laboratories, Inc.) approximated 2-hydroxybutyrate and 2-aminobutyrate signals ...
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bioRxiv - Molecular Biology 2024Quote: ... [15N]5-2-deoxyadenosine was obtained from Cambridge Isotope Laboratories ...
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bioRxiv - Biochemistry 2022Quote: Amino acids were quantified from 10 µL plasma precipitated with 180 µl ice-cold methanol containing 1 nmol of stable isotope labelled amino acids (Cambridge Isotope Laboratories, MSK-A2-1.2). Samples were incubated at -20°C for 30 min followed by centrifugation at 4°C (14,000g ...
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bioRxiv - Developmental Biology 2019Quote: ... supplemented with 5 μl of heavy Lysine (Lys-8; stock: 30 mg/ml) (L-Lysine: 2HCl, U-13C6, 99%; U-15N2, 99%, Cambridge Isotope Laboratories, Inc.). The culture was incubated at 30°C for 24 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... and DMEM without glucose and pyruvate supplemented with 10% dialyzed FBS and 10 mM U-13C-glucose (Cambridge Isotope Laboratories) was added for 24 hours prior to metabolite extraction.
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bioRxiv - Biochemistry 2019Quote: ... deuterated glucose (Cambridge Isotope Laboratories, DLM-2062-10), and deuterium oxide (Cambridge Isotope Laboratories ...
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bioRxiv - Biochemistry 2023Quote: ... cells were cultured in RPMI medium containing 10% dialyzed FBS and 10 µM [15N4]hypoxanthine (Cambridge Isotope Laboratories, NLM-8500-0.1). For all drug-treated samples ...
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bioRxiv - Cancer Biology 2022Quote: ... The following day cells were washed twice with PBS and then incubated with 8mL for 8 or 24h in TIFM with 13C5-citrulline (Cambridge Isotope Laboratories, CLM-8653) added at TIFM concentrations ...
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bioRxiv - Biochemistry 2024Quote: ... and 2 g/L U-13C6-Glucose (Cambridge Isotope Laboratories) as the sole nitrogen and carbon sources ...
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bioRxiv - Molecular Biology 2021Quote: ... and 10 mM C13 labeled glucose (Cambridge Isotope Laboratories). 24 hours later the cells were washed twice with ammonium acetate (ThermoFisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... and 10 mM 13C-labeled glucose (Cambridge Isotope Laboratories). Cells were washed with ice-cold saline and snap frozen on dry-ice and stored at −80°C.
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bioRxiv - Microbiology 2019Quote: ... 15N-labeled amino acids (10 mM; Cambridge Isotope Laboratories), or keto acids (20 mM ...
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bioRxiv - Biochemistry 2021Quote: D-Glucose (Cambridge Isotope Laboratories, Cat. # DLM-2062-10)
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bioRxiv - Cancer Biology 2023Quote: ... Alternatively post 48 hr LNCaP monoclonal CRISPR KOs were washed with PBS and media was replaced with DMEM (Dulbecco’s Modified Eagle’s Medium) medium containing 10 mM [U-13C]-glucose (Cambridge Isotope laboratories: #CLM-1396-10), 2 mM unlabeled glutamine and 10% dialyzed FBS (in triplicate) ...
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bioRxiv - Bioengineering 2023Quote: ... supplemented with 2 g / L 13C6–D-glucose (Cambridge Isotope Laboratories) and sodium bicarbonate (Sigma Aldrich) ...
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bioRxiv - Biochemistry 2022Quote: ... and switched from DMEM + 10% FBS to DMEM + 10% dialyzed FBS (dFBS, GeminiBio #100-108) + 1mM 13C2 - acetate (Cambridge Isotope Laboratories #CLM-440-0) for 18 hours ...
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bioRxiv - Biophysics 2022Quote: ... 15N NH4Cl (Cambridge Isotope Laboratories; Cat. No. NLM-467-10) was used (1g/L of the media ...
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bioRxiv - Microbiology 2024Quote: ... and 10 mM U-13C6-Glc (Cambridge Isotope Laboratories, CLM-1396) or U-13C6-glucosamine (Cambridge Isotope Laboratories ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated in RPMI containing 2 mM 13C5,15N2-glutamine (Cambridge Isotope Labs) as the sole glutamine source for 2 hours before metabolites were extracted and quantified as described above with the exception that no internal standards were added to the extraction buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.05 U Phosphodiesterase I (Sigma # P3243)] and [15N]5-2’-deoxyadenosine ([15N]-dA) (Cambridge Isotope Laboratories # NLM-3895-25) as the internal standard to account for variations in sample handling and mass spectrometer response59 ...
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bioRxiv - Biophysics 2020Quote: ... and 10% DMSO-d6 (v/v) (Cambridge Isotope Laboratories, Tewksbury, MA, USA). DMSO-d6 served as the deuterium lock solvent and prevented freezing at low temperatures ...
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bioRxiv - Biochemistry 2021Quote: ... and 10% DMSO-d6 (v/v) (Cambridge Isotope Laboratories, Tewksbury, MA, USA). DMSO-d6 served as the deuterium lock solvent and prevented freezing at low temperatures ...
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bioRxiv - Biophysics 2022Quote: ... 10% D2O (v/v) (Cambridge Isotope Laboratories; Cat. No. DLM-4-25) was added to the sample for the spectrometer deuterium lock ...
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bioRxiv - Biochemistry 2023Quote: ... 10 mL Bioexpress Cell Growth Media (Cambridge Isotope Laboratories, Tewksbury, MA, USA), 5 mL Gibco™ MEM Vitamin Solution (100x) ...
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bioRxiv - Cancer Biology 2019Quote: ... or by HBSS solution supplemented with 2 mM 15N-glutamic acid (Cambridge Isotope Laboratories), 5 mM glucose ...
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bioRxiv - Cell Biology 2019Quote: ... cells were cultured in 10 mM [U-13C6]glucose (Cambridge Isotope Laboratories, Inc.) containing either 1000 µM serine/glycine or no serine/glycine for 48 h prior to extraction ...
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bioRxiv - Microbiology 2024Quote: ... 10 µL of 50 mM methyl-d3 pentadecanoic acid (98%, Cambridge Isotope Laboratories) or 7,7,8,8-d4 palmitic acid (98% ...
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bioRxiv - Immunology 2019Quote: Cells were cultured in glucose-free media with 2 mM 13C6-glucose (Cambridge Isotope Laboratories) or [13C5]-ribose-inosine (Omicron Biochemicals ...