Labshake search
Citations for Cambridge Isotope Labs :
151 - 200 of 206 citations for 6 TERT BUTYLCHROMONE 2 CARBOXYLIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... cells were washed twice with ice-cold 0.9% NaCl and polar metabolites extracted in ice cold 80% LC/MS grade methanol containing 15N and 13C fully-labeled amino acid standards (Cambridge Isotope Laboratories #MSK-A2-1.2). Extracts were vigorously shaken by vortex for 10 minutes at 4°C and spun at 14,000 rpm at 4°C for 10 minutes to remove insoluble cell debris ...
-
bioRxiv - Genomics 2023Quote: ... followed by metabolite extraction in ice cold 80:20 LC/MS grade methanol: water buffer containing 15N and 13C fully-labeled amino acid standards (Cambridge Isotope Laboratories #MSK-A2-1.2). Samples were vortexed for 10 minutes at 4°C and then centrifuged at maximum speed (> 14,000g ...
-
Multi-omics characterization of partial chemical reprogramming reveals evidence of cell rejuvenationbioRxiv - Molecular Biology 2023Quote: ... Protein was precipitated from 10 µl of sample by addition of nine volumes of 74.9:24.9:0.2 v/v/v acetonitrile/methanol/formic acid containing internal standards (valine-d8, Sigma-Aldrich; St. Louis, MO; and phenylalanine-d8, Cambridge Isotope Laboratories; Andover, MA). Precipitated material was cleared by centrifugation and the supernatant was injected directly onto a 150 x 2 mm 3 µm Atlantis HILIC column (Waters ...
-
bioRxiv - Synthetic Biology 2021Quote: ... a 80:20 mixture of 6-13C glucose:U-13C6 glucose or a 80:20 mixture of 1-13C glucose:U-13C6 glucose (Cambridge Isotope, MA and Omicron Biochemicals, IN). Cultures used as internal standards for metabolomic measurements were grown in two subsequent M9 minimal mediums cultures containing 100% U-13C6.
-
bioRxiv - Cell Biology 2020Quote: ... cells were incubated in RPMI containing 2 mM 13C5,15N2-glutamine (Cambridge Isotope Labs) as the sole glutamine source for 2 hours before metabolites were extracted and quantified as described above with the exception that no internal standards were added to the extraction buffer ...
-
bioRxiv - Molecular Biology 2019Quote: ... 0.05 U Phosphodiesterase I (Sigma # P3243)] and [15N]5-2’-deoxyadenosine ([15N]-dA) (Cambridge Isotope Laboratories # NLM-3895-25) as the internal standard to account for variations in sample handling and mass spectrometer response59 ...
-
bioRxiv - Biochemistry 2023Quote: ... supplemented with 2 mM [amide-15N]glutamine (Cambridge Isotope Laboratories, NLM-557-1) and 10% dialyzed FBS ...
-
bioRxiv - Biophysics 2024Quote: ... and 2 g L-1 of [15N] ammonium chloride (Cambridge Isotope Laboratories, CIL) as the sole carbon and nitrogen source ...
-
bioRxiv - Biophysics 2023Quote: ... They were then resuspended in 500 ml of M9 media supplemented with 100 mg of a desired 15N-labeled or 13C-labeled L-amino acid (Cambridge Isotope Labs, Inc. or Sigma-Aldrich, Inc.) as well as the remaining 19 unlabeled amino acids ...
-
bioRxiv - Systems Biology 2021Quote: ... 13C-[6C]-labeled 2-deoxy-D-glucose (Cambridge Isotope Laboratories, Inc., 12 Ci/mmol); [1-3H] 2-Deoxy-D-glucose (ViTrax Radiochemicals) ...
-
bioRxiv - Immunology 2022Quote: ... of the samples was used to scale the metabolite extraction to a ratio of 1×106 cells / 1 mL extraction solution [80% LCMS grade methanol (aq) with 500nM labelled amino acid internal standard (Cambridge Isotope Laboratories, Inc., Cat No. MSK-A2-1.2)] ...
-
bioRxiv - Immunology 2019Quote: Cells were cultured in glucose-free media with 2 mM 13C6-glucose (Cambridge Isotope Laboratories) or [13C5]-ribose-inosine (Omicron Biochemicals ...
-
bioRxiv - Cell Biology 2023Quote: ... Mice were then injected with [U13C] succinate (10 mg/kg, 2 min) (Cambridge Isotope Laboratories) via intravenous tail vein ...
-
bioRxiv - Cell Biology 2022Quote: ... Labelled media refers to EMM with 2% D-Glucose (U-13C6) (Cambridge Isotope Laboratories, Inc.). Once cells came into contact with labelled media ...
-
bioRxiv - Biophysics 2023Quote: ... in dry acetonitrile (10 ml) was added 2-bromoethanol-1,1,2,2-d4 (240 μl, Cambridge Isotope Laboratories) under argon ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... a 2 mM solution of isotopically labeled sodium bicarbonate (NaH13CO3; Cambridge Isotope Laboratories Inc., Tewksbury, MA) was added to a batch culture of R ...
-
bioRxiv - Biophysics 2021Quote: ... 2 g/l of U-[2H,13C] D-glucose and 1 g/l 15NH4Cl (Cambridge Isotope Laboratories). For production of U-[2H ...
-
bioRxiv - Microbiology 2023Quote: ... Fractions 1–2 were prepared in 100% deuterated H2O (D2O; Cambridge Isotope Laboratories; referenced at 4.39 ppm) and Fractions 3–4 in 10% CD3OD ...
-
bioRxiv - Molecular Biology 2021Quote: ... and proteins were produced in the M9 media supplemented with 2 g/L [15N]NH4Cl (Cambridge Isotope Laboratories) and 3 g/L d-[13C6]glucose (Cambridge Isotope Laboratories) ...
-
bioRxiv - Biophysics 2022Quote: ... and 2 g/L U-[2H] glucose (Sigma-Aldrich, St. Louis, MO/Cambridge Isotope Laboratories, Inc., Tewksbury, MA). Cells were grown up to an OD600 of 0.65 at 37 °C ...
-
bioRxiv - Cell Biology 2022Quote: ... 50 µl of 2× kinase buffer containing 80 µM O18 ATP (OLM-7858-PK, Cambridge Isotope laboratories, Inc) and 1 µg of bacterial purified human PIP4Kα-GST was added and the reaction was incubated at 30 °C for 1 hour ...
-
bioRxiv - Plant Biology 2022Quote: ... and 2 ng of [13C6]-IAA was added as an internal standard (Cambridge Isotope Laboratories, Andover, MA, USA). The sample was extracted for 2h at 4°C under continuous shaking ...
-
bioRxiv - Plant Biology 2023Quote: RG-II (1-2 mg) was dissolved in D2O (0.2 mL, 99.9%; Cambridge Isotope Laboratories, Tewksbury, MA, USA) and placed in a 3 mm NMR tube 45 ...
-
bioRxiv - Microbiology 2019Quote: ... we replaced the glucose in our standard HIGG media with deuterated 2-D glucose (Cambridge Isotope Laboratories, DLM-1271). During the isomerization of glucose to fructose ...
-
bioRxiv - Cancer Biology 2022Quote: ... and re-plated in glutamine free media that was supplemented with either 2 mM 15N-glutamine (Cambridge isotope laboratories) or 2 mM GlutaMAX (represents the 0 hours timepoint of 15N-glutamine tracing) ...
-
bioRxiv - Cancer Biology 2022Quote: ... the dried pellet was dissolved in 550 µl D2O containing 0.01 mg/ml Sodium 2,2-Dimethyl-2-Silapentane-5-Sulfonate (DSS; Cambridge Isotope). The samples were then vortexed and centrifuged at 16000 rpm for 12 min ...
-
bioRxiv - Plant Biology 2023Quote: ... The plant materials were extracted with the extraction buffer containing 2 μM labeled Met (13C, 15N, Cambridge Isotope Laboratories), 10 μM DL-dithiothreitol (DTT ...
-
bioRxiv - Microbiology 2023Quote: ... glycine (2-13C, 99%; 15N, 98%+), L-phenylalanine (D8, 98%), or tyramine:HCl (1,1,2,2-D4, 98%) (Cambridge Isotope Laboratories, Inc.) was added to BHIS containing 0.1% (w/v ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Samples were thawed on ice and phthalates were extracted from a 10 μL aliquot via vigorous vortexing for 30 min at 4°C in the presence of 5:3:2 methanol:acetonitrile:water (240 μL) containing an isotope labeled standard (1 μM DEHP ring-1,2-13C2, Cambridge Isotope Laboratories). Extraction supernatants were clarified via centrifugation at 18,000 rpm for 10 min at 4°C and analyzed immediately by ultra high-pressure liquid chromatography coupled to mass spectrometry (UHPLC-MS) ...
-
bioRxiv - Microbiology 2019Quote: ... [2-13C] glycerol (99%) and an equimolar mixture of [U-13C3] glycerol (99%) (Cambridge Isotope Laboratories, Inc., Andover, MA, USA) and naturally labelled glycerol ...
-
bioRxiv - Biochemistry 2021Quote: ... containing 0.11 mM of the internal standard DSS (sodium 2,2-dimethyl-2-silapentane-5-sulfonate, D6, 98%; Cambridge Isotope Laboratories) at pH 7.0 and vortex mixed for <1 min prior to transfer into 1.7 mm SampleJet NMR tubes ...
-
bioRxiv - Plant Biology 2022Quote: ... 2-13C-acetate and U-13C-acetate (13C isotopic purity of 99%, sodium salts from Cambridge Isotope Laboratories (Eurisotop, France); Figure 1) ...
-
bioRxiv - Immunology 2023Quote: 2×106 BMDCs were treated with 1mM uniformly labeled 13C lactate (L-LA*) (CLM-1579-N-0.1MG, Cambridge Isotope Laboratories), in the presence or absence of LPS (100ng/mL) ...
-
bioRxiv - Molecular Biology 2024Quote: EGM-2 media was replaced with DMEM media containing D-Glucose (U-13C6, CLM-1396-PK, Cambridge Isotope Laboratories Inc.) 3 hours prior to collection ...
-
bioRxiv - Cancer Biology 2021Quote: ... PaTu-8902 and MIAPaCa-2 iDox-shGOT2.1 cells were cultured in media containing 1 mM unlabelled glucose and 1 mM U13C-Pyruvate (Cambridge Isotope Laboratories) for 16 hours.
-
bioRxiv - Systems Biology 2022Quote: ... in a 100 mM sodium phosphate buffered solution with 0.11 mM sodium 2,2-dimethyl-2-silapentane-5-sulfonate (DSS-D6; 98%; Cambridge Isotope Laboratories, Inc.). Once dry ...
-
bioRxiv - Plant Biology 2019Quote: ... or 15N MS medium (1/2 MS without nitrogen source [PhytoTechnology Laboratories], 15NH415NO3 [0.5 g/L, NLM-390-1, Cambridge Isotope Laboratory] ...
-
bioRxiv - Biochemistry 2023Quote: ... The 6% site-specific 13C/15N isotopically labelled oligonucleotide were synthesized on K&A H8 synthesizer using the 2’-deoxyadenosine phosphoramidite (98% 15N) purchased from Cambridge Isotope Laboratories (USA) ...
-
bioRxiv - Cancer Biology 2023Quote: ... U-[13C]-Q labelling media consisted of DMEM supplemented with 2 mM L-glutamine (U-13C5; CLM-1822; Cambridge Isotope Laboratories), 10 mM glucose ...
-
bioRxiv - Biophysics 2023Quote: ... except that yeast cells were grown in SD-CSM media made with 2% (w/v) protonated 13C-depleted glucose (99.9% 12C, Cambridge Isotope Labs) as the carbon source ...
-
bioRxiv - Cancer Biology 2021Quote: ... the cells were washed with PBS and fed 1 ml of culture medium supplemented with 2 mM [U-13C]glutamine (CLM-1822, Cambridge Isotope Laboratories) or 12.5 mM [1,2-13C]glucose (CLM-504-1 ...
-
bioRxiv - Developmental Biology 2020Quote: Larvae collected at 0 hpe were separated into four 1.5 mL tubes each containing 10 individuals and incubated with 1.2 mL FSW + 1.2 μl 10 mM 13C-arginine (Lot No. PR-21659, Catalog No. CLM-2070-0, Cambridge Isotope Laboratories, Inc.). From each tube ...
-
bioRxiv - Immunology 2021Quote: CD138+ PCs were sorted on day 4 of in vitro differentiation and grown for 18 hrs in glucose free 1640 RPMI (Thermo) with dialyzed FBS supplemented with 2 g/L [U-13C6] glucose (Cambridge Isotope Laboratories). Metabolites were extracted and measured using Ultra High Performance Liquid Chromatography Mass Spectrometry (UHPLC-MS) ...
-
bioRxiv - Plant Biology 2020Quote: ... 200 μL of 65% isopropanol /35% 0.2 M pH 7.0 imidazole buffer (Chen et al. 1988) was then added with [13C6] IAA internal standard (Cambridge Isotope Laboratories Inc.), and samples were extracted on ice for 1 h ...
-
bioRxiv - Cancer Biology 2022Quote: ... and re-plated for 12 hours in glutamine free media that was supplemented with either 2 mM 15N-glutamine (Cambridge isotope laboratories) or 2 mM GlutaMAX (Gibco Inc. ...
-
bioRxiv - Biophysics 2020Quote: ... Cells were pelleted and transferred to M9 media containing 1 g of 15N-ammonium chloride and 2 g of 13C uniformly label glucose (Cambridge Isotope Laboratories). After transfer ...
-
bioRxiv - Cancer Biology 2022Quote: ... 3×106 cells/well were seeded in a 6-well plate and cultured in 2 mL assay media supplemented with 10% dialyzed FBS (Wisent) and either 25 mM U-[13C]-glucose (Cambridge Isotope Laboratories) and 4 mM glutamine (Sigma ...
-
bioRxiv - Microbiology 2021Quote: ... uniformly 15 N- and 13 C-labeled proteins were produced by growing cell cultures in M9 minimal medium that contained 1 g/l 15 N-ammonium chloride and 2 g/l 13 C-D-glucose (Cambridge Isotope Laboratories, Inc.) as the sources of nitrogen and carbon ...
-
bioRxiv - Biophysics 2022Quote: ... then centrifuging and resuspending in M9 minimal medium containing 2 g/L unlabeled glucose and 1 g/L 15N-labeled ammonium acetate (Cambridge Isotope Labs, Tewksbury, MA) and induced with 1 mM IPTG ...
-
bioRxiv - Biochemistry 2022Quote: ... 1 % P/S and isotope-labelled D3-15N-L-serine (1 mM unless indicated otherwise) and D4-L-alanine 2 mM (Cambridge Isotope Laboratories, MA, USA). Cells were grown further in the labelling media for 16 hours ...