Labshake search
Citations for Cambridge Isotope Labs :
251 - 300 of 363 citations for 6 Heptenoic acid 7 2 cyclopropyl 4 4 fluorophenyl 3 quinolinyl 3 5 dihydroxy calcium salt 1 1 3R 5S 6E since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... SRM transitions were detailed in the TraceFinder software and then verified with the Metabolomics Amino Acid Mix Standard (Cambridge Isotope Laboratories) for unique transitions ...
-
bioRxiv - Physiology 2020Quote: ... on Day 1 each participant ingested 150 mL of Deuterium Oxide (D2O) (70 atom %, Cambridge Isotope Laboratories) as previously described (63) ...
-
bioRxiv - Biochemistry 2020Quote: ... or ‘heavy’ 13C615N2-Lysine (Silantes; 211604102) and 13C615N4-Arginine (Cambridge Isotope Labs Inc; CNLM-539-H-1). Cells were cultured in SILAC ‘light’ or ‘heavy’ media for two weeks prior to harvesting by centrifugation (800g ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cell were depleted of methionine for 1h and then 1 mM L-azidohomoalanine (AHA, Cambridge Isotope Laboratories) was added to the media with a dose of CDCexo (103 EV/ cell ...
-
bioRxiv - Biochemistry 2024Quote: ... and 2 g/L U-13C6-Glucose (Cambridge Isotope Laboratories) as the sole nitrogen and carbon sources ...
-
bioRxiv - Cell Biology 2020Quote: ... L amino acids were substituted for the same concentrations of heavy isotope–labeled (H) amino acids 13C6-15N2 L-Lys:2HCl and 13C6-15N4 L-Arg (Cambridge Isotope Laboratories). For immature dendritic cells (Dc ...
-
bioRxiv - Cell Biology 2020Quote: Cells were washed twice with ice cold PBS and extracted on dry-ice in 0.8 mL 80% methanol containing 500 nM internal standards (Metabolomics Amino Acid Mix Standard: Cambridge Isotope Laboratories, Inc.). Cell extracts were collected using a cell scraper and transferred to a microcentrifuge tube ...
-
bioRxiv - Cell Biology 2022Quote: ... Polar metabolites were extracted in 80 % methanol containing 15N and 13C fully-labeled amino acid standards (MSK-A2-1.2, Cambridge Isotope Laboratories, Inc). Extracts were shaken for 10 min with a vortexer ...
-
bioRxiv - Cell Biology 2021Quote: ... and 500 μl of ice-cold 80% methanol in water with 250nM 13C/15N labeled amino acid standards (MSK-A2-1.2: Cambridge Isotope Laboratories, Inc.) was added to each well ...
-
bioRxiv - Cell Biology 2021Quote: ... which were replaced with either unlabelled amino-acids (Lys0 and Arg0) or stable isotopic forms 13C6 15N2-lysine (Lys8) and 13C6 15N4-arginine (Arg10) (Cambridge Isotope Laboratories). The medium was supplemented with 10% dialyzed FBS using previous published methods (Golebiowski et al ...
-
bioRxiv - Microbiology 2021Quote: ... 2 mL of LC-MS grade water containing 0.1 μL of MSK-A2-1.2 stable isotope-labeled amino acid standards (Cambridge Isotope Laboratories, Tewksbury, MA) was added before vortexing for 1 minute ...
-
bioRxiv - Biochemistry 2021Quote: ... then 10% of bead volume was lysed with 1% Triton buffer for protein extracts and the remaining 90% was extracted in 80% methanol containing heavy labeled amino acid standards (Cambridge Isotope Labs) on a rotator shaker for 10□min at 4□°C.
-
bioRxiv - Cell Biology 2023Quote: ... and either “Heavy” amino acids (50 μg/ml 13C6, 15N4 Arginine HCl and 100 μg/ml 13C6, 15N2 Lysine 2HCl; Cambridge Isotope Laboratories), or the equivalent “Light” amino acids ...
-
bioRxiv - Cancer Biology 2023Quote: ... Media was aspirated from cells and then washed rapidly in ice cold bank saline followed by addition of 500 mL ice-cold 80% methanol in water containing 500 nM each of 17 isotopically labeled 13C/15N amino acids (Cambridge Isotope Laboratories). Alternatively ...
-
Sex-specific fear acquisition following early life stress is linked to amygdala glutamate metabolismbioRxiv - Animal Behavior and Cognition 2024Quote: ... U-13C15N-labeled amino acids at a final concentration of 0.25 µM (prepared as 2.5 mM in 0.1 N HCl, Cambridge Isotope Laboratories, MSK-A2-1.2), citric acid d4 at 0.02 µg/mL (dissolved at 100 µg/mL in H2O ...
-
bioRxiv - Cancer Biology 2021Quote: ... 7.0 × 105 cells were plated onto 6 well plates for next day pulse-labeling with either 25 mM [1,2-13C] glucose (Cambridge Isotope Laboratories). Isotope labeling experiments were performed for 4 hours and extra plates were included for cell counts ...
-
bioRxiv - Microbiology 2019Quote: ... or 1 mM unlabeled methanol and 10 mM13C-labeled sodium bicarbonate (13C-labeled substrates provided by Cambridge Isotope Laboratories ...
-
bioRxiv - Cancer Biology 2021Quote: ... received one dose of 1 mg/kg creatine-(methyl-d3) (DLM-1302-0.25, Cambridge Isotope Laboratories, Tewksbury, MA) by i.p ...
-
bioRxiv - Developmental Biology 2022Quote: ... flies were fed for 12hrs a mixture of 0.5M 13C-glucose (Cambridge Isotope Laboratories, Inc #CLM-1396-1) and 0.5M unlabeled glucose (Sigma #G5400) ...
-
bioRxiv - Cancer Biology 2023Quote: ... 13C-labeling was performed in 10 mL of DMEM containing 20 mM [1- 13C] of glucose (Cambridge Isotope Laboratories ...
-
bioRxiv - Biochemistry 2023Quote: ... The cells were scrapped in 50% methanol and 100 ug D4 alanine (Cambridge isotope lab DLM-250-1) and centrifuged at 13000 RPM for 5 minutes at room temperature ...
-
bioRxiv - Cell Biology 2024Quote: ... cells were incubated with media containing the designated tracer γ-15N-glutamine (Cambridge Isotope Laboratories: NLM-557-1), or U-13C-glucose (Cambridge Isotope Laboratories ...
-
bioRxiv - Systems Biology 2023Quote: ... T87 cells were cultured in 15N-enriched NT-1 liquid medium containing K15NO3 and 15NH415NO3 (Cambridge Isotope Labs), under identical culture conditions ...
-
bioRxiv - Cancer Biology 2020Quote: ... 10μL of media was mixed with 10μL of a mixture of isotopically labeled metabolites and amino acids of known concentrations (Cambridge Isotope Laboratories, Tewksbury, MA) and extracted with 600μL ice-cold HPLC grade methanol ...
-
bioRxiv - Neuroscience 2020Quote: F2 SILAM wide type female mice were purchased from Cambridge Isotope Laboratories and then crossed with regular C57BL6 wild type males by fed with stable isotope labeling using amino acids in cell culture (SILAC) L-lysine (13C6) feed (Cambridge Isotope Laboratories, Inc). After 7 days ...
-
bioRxiv - Microbiology 2023Quote: ... were prepared via protein precipitation with the addition of 9 volumes of 74.9:24.9:0.2 v/v/v acetonitrile/methanol/formic acid containing stable isotope-labeled internal standards (valine-d8, Sigma-Aldrich; and phenylalanine-d8, Cambridge Isotope Laboratories). The samples were centrifuged (10 minutes ...
-
bioRxiv - Cancer Biology 2023Quote: ... The tissue powder was then weighed into pre-chilled tubes and extracted with methanol (containing 500 nM each of 17 isotopically labeled 13C/15N amino acids (Cambridge Isotope Laboratories, Inc.)) ...
-
bioRxiv - Bioengineering 2023Quote: ... supplemented with 2 g / L 13C6–D-glucose (Cambridge Isotope Laboratories) and sodium bicarbonate (Sigma Aldrich) ...
-
bioRxiv - Biochemistry 2021Quote: ... 5 mL 15N Bioexpress Cell Growth Medium (Cambridge Isotope Laboratories, Tewksbury, MA, USA), 5 mL of Gibco MEM Vitamin Solution (100x) ...
-
bioRxiv - Cancer Biology 2019Quote: ... cells were incubated with either 13C6-glucose (CLM-1396-5, Cambridge Isotope Laboratories) or 15N2-glutamine (NLM-1328-0.25 ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were blocked with 50mM ammonium chloride (Cambridge Isotope Laboratories, NLM-467-5), 0.01% bovine serum albumin (BSA ...
-
bioRxiv - Animal Behavior and Cognition 2022Quote: ... were diluted using 90 µL of 74.9:24.9:0.2 v/v/v acetonitrile/methanol/formic acid containing stable isotope-labeled internal standards (valine-d8, Isotec; and phenylalanine-d8, Cambridge Isotope Laboratories; Andover, MA). The samples were centrifuged (10 min ...
-
bioRxiv - Physiology 2021Quote: ... continuous infusion of stable amino acid isotope [13C6]-phenylalanine (L-phenylalanine, ring-13C6, 99%, CLM-1055-MPT; Cambridge Isotope Laboratories, Inc., Tewksbury, MA, USA) was used for measurement of amino acid kinetics across the limb and incorporation of labeled phenylalanine into muscle as previously described [20] ...
-
bioRxiv - Biophysics 2021Quote: ... 13C,15N-labelled 24x’ was expressed using a 1/100 dilution of Bioexpress media (Cambridge Isotope Labs, Tewksbury, MA) in labelled M9 media (51) ...
-
bioRxiv - Cancer Biology 2020Quote: ... cells were incubated for 24 h in L-L or H-H medium supplemented with either 0.8mM 15N-ammonium chloride (99% enrichment, NLM-467-1) or 0.4mM 15N-leucine (98% enrichment, NLM-142-0.5) purchased from Cambridge Isotope Laboratories ...
-
bioRxiv - Biochemistry 2023Quote: ... coli BL21(DE3)pLysS cells in standard M9 minimal medium using 1 g/l 15N NH4Cl (Cambridge Isotope Laboratories) as a sole nitrogen source ...
-
Ralstonia solanacearum alters root developmental programs in auxin-dependent and independent mannersbioRxiv - Plant Biology 2023Quote: ... A total of 0.5 mL 70% methanol containing 1 ng [13C6]-IAA (CLM-1896-0.01, Cambridge Isotope Laboratories, USA), as an internal standard ...
-
bioRxiv - Immunology 2024Quote: ... and replated in media containing the heavy isotope of interest (13C6-glucose (Cambridge Isotope Laboratories, Inc., CLM-1396-1) and 13C5-glutamine (CK Isotopes ...
-
bioRxiv - Biochemistry 2023Quote: ... but glucose was omitted and instead 5 mM 13C3-U-glycerol (Cambridge Isotope Laboratories) was supplied ...
-
bioRxiv - Microbiology 2022Quote: ... supplemented with 10 mM 13C6-glucose (Cambridge Isotope Laboratories, CLM-1396-2) and dialyzed FBS (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2022Quote: ... were resuspended in 300 μL of LC/MS grade methanol containing a mixture of 17 isotope-labeled amino acids (200 nM, Cambridge Isotope Laboratories, MSK-A2-1.2). To this solution ...
-
bioRxiv - Cell Biology 2022Quote: ... Polar metabolites were extracted in ice cold 80% LC/MS grade methanol containing 15N and 13C fully-labeled amino acid standards (Cambridge Isotope Laboratories #MSK-A2-1.2). Tissues were homogenized by Bead Ruptor 24 (Omni International ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were washed twice with ice-cold 0.9% NaCl and polar metabolites extracted in ice cold 80% LC/MS grade methanol containing 15N and 13C fully-labeled amino acid standards (Cambridge Isotope Laboratories #MSK-A2-1.2). Extracts were vigorously shaken by vortex for 10 minutes at 4°C and spun at 14,000 rpm at 4°C for 10 minutes to remove insoluble cell debris ...
-
bioRxiv - Genomics 2023Quote: ... followed by metabolite extraction in ice cold 80:20 LC/MS grade methanol: water buffer containing 15N and 13C fully-labeled amino acid standards (Cambridge Isotope Laboratories #MSK-A2-1.2). Samples were vortexed for 10 minutes at 4°C and then centrifuged at maximum speed (> 14,000g ...
-
Multi-omics characterization of partial chemical reprogramming reveals evidence of cell rejuvenationbioRxiv - Molecular Biology 2023Quote: ... Protein was precipitated from 10 µl of sample by addition of nine volumes of 74.9:24.9:0.2 v/v/v acetonitrile/methanol/formic acid containing internal standards (valine-d8, Sigma-Aldrich; St. Louis, MO; and phenylalanine-d8, Cambridge Isotope Laboratories; Andover, MA). Precipitated material was cleared by centrifugation and the supernatant was injected directly onto a 150 x 2 mm 3 µm Atlantis HILIC column (Waters ...
-
bioRxiv - Microbiology 2021Quote: 13C-labeled cystine (1,1’-13C2-L-cystine) was prepared from 1-13C-L-cysteine (Cambridge Isotope Labs, CLM-3852-0.5) via a modification of the procedure of Hill ...
-
bioRxiv - Microbiology 2020Quote: ... the experiment was additionally repeated in media made from 18O enriched water (OLM-240-10-1, Cambridge Isotope Laboratories, Inc.) at a final δ18Owater of approximately +100‰.
-
bioRxiv - Biochemistry 2022Quote: ... Samples were labeled with 40 mM isotopically distinct dimethyl formaldehyde (light, CH2O, for the parental RPE-1 cells (Cambridge Isotope Laboratories ...
-
bioRxiv - Systems Biology 2023Quote: ... cells were grown in SCD medium for 24hrs and then pulsed with 1% 13C2-acetate (Cambridge Isotope Laboratories, CLM-440). Metabolites were extracted after 30 minutes of labelled acetate addition ...
-
bioRxiv - Plant Biology 2023Quote: ... The following isotope-labelled standards were added at 1 pmol per sample: 13C6-IAA (Cambridge Isotope Laboratories, Tewksbury, MA, USA); 2H4-SA (Sigma-Aldrich ...