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Citations for Cambridge Isotope Labs :
151 - 200 of 231 citations for 3 Phenoxybenzoic Acid Unlabeled 100 Ug Ml In Acetonitrile since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% FBS and heavy isotope-labeled amino acids (L-Lysine 13C6, L-Arginine 13C6) (CLM-2247-H-1, CLM-2265-H-1, Cambridge Isotope Laboratories). DAC-treated cells grown in the regular medium were considered as SILAC light counterparts.
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bioRxiv - Cell Biology 2021Quote: ... and 500 μl of ice-cold 80% methanol in water with 250nM 13C/15N labeled amino acid standards (MSK-A2-1.2: Cambridge Isotope Laboratories, Inc.) was added to each well ...
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bioRxiv - Biochemistry 2020Quote: ... and 0.798 mM L-(13C6,15N2)-lysine (Lys“8”)] or “medium” amino acids [0.398 mM L-(15N4)-arginine (Arg“4”) and 0.798 mM L-(D4)-lysine (Lys“4”)] (Cambridge Isotope Laboratories, Tewksbury, USA), and incubated for 2 h ...
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bioRxiv - Cell Biology 2021Quote: ... which were replaced with either unlabelled amino-acids (Lys0 and Arg0) or stable isotopic forms 13C6 15N2-lysine (Lys8) and 13C6 15N4-arginine (Arg10) (Cambridge Isotope Laboratories). The medium was supplemented with 10% dialyzed FBS using previous published methods (Golebiowski et al ...
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bioRxiv - Microbiology 2021Quote: ... 2 mL of LC-MS grade water containing 0.1 μL of MSK-A2-1.2 stable isotope-labeled amino acid standards (Cambridge Isotope Laboratories, Tewksbury, MA) was added before vortexing for 1 minute ...
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bioRxiv - Biochemistry 2022Quote: ... and 0.798 mM L-(13C6,15N2)-lysine (Lys”8”)] or “medium-heavy” amino acids [0.398 mM L-(13C6)-arginine (Arg”6”) and 0.798 mM L-(D4)-lysine (Lys”4”)] (Cambridge Isotope Laboratories, Tewksbury, USA). For chloramphenicol (CAP ...
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bioRxiv - Biochemistry 2021Quote: ... then 10% of bead volume was lysed with 1% Triton buffer for protein extracts and the remaining 90% was extracted in 80% methanol containing heavy labeled amino acid standards (Cambridge Isotope Labs) on a rotator shaker for 10□min at 4□°C.
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bioRxiv - Neuroscience 2023Quote: ... each rat was fed 1g of a pancake that contained 4 µl of [13C]-octanoic acid (Cambridge Isotope Laboratories, Inc, Tewksbury MA). After visually confirming the rats had eaten the entire pancake (in less than 5min or they were omitted from the study) ...
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bioRxiv - Microbiology 2023Quote: ... or medium (84 mg L−1 13C6 L-arginine and 146 mg L−1 2H4 L-lysine) SILAC amino acids (Cambridge Isotope Laboratories). Medium SILAC labeled HeLa or HeLa S3 cells were used as spike-in control ...
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Sex-specific fear acquisition following early life stress is linked to amygdala glutamate metabolismbioRxiv - Animal Behavior and Cognition 2024Quote: ... U-13C15N-labeled amino acids at a final concentration of 0.25 µM (prepared as 2.5 mM in 0.1 N HCl, Cambridge Isotope Laboratories, MSK-A2-1.2), citric acid d4 at 0.02 µg/mL (dissolved at 100 µg/mL in H2O ...
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bioRxiv - Biophysics 2022Quote: ... and 70 mg/L sodium α-ketobutyrate (2H-3, 13C-methyl; Cambridge Isotope Laboratories, Tewksbury, MA). EmrE was purified also as previously reported (8 ...
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bioRxiv - Cancer Biology 2020Quote: ... 10μL of media was mixed with 10μL of a mixture of isotopically labeled metabolites and amino acids of known concentrations (Cambridge Isotope Laboratories, Tewksbury, MA) and extracted with 600μL ice-cold HPLC grade methanol ...
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bioRxiv - Neuroscience 2020Quote: F2 SILAM wide type female mice were purchased from Cambridge Isotope Laboratories and then crossed with regular C57BL6 wild type males by fed with stable isotope labeling using amino acids in cell culture (SILAC) L-lysine (13C6) feed (Cambridge Isotope Laboratories, Inc). After 7 days ...
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bioRxiv - Cancer Biology 2022Quote: ... the cell pellet was resuspended into 1 mL of ice-cold 2:2:1 MeOH: ACN: H2O (v/v/v) containing 10 μM stable isotope-labeled canonical amino acid mix (Cambridge Isotope Laboratories, Inc) as internal standards ...
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bioRxiv - Cancer Biology 2023Quote: ... The tissue powder was then weighed into pre-chilled tubes and extracted with methanol (containing 500 nM each of 17 isotopically labeled 13C/15N amino acids (Cambridge Isotope Laboratories, Inc.)) ...
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bioRxiv - Cell Biology 2020Quote: ... 100 μl volume uptake reactions with 50 μM 13C5-NAD+ (Cambridge Isotope Laboratories) were initiated by adding substrate to 80 μg of mitochondria in incubation media containing 0.1% BSA and 100 μM unlabeled NAD+ at 30°C ...
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bioRxiv - Cancer Biology 2020Quote: ... [3-13C] serine (CLM-1572) and [U-2H]betaine (DLM-407-PK) were purchased from Cambridge Isotope Laboratories and stored in stocks of 10 mg/mL at −20°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Dried samples were rehydrated with 2:1 methanol:water into which a mixture of isotopically labeled amino acids of known concentrations (Cambridge Isotope Laboratories, MSK-A2-1.2) was added ...
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bioRxiv - Physiology 2021Quote: ... continuous infusion of stable amino acid isotope [13C6]-phenylalanine (L-phenylalanine, ring-13C6, 99%, CLM-1055-MPT; Cambridge Isotope Laboratories, Inc., Tewksbury, MA, USA) was used for measurement of amino acid kinetics across the limb and incorporation of labeled phenylalanine into muscle as previously described [20] ...
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bioRxiv - Biochemistry 2021Quote: ... Tissue was incubated in Krebs-Ringer buffer (formulations used in each figure specified below below) supplemented with 5 mM glucose and [U-13C]-succinic acid (Cambridge isotope CLM-1571-0.1) or [U-13C]-malate (Cambridge isotope CLM-8065 ...
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bioRxiv - Cancer Biology 2023Quote: 5 μL of adult bovine serum or ftABSB was mixed with 45 μL of HPLC-grade methanol:water (80:20) including 500 nM 13C/15N labeled amino acids (Cambridge Isotope Laboratories, MSK-CAA-1). Samples were vortexed for 15 min at 4°C and insoluble material was sedimented by centrifugation at 16,000 x g for 10 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 μL of media was mixed with 45 μL of HPLC-grade methanol:water (80:20) including 500 nM 13C/15N labeled amino acids (Cambridge Isotope Laboratories, MSK-CAA-1). Samples were then vortexed for 15 min at 4°C ...
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bioRxiv - Biochemistry 2022Quote: Amino acids were quantified from 10 µL plasma precipitated with 180 µl ice-cold methanol containing 1 nmol of stable isotope labelled amino acids (Cambridge Isotope Laboratories, MSK-A2-1.2). Samples were incubated at -20°C for 30 min followed by centrifugation at 4°C (14,000g ...
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bioRxiv - Microbiology 2022Quote: ... were resuspended in 300 μL of LC/MS grade methanol containing a mixture of 17 isotope-labeled amino acids (200 nM, Cambridge Isotope Laboratories, MSK-A2-1.2). To this solution ...
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bioRxiv - Cell Biology 2022Quote: ... Polar metabolites were extracted in ice cold 80% LC/MS grade methanol containing 15N and 13C fully-labeled amino acid standards (Cambridge Isotope Laboratories #MSK-A2-1.2). Tissues were homogenized by Bead Ruptor 24 (Omni International ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed twice with ice-cold 0.9% NaCl and polar metabolites extracted in ice cold 80% LC/MS grade methanol containing 15N and 13C fully-labeled amino acid standards (Cambridge Isotope Laboratories #MSK-A2-1.2). Extracts were vigorously shaken by vortex for 10 minutes at 4°C and spun at 14,000 rpm at 4°C for 10 minutes to remove insoluble cell debris ...
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bioRxiv - Genomics 2023Quote: ... followed by metabolite extraction in ice cold 80:20 LC/MS grade methanol: water buffer containing 15N and 13C fully-labeled amino acid standards (Cambridge Isotope Laboratories #MSK-A2-1.2). Samples were vortexed for 10 minutes at 4°C and then centrifuged at maximum speed (> 14,000g ...
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bioRxiv - Microbiology 2023Quote: ... 2.8 ml of gas was removed and immediately replaced with 2.8 ml of 15N2 (98% enriched, Cambridge Isotope Laboratories Inc, USA). Vials were overpressured by adding 0.3 ml of air or N2 gas in oxic or anoxic incubations ...
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bioRxiv - Neuroscience 2020Quote: ... with a pH of 7.4 containing 6.15 mM NaN3 and 4.64 mM sodium 3-[trimethylsilyl] d4-propionate (Cambridge Isotope Laboratories) was prepared ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Samples were thawed on ice and phthalates were extracted from a 10 μL aliquot via vigorous vortexing for 30 min at 4°C in the presence of 5:3:2 methanol:acetonitrile:water (240 μL) containing an isotope labeled standard (1 μM DEHP ring-1,2-13C2, Cambridge Isotope Laboratories). Extraction supernatants were clarified via centrifugation at 18,000 rpm for 10 min at 4°C and analyzed immediately by ultra high-pressure liquid chromatography coupled to mass spectrometry (UHPLC-MS) ...
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bioRxiv - Biophysics 2021Quote: ... 100 µL of 140 mM [6,6’-2H2]glucose solution (Cambridge Isotope Laboratories, Tewksbury, MA, USA) was injected into each tube for a final 2H-labeled glucose concentration of 20 mM (and final volume=700 µL ...
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bioRxiv - Biophysics 2021Quote: ... the RNA samples were lyophilized and dissolved in 100% D2O (99.8%, Cambridge Isotope Laboratories, Inc.) and transferred to an Eppendorf tube containing lyophilized buffer (50 mM K-phosphate buffer (pH=7.5 or pH=5.8 ...
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bioRxiv - Biochemistry 2020Quote: ... Spectra were recorded either under conditions of neutral pH in 100% D2O (Cambridge Isotope Laboratories), or following alkalinization (i.e ...
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bioRxiv - Cell Biology 2023Quote: For whole cell proteomics lysine auxotrophic strains were grown in yeast nitrogen base (YNB) medium containing either 30 mg/ml L-Lysine or 30 mg/ ml 13C6;15N2-L-Lysine (Cambridge Isotope Laboratories, USA). Cells were grown to logarithmic phase and 1 OD unit of each strain was harvested ...
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bioRxiv - Cell Biology 2023Quote: Lysine auxotrophic strains were grown in yeast nitrogen base (YNB) medium containing either 30 mg/ml L-Lysine or 30 mg/ml 13C6;15N2-L-Lysine (Cambridge Isotope Laboratories, USA). Cells were grown to logarithmic phase and up to 350 OD600 units were harvested of labeled cells ...
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bioRxiv - Cell Biology 2023Quote: ... lysine auxotrophic strains were grown in yeast nitrogen base (YNB) medium containing either 30 mg/ml L-Lysine or 30 mg/ml 13C6;15N2-L-Lysine (Cambridge Isotope Laboratories, USA). Cells were grown to logarithmic phase and the same amount of OD units of the control strain and the mutant strain were harvested and combined ...
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bioRxiv - Bioengineering 2022Quote: ... 100 μl of 5 M isotope labelled D-Glucose (U-13C6, 99% from Cambridge Isotope Laboratories) prior to feeding ...
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bioRxiv - Biochemistry 2019Quote: ... 20 min) and resuspended in M9 minimal media comprising 3 g/L 15N ammonium chloride (Cambridge Isotope Laboratories, NLM-467-25) with 10 g/L glucose or 10 g/L 13C glucose (Cambridge Isotope Laboratories ...
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bioRxiv - Microbiology 2023Quote: ... Fractions 1–2 were prepared in 100% deuterated H2O (D2O; Cambridge Isotope Laboratories; referenced at 4.39 ppm) and Fractions 3–4 in 10% CD3OD ...
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bioRxiv - Molecular Biology 2021Quote: ... expression was carried out in M9 minimal media supplemented with 3 g/L (13C6, 99%)-D-glucose and/or 1 g/l (15N, 99%)-NH4Cl (Cambridge Isotope Laboratories). To generate isotopically labeled rad6-A126T ...
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bioRxiv - Biophysics 2020Quote: ... harvested at an OD600nm of 3 and resuspended in 2 L of M9 minimal medium with isotopic enrichment (1 g/L of 15NH4Cl and/or 3 g/L of 13C6-glucose, Cambridge Isotope Laboratories). After 30-45 min ...
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bioRxiv - Biochemistry 2023Quote: ... 15N-labeled protein samples were grown in 1L minimal media containing 3 g/L 15N ammonium chloride (Cambridge Isotope Laboratories, Tweksbury, MA) and unlabeled protein samples were grown in 1L super broth media ...
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bioRxiv - Microbiology 2021Quote: ... and 84 mg/mL [13C6,15N4]L-Arginine-HCl (Cambridge Isotope Laboratories) to DMEM Medium for SILAC (Thermo) ...
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bioRxiv - Biophysics 2024Quote: ... The cells were used to inoculate 2L of H2O-based M9 media supplemented with 3 g L-1 of [U-13C6] d-glucose (Cambridge Isotope Laboratories, CIL) and 2 g L-1 of [15N] ammonium chloride (Cambridge Isotope Laboratories ...
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bioRxiv - Biophysics 2021Quote: ... 13C,15N-labelled 24x’ was expressed using a 1/100 dilution of Bioexpress media (Cambridge Isotope Labs, Tewksbury, MA) in labelled M9 media (51) ...
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bioRxiv - Biophysics 2020Quote: ... The buffer for 13C-labeled experiments contained 98% 13-2H MES in 100% 2H2O (Cambridge Isotope Laboratories, Tewksbury, MA).
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bioRxiv - Biochemistry 2023Quote: ... 10 mL Bioexpress Cell Growth Media (Cambridge Isotope Laboratories, Tewksbury, MA, USA), 5 mL Gibco™ MEM Vitamin Solution (100x) ...
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bioRxiv - Biochemistry 2022Quote: ... MMA quantification was performed using 3 uL of plasma that was spiked in with [U-13C]-MMA (Cambridge Isotope Laboratories, cat# CLM-9426-PK) as previously described (31 ...
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bioRxiv - Biochemistry 2020Quote: ... 200 µg Xyl6 was mixed with 4 mM Ac-CoA and 9 µM purified enzyme in 100 mM potassium phosphate buffer (pH 6.8) in D2O (99.9%; Cambridge Isotope Laboratories), and the reaction (110 uL total volume ...
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bioRxiv - Biochemistry 2021Quote: ... 5 mL 15N Bioexpress Cell Growth Medium (Cambridge Isotope Laboratories, Tewksbury, MA, USA), 5 mL of Gibco MEM Vitamin Solution (100x) ...