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Citations for Cambridge Isotope Labs :
351 - 383 of 383 citations for L β Homo β 2 hydroxyphenyl glycine; R 3 Amino 3 2 hydroxyphenyl propanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... irregularis was cultured in modified SC solid medium supplied with 1 mM neutralized [1-13C]myristic acid (Cambridge Isotope Laboratories, Inc.) for 2.5 months ...
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bioRxiv - Biochemistry 2023Quote: RPE cells were incubated in pH 7.4 α-MEM and 50 µM [U-13C16]-palmitic acid (Cambridge isotope labs, CLM-409) complexed to bovine serum albumin at a 6:1 ratio of fatty acid to albumin ...
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bioRxiv - Neuroscience 2024Quote: ... with 0.15% formic acid) containing 10 μM of [13C-U] [15N-U] labelled internal standards (MSK-CAA-1; Cambridge Isotope Laboratories). Analytes were separated using a SeQuant ZIC cHILIC 3μm 100Å 150 x 2.1 mm column (EMD Millipore ...
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Sex-specific fear acquisition following early life stress is linked to amygdala glutamate metabolismbioRxiv - Animal Behavior and Cognition 2024Quote: ... U-13C15N-labeled amino acids at a final concentration of 0.25 µM (prepared as 2.5 mM in 0.1 N HCl, Cambridge Isotope Laboratories, MSK-A2-1.2), citric acid d4 at 0.02 µg/mL (dissolved at 100 µg/mL in H2O ...
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bioRxiv - Neuroscience 2023Quote: ... each rat was fed 1g of a pancake that contained 4 µl of [13C]-octanoic acid (Cambridge Isotope Laboratories, Inc, Tewksbury MA). After visually confirming the rats had eaten the entire pancake (in less than 5min or they were omitted from the study) ...
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bioRxiv - Biophysics 2024Quote: ... 1 L cultures of autoinduction media (Cold Spring Harbor Protocols) were used with 15N labeled ammonium chloride (Cambridge Isotope Laboratories). The final antibiotic concentration was increased to 0.1 mg/mL of kanamycin to reduce plasmid loss and poor yields ...
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bioRxiv - Neuroscience 2024Quote: ... Heavy SILAC media was supplemented with L-Lysine:2HCl (13C6, 99%; 15N2, 99%) (Cambridge Isotope Laboratories, Inc., CNLM-291-H) and L-Arginine:HCl (13C6 ...
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bioRxiv - Neuroscience 2024Quote: ... On day 8, mice were switched to Mouse Express L-Lysine (13C6, 99%) irradiated mouse feed (Cambridge Isotope Laboratories, USA) for 8 days at libitum ...
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bioRxiv - Immunology 2020Quote: ... 25 μl of serum and 0.5 ml of Lipid Extraction Buffer containing 1 μg of D2-oleic acid (Cambridge isotope: DLM-689-0.1) and 1 μg of D14-palmitoleic acid (Cayman ...
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bioRxiv - Microbiology 2023Quote: ... were prepared via protein precipitation with the addition of 9 volumes of 74.9:24.9:0.2 v/v/v acetonitrile/methanol/formic acid containing stable isotope-labeled internal standards (valine-d8, Sigma-Aldrich; and phenylalanine-d8, Cambridge Isotope Laboratories). The samples were centrifuged (10 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 µL of biofluid samples or serial dilutions of a chemical standard library of 149 metabolites were extracted with 45 µL of a 75:25:0.1 HPLC grade acetonitrile:methanol:formic acid extraction mix that included the following labeled stable isotope internal standards:13C-labeled yeast extract (Cambridge Isotope Laboratory, Andover, MA, ISO1), 2H9 choline (Cambridge Isotope Laboratory ...
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bioRxiv - Biophysics 2021Quote: ... The cells were collected by centrifugation and resuspended in M9 medium (1 L, 100% D2O) supplemented with 15NH4Cl (Cambridge Isotope Laboratories) and 12C6H12O6 as the sole nitrogen and carbon sources ...
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bioRxiv - Molecular Biology 2020Quote: ... cultures were first grown at 37°C in 1 L of LB media until an OD600 of 0.6 before the cells were pelleted and resuspended in 0.5 L M9 minimal media prepared in D2O supplemented with 13C-D-glucose and 15NH4Cl (Cambridge Isotope Laboratories). After 1 hour recovery at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... A pulse of 0.025% (w/v) stable isotope-labeled lysine derivative 13C615N2 L-lysine (“heavy” lysine, Lys8, K8, Cambridge Isotope Laboratories) was then added in the presence of ampicillin and 1 – 2 mL of cultures were harvested in intermittent time intervals (10min - 24h ...
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bioRxiv - Biochemistry 2022Quote: ... #CLM-893), and L-tryptophan (13C11, 99%; #CLM-4290) and tracer glucose (13C6, 99%, #CLM-1396) were obtained from Cambridge Isotope Laboratories ...
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bioRxiv - Microbiology 2023Quote: ... was pipetted into 1.7 mL tubes and was then layered with 150 µL of 13C5 15N2- L-Gln (Cambridge Isotope Laboratories) to yield a final concentration of 50 µM when mixed with cells ...
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bioRxiv - Pathology 2024Quote: ... dissolved in sterile PBS or 0.5 mg/gram of body weight 13C3 Sodium L-lactate (CLM-1579-0.5, 20% w/w in water, Cambridge Isotope Laboratories). Twenty minutes after injection mice were euthanized via exsanguination by cardiac puncture under non-recovery isoflurane anesthesia ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... were diluted using 90 µL of 74.9:24.9:0.2 v/v/v acetonitrile/methanol/formic acid containing stable isotope-labeled internal standards (valine-d8, Isotec; and phenylalanine-d8, Cambridge Isotope Laboratories; Andover, MA). The samples were centrifuged (10 min ...
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bioRxiv - Biochemistry 2021Quote: ... Tissue was incubated in Krebs-Ringer buffer (formulations used in each figure specified below below) supplemented with 5 mM glucose and [U-13C]-succinic acid (Cambridge isotope CLM-1571-0.1) or [U-13C]-malate (Cambridge isotope CLM-8065 ...
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bioRxiv - Biochemistry 2021Quote: ... 15N-labeled SARS-CoV-2 nsp7 sample was expressed and purified by the same methods as previously described in our NMR assignment paper.20 The recombinant Escherichia coli Rossetta2 pLysS was first grown in a M9 minimal medium containing 1 g/L 15N4HCl (Cambridge Isotope Laboratories), 4 g/L 13C6-D-Glucose (Cambridge Isotope Laboratories ...
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bioRxiv - Physiology 2024Quote: ... the cells were placed in 10 cm plates with media where the normal glucose was replaced with 13C6-L-glucose (Cambridge Isotope Laboratories). Cells were allowed to incubate in the 13C6-L-glucose prior to the in vitro ischemic-reperfusion injury (hypoxia/reoxygenation ...
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bioRxiv - Microbiology 2024Quote: ... 1 g/L of either (NH4)2SO4 (designated as 14N throughout the text) or (15NH4)2SO4 (designated as 15N, Cambridge Isotope Laboratories) was used as a source of nitrogen ...
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Multi-omics characterization of partial chemical reprogramming reveals evidence of cell rejuvenationbioRxiv - Molecular Biology 2023Quote: ... Protein was precipitated from 10 µl of sample by addition of nine volumes of 74.9:24.9:0.2 v/v/v acetonitrile/methanol/formic acid containing internal standards (valine-d8, Sigma-Aldrich; St. Louis, MO; and phenylalanine-d8, Cambridge Isotope Laboratories; Andover, MA). Precipitated material was cleared by centrifugation and the supernatant was injected directly onto a 150 x 2 mm 3 µm Atlantis HILIC column (Waters ...
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bioRxiv - Molecular Biology 2021Quote: ... For the 15N isotope labeling cells were cultivated at 37 °C in M9 minimal medium with 1 g/L 15NH4Cl (Cambridge Isotope Laboratories, Inc.).
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bioRxiv - Physiology 2023Quote: ... hearts were perfused for 20 minutes with a modified KH-buffer containing uniformly labelled glucose (5 mmol/L [U-13C]glucose, Cambridge Isotope Laboratories, Inc.) instead of standard glucose ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were cultured in glucose and glutamine-free RPMI-1640 media supplemented with 1 mM labeled 13C5-L-Glutamine (Cambridge Isotope Laboratories Inc.), 10 mM unlabeled Glucose for 16 hours in triplicate ...
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bioRxiv - Biochemistry 2022Quote: ... a 100 mL LB starter culture treated with 100 μg/mL ampicillin was grown at 30 °C overnight and was used to inoculate 4× 1 L of M9 minimal medium containing 15NH4Cl and/or 13C6-glucose (Cambridge Isotope, Tewksbury, MA, USA). The cells were grown in this isotopically-enriched minimal media at 37 °C and shaken at 200 rpm until the OD600 reached 0.6-0.8 before a brief cold shock at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... with and without supplementation with 1 mM of stable-isotope enriched 13C L-DOPA (RING-13C6, 99%, CLM-1007-0, Cambridge Isotope Laboratories, MA, USA) for 7 d ...
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Metabolic function in aging retina and retinal pigment epithelium remains robust despite vision lossbioRxiv - Biochemistry 2021Quote: Either 5 mM U-13C glucose alone or 5 mM unlabeled glucose and 2 mM U-13C glutamine U-13C metabolic tracers (99% isotopic purity) included 13C labeled D-Glucose and L-Glutamine (Cambridge Isotope Laboratories, Inc; Tewksbury, MA). Incubations ...
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bioRxiv - Cell Biology 2020Quote: ... 42 mg/L arginine hydrochloride (13C6, 15N4-arginine in ‘heavy’ media), and 146 mg/L lysine hydrochloride (13C6, 15N2-lysine in ‘heavy’ media) (Cambridge Isotope Laboratories, Tewksbury, MA, USA). Stable tetracycline-inducible cell line (FLAGOCIAD2 ...
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bioRxiv - Physiology 2022Quote: ... Three fish per tank per condition were injected with a 150 mM phenylalanine (PHE) solution containing 50% ring-[D5]-L-phenylalanine ([D5]-PHE, 98%, Cambridge Isotope Laboratories, Inc. Andover, USA) at a dose of 1 mL per 100 g of body mass and returned to their tanks for an incorporation period of three hours ...
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bioRxiv - Cancer Biology 2023Quote: ... Tracing of glutamine was performed by supplementing no-glucose and no-glutamine media with 4 mM 13C5 L-glutamine (Cambridge Isotope Laboratories, Inc., CLM-1822-H) and 5 mM unlabeled glucose ...
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bioRxiv - Developmental Biology 2022Quote: ... and L-Lysine (0.2 mM) were replaced with the non-radioactive isotopic forms Arg-10 (13C6 H14 15N4O2; Cambridge Isotope Laboratories, cat. no. CNLM-539-H-PK) and Lys-8 (13C6 H14 15N2O2 ...