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Citations for Cambridge Isotope Labs :
301 - 345 of 345 citations for D Galactose U 13C6 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... and DMEM without glucose and pyruvate supplemented with 10% dialyzed FBS and 10 mM U-13C-glucose (Cambridge Isotope Laboratories) was added for 24 hours prior to metabolite extraction.
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bioRxiv - Immunology 2023Quote: ... On the final day all media conditions were replaced with 5 mM [U-13C]-fructose (Cambridge Isotope Laboratories, CLM-1553). Metabolites were extracted using 80% methanol 24 hours later ...
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bioRxiv - Cancer Biology 2023Quote: ... 50 μL of internal standard solution (U-13C-15N-cysteine, catalog no: CNLM-3871-H-PK, Cambridge Isotope Laboratories catalog) was added ...
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bioRxiv - Cancer Biology 2024Quote: ... and then switched into normal DMEM containing 4.5 mg/mL D-Glucose (DLM-2062, Cambridge Isotope Laboratories) + 1mM Sodium Pyruvate (Catalog # ...
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bioRxiv - Microbiology 2020Quote: ... with 9.5 mM NH4Cl or 15NH4Cl and 0.2% (w/v) carbon source (acetate, succinate, malate, glycerol, glucose, U-13C-glucose (Cambridge Isotope Laboratories) or glucose supplemented with 0.1% Cas-amino acids) ...
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bioRxiv - Cancer Biology 2022Quote: ... Omental tumor biopsies from HGSOC patients were sectioned (0.5-1 mm) and incubated in DMEM supplemented with 10 mM [U-13C]-glucose (Cambridge Isotope Laboratories) and 2 mM glutamine ...
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bioRxiv - Biochemistry 2023Quote: RPE cells were incubated in pH 7.4 α-MEM and 50 µM [U-13C16]-palmitic acid (Cambridge isotope labs, CLM-409) complexed to bovine serum albumin at a 6:1 ratio of fatty acid to albumin ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were seeded and treated as above and incubated in media in which glutamine was replaced with [U-13C]-glutamine (99% enrichment; Cambridge Isotope Laboratories ...
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bioRxiv - Physiology 2024Quote: Mice were fasted for 2 hours then injected intraperitoneally (i.p.) with 1g/kg uniformly labelled U-13C-glucose (CLM-1396, Cambridge Isotope Labs) in saline ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were seeded and treated as above and incubated in media in which glutamine or glucose was replaced with [U-13C]-glutamine (99% enrichment; Cambridge Isotope Laboratories ...
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bioRxiv - Cancer Biology 2024Quote: ... the cell lines were cultured in glucose- and glutamine-free RPMI-1640 media supplemented with 10 mM uniformly labeled U-13C-glucose (Cambridge Isotope Laboratories Inc. ...
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bioRxiv - Cancer Biology 2024Quote: ... tumor-bearing mice were fasted for 16 hours before being infused with [U-13C]-β-OHB (Cambridge Isotope Laboratories, CLM-3853) through a tail vein catheter ...
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bioRxiv - Microbiology 2023Quote: ... Biomass was scraped into 20ml flask of Celtone-R4 broth (10 g D-glucose; Cambridge Isotope Laboratories (CLM) #CLM-1396-5 ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were washed with PBS and fed 1 ml of culture medium supplemented with 2 mM [U-13C]glutamine (CLM-1822, Cambridge Isotope Laboratories) or 12.5 mM [1,2-13C]glucose (CLM-504-1 ...
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bioRxiv - Immunology 2021Quote: ... complete CTL:AIM-V labeling medium consisted of glucose-free CTL:AIM-V with 5% heat-inactivated human AB serum supplemented with 11 mM uniformly labeled 13C-glucose ([U-13C]glucose) (Cambridge Isotope Laboratories). Glucose-free ImmunoCult™-XF T Cell Expansion Medium (STEMCELL Technologies ...
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bioRxiv - Immunology 2024Quote: ... the media was changed to DMEM media containing 1% dialyzed FBS or 1% dialyzed FBS supplemented with 2mM [U-13C8]-octanoate (Cambridge Isotope Laboratories). After 24h ...
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bioRxiv - Cancer Biology 2024Quote: ... the media was removed and replaced with glutamine-free media supplemented with 10% dFBS and 4 mmol/L U-13C5-L-glutamine (CLM-1822-H, Cambridge Isotope Laboratories) for 2 hours prior to harvest (n = 3 per condition) ...
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bioRxiv - Cancer Biology 2022Quote: ... 3×106 cells/well were seeded in a 6-well plate and cultured in 2 mL assay media supplemented with 10% dialyzed FBS (Wisent) and either 25 mM U-[13C]-glucose (Cambridge Isotope Laboratories) and 4 mM glutamine (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: The cell lines were cultured in glucose-free RPMI-1640 media supplemented with 5% dialyzed FBS and P/S and 10 mM of 13C-U-Glucose (CLM-1396, Cambridge Isotope Laboratories). The cells were collected at 8 ...
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bioRxiv - Cancer Biology 2022Quote: ... Dried pellet from unlabeled samples was mixed with [U13C]-D/L-2HG (internal standard for unlabeled samples, Cambridge isotope laboratories ...
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bioRxiv - Biochemistry 2024Quote: ... The culture was diluted one-to-one with M9/D2O medium (prepared with D-[2H;12C]-glucose, Cambridge Isotope Laboratories ...
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bioRxiv - Plant Biology 2022Quote: ... six leaf discs from the same leaf were transferred in a well of a 6-well microplate filled with 4 mL/well of a buffer containing 10 mM MES-KOH (pH 6.5) and 10 mM U-13C-pyruvate (13C isotopic purity of 99%; sodium salt from Cambridge Isotope Laboratories (Eurisotop, France)) ...
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bioRxiv - Physiology 2023Quote: ... hearts were perfused for 20 minutes with a modified KH-buffer containing uniformly labelled glucose (5 mmol/L [U-13C]glucose, Cambridge Isotope Laboratories, Inc.) instead of standard glucose ...
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bioRxiv - Cancer Biology 2024Quote: ... Alternatively post 48 hr LNCaP monoclonal CRISPR KOs were washed with PBS and media was replaced with DMEM (Dulbecco’s Modified Eagle’s Medium) medium containing 10 mM [U-13C]-glucose (Cambridge Isotope laboratories: #CLM-1396-10), 2 mM unlabeled glutamine and 10% dialyzed FBS (in triplicate) ...
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bioRxiv - Cancer Biology 2024Quote: ... organoid cultures were washed with PBS and media was replaced with DMEM (Dulbecco’s Modified Eagle’s Medium) medium containing 15 mM [U-13C]-glucose (Cambridge Isotope laboratories: #CLM-1396-10), 2 mM unlabeled glutamine ...
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bioRxiv - Molecular Biology 2022Quote: ... supplemented with 15NH4Cl and when needed 13C-D-glucose as sole nitrogen and carbon sources (purchased from Cambridge Isotope Laboratories). The cells were grown at 37°C before they were induced with 0.2 mM IPTG at an OD600 of 1.2 for TB and 0.8 for M9 minimal medium and incubated at 17°C for 16 h.
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bioRxiv - Biochemistry 2021Quote: ... Tissue was incubated in Krebs-Ringer buffer (formulations used in each figure specified below below) supplemented with 5 mM glucose and [U-13C]-succinic acid (Cambridge isotope CLM-1571-0.1) or [U-13C]-malate (Cambridge isotope CLM-8065 ...
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bioRxiv - Biochemistry 2022Quote: ... MMA quantification was performed using 3 uL of plasma that was spiked in with [U-13C]-MMA (Cambridge Isotope Laboratories, cat# CLM-9426-PK) as previously described (31 ...
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Structural Model of Bacteriophage P22 Scaffolding Protein in a Procapsid by Magic-Angle Spinning NMRbioRxiv - Biochemistry 2024Quote: ... cells were grown in M9 minimal media supplemented with 1 g/L 15NH4Cl or 3 g/L U-13C glucose (Cambridge Isotope Laboratories, Cambridge, MA). After inoculation of M9 media using a starter culture ...
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bioRxiv - Microbiology 2022Quote: ... 99% [2,3-13C] sodium succinate or an equimolar molar 1:1 mixture of [U-13C] sodium succinate (obtained from Cambridge Isotope Laboratories, Inc., Andover, MA, USA) and naturally labelled sodium succinate.
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bioRxiv - Biochemistry 2022Quote: ... Cell pellets were resuspended in 250 mL of isotopically labelled minimal media M9 salt solution supplemented with 13C D-glucose 4.0 g/L and 15NH4Cl 1.0 g/L (Cambridge Isotope Laboratories, Inc.), then incubated to allow the recovery of growth and clearance of unlabeled metabolites ...
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bioRxiv - Molecular Biology 2020Quote: ... cultures were first grown at 37°C in 1 L of LB media until an OD600 of 0.6 before the cells were pelleted and resuspended in 0.5 L M9 minimal media prepared in D2O supplemented with 13C-D-glucose and 15NH4Cl (Cambridge Isotope Laboratories). After 1 hour recovery at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... 450 μL of supernatant were transferred to a new tube and 150 μL of deuterated water were added (D2O, D, 99.9%, Cambridge Isotope Laboratories). Each sample was vortex-mixed for 1 min before transferring into 5 mm SampleJet NMR tubes ...
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bioRxiv - Biophysics 2022Quote: HDX samples of I552A SLO-1 were exchanged as a function of time by a 10-fold dilution into D2O (99% D; Cambridge Isotope) buffer ...
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bioRxiv - Biophysics 2024Quote: ... 4.0 gL-1 of D-glucose provided in specific isotopic ratios (12C, Merek, Germany; 13C, Cambridge Isotope Laboratories, USA; Table S1). 109 cells were harvested at late-exponential growth phase (Figure S3) ...
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bioRxiv - Biophysics 2021Quote: ... Deuterated RNA samples were prepared in the same way using selective deuterated rNTPs (D-H5, H3′, H4′, H5′ and H5′′) and 13C/15N-labeled samples were prepared using labeled rNTPs (Cambridge Isotope Laboratories). The resulting RNAs were purified for NMR studies by gel electrophoresis ...
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bioRxiv - Microbiology 2021Quote: ... and Rpk9-I53-50A trimers were H/D exchanged (HDX) in the deuteration buffer (pH* 7.5, 85% D2O, Cambridge Isotope Laboratories, Inc.) for 3 ...
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bioRxiv - Immunology 2020Quote: 3 µg of monomeric RBD and RBD-8GS-I53-50A were incubated and H/D exchanged (HDX) in the deuteration buffer (pH* 7.6, 85% D2O, Cambridge Isotope Laboratories, Inc.) for 3 ...
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bioRxiv - Biophysics 2024Quote: ... M9 minimal media containing 1 g/L of 15NH4Cl and 3 g/L of [13C-6]-D-glucose (or natural abundance glucose) (Cambridge Isotope Laboratories) were used ...
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bioRxiv - Microbiology 2021Quote: ... uniformly 15 N- and 13 C-labeled proteins were produced by growing cell cultures in M9 minimal medium that contained 1 g/l 15 N-ammonium chloride and 2 g/l 13 C-D-glucose (Cambridge Isotope Laboratories, Inc.) as the sources of nitrogen and carbon ...
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bioRxiv - Biochemistry 2021Quote: ... 13C-1 labelled polyM was produced from fermentation of the same strain using 13C-1 (99 % 13C) D-fructose (Cambridge Isotope Laboratories, USA). This substrate was further subjected to acid hydrolysis as described elsewhere (39 ...
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bioRxiv - Molecular Biology 2020Quote: ... Unlabeled samples were prepared using Luria Broth (LB) (Melford) and minimal media M9 with 15NH4Cl and/or D- [13C] glucose (Cambridge Isotope Laboratories, Inc) were used to prepare the labeled samples (25) ...
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bioRxiv - Biophysics 2024Quote: ... The 1 L M9 culture contained 1 g of 15N labelled ammonium chloride and 2 g of 13C uniformly labelled α-D glucose (Cambridge Isotope Laboratories). The M9 culture was incubated for 30 min with shaking at 37 °C and 220 RPM before adding IPTG to 0.5 mM ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were prepared using 500uL of sample mixed with 500uL of an NMR buffer containing a chemical shift reference and analytical standard (TSP-2, 2, 3, 3-d4 (D,98% Sodium-3-Trimethylsilyl-Propionate, Cambridge Isotope Laboratories, Inc.). The NMR buffer was prepared with Mono- and Dibasic Potassium Phosphate at 0.5M concentration and a pH of 7 ...
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bioRxiv - Biophysics 2023Quote: ... The exo-PS accumulated on the 10 kDa membrane surface as a clear gel and was collected and dialyzed extensively against MilliQ-grade H2O or D2O (Cambridge Isotope Labs, 99.9% D). The H2O solutions provide scattering profiles at an additional contrast ...