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Citations for Cambridge Isotope Labs :
251 - 300 of 313 citations for cis Clopidogrel MP 13C d3 Derivative Pair of Enantiomers since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... 4.0 gL-1 of D-glucose provided in specific isotopic ratios (12C, Merek, Germany; 13C, Cambridge Isotope Laboratories, USA; Table S1). 109 cells were harvested at late-exponential growth phase (Figure S3) ...
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bioRxiv - Biophysics 2024Quote: ... to obtain the 15N uniformly single labelled protein or in a media containing 15NH4Cl (0.1% w/v) and 13C-labelled glucose (0.4% w/v) (Cambridge isotope Laboratories, Inc.) for 15N and 13C uniformly double labelled protein ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were seeded and treated as above and incubated in media in which glutamine was replaced with [U-13C]-glutamine (99% enrichment; Cambridge Isotope Laboratories ...
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bioRxiv - Biophysics 2023Quote: ... except that yeast cells were grown in SD-CSM media made with 2% (w/v) protonated 13C-depleted glucose (99.9% 12C, Cambridge Isotope Labs) as the carbon source ...
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bioRxiv - Cancer Biology 2023Quote: U-[13C]-Glc labelling media consisted of DMEM supplemented with 10 mM D-glucose (U-13C6; CLM-1396; Cambridge Isotope Laboratories), 2 mM glutamine ...
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bioRxiv - Cancer Biology 2023Quote: ... U-[13C]-Q labelling media consisted of DMEM supplemented with 2 mM L-glutamine (U-13C5; CLM-1822; Cambridge Isotope Laboratories), 10 mM glucose ...
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bioRxiv - Immunology 2022Quote: ... and re-cultured (2.5 × 106 cells/well in 24-well plates) for indicated times in medium containing 13C-labeled metabolites (Cambridge Isotope Laboratories) at the following concentrations ...
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bioRxiv - Cancer Biology 2022Quote: ... Omental tumor biopsies from HGSOC patients were sectioned (0.5-1 mm) and incubated in DMEM supplemented with 10 mM [U-13C]-glucose (Cambridge Isotope Laboratories) and 2 mM glutamine ...
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bioRxiv - Cancer Biology 2023Quote: ... Two transitions were optimised using an authentic standard of glycocholic acid (glycine-1-13C, CLM-191-PK, Cambridge Isotope Laobratories, UK), from the negative precursor ion (m/z 465 ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were seeded and treated as above and incubated in media in which glutamine or glucose was replaced with [U-13C]-glutamine (99% enrichment; Cambridge Isotope Laboratories ...
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bioRxiv - Neuroscience 2024Quote: ... with 0.15% formic acid) containing 10 μM of [13C-U] [15N-U] labelled internal standards (MSK-CAA-1; Cambridge Isotope Laboratories). Analytes were separated using a SeQuant ZIC cHILIC 3μm 100Å 150 x 2.1 mm column (EMD Millipore ...
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bioRxiv - Physiology 2024Quote: Mice were fasted for 2 hours then injected intraperitoneally (i.p.) with 1g/kg uniformly labelled U-13C-glucose (CLM-1396, Cambridge Isotope Labs) in saline ...
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bioRxiv - Cancer Biology 2024Quote: ... TCA cycle standard mix 1 and 2 (cat no. MSK-TCA1 and MSK-TCA 2A) and its 13C-labeled metabolites were purchased from Cambridge Isotope Laboratories ...
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bioRxiv - Cancer Biology 2024Quote: ... the cell lines were cultured in glucose- and glutamine-free RPMI-1640 media supplemented with 10 mM uniformly labeled U-13C-glucose (Cambridge Isotope Laboratories Inc. ...
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bioRxiv - Cancer Biology 2024Quote: ... tumor-bearing mice were fasted for 16 hours before being infused with [U-13C]-β-OHB (Cambridge Isotope Laboratories, CLM-3853) through a tail vein catheter ...
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bioRxiv - Biochemistry 2022Quote: ... 1 % P/S and isotope-labelled D3-15N-L-serine (1 mM unless indicated otherwise) and D4-L-alanine 2 mM (Cambridge Isotope Laboratories, MA, USA). Cells were grown further in the labelling media for 16 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were washed with PBS and fed 1 ml of culture medium supplemented with 2 mM [U-13C]glutamine (CLM-1822, Cambridge Isotope Laboratories) or 12.5 mM [1,2-13C]glucose (CLM-504-1 ...
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bioRxiv - Cell Biology 2022Quote: ... Polar metabolites were extracted in 80 % methanol containing 15N and 13C fully-labeled amino acid standards (MSK-A2-1.2, Cambridge Isotope Laboratories, Inc). Extracts were shaken for 10 min with a vortexer ...
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bioRxiv - Developmental Biology 2020Quote: Larvae collected at 0 hpe were separated into four 1.5 mL tubes each containing 10 individuals and incubated with 1.2 mL FSW + 1.2 μl 10 mM 13C-arginine (Lot No. PR-21659, Catalog No. CLM-2070-0, Cambridge Isotope Laboratories, Inc.). From each tube ...
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bioRxiv - Biochemistry 2021Quote: ... The labeled M9 minimal medium had the same composition except for the replacement of NH4Cl and glucose by [15N]NH4Cl and uniformly labeled [13C]glucose at the same concentrations (99% labeling for both compounds; Cambridge Isotope Laboratories). The growth media were sterilized by filtration (Millex-LG 0.2 µm ...
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bioRxiv - Biophysics 2021Quote: ... Deuterated RNA samples were prepared in the same way using selective deuterated rNTPs (D-H5, H3′, H4′, H5′ and H5′′) and 13C/15N-labeled samples were prepared using labeled rNTPs (Cambridge Isotope Laboratories). The resulting RNAs were purified for NMR studies by gel electrophoresis ...
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bioRxiv - Cell Biology 2021Quote: ... and 500 μl of ice-cold 80% methanol in water with 250nM 13C/15N labeled amino acid standards (MSK-A2-1.2: Cambridge Isotope Laboratories, Inc.) was added to each well ...
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bioRxiv - Microbiology 2021Quote: ... after which the filters were transferred to solid modified Sauton’s medium with butyric acid or 13C-labelled butyric acid (Cambridge Isotope Laboratories, Inc) at a final concentration of 2.5 mM for 24 h ...
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bioRxiv - Biophysics 2020Quote: ... Cells were pelleted and transferred to M9 media containing 1 g of 15N-ammonium chloride and 2 g of 13C uniformly label glucose (Cambridge Isotope Laboratories). After transfer ...
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bioRxiv - Neuroscience 2023Quote: ... each rat was fed 1g of a pancake that contained 4 µl of [13C]-octanoic acid (Cambridge Isotope Laboratories, Inc, Tewksbury MA). After visually confirming the rats had eaten the entire pancake (in less than 5min or they were omitted from the study) ...
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bioRxiv - Cancer Biology 2022Quote: ... 3×106 cells/well were seeded in a 6-well plate and cultured in 2 mL assay media supplemented with 10% dialyzed FBS (Wisent) and either 25 mM U-[13C]-glucose (Cambridge Isotope Laboratories) and 4 mM glutamine (Sigma ...
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bioRxiv - Immunology 2023Quote: ... activated T cells were washed with PBS and re-cultured in TCM (lacking glucose and serine) containing 10% dialyzed FBS and uniformly labeled [13C]-Glucose (Cambridge Isotope Laboratories). T cells (5 x 106 per well in 6 well plates ...
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bioRxiv - Cancer Biology 2023Quote: ... Media was aspirated from cells and then washed rapidly in ice cold bank saline followed by addition of 500 mL ice-cold 80% methanol in water containing 500 nM each of 17 isotopically labeled 13C/15N amino acids (Cambridge Isotope Laboratories). Alternatively ...
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bioRxiv - Cancer Biology 2024Quote: The cell lines were cultured in glucose-free RPMI-1640 media supplemented with 5% dialyzed FBS and P/S and 10 mM of 13C-U-Glucose (CLM-1396, Cambridge Isotope Laboratories). The cells were collected at 8 ...
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bioRxiv - Physiology 2024Quote: Metabolomics was performed on microglia by first washing microglia primary cell cultures with PBS and re-cultured in DMEM (lacking glucose) containing 10% dialyzed FBS and uniformly labeled [13C]-Glucose (Cambridge Isotope Laboratories). Microglia (2 x 106 per well in 6 well plates ...
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bioRxiv - Biochemistry 2024Quote: ... the media was supplemented with: 1) 50 mg/L 2-keto-3-[D2],4-[13C]-butyrate (Cambridge Isotope Laboratories, Inc. CDLM-7318) and 100 mg/L 2-keto-3-[D]-[13CH3,12CD3]- isovalerate (Cambridge Isotope Laboratories ...
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bioRxiv - Biophysics 2024Quote: ... M9 minimal media containing 1 g/L of 15NH4Cl and 3 g/L of [13C-6]-D-glucose (or natural abundance glucose) (Cambridge Isotope Laboratories) were used ...
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bioRxiv - Biophysics 2024Quote: ... the 50 μL cell pellet was mixed with 50 μL of perdeuterated 1x PBS containing AMUPol (Cortecnet, USA) and 18 μL d8- 13C-depleted glycerol (Cambridge Isotope Labs). The 118 μL cell suspension had a final sample matrix of 15:75:10 d8-13C-depleted glycerol:D2O:H2O (v/v/v ...
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bioRxiv - Molecular Biology 2020Quote: ... Unlabeled samples were prepared using Luria Broth (LB) (Melford) and minimal media M9 with 15NH4Cl and/or D- [13C] glucose (Cambridge Isotope Laboratories, Inc) were used to prepare the labeled samples (25) ...
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bioRxiv - Biophysics 2020Quote: ... while 15N and 15N,13C samples for NMR studies were produced using minimal medium (M9) containing 0.1% 15NH4Cl (Cambridge Isotope Laboratories, Tewksbury, MA) or 0.1% 15NH4Cl and 0.3% U-13C-D-glucose (Cambridge Isotope Laboratories ...
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bioRxiv - Cancer Biology 2023Quote: ... The tissue powder was then weighed into pre-chilled tubes and extracted with methanol (containing 500 nM each of 17 isotopically labeled 13C/15N amino acids (Cambridge Isotope Laboratories, Inc.)) ...
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bioRxiv - Biochemistry 2022Quote: ... and sortase A-compatible proteins were either expressed in Terrific Broth (TB) media or M9 media supplemented with 13C-glucose and/or 15NH4Cl (Cambridge Isotope Labs, Inc.) followed by protein induction with 0.5 mM isopropyl ß-D-1-thiogalactopyranoside (Gold Biotechnology ...
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bioRxiv - Physiology 2023Quote: ... hearts were perfused for 20 minutes with a modified KH-buffer containing uniformly labelled glucose (5 mmol/L [U-13C]glucose, Cambridge Isotope Laboratories, Inc.) instead of standard glucose ...
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bioRxiv - Microbiology 2024Quote: ... A single colony was then used to inoculate 100 mL of fresh M9 medium (Helmholtz Center Munich, see Supplementary Information S1) containing 0.4 % 13C-glucose (Cambridge Isotope Laboratories Inc, USA). The inoculated liquid culture was incubated in a 250mL baffled Erlenmeyer flask on shaker at 150 rpm at 37 °C for 24 h ...
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bioRxiv - Cancer Biology 2024Quote: ... Alternatively post 48 hr LNCaP monoclonal CRISPR KOs were washed with PBS and media was replaced with DMEM (Dulbecco’s Modified Eagle’s Medium) medium containing 10 mM [U-13C]-glucose (Cambridge Isotope laboratories: #CLM-1396-10), 2 mM unlabeled glutamine and 10% dialyzed FBS (in triplicate) ...
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bioRxiv - Cancer Biology 2024Quote: ... organoid cultures were washed with PBS and media was replaced with DMEM (Dulbecco’s Modified Eagle’s Medium) medium containing 15 mM [U-13C]-glucose (Cambridge Isotope laboratories: #CLM-1396-10), 2 mM unlabeled glutamine ...
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bioRxiv - Cell Biology 2024Quote: PASMCs were seeded into a 6-well plate and labeled in complete fresh SmBM-2 medium supplemented with 1 mM uniformly [13C]-labeled KIC (13C6-KIC) isotope (cat # CLM-4785-0.1, Cambridge Isotope Laboratories; Tewksbury, MA) for 8 hours ...
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bioRxiv - Biophysics 2024Quote: ... The 1 L M9 culture contained 1 g of 15N labelled ammonium chloride and 2 g of 13C uniformly labelled α-D glucose (Cambridge Isotope Laboratories). The M9 culture was incubated for 30 min with shaking at 37 °C and 220 RPM before adding IPTG to 0.5 mM ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 µL of biofluid samples or serial dilutions of a chemical standard library of 149 metabolites were extracted with 45 µL of a 75:25:0.1 HPLC grade acetonitrile:methanol:formic acid extraction mix that included the following labeled stable isotope internal standards:13C-labeled yeast extract (Cambridge Isotope Laboratory, Andover, MA, ISO1), 2H9 choline (Cambridge Isotope Laboratory ...
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bioRxiv - Biochemistry 2021Quote: ... Tissue was incubated in Krebs-Ringer buffer (formulations used in each figure specified below below) supplemented with 5 mM glucose and [U-13C]-succinic acid (Cambridge isotope CLM-1571-0.1) or [U-13C]-malate (Cambridge isotope CLM-8065 ...
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bioRxiv - Cancer Biology 2023Quote: 5 μL of adult bovine serum or ftABSB was mixed with 45 μL of HPLC-grade methanol:water (80:20) including 500 nM 13C/15N labeled amino acids (Cambridge Isotope Laboratories, MSK-CAA-1). Samples were vortexed for 15 min at 4°C and insoluble material was sedimented by centrifugation at 16,000 x g for 10 min at 4°C ...
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bioRxiv - Immunology 2023Quote: WT and Slc2a5−/− P7 neonatal mice born to dams on HF or CD were injected intraperitoneally via the quadriceps with 4 g/kg [2-13C]-fructose (Cambridge Isotope Laboratories, CLM-1527) in PBS (20 mg in 200 μL PBS) ...
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bioRxiv - Biochemistry 2022Quote: ... MMA quantification was performed using 3 uL of plasma that was spiked in with [U-13C]-MMA (Cambridge Isotope Laboratories, cat# CLM-9426-PK) as previously described (31 ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 μL of media was mixed with 45 μL of HPLC-grade methanol:water (80:20) including 500 nM 13C/15N labeled amino acids (Cambridge Isotope Laboratories, MSK-CAA-1). Samples were then vortexed for 15 min at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... The dimethylation reaction was carried out by adding 2 µL of a 4% formaldehyde solution (Formaldehyde 13C, d2 solution, 596388, Sigma-Aldrich or Formaldehyde-D2, DLM-805-PK, Cambridge Isotope Laboratories Inc.) and 2 µL of a 500 mM NaCNBH3 solution per 100 µL sample at 37°C for 4 h ...