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Citations for Cambridge Isotope Labs :
201 - 228 of 228 citations for N BOC 3 FLUORO L TYROSINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... was pipetted into 1.7 mL tubes and was then layered with 150 µL of 13C5 15N2- L-Gln (Cambridge Isotope Laboratories) to yield a final concentration of 50 µM when mixed with cells ...
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bioRxiv - Cancer Biology 2023Quote: ... U-[13C]-Q labelling media consisted of DMEM supplemented with 2 mM L-glutamine (U-13C5; CLM-1822; Cambridge Isotope Laboratories), 10 mM glucose ...
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bioRxiv - Cancer Biology 2020Quote: ... [3-13C] serine (CLM-1572) and [U-2H]betaine (DLM-407-PK) were purchased from Cambridge Isotope Laboratories and stored in stocks of 10 mg/mL at −20°C ...
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bioRxiv - Cell Biology 2019Quote: Cells were grown in SCD-Lys medium supplemented with heavy Lysine (L-Lysine-2HCL (13C6 99%, 15N2 99%) (Cambridge Isotope Laboratories, Inc.) (20mg/L ...
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bioRxiv - Immunology 2021Quote: CD138+ PCs were sorted on day 4 of in vitro differentiation and grown for 18 hrs in glucose free 1640 RPMI (Thermo) with dialyzed FBS supplemented with 2 g/L [U-13C6] glucose (Cambridge Isotope Laboratories). Metabolites were extracted and measured using Ultra High Performance Liquid Chromatography Mass Spectrometry (UHPLC-MS) ...
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bioRxiv - Biochemistry 2021Quote: ... 15N-labeled SARS-CoV-2 nsp7 sample was expressed and purified by the same methods as previously described in our NMR assignment paper.20 The recombinant Escherichia coli Rossetta2 pLysS was first grown in a M9 minimal medium containing 1 g/L 15N4HCl (Cambridge Isotope Laboratories), 4 g/L 13C6-D-Glucose (Cambridge Isotope Laboratories ...
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bioRxiv - Physiology 2024Quote: ... the cells were placed in 10 cm plates with media where the normal glucose was replaced with 13C6-L-glucose (Cambridge Isotope Laboratories). Cells were allowed to incubate in the 13C6-L-glucose prior to the in vitro ischemic-reperfusion injury (hypoxia/reoxygenation ...
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bioRxiv - Microbiology 2024Quote: ... 1 g/L of either (NH4)2SO4 (designated as 14N throughout the text) or (15NH4)2SO4 (designated as 15N, Cambridge Isotope Laboratories) was used as a source of nitrogen ...
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bioRxiv - Neuroscience 2020Quote: F2 SILAM wide type female mice were purchased from Cambridge Isotope Laboratories and then crossed with regular C57BL6 wild type males by fed with stable isotope labeling using amino acids in cell culture (SILAC) L-lysine (13C6) feed (Cambridge Isotope Laboratories, Inc). After 7 days ...
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bioRxiv - Molecular Biology 2021Quote: ... For the 15N isotope labeling cells were cultivated at 37 °C in M9 minimal medium with 1 g/L 15NH4Cl (Cambridge Isotope Laboratories, Inc.).
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bioRxiv - Biochemistry 2023Quote: Uniformly 15N labeled BTK samples were produced as described earlier by growth in modified M9 minimal media containing 15N ammonium chloride (1g/L, Cambridge Isotope Laboratories, Inc.) as the sole source of nitrogen [39] ...
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bioRxiv - Physiology 2023Quote: ... hearts were perfused for 20 minutes with a modified KH-buffer containing uniformly labelled glucose (5 mmol/L [U-13C]glucose, Cambridge Isotope Laboratories, Inc.) instead of standard glucose ...
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bioRxiv - Physiology 2024Quote: ... a baseline blood sample was drawn after which a primed constant infusion of L-[ring-13C6]-phenylalanine (0.05 µmol · kg -1 · min -1, prime 2 mol ·kg -1; Cambridge Isotope Laboratories, Danvers, MA) was initiated and maintained for the duration of the entire experiment (∼ 7 h) ...
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bioRxiv - Neuroscience 2020Quote: ... with a pH of 7.4 containing 6.15 mM NaN3 and 4.64 mM sodium 3-[trimethylsilyl] d4-propionate (Cambridge Isotope Laboratories) was prepared ...
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bioRxiv - Neuroscience 2023Quote: ... 5–10 μL of CSF or serum was extracted in 4:6:3 chloroform:methanol:water mixture supplemented with isotopically labeled T3 and T4 (at 100 nM, Cambridge Isotope Laboratories ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Samples were thawed on ice and phthalates were extracted from a 10 μL aliquot via vigorous vortexing for 30 min at 4°C in the presence of 5:3:2 methanol:acetonitrile:water (240 μL) containing an isotope labeled standard (1 μM DEHP ring-1,2-13C2, Cambridge Isotope Laboratories). Extraction supernatants were clarified via centrifugation at 18,000 rpm for 10 min at 4°C and analyzed immediately by ultra high-pressure liquid chromatography coupled to mass spectrometry (UHPLC-MS) ...
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bioRxiv - Biochemistry 2022Quote: ... a 100 mL LB starter culture treated with 100 μg/mL ampicillin was grown at 30 °C overnight and was used to inoculate 4× 1 L of M9 minimal medium containing 15NH4Cl and/or 13C6-glucose (Cambridge Isotope, Tewksbury, MA, USA). The cells were grown in this isotopically-enriched minimal media at 37 °C and shaken at 200 rpm until the OD600 reached 0.6-0.8 before a brief cold shock at 4 °C ...
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bioRxiv - Physiology 2021Quote: ... continuous infusion of stable amino acid isotope [13C6]-phenylalanine (L-phenylalanine, ring-13C6, 99%, CLM-1055-MPT; Cambridge Isotope Laboratories, Inc., Tewksbury, MA, USA) was used for measurement of amino acid kinetics across the limb and incorporation of labeled phenylalanine into muscle as previously described [20] ...
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bioRxiv - Biophysics 2024Quote: ... 15N isotope-labeled Ac-α-syn or α-syn were produced in M9 minimal medium with uniformly labeled 15NH4Cl (1 g/L, Cambridge Isotope Laboratories). Not isotope-labeled Ac-α-syn or α-syn were produced in LB medium ...
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Metabolic function in aging retina and retinal pigment epithelium remains robust despite vision lossbioRxiv - Biochemistry 2021Quote: Either 5 mM U-13C glucose alone or 5 mM unlabeled glucose and 2 mM U-13C glutamine U-13C metabolic tracers (99% isotopic purity) included 13C labeled D-Glucose and L-Glutamine (Cambridge Isotope Laboratories, Inc; Tewksbury, MA). Incubations ...
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bioRxiv - Cell Biology 2020Quote: ... 42 mg/L arginine hydrochloride (13C6, 15N4-arginine in ‘heavy’ media), and 146 mg/L lysine hydrochloride (13C6, 15N2-lysine in ‘heavy’ media) (Cambridge Isotope Laboratories, Tewksbury, MA, USA). Stable tetracycline-inducible cell line (FLAGOCIAD2 ...
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bioRxiv - Physiology 2022Quote: ... Three fish per tank per condition were injected with a 150 mM phenylalanine (PHE) solution containing 50% ring-[D5]-L-phenylalanine ([D5]-PHE, 98%, Cambridge Isotope Laboratories, Inc. Andover, USA) at a dose of 1 mL per 100 g of body mass and returned to their tanks for an incorporation period of three hours ...
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bioRxiv - Cancer Biology 2023Quote: ... Tracing of glutamine was performed by supplementing no-glucose and no-glutamine media with 4 mM 13C5 L-glutamine (Cambridge Isotope Laboratories, Inc., CLM-1822-H) and 5 mM unlabeled glucose ...
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bioRxiv - Developmental Biology 2022Quote: ... and L-Lysine (0.2 mM) were replaced with the non-radioactive isotopic forms Arg-10 (13C6 H14 15N4O2; Cambridge Isotope Laboratories, cat. no. CNLM-539-H-PK) and Lys-8 (13C6 H14 15N2O2 ...
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bioRxiv - Biophysics 2023Quote: ... They were then resuspended in 500 ml of M9 media supplemented with 100 mg of a desired 15N-labeled or 13C-labeled L-amino acid (Cambridge Isotope Labs, Inc. or Sigma-Aldrich, Inc.) as well as the remaining 19 unlabeled amino acids ...
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bioRxiv - Developmental Biology 2021Quote: ... Islets were then incubated on a rotator plate at 95 RPM for 90 minutes at 37°C and 5% CO2 before being transferred to Eppendorf tubes and the basal KRB exchanged for a 0.9 ml volume of KRB containing either 3 mM (low) or 17 mM (high) [U-13C6] glucose (Cambridge Isotope Laboratories, CLM 1396). Islets were then incubated for 1 hour at 37°C and 5% CO2 ...
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bioRxiv - Biochemistry 2022Quote: ... MMA quantification was performed using 3 uL of plasma that was spiked in with [U-13C]-MMA (Cambridge Isotope Laboratories, cat# CLM-9426-PK) as previously described (31 ...
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bioRxiv - Physiology 2024Quote: WT and Rnlsmut NIT-1 cells were seeded in the same experimental setup as untargeted analysis and maintained in DMEM supplemented with heavy labeled 5mM [13C6]-glucose for 24h (Cambridge Isotope Laboratories cat. 110187-42-3). Two 6-well plates of WT cells were treated with 5μM PG for the duration of the experiment ...