Labshake search
Citations for Cambridge Isotope Labs :
201 - 249 of 249 citations for 6 Chloro 4 hydroxy 3 methoxycarbonyl 2H thieno 2 3 e 1 2 thiazine 1 1 dioxide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: When the protein samples were prepared for NMR analysis the bacteria were cultured in M9 media containing D-[13C] glucose (U-13C6, 99%, CLM-1396-1, Cambridge Isotope) and 15NH4Cl (15NH4 Chloride ...
-
bioRxiv - Cancer Biology 2023Quote: ... Two transitions were optimised using an authentic standard of glycocholic acid (glycine-1-13C, CLM-191-PK, Cambridge Isotope Laobratories, UK), from the negative precursor ion (m/z 465 ...
-
bioRxiv - Biophysics 2024Quote: ... 4.0 gL-1 of D-glucose provided in specific isotopic ratios (12C, Merek, Germany; 13C, Cambridge Isotope Laboratories, USA; Table S1). 109 cells were harvested at late-exponential growth phase (Figure S3) ...
-
bioRxiv - Cancer Biology 2021Quote: ... cells were washed with 0.9% NaCl twice and lysed with pre-chilled extraction buffer that was composed of 480 ul of MeOH mixed with 120 ul of H2O and 1 uM of pre-mixed Heavy Amino Acid mix (Cambridge Isotope Laboratories). Lysates were transferred to an Eppendorf tube and vortexed at 4 °C for 10 min before being centrifuged at 16,000 g at 4 °C for 10 min ...
-
bioRxiv - Developmental Biology 2021Quote: ... /water (2:2:1) containing isotope-labeled internal standards ([U-13C6]glucose; [U-13C3]lactate; [U-13C5]glutamine; and [U-13C5]glutamate; Cambridge Isotope Laboratories) were spiked into media samples ...
-
bioRxiv - Cell Biology 2022Quote: ... Metabolites were extracted with 1 mL 80% MeOH pre-cooled to −80 °C containing 10 nmol [D8]-valine as an internal standard (Cambridge Isotope Labs). Insoluble material was removed by centrifugation at 21,000 ×g for 15 min at 4 °C ...
-
bioRxiv - Biochemistry 2021Quote: ... 15N-labeled SARS-CoV-2 nsp7 sample was expressed and purified by the same methods as previously described in our NMR assignment paper.20 The recombinant Escherichia coli Rossetta2 pLysS was first grown in a M9 minimal medium containing 1 g/L 15N4HCl (Cambridge Isotope Laboratories), 4 g/L 13C6-D-Glucose (Cambridge Isotope Laboratories ...
-
bioRxiv - Cancer Biology 2022Quote: ... H2O/ACN/MeOH supplemented with stable isotopic labeled amino acids solution at 1 nmol/mL (MSK-A2-1.2, Cambridge Isotope Laboratories, USA).
-
bioRxiv - Cancer Biology 2023Quote: ... Plates were then washed with PBS then switched to labeling media containing either 1 mM 13C5-glutamine (CLM-1822-H-PK, Cambridge Isotope Laboratories) or 5 mM 13C1,2-glucose (CLM-504-PK ...
-
bioRxiv - Epidemiology 2019Quote: ... using 90 μL of acetonitrile/methanol/formic acid (74.9:24.9:0.2 v/v/v) containing stable isotope-labeled internal standards (valine-d8, Sigma-Aldrich; St. Louis, MO; and phenylalanine-d8, Cambridge Isotope Laboratories; Andover, MA). The samples were centrifuged (10 min ...
-
bioRxiv - Cancer Biology 2022Quote: ... using 90 µL of acetonitrile/methanol/formic acid (74.9:24.9:0.2 v/v/v) containing stable isotope-labeled internal standards (valine-d8, Sigma-Aldrich; St. Louis, MO; and phenylalanine-d8, Cambridge Isotope Laboratories; Andover, MA). The samples were centrifuged (10 min ...
-
bioRxiv - Cancer Biology 2021Quote: ... activity in cultured melanoma cells was quantitated by measuring the transfer of 15N from [α–15N]glutamine (NLM-1016-1, Cambridge Isotope Laboratories) to 15NH4+ as described (37 ...
-
bioRxiv - Cancer Biology 2020Quote: ... Cell pellets were resuspended in 1 mL of 80% methanol solution containing 500 nM internal standards (Metabolomics Amino Acid Mix, Cambridge Isotope Laboratories Inc.). Samples were vortexed at 4 degrees ...
-
bioRxiv - Microbiology 2021Quote: ... The concentrations ranged from 0.1 to 1000 ng.mL-1 for each congener and 0.1 to 100 ng/ml for surrogates (Cambridge Isotope Labs Inc. Part# EDF-5524) (Table S3) ...
-
bioRxiv - Immunology 2020Quote: ... 25 μl of serum and 0.5 ml of Lipid Extraction Buffer containing 1 μg of D2-oleic acid (Cambridge isotope: DLM-689-0.1) and 1 μg of D14-palmitoleic acid (Cayman ...
-
bioRxiv - Molecular Biology 2021Quote: ... For the 15N isotope labeling cells were cultivated at 37 °C in M9 minimal medium with 1 g/L 15NH4Cl (Cambridge Isotope Laboratories, Inc.).
-
bioRxiv - Developmental Biology 2020Quote: Four aliquots of 200 μl of the bacterial suspension obtained as described above were added to 2 μl 10 mM 13C-citrulline (Lot No. PR-16536, Catalog No. CLM-4899-0, Cambridge Isotope Laboratories, Inc., Tewksbury, MA, USA). After 1 h of incubation ...
-
bioRxiv - Neuroscience 2023Quote: ... and heavy isotopes (4, 4, 5, 5-D4 L-lysine and 13C6 L-arginine, Cambridge Isotope Laboratories (CIL)) for the K4/R6 population ...
-
bioRxiv - Bioengineering 2022Quote: ... with 1 mL of dry-ice cold 80% methanol/20% water solution containing 1.5 μM Cambridge AA mix (Cambridge Isotope Laboratories, MSK-A2-1.2). Immediately after ...
-
bioRxiv - Cancer Biology 2023Quote: 5 μL of adult bovine serum or ftABSB was mixed with 45 μL of HPLC-grade methanol:water (80:20) including 500 nM 13C/15N labeled amino acids (Cambridge Isotope Laboratories, MSK-CAA-1). Samples were vortexed for 15 min at 4°C and insoluble material was sedimented by centrifugation at 16,000 x g for 10 min at 4°C ...
-
bioRxiv - Cancer Biology 2023Quote: ... 5 μL of media was mixed with 45 μL of HPLC-grade methanol:water (80:20) including 500 nM 13C/15N labeled amino acids (Cambridge Isotope Laboratories, MSK-CAA-1). Samples were then vortexed for 15 min at 4°C ...
-
bioRxiv - Biophysics 2024Quote: ... 15N isotope-labeled Ac-α-syn or α-syn were produced in M9 minimal medium with uniformly labeled 15NH4Cl (1 g/L, Cambridge Isotope Laboratories). Not isotope-labeled Ac-α-syn or α-syn were produced in LB medium ...
-
bioRxiv - Physiology 2023Quote: ... and [U-13C]-fructose-6-phosphate (M6-F6P) were purchased from Cambridge Isotope Labs (CLM-1553-PK ...
-
bioRxiv - Microbiology 2019Quote: ... Duplicate microcosms were fed with a 1 M solution of either 12C sodium butyrate or 13C-labeled sodium butyrate (>98% atom purity, Cambridge Isotope Laboratories, Tewksbury, MA, USA) to reach an initial butyrate concentration of 40 mM ...
-
bioRxiv - Microbiology 2021Quote: ... The sample was spiked with 10 pg.g-1 of an isotopically labelled surrogate compound mixture containing 13C labelled surrogates of the 17 congeners analyzed (Cambridge Isotope Laboratories Inc. Part # EDF 5393). The samples were extracted with toluene at 200°C and 2000 psi for 10 min (Dionex ASE 350) ...
-
bioRxiv - Biochemistry 2022Quote: Amino acids were quantified from 10 µL plasma precipitated with 180 µl ice-cold methanol containing 1 nmol of stable isotope labelled amino acids (Cambridge Isotope Laboratories, MSK-A2-1.2). Samples were incubated at -20°C for 30 min followed by centrifugation at 4°C (14,000g ...
-
bioRxiv - Biochemistry 2021Quote: ... and 0.798 mM L-(13C6,15N2)-lysine (Lys”8”)] or “medium” amino acids [0.398 mM L-(15N4)-arginine (Arg”4”) and 0.798 mM L-(D4)-lysine (Lys”4”)] (Cambridge Isotope Laboratories), and incubated for 2 hr as previously described elsewhere (Imami et al. ...
-
bioRxiv - Cancer Biology 2019Quote: ... which was supplemented with 25 mM 6-13C labeled D-glucose (Cambridge Isotope Laboratories). The cells were subjected to this treatment for 12.5 h ...
-
bioRxiv - Biophysics 2019Quote: ... except that 13C-D-glucose (Cambridge Isotope Laboratories; at 6 g/L of M9 media) was used as well ...
-
bioRxiv - Systems Biology 2023Quote: ... [U-13C]glucose-D-6-phosphate disodium salt hydrate (99%) were purchased from Cambridge Isotope Laboratories (Andover ...
-
bioRxiv - Biochemistry 2020Quote: ... and 0.798 mM L-(13C6,15N2)-lysine (Lys“8”)] or “medium” amino acids [0.398 mM L-(15N4)-arginine (Arg“4”) and 0.798 mM L-(D4)-lysine (Lys“4”)] (Cambridge Isotope Laboratories, Tewksbury, USA), and incubated for 2 h ...
-
bioRxiv - Biochemistry 2019Quote: ... and deuterium oxide (Cambridge Isotope Laboratories, DLM-4-1000). Protein expression was induced with 100 μM IPTG and cultures were grown for 18 hours at 18°C ...
-
bioRxiv - Biochemistry 2021Quote: ... 4 g/L 13C6-D-Glucose (Cambridge Isotope Laboratories) and 100 mg/L ampicillin at 37 °C ...
-
bioRxiv - Cancer Biology 2022Quote: ... or 4 mM U-[13C]-glutamine (Cambridge Isotope Laboratories) and 25 mM glucose (Sigma ...
-
bioRxiv - Bioengineering 2023Quote: ... 600 µl of D2O (Cambridge Isotope Laboratories (DLM-4)) was added and homogenized ...
-
bioRxiv - Cancer Biology 2023Quote: ... For the infusion of D2O (Cambridge Isotope, #DLM-4-50) and [2,2,3-2H]-serine (Cambridge Isotope ...
-
bioRxiv - Biophysics 2022Quote: ... 10% D2O (v/v) (Cambridge Isotope Laboratories; Cat. No. DLM-4-25) was added to the sample for the spectrometer deuterium lock ...
-
bioRxiv - Cancer Biology 2021Quote: ... 7.0 × 105 cells were plated onto 6 well plates for next day pulse-labeling with either 25 mM [1,2-13C] glucose (Cambridge Isotope Laboratories). Isotope labeling experiments were performed for 4 hours and extra plates were included for cell counts ...
-
bioRxiv - Genetics 2020Quote: ... injection of 4 mmol/kg of 15N-labeled ammonium chloride (15NH4Cl, Cambridge Isotope Laboratories) 20 min before terminal blood collection via cardiac puncture ...
-
bioRxiv - Molecular Biology 2020Quote: ... medium (M: R6K4 = +10Da) isotopes (13C on Arg, 4 D on Lys; Cambridge isotope laboratories ...
-
bioRxiv - Cancer Biology 2020Quote: ... 10 mM U-13C-glucose or 4 mM U-13C-glutamine (Cambridge Isotope Laboratories), which included control (DMSO ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... D23-dodecanoid acid and 13C6-4-hydroxybenzoic acid (98-99% enrichment, Cambridge Isotope Laboratories, Inc.) as well as a control with sterile oat/poultry-rearing pellet-powder ...
-
bioRxiv - Neuroscience 2022Quote: ... Catalog #:A456-4) prepared with Millipore water and spiked with deuterated isotope-labeled internal standards sourced from Cambridge Isotope Laboratories ...
-
bioRxiv - Biochemistry 2020Quote: ... with non-labeled Xyl6 (200 µg) and Ac-CoA (4 mM) in 100 mM potassium phosphate buffer in D2O (99.9%; Cambridge Isotope Laboratories) for 6 h at room temperature ...
-
bioRxiv - Cancer Biology 2022Quote: ... 4 weeks after induction tumor-bearing mice and healthy littermate controls were treated with 15N2-glutamine (Cambridge Isotope Laboratory #NLM-1328-PK) dissolved in sterile phosphate buffered saline at 7.2mg/animal by tail vein injection as previously described (Lane et al. ...
-
bioRxiv - Neuroscience 2023Quote: ... each rat was fed 1g of a pancake that contained 4 µl of [13C]-octanoic acid (Cambridge Isotope Laboratories, Inc, Tewksbury MA). After visually confirming the rats had eaten the entire pancake (in less than 5min or they were omitted from the study) ...
-
bioRxiv - Cancer Biology 2024Quote: ... the media was removed and replaced with glutamine-free media supplemented with 10% dFBS and 4 mmol/L U-13C5-L-glutamine (CLM-1822-H, Cambridge Isotope Laboratories) for 2 hours prior to harvest (n = 3 per condition) ...
-
bioRxiv - Immunology 2022Quote: ... BMDMs were infected by Mtb in DMEM medium supplemented with 10% dialyzed FBS and 4 mM of 50% U13C glutamine (Cambridge Isotope Laboratories, Inc, Tewksbury, MA) (2 mM labeled and 2 mM unlabeled glutamine ...
-
bioRxiv - Cancer Biology 2023Quote: ... Tracing of glutamine was performed by supplementing no-glucose and no-glutamine media with 4 mM 13C5 L-glutamine (Cambridge Isotope Laboratories, Inc., CLM-1822-H) and 5 mM unlabeled glucose ...