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Citations for Cambridge Isotope Labs :
151 - 193 of 193 citations for FMOC L MEORN MTR OH since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... and proteins were produced in the M9 media supplemented with 2 g/L [15N]NH4Cl (Cambridge Isotope Laboratories) and 3 g/L d-[13C6]glucose (Cambridge Isotope Laboratories) ...
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bioRxiv - Biochemistry 2021Quote: ... Uniformly 15N-labeled CaM was prepared in M9 minimal medium containing 0.5 g/L 15NH4Cl (Cambridge Isotope Laboratories) as the sole nitrogen source.
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bioRxiv - Physiology 2020Quote: ... constant infusion (0.0085 mg•kg-1•min-1) of [ring-13C6]-L-phenylalanine (Cambridge Isotope Laboratories, Tewksbury, MA) from 1000 to the end of the protocol ...
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bioRxiv - Biophysics 2022Quote: ... and 2 g/L U-[2H] glucose (Sigma-Aldrich, St. Louis, MO/Cambridge Isotope Laboratories, Inc., Tewksbury, MA). Cells were grown up to an OD600 of 0.65 at 37 °C ...
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bioRxiv - Biochemistry 2024Quote: ... Heavy arginine and lysine contain the isotopes 13C and 15N: L-lysine:2HCl (Cambridge Isotope #CNLM-291-H) and L-arginine:HCl (Cambridge isotope # CNLM-539-H).
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bioRxiv - Biophysics 2023Quote: ... sydowii was grown in 100 mL of liquid media containing 20 g/L 13C-glucose (Cambridge Isotope Laboratories) and 2 g/L 15N-labeled NH4NO3 (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... 13C5-L-Glutamine (Cat#CLM-1822-H) and 13C2-Sodium acetate (Cat#CLM-440-1) from Cambridge Isotope Laboratories ...
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bioRxiv - Microbiology 2023Quote: ... glycine (2-13C, 99%; 15N, 98%+), L-phenylalanine (D8, 98%), or tyramine:HCl (1,1,2,2-D4, 98%) (Cambridge Isotope Laboratories, Inc.) was added to BHIS containing 0.1% (w/v ...
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bioRxiv - Cancer Biology 2022Quote: ... Dried pellet from unlabeled samples was mixed with [U13C]-D/L-2HG (internal standard for unlabeled samples, Cambridge isotope laboratories ...
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bioRxiv - Immunology 2021Quote: ... cells were transferred to serine/glycine free media containing 13C-U-glucose (2g/L) (Cambridge Isotope Laboratories, CLM-1396-PK) for additional 2 hours ...
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bioRxiv - Biophysics 2024Quote: ... 1 L cultures of autoinduction media (Cold Spring Harbor Protocols) were used with 15N labeled ammonium chloride (Cambridge Isotope Laboratories). The final antibiotic concentration was increased to 0.1 mg/mL of kanamycin to reduce plasmid loss and poor yields ...
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bioRxiv - Immunology 2023Quote: 2×106 BMDCs were treated with 1mM uniformly labeled 13C lactate (L-LA*) (CLM-1579-N-0.1MG, Cambridge Isotope Laboratories), in the presence or absence of LPS (100ng/mL) ...
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bioRxiv - Neuroscience 2024Quote: ... On day 8, mice were switched to Mouse Express L-Lysine (13C6, 99%) irradiated mouse feed (Cambridge Isotope Laboratories, USA) for 8 days at libitum ...
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bioRxiv - Neuroscience 2024Quote: ... Heavy SILAC media was supplemented with L-Lysine:2HCl (13C6, 99%; 15N2, 99%) (Cambridge Isotope Laboratories, Inc., CNLM-291-H) and L-Arginine:HCl (13C6 ...
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bioRxiv - Biophysics 2021Quote: ... The cells were collected by centrifugation and resuspended in M9 medium (1 L, 100% D2O) supplemented with 15NH4Cl (Cambridge Isotope Laboratories) and 12C6H12O6 as the sole nitrogen and carbon sources ...
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bioRxiv - Molecular Biology 2020Quote: ... cultures were first grown at 37°C in 1 L of LB media until an OD600 of 0.6 before the cells were pelleted and resuspended in 0.5 L M9 minimal media prepared in D2O supplemented with 13C-D-glucose and 15NH4Cl (Cambridge Isotope Laboratories). After 1 hour recovery at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... A pulse of 0.025% (w/v) stable isotope-labeled lysine derivative 13C615N2 L-lysine (“heavy” lysine, Lys8, K8, Cambridge Isotope Laboratories) was then added in the presence of ampicillin and 1 – 2 mL of cultures were harvested in intermittent time intervals (10min - 24h ...
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bioRxiv - Microbiology 2023Quote: ... was pipetted into 1.7 mL tubes and was then layered with 150 µL of 13C5 15N2- L-Gln (Cambridge Isotope Laboratories) to yield a final concentration of 50 µM when mixed with cells ...
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bioRxiv - Cancer Biology 2023Quote: ... U-[13C]-Q labelling media consisted of DMEM supplemented with 2 mM L-glutamine (U-13C5; CLM-1822; Cambridge Isotope Laboratories), 10 mM glucose ...
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bioRxiv - Biochemistry 2022Quote: ... #CLM-893), and L-tryptophan (13C11, 99%; #CLM-4290) and tracer glucose (13C6, 99%, #CLM-1396) were obtained from Cambridge Isotope Laboratories ...
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bioRxiv - Pathology 2024Quote: ... dissolved in sterile PBS or 0.5 mg/gram of body weight 13C3 Sodium L-lactate (CLM-1579-0.5, 20% w/w in water, Cambridge Isotope Laboratories). Twenty minutes after injection mice were euthanized via exsanguination by cardiac puncture under non-recovery isoflurane anesthesia ...
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bioRxiv - Immunology 2021Quote: CD138+ PCs were sorted on day 4 of in vitro differentiation and grown for 18 hrs in glucose free 1640 RPMI (Thermo) with dialyzed FBS supplemented with 2 g/L [U-13C6] glucose (Cambridge Isotope Laboratories). Metabolites were extracted and measured using Ultra High Performance Liquid Chromatography Mass Spectrometry (UHPLC-MS) ...
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bioRxiv - Biochemistry 2021Quote: ... 15N-labeled SARS-CoV-2 nsp7 sample was expressed and purified by the same methods as previously described in our NMR assignment paper.20 The recombinant Escherichia coli Rossetta2 pLysS was first grown in a M9 minimal medium containing 1 g/L 15N4HCl (Cambridge Isotope Laboratories), 4 g/L 13C6-D-Glucose (Cambridge Isotope Laboratories ...
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bioRxiv - Physiology 2024Quote: ... the cells were placed in 10 cm plates with media where the normal glucose was replaced with 13C6-L-glucose (Cambridge Isotope Laboratories). Cells were allowed to incubate in the 13C6-L-glucose prior to the in vitro ischemic-reperfusion injury (hypoxia/reoxygenation ...
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bioRxiv - Biochemistry 2023Quote: ... 15N-labeled protein samples were grown in 1L minimal media containing 3 g/L 15N ammonium chloride (Cambridge Isotope Laboratories, Tweksbury, MA) and unlabeled protein samples were grown in 1L super broth media ...
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bioRxiv - Biochemistry 2024Quote: ... the media was supplemented with: 1) 50 mg/L 2-keto-3-[D2],4-[13C]-butyrate (Cambridge Isotope Laboratories, Inc. CDLM-7318) and 100 mg/L 2-keto-3-[D]-[13CH3,12CD3]- isovalerate (Cambridge Isotope Laboratories ...
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bioRxiv - Microbiology 2024Quote: ... 1 g/L of either (NH4)2SO4 (designated as 14N throughout the text) or (15NH4)2SO4 (designated as 15N, Cambridge Isotope Laboratories) was used as a source of nitrogen ...
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bioRxiv - Neuroscience 2020Quote: F2 SILAM wide type female mice were purchased from Cambridge Isotope Laboratories and then crossed with regular C57BL6 wild type males by fed with stable isotope labeling using amino acids in cell culture (SILAC) L-lysine (13C6) feed (Cambridge Isotope Laboratories, Inc). After 7 days ...
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bioRxiv - Molecular Biology 2021Quote: ... For the 15N isotope labeling cells were cultivated at 37 °C in M9 minimal medium with 1 g/L 15NH4Cl (Cambridge Isotope Laboratories, Inc.).
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bioRxiv - Biophysics 2024Quote: ... The cells were used to inoculate 2L of H2O-based M9 media supplemented with 3 g L-1 of [U-13C6] d-glucose (Cambridge Isotope Laboratories, CIL) and 2 g L-1 of [15N] ammonium chloride (Cambridge Isotope Laboratories ...
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bioRxiv - Physiology 2023Quote: ... hearts were perfused for 20 minutes with a modified KH-buffer containing uniformly labelled glucose (5 mmol/L [U-13C]glucose, Cambridge Isotope Laboratories, Inc.) instead of standard glucose ...
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bioRxiv - Physiology 2024Quote: ... a baseline blood sample was drawn after which a primed constant infusion of L-[ring-13C6]-phenylalanine (0.05 µmol · kg -1 · min -1, prime 2 mol ·kg -1; Cambridge Isotope Laboratories, Danvers, MA) was initiated and maintained for the duration of the entire experiment (∼ 7 h) ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were cultured in glucose and glutamine-free RPMI-1640 media supplemented with 1 mM labeled 13C5-L-Glutamine (Cambridge Isotope Laboratories Inc.), 10 mM unlabeled Glucose for 16 hours in triplicate ...
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bioRxiv - Biochemistry 2022Quote: ... a 100 mL LB starter culture treated with 100 μg/mL ampicillin was grown at 30 °C overnight and was used to inoculate 4× 1 L of M9 minimal medium containing 15NH4Cl and/or 13C6-glucose (Cambridge Isotope, Tewksbury, MA, USA). The cells were grown in this isotopically-enriched minimal media at 37 °C and shaken at 200 rpm until the OD600 reached 0.6-0.8 before a brief cold shock at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... 500 μl of each cell lysate sample was blown to dryness with N2 and reconstituted with 150 μL of an Acetonitrile/H2O (2:1) solution containing stable isotope-labeled internal standards: tyrosine-d2 and lactate-13C3 (Cambridge Isotope Lab, Tewksbury, MA). Centrifuged the cell lysate at 10,000 g for 20 minutes ...
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bioRxiv - Physiology 2021Quote: ... continuous infusion of stable amino acid isotope [13C6]-phenylalanine (L-phenylalanine, ring-13C6, 99%, CLM-1055-MPT; Cambridge Isotope Laboratories, Inc., Tewksbury, MA, USA) was used for measurement of amino acid kinetics across the limb and incorporation of labeled phenylalanine into muscle as previously described [20] ...
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bioRxiv - Microbiology 2024Quote: ... with and without supplementation with 1 mM of stable-isotope enriched 13C L-DOPA (RING-13C6, 99%, CLM-1007-0, Cambridge Isotope Laboratories, MA, USA) for 7 d ...
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Metabolic function in aging retina and retinal pigment epithelium remains robust despite vision lossbioRxiv - Biochemistry 2021Quote: Either 5 mM U-13C glucose alone or 5 mM unlabeled glucose and 2 mM U-13C glutamine U-13C metabolic tracers (99% isotopic purity) included 13C labeled D-Glucose and L-Glutamine (Cambridge Isotope Laboratories, Inc; Tewksbury, MA). Incubations ...
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bioRxiv - Cell Biology 2020Quote: ... 42 mg/L arginine hydrochloride (13C6, 15N4-arginine in ‘heavy’ media), and 146 mg/L lysine hydrochloride (13C6, 15N2-lysine in ‘heavy’ media) (Cambridge Isotope Laboratories, Tewksbury, MA, USA). Stable tetracycline-inducible cell line (FLAGOCIAD2 ...
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bioRxiv - Physiology 2022Quote: ... Three fish per tank per condition were injected with a 150 mM phenylalanine (PHE) solution containing 50% ring-[D5]-L-phenylalanine ([D5]-PHE, 98%, Cambridge Isotope Laboratories, Inc. Andover, USA) at a dose of 1 mL per 100 g of body mass and returned to their tanks for an incorporation period of three hours ...
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bioRxiv - Cancer Biology 2023Quote: ... Tracing of glutamine was performed by supplementing no-glucose and no-glutamine media with 4 mM 13C5 L-glutamine (Cambridge Isotope Laboratories, Inc., CLM-1822-H) and 5 mM unlabeled glucose ...
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bioRxiv - Developmental Biology 2022Quote: ... and L-Lysine (0.2 mM) were replaced with the non-radioactive isotopic forms Arg-10 (13C6 H14 15N4O2; Cambridge Isotope Laboratories, cat. no. CNLM-539-H-PK) and Lys-8 (13C6 H14 15N2O2 ...
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bioRxiv - Biophysics 2023Quote: ... They were then resuspended in 500 ml of M9 media supplemented with 100 mg of a desired 15N-labeled or 13C-labeled L-amino acid (Cambridge Isotope Labs, Inc. or Sigma-Aldrich, Inc.) as well as the remaining 19 unlabeled amino acids ...