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Citations for Cambridge Isotope Labs :
151 - 179 of 179 citations for FITC labeled Fibrinogen since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... The tissue powder was then weighed into pre-chilled tubes and extracted with methanol (containing 500 nM each of 17 isotopically labeled 13C/15N amino acids (Cambridge Isotope Laboratories, Inc.)) ...
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bioRxiv - Cancer Biology 2022Quote: ... the cell pellet was resuspended into 1 mL of ice-cold 2:2:1 MeOH: ACN: H2O (v/v/v) containing 10 μM stable isotope-labeled canonical amino acid mix (Cambridge Isotope Laboratories, Inc) as internal standards ...
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bioRxiv - Cell Biology 2024Quote: PASMCs were seeded into a 6-well plate and labeled in complete fresh SmBM-2 medium supplemented with 1 mM uniformly [13C]-labeled KIC (13C6-KIC) isotope (cat # CLM-4785-0.1, Cambridge Isotope Laboratories; Tewksbury, MA) for 8 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were cultured in glucose- and glutamine-free RPMI-1640 media supplemented with 500 µM labeled 13C2-acetate (Cambridge Isotope Laboratories Inc.), 10 mM unlabeled Glucose for 16 hours in triplicate ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were cultured in glucose and glutamine-free RPMI-1640 media supplemented with 1 mM labeled 13C5-L-Glutamine (Cambridge Isotope Laboratories Inc.), 10 mM unlabeled Glucose for 16 hours in triplicate ...
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bioRxiv - Biophysics 2022Quote: ... then centrifuging and resuspending in M9 minimal medium containing 2 g/L unlabeled glucose and 1 g/L 15N-labeled ammonium acetate (Cambridge Isotope Labs, Tewksbury, MA) and induced with 1 mM IPTG ...
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bioRxiv - Cancer Biology 2022Quote: ... Dried samples were rehydrated with 2:1 methanol:water into which a mixture of isotopically labeled amino acids of known concentrations (Cambridge Isotope Laboratories, MSK-A2-1.2) was added ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... were diluted using 90 µL of 74.9:24.9:0.2 v/v/v acetonitrile/methanol/formic acid containing stable isotope-labeled internal standards (valine-d8, Isotec; and phenylalanine-d8, Cambridge Isotope Laboratories; Andover, MA). The samples were centrifuged (10 min ...
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bioRxiv - Cell Biology 2020Quote: ... 500 μl of each cell lysate sample was blown to dryness with N2 and reconstituted with 150 μL of an Acetonitrile/H2O (2:1) solution containing stable isotope-labeled internal standards: tyrosine-d2 and lactate-13C3 (Cambridge Isotope Lab, Tewksbury, MA). Centrifuged the cell lysate at 10,000 g for 20 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... cells while expression was carried out in LB media for non-labeled protein and M9 minimal media containing 15NH4Cl (Cambridge Isotope Laboratories, Tewksbury, MA) for 15N ...
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bioRxiv - Cancer Biology 2023Quote: 5 μL of adult bovine serum or ftABSB was mixed with 45 μL of HPLC-grade methanol:water (80:20) including 500 nM 13C/15N labeled amino acids (Cambridge Isotope Laboratories, MSK-CAA-1). Samples were vortexed for 15 min at 4°C and insoluble material was sedimented by centrifugation at 16,000 x g for 10 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... L-LYSINE:2HCL (13C6, 99%;15N2, 99%) and stable isotope-labeled (heavy) L-ARGININE:HCL (13C6, 99%; 15N4, 99%) (Cambridge Isotope Labs, Inc. Andover, MA). Light SILAC medium was generated by adding unlabeled L-LYSINE:2HCL (final concentration 146.0 mg/mL ...
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bioRxiv - Cancer Biology 2023Quote: 100 µL of each CSF sample was spiked with 5 µL of a mixture of stable isotope-labeled standards (Cambridge Isotope Labs, Tewksbury, MA). An aliquot of pre-chilled methanol was added to each sample for a final composition of 80% methanol to extract metabolites and precipitate proteins ...
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bioRxiv - Cell Biology 2023Quote: ... Taurine concentrations were then measured via LC-MS and analyzed the area of the exact mass for the corresponding ions of the targets as well as the intracellular labeled isotopic taurine levels (13C2, 99%; 15N, 98%, Cambridge isotope #CNLM-10253-PK).
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bioRxiv - Cancer Biology 2023Quote: ... 5 μL of media was mixed with 45 μL of HPLC-grade methanol:water (80:20) including 500 nM 13C/15N labeled amino acids (Cambridge Isotope Laboratories, MSK-CAA-1). Samples were then vortexed for 15 min at 4°C ...
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Metabolic function in aging retina and retinal pigment epithelium remains robust despite vision lossbioRxiv - Biochemistry 2021Quote: Either 5 mM U-13C glucose alone or 5 mM unlabeled glucose and 2 mM U-13C glutamine U-13C metabolic tracers (99% isotopic purity) included 13C labeled D-Glucose and L-Glutamine (Cambridge Isotope Laboratories, Inc; Tewksbury, MA). Incubations ...
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bioRxiv - Microbiology 2022Quote: ... were resuspended in 300 μL of LC/MS grade methanol containing a mixture of 17 isotope-labeled amino acids (200 nM, Cambridge Isotope Laboratories, MSK-A2-1.2). To this solution ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 µL of urine was mixed with 90 µL of methanol: water (1:1) and internal standards (Labeled tryptophan (CNLM-2475-H-PK, Cambridge Isotope Laboratories, Inc., Andover, MA), zeatine and inosine ...
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bioRxiv - Cancer Biology 2022Quote: ... using 90 µL of acetonitrile/methanol/formic acid (74.9:24.9:0.2 v/v/v) containing stable isotope-labeled internal standards (valine-d8, Sigma-Aldrich; St. Louis, MO; and phenylalanine-d8, Cambridge Isotope Laboratories; Andover, MA). The samples were centrifuged (10 min ...
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bioRxiv - Cell Biology 2022Quote: ... Ultra pure bovine serum albumin (Cell Signalling Technology 9998) was used as a protein source and iPSDM were incubated with media containing 13C-labeled glucose (Cambridge Isotope Laboratories CLM-1396-5) and the described treatments for 1h before continuing with downstream sample processing ...
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bioRxiv - Genomics 2023Quote: ... followed by metabolite extraction in ice cold 80:20 LC/MS grade methanol: water buffer containing 15N and 13C fully-labeled amino acid standards (Cambridge Isotope Laboratories #MSK-A2-1.2). Samples were vortexed for 10 minutes at 4°C and then centrifuged at maximum speed (> 14,000g ...
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bioRxiv - Cell Biology 2022Quote: ... Polar metabolites were extracted in ice cold 80% LC/MS grade methanol containing 15N and 13C fully-labeled amino acid standards (Cambridge Isotope Laboratories #MSK-A2-1.2). Tissues were homogenized by Bead Ruptor 24 (Omni International ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed twice with ice-cold 0.9% NaCl and polar metabolites extracted in ice cold 80% LC/MS grade methanol containing 15N and 13C fully-labeled amino acid standards (Cambridge Isotope Laboratories #MSK-A2-1.2). Extracts were vigorously shaken by vortex for 10 minutes at 4°C and spun at 14,000 rpm at 4°C for 10 minutes to remove insoluble cell debris ...
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bioRxiv - Neuroscience 2023Quote: ... neurons were gently washed with PBS twice and switched into media containing the same components except for replacing light lysine with heavy stable isotope labeled (13C615N2) lysine (Cambridge Isotope Laboratories #CNLM-291-H-PK). For multiple time point experiments ...
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bioRxiv - Microbiology 2022Quote: ... DNA with different levels of 13C enrichment was produced by varying the ratio of unlabeled glucose to uniformly-labeled 13C6-D-glucose (Cambridge Isotope Laboratories; CLM-1396; 99 atom %), e.g ...
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bioRxiv - Physiology 2024Quote: WT and Rnlsmut NIT-1 cells were seeded in the same experimental setup as untargeted analysis and maintained in DMEM supplemented with heavy labeled 5mM [13C6]-glucose for 24h (Cambridge Isotope Laboratories cat. 110187-42-3). Two 6-well plates of WT cells were treated with 5μM PG for the duration of the experiment ...
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bioRxiv - Plant Biology 2024Quote: ... The 15N-labeled medium contained 1g/L 15NH4Cl (≥98 atom % 15N) and 1g/L of Celtone® Base Powder (≥98% 15N) (Cambridge Isotope Laboratories, CGM-1030P-N). Both media pH were 7 ...
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bioRxiv - Cell Biology 2024Quote: ... LysoIP and MockIP samples were resuspended in LC-MS grade 80% methanol (v/v) with isotopically labeled amino acids (Cambridge Isotope Laboratories, Inc. Catalog #MSK-A2-1.2). Metabolites were profiled by liquid chromatography/mass spectrometry (LC/MS ...
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bioRxiv - Systems Biology 2024Quote: ... 10 µL of spent media samples were mixed with 10 µL of known concentration of isotopically labeled internal standards of amino acids (Cambridge Isotope Laboratory, Andover, MA, MSK-A2-1.2). 600 µL of ice cold HPLC grade methanol was added ...