Labshake search
Citations for Cambridge Isotope Labs :
151 - 200 of 324 citations for 6 Hydroxy 2 oxo 2H 1 benzopyran 3 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2024Quote: ... [15N]5-2-deoxyadenosine was obtained from Cambridge Isotope Laboratories ...
-
bioRxiv - Physiology 2022Quote: ... ice-cold 400μL saline and ice-cold dH2O containing amino acid isotope labelled internal standards (Cambridge Isotope Laboratories, #MSK-A2-1.2). An aliquot of homogenate (50μL ...
-
bioRxiv - Biochemistry 2020Quote: An internal standard stock mixture was prepared using the following compounds and concentrations: citric acid-d4 (Cambridge Isotope Laboratories, DLM-3487), 225 μM ...
-
bioRxiv - Cell Biology 2021Quote: ... and then harvested with 1mL/well of 80% methanol (on dry ice) with 500 nM 13C- and 15N-labeled amino acids (Cat#MSK-A1-1.2, Cambridge Isotope Laboratories) as internal standards ...
-
bioRxiv - Neuroscience 2020Quote: ... and SILAC amino acids (13C6-15N2-lysine, 13C6-15N4-arginine (Lys8/Arg10) and D4-lysine/13C6-arginine (Lys4/Arg6)) were obtained from Cambridge Isotope Laboratories ...
-
bioRxiv - Cancer Biology 2022Quote: ... and SVVESVKFL+7 were synthesized at the MIT-Koch Institute Swanson Biotechnology Center in Biopolymers and Proteomics Facility using standard Fmoc chemistry using an Intavis model MultiPep peptide synthesizer with HATU activation and 5 μmol chemistry cycles as previously described.(24) Standard Fmoc amino acids were procured from NovaBiochem and Fmoc-Leu (13C6, 15N) was obtained from Cambridge Isotope Laboratories ...
-
bioRxiv - Cell Biology 2021Quote: ... that cannot form lysine-linked polySUMO chains were grown for at least ten divisions in synthetic complete media supplemented either with unlabeled (Lys0 and Arg0; light) or heavy isotope-labeled amino acids (Lys8 and Arg10; heavy) from Cambridge Isotope Laboratories ...
-
bioRxiv - Molecular Biology 2022Quote: ... medium (L-arginine:HCL [U13C6] and L-lysine:2HCL [4,4,5,5-D4], or heavy (L-arginine:HCL (13C6, 15N4) and L-lysine:2HCL (13C6, 15N2)) amino acid isotopes (Cambridge Isotope Laboratories Inc.).
-
bioRxiv - Cell Biology 2023Quote: ... of 80% methanol in LC–MS water containing 500 nM isotope-labelled amino acids used as internal standards (Cambridge Isotope Laboratories). Following extraction ...
-
bioRxiv - Biochemistry 2023Quote: RPE cells were incubated in pH 7.4 α-MEM and 50 µM [U-13C16]-palmitic acid (Cambridge isotope labs, CLM-409) complexed to bovine serum albumin at a 6:1 ratio of fatty acid to albumin ...
-
bioRxiv - Neuroscience 2023Quote: ... Chromatographic performance was quality controlled using a mixture of unlabeled standard amino acids and a mixture of chemically diverse compounds (Cambridge Isotope Laboratories ...
-
bioRxiv - Molecular Biology 2023Quote: ... the cells were washed three times with 1 ml of cold 0.9% NaCl and scraped into 500µL of cold 80%v/v LC-MS grade methanol containing heavy amino acid internal standards (Cambridge Isotope Laboratories). After 10 minutes of vortexing at 4°C ...
-
bioRxiv - Cell Biology 2024Quote: ... SRM transitions were detailed in the TraceFinder software and then verified with the Metabolomics Amino Acid Mix Standard (Cambridge Isotope Laboratories) for unique transitions ...
-
bioRxiv - Biochemistry 2024Quote: ... and 2 g/L U-13C6-Glucose (Cambridge Isotope Laboratories) as the sole nitrogen and carbon sources ...
-
bioRxiv - Cell Biology 2020Quote: ... L amino acids were substituted for the same concentrations of heavy isotope–labeled (H) amino acids 13C6-15N2 L-Lys:2HCl and 13C6-15N4 L-Arg (Cambridge Isotope Laboratories). For immature dendritic cells (Dc ...
-
bioRxiv - Cell Biology 2020Quote: Cells were washed twice with ice cold PBS and extracted on dry-ice in 0.8 mL 80% methanol containing 500 nM internal standards (Metabolomics Amino Acid Mix Standard: Cambridge Isotope Laboratories, Inc.). Cell extracts were collected using a cell scraper and transferred to a microcentrifuge tube ...
-
bioRxiv - Cell Biology 2022Quote: ... Polar metabolites were extracted in 80 % methanol containing 15N and 13C fully-labeled amino acid standards (MSK-A2-1.2, Cambridge Isotope Laboratories, Inc). Extracts were shaken for 10 min with a vortexer ...
-
bioRxiv - Cell Biology 2021Quote: ... and 500 μl of ice-cold 80% methanol in water with 250nM 13C/15N labeled amino acid standards (MSK-A2-1.2: Cambridge Isotope Laboratories, Inc.) was added to each well ...
-
bioRxiv - Biochemistry 2020Quote: ... and 0.798 mM L-(13C6,15N2)-lysine (Lys“8”)] or “medium” amino acids [0.398 mM L-(15N4)-arginine (Arg“4”) and 0.798 mM L-(D4)-lysine (Lys“4”)] (Cambridge Isotope Laboratories, Tewksbury, USA), and incubated for 2 h ...
-
bioRxiv - Cell Biology 2021Quote: ... which were replaced with either unlabelled amino-acids (Lys0 and Arg0) or stable isotopic forms 13C6 15N2-lysine (Lys8) and 13C6 15N4-arginine (Arg10) (Cambridge Isotope Laboratories). The medium was supplemented with 10% dialyzed FBS using previous published methods (Golebiowski et al ...
-
bioRxiv - Microbiology 2021Quote: ... 2 mL of LC-MS grade water containing 0.1 μL of MSK-A2-1.2 stable isotope-labeled amino acid standards (Cambridge Isotope Laboratories, Tewksbury, MA) was added before vortexing for 1 minute ...
-
bioRxiv - Biochemistry 2021Quote: ... then 10% of bead volume was lysed with 1% Triton buffer for protein extracts and the remaining 90% was extracted in 80% methanol containing heavy labeled amino acid standards (Cambridge Isotope Labs) on a rotator shaker for 10□min at 4□°C.
-
bioRxiv - Neuroscience 2023Quote: ... each rat was fed 1g of a pancake that contained 4 µl of [13C]-octanoic acid (Cambridge Isotope Laboratories, Inc, Tewksbury MA). After visually confirming the rats had eaten the entire pancake (in less than 5min or they were omitted from the study) ...
-
bioRxiv - Cell Biology 2023Quote: ... and either “Heavy” amino acids (50 μg/ml 13C6, 15N4 Arginine HCl and 100 μg/ml 13C6, 15N2 Lysine 2HCl; Cambridge Isotope Laboratories), or the equivalent “Light” amino acids ...
-
bioRxiv - Cancer Biology 2023Quote: ... Media was aspirated from cells and then washed rapidly in ice cold bank saline followed by addition of 500 mL ice-cold 80% methanol in water containing 500 nM each of 17 isotopically labeled 13C/15N amino acids (Cambridge Isotope Laboratories). Alternatively ...
-
Sex-specific fear acquisition following early life stress is linked to amygdala glutamate metabolismbioRxiv - Animal Behavior and Cognition 2024Quote: ... U-13C15N-labeled amino acids at a final concentration of 0.25 µM (prepared as 2.5 mM in 0.1 N HCl, Cambridge Isotope Laboratories, MSK-A2-1.2), citric acid d4 at 0.02 µg/mL (dissolved at 100 µg/mL in H2O ...
-
bioRxiv - Cancer Biology 2021Quote: ... 7.0 × 105 cells were plated onto 6 well plates for next day pulse-labeling with either 25 mM [1,2-13C] glucose (Cambridge Isotope Laboratories). Isotope labeling experiments were performed for 4 hours and extra plates were included for cell counts ...
-
bioRxiv - Cancer Biology 2020Quote: ... 10μL of media was mixed with 10μL of a mixture of isotopically labeled metabolites and amino acids of known concentrations (Cambridge Isotope Laboratories, Tewksbury, MA) and extracted with 600μL ice-cold HPLC grade methanol ...
-
bioRxiv - Neuroscience 2020Quote: F2 SILAM wide type female mice were purchased from Cambridge Isotope Laboratories and then crossed with regular C57BL6 wild type males by fed with stable isotope labeling using amino acids in cell culture (SILAC) L-lysine (13C6) feed (Cambridge Isotope Laboratories, Inc). After 7 days ...
-
bioRxiv - Microbiology 2023Quote: ... were prepared via protein precipitation with the addition of 9 volumes of 74.9:24.9:0.2 v/v/v acetonitrile/methanol/formic acid containing stable isotope-labeled internal standards (valine-d8, Sigma-Aldrich; and phenylalanine-d8, Cambridge Isotope Laboratories). The samples were centrifuged (10 minutes ...
-
bioRxiv - Cancer Biology 2023Quote: ... The tissue powder was then weighed into pre-chilled tubes and extracted with methanol (containing 500 nM each of 17 isotopically labeled 13C/15N amino acids (Cambridge Isotope Laboratories, Inc.)) ...
-
bioRxiv - Bioengineering 2023Quote: ... supplemented with 2 g / L 13C6–D-glucose (Cambridge Isotope Laboratories) and sodium bicarbonate (Sigma Aldrich) ...
-
bioRxiv - Animal Behavior and Cognition 2022Quote: ... were diluted using 90 µL of 74.9:24.9:0.2 v/v/v acetonitrile/methanol/formic acid containing stable isotope-labeled internal standards (valine-d8, Isotec; and phenylalanine-d8, Cambridge Isotope Laboratories; Andover, MA). The samples were centrifuged (10 min ...
-
bioRxiv - Physiology 2021Quote: ... continuous infusion of stable amino acid isotope [13C6]-phenylalanine (L-phenylalanine, ring-13C6, 99%, CLM-1055-MPT; Cambridge Isotope Laboratories, Inc., Tewksbury, MA, USA) was used for measurement of amino acid kinetics across the limb and incorporation of labeled phenylalanine into muscle as previously described [20] ...
-
bioRxiv - Biochemistry 2021Quote: ... Tissue was incubated in Krebs-Ringer buffer (formulations used in each figure specified below below) supplemented with 5 mM glucose and [U-13C]-succinic acid (Cambridge isotope CLM-1571-0.1) or [U-13C]-malate (Cambridge isotope CLM-8065 ...
-
bioRxiv - Microbiology 2022Quote: ... supplemented with 10 mM 13C6-glucose (Cambridge Isotope Laboratories, CLM-1396-2) and dialyzed FBS (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2022Quote: ... were resuspended in 300 μL of LC/MS grade methanol containing a mixture of 17 isotope-labeled amino acids (200 nM, Cambridge Isotope Laboratories, MSK-A2-1.2). To this solution ...
-
bioRxiv - Cell Biology 2022Quote: ... Polar metabolites were extracted in ice cold 80% LC/MS grade methanol containing 15N and 13C fully-labeled amino acid standards (Cambridge Isotope Laboratories #MSK-A2-1.2). Tissues were homogenized by Bead Ruptor 24 (Omni International ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were washed twice with ice-cold 0.9% NaCl and polar metabolites extracted in ice cold 80% LC/MS grade methanol containing 15N and 13C fully-labeled amino acid standards (Cambridge Isotope Laboratories #MSK-A2-1.2). Extracts were vigorously shaken by vortex for 10 minutes at 4°C and spun at 14,000 rpm at 4°C for 10 minutes to remove insoluble cell debris ...
-
bioRxiv - Genomics 2023Quote: ... followed by metabolite extraction in ice cold 80:20 LC/MS grade methanol: water buffer containing 15N and 13C fully-labeled amino acid standards (Cambridge Isotope Laboratories #MSK-A2-1.2). Samples were vortexed for 10 minutes at 4°C and then centrifuged at maximum speed (> 14,000g ...
-
Multi-omics characterization of partial chemical reprogramming reveals evidence of cell rejuvenationbioRxiv - Molecular Biology 2023Quote: ... Protein was precipitated from 10 µl of sample by addition of nine volumes of 74.9:24.9:0.2 v/v/v acetonitrile/methanol/formic acid containing internal standards (valine-d8, Sigma-Aldrich; St. Louis, MO; and phenylalanine-d8, Cambridge Isotope Laboratories; Andover, MA). Precipitated material was cleared by centrifugation and the supernatant was injected directly onto a 150 x 2 mm 3 µm Atlantis HILIC column (Waters ...
-
bioRxiv - Neuroscience 2020Quote: ... with a pH of 7.4 containing 6.15 mM NaN3 and 4.64 mM sodium 3-[trimethylsilyl] d4-propionate (Cambridge Isotope Laboratories) was prepared ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were incubated in RPMI containing 2 mM 13C5,15N2-glutamine (Cambridge Isotope Labs) as the sole glutamine source for 2 hours before metabolites were extracted and quantified as described above with the exception that no internal standards were added to the extraction buffer ...
-
bioRxiv - Molecular Biology 2019Quote: ... 0.05 U Phosphodiesterase I (Sigma # P3243)] and [15N]5-2’-deoxyadenosine ([15N]-dA) (Cambridge Isotope Laboratories # NLM-3895-25) as the internal standard to account for variations in sample handling and mass spectrometer response59 ...
-
bioRxiv - Biophysics 2023Quote: ... They were then resuspended in 500 ml of M9 media supplemented with 100 mg of a desired 15N-labeled or 13C-labeled L-amino acid (Cambridge Isotope Labs, Inc. or Sigma-Aldrich, Inc.) as well as the remaining 19 unlabeled amino acids ...
-
bioRxiv - Systems Biology 2021Quote: ... 13C-[6C]-labeled 2-deoxy-D-glucose (Cambridge Isotope Laboratories, Inc., 12 Ci/mmol); [1-3H] 2-Deoxy-D-glucose (ViTrax Radiochemicals) ...
-
bioRxiv - Biochemistry 2019Quote: ... 20 min) and resuspended in M9 minimal media comprising 3 g/L 15N ammonium chloride (Cambridge Isotope Laboratories, NLM-467-25) with 10 g/L glucose or 10 g/L 13C glucose (Cambridge Isotope Laboratories ...
-
bioRxiv - Immunology 2019Quote: Cells were cultured in glucose-free media with 2 mM 13C6-glucose (Cambridge Isotope Laboratories) or [13C5]-ribose-inosine (Omicron Biochemicals ...
-
bioRxiv - Cell Biology 2023Quote: ... Mice were then injected with [U13C] succinate (10 mg/kg, 2 min) (Cambridge Isotope Laboratories) via intravenous tail vein ...
-
bioRxiv - Cell Biology 2022Quote: ... Labelled media refers to EMM with 2% D-Glucose (U-13C6) (Cambridge Isotope Laboratories, Inc.). Once cells came into contact with labelled media ...