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Citations for Cambridge Isotope Labs :
151 - 164 of 164 citations for 6 Cyclohexylcarbamoylcyclohex 3 enecarboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... followed by metabolite extraction in ice cold 80:20 LC/MS grade methanol: water buffer containing 15N and 13C fully-labeled amino acid standards (Cambridge Isotope Laboratories #MSK-A2-1.2). Samples were vortexed for 10 minutes at 4°C and then centrifuged at maximum speed (> 14,000g ...
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Multi-omics characterization of partial chemical reprogramming reveals evidence of cell rejuvenationbioRxiv - Molecular Biology 2023Quote: ... Protein was precipitated from 10 µl of sample by addition of nine volumes of 74.9:24.9:0.2 v/v/v acetonitrile/methanol/formic acid containing internal standards (valine-d8, Sigma-Aldrich; St. Louis, MO; and phenylalanine-d8, Cambridge Isotope Laboratories; Andover, MA). Precipitated material was cleared by centrifugation and the supernatant was injected directly onto a 150 x 2 mm 3 µm Atlantis HILIC column (Waters ...
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bioRxiv - Synthetic Biology 2021Quote: ... a 80:20 mixture of 6-13C glucose:U-13C6 glucose or a 80:20 mixture of 1-13C glucose:U-13C6 glucose (Cambridge Isotope, MA and Omicron Biochemicals, IN). Cultures used as internal standards for metabolomic measurements were grown in two subsequent M9 minimal mediums cultures containing 100% U-13C6.
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bioRxiv - Neuroscience 2020Quote: ... with a pH of 7.4 containing 6.15 mM NaN3 and 4.64 mM sodium 3-[trimethylsilyl] d4-propionate (Cambridge Isotope Laboratories) was prepared ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Samples were thawed on ice and phthalates were extracted from a 10 μL aliquot via vigorous vortexing for 30 min at 4°C in the presence of 5:3:2 methanol:acetonitrile:water (240 μL) containing an isotope labeled standard (1 μM DEHP ring-1,2-13C2, Cambridge Isotope Laboratories). Extraction supernatants were clarified via centrifugation at 18,000 rpm for 10 min at 4°C and analyzed immediately by ultra high-pressure liquid chromatography coupled to mass spectrometry (UHPLC-MS) ...
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bioRxiv - Biophysics 2023Quote: ... They were then resuspended in 500 ml of M9 media supplemented with 100 mg of a desired 15N-labeled or 13C-labeled L-amino acid (Cambridge Isotope Labs, Inc. or Sigma-Aldrich, Inc.) as well as the remaining 19 unlabeled amino acids ...
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bioRxiv - Immunology 2022Quote: ... of the samples was used to scale the metabolite extraction to a ratio of 1×106 cells / 1 mL extraction solution [80% LCMS grade methanol (aq) with 500nM labelled amino acid internal standard (Cambridge Isotope Laboratories, Inc., Cat No. MSK-A2-1.2)] ...
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bioRxiv - Biochemistry 2019Quote: ... 20 min) and resuspended in M9 minimal media comprising 3 g/L 15N ammonium chloride (Cambridge Isotope Laboratories, NLM-467-25) with 10 g/L glucose or 10 g/L 13C glucose (Cambridge Isotope Laboratories ...
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bioRxiv - Molecular Biology 2021Quote: ... expression was carried out in M9 minimal media supplemented with 3 g/L (13C6, 99%)-D-glucose and/or 1 g/l (15N, 99%)-NH4Cl (Cambridge Isotope Laboratories). To generate isotopically labeled rad6-A126T ...
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bioRxiv - Biophysics 2020Quote: ... harvested at an OD600nm of 3 and resuspended in 2 L of M9 minimal medium with isotopic enrichment (1 g/L of 15NH4Cl and/or 3 g/L of 13C6-glucose, Cambridge Isotope Laboratories). After 30-45 min ...
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bioRxiv - Biochemistry 2023Quote: ... 15N-labeled protein samples were grown in 1L minimal media containing 3 g/L 15N ammonium chloride (Cambridge Isotope Laboratories, Tweksbury, MA) and unlabeled protein samples were grown in 1L super broth media ...
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bioRxiv - Developmental Biology 2021Quote: ... Islets were then incubated on a rotator plate at 95 RPM for 90 minutes at 37°C and 5% CO2 before being transferred to Eppendorf tubes and the basal KRB exchanged for a 0.9 ml volume of KRB containing either 3 mM (low) or 17 mM (high) [U-13C6] glucose (Cambridge Isotope Laboratories, CLM 1396). Islets were then incubated for 1 hour at 37°C and 5% CO2 ...
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bioRxiv - Biophysics 2024Quote: ... The cells were used to inoculate 2L of H2O-based M9 media supplemented with 3 g L-1 of [U-13C6] d-glucose (Cambridge Isotope Laboratories, CIL) and 2 g L-1 of [15N] ammonium chloride (Cambridge Isotope Laboratories ...
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bioRxiv - Biochemistry 2022Quote: ... MMA quantification was performed using 3 uL of plasma that was spiked in with [U-13C]-MMA (Cambridge Isotope Laboratories, cat# CLM-9426-PK) as previously described (31 ...