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Citations for Cambridge Isotope Labs :
151 - 200 of 253 citations for 4 Nitrophenyl beta D glucuronic acid sodium salt since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: An internal standard stock mixture was prepared using the following compounds and concentrations: citric acid-d4 (Cambridge Isotope Laboratories, DLM-3487), 225 μM ...
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bioRxiv - Cell Biology 2021Quote: ... and then harvested with 1mL/well of 80% methanol (on dry ice) with 500 nM 13C- and 15N-labeled amino acids (Cat#MSK-A1-1.2, Cambridge Isotope Laboratories) as internal standards ...
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bioRxiv - Neuroscience 2020Quote: ... and SILAC amino acids (13C6-15N2-lysine, 13C6-15N4-arginine (Lys8/Arg10) and D4-lysine/13C6-arginine (Lys4/Arg6)) were obtained from Cambridge Isotope Laboratories ...
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bioRxiv - Cancer Biology 2022Quote: ... and SVVESVKFL+7 were synthesized at the MIT-Koch Institute Swanson Biotechnology Center in Biopolymers and Proteomics Facility using standard Fmoc chemistry using an Intavis model MultiPep peptide synthesizer with HATU activation and 5 μmol chemistry cycles as previously described.(24) Standard Fmoc amino acids were procured from NovaBiochem and Fmoc-Leu (13C6, 15N) was obtained from Cambridge Isotope Laboratories ...
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bioRxiv - Microbiology 2020Quote: ... irregularis was cultured in modified SC solid medium supplied with 1 mM neutralized [1-13C]myristic acid (Cambridge Isotope Laboratories, Inc.) for 2.5 months ...
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bioRxiv - Biochemistry 2021Quote: ... Extraction solution contained 80:20 methanol:water with 1:2500 dilution of a heavy amino acid mix standard (MSK-A2, Cambridge Isotope Laboratories); derivatization solution is described below ...
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bioRxiv - Cell Biology 2021Quote: ... that cannot form lysine-linked polySUMO chains were grown for at least ten divisions in synthetic complete media supplemented either with unlabeled (Lys0 and Arg0; light) or heavy isotope-labeled amino acids (Lys8 and Arg10; heavy) from Cambridge Isotope Laboratories ...
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bioRxiv - Molecular Biology 2022Quote: ... medium (L-arginine:HCL [U13C6] and L-lysine:2HCL [4,4,5,5-D4], or heavy (L-arginine:HCL (13C6, 15N4) and L-lysine:2HCL (13C6, 15N2)) amino acid isotopes (Cambridge Isotope Laboratories Inc.).
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bioRxiv - Cell Biology 2024Quote: ... SRM transitions were detailed in the TraceFinder software and then verified with the Metabolomics Amino Acid Mix Standard (Cambridge Isotope Laboratories) for unique transitions ...
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bioRxiv - Cancer Biology 2023Quote: GMP clones were lysed in 100 µl ice-cold 80% methanol in water and polar metabolites were extracted using methanol-chloroform phase separation (1 ml methanol containing 2.5 µM of an internal standard (13C-, 15N-labeled amino acid mix; Cambridge Isotope Laboratories), 500 µl water ...
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bioRxiv - Cancer Biology 2023Quote: ... Two transitions were optimised using an authentic standard of glycocholic acid (glycine-1-13C, CLM-191-PK, Cambridge Isotope Laobratories, UK), from the negative precursor ion (m/z 465 ...
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bioRxiv - Biochemistry 2023Quote: RPE cells were incubated in pH 7.4 α-MEM and 50 µM [U-13C16]-palmitic acid (Cambridge isotope labs, CLM-409) complexed to bovine serum albumin at a 6:1 ratio of fatty acid to albumin ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were washed three times with 1 ml of cold 0.9% NaCl and scraped into 500µL of cold 80%v/v LC-MS grade methanol containing heavy amino acid internal standards (Cambridge Isotope Laboratories). After 10 minutes of vortexing at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Chromatographic performance was quality controlled using a mixture of unlabeled standard amino acids and a mixture of chemically diverse compounds (Cambridge Isotope Laboratories ...
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bioRxiv - Cell Biology 2023Quote: ... of 80% methanol in LC–MS water containing 500 nM isotope-labelled amino acids used as internal standards (Cambridge Isotope Laboratories). Following extraction ...
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bioRxiv - Systems Biology 2024Quote: ... and either heavy (13C615N4 L-arginine or Arg10 an 13C615N2 L-lysine or Lys8) and light amino acids (Cambridge Isotope Laboratories) at 0.4 mmol/L and 0.8 mmol/L ...
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bioRxiv - Neuroscience 2024Quote: ... with 0.15% formic acid) containing 10 μM of [13C-U] [15N-U] labelled internal standards (MSK-CAA-1; Cambridge Isotope Laboratories). Analytes were separated using a SeQuant ZIC cHILIC 3μm 100Å 150 x 2.1 mm column (EMD Millipore ...
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bioRxiv - Systems Biology 2024Quote: ... 20% LC-MS water) and supplemented with isotopically labelled internal standards (17 amino acids and isotopically labelled reduced glutathione, Cambridge Isotope Laboratories ...
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bioRxiv - Molecular Biology 2022Quote: ... supplemented with 15NH4Cl and when needed 13C-D-glucose as sole nitrogen and carbon sources (purchased from Cambridge Isotope Laboratories). The cells were grown at 37°C before they were induced with 0.2 mM IPTG at an OD600 of 1.2 for TB and 0.8 for M9 minimal medium and incubated at 17°C for 16 h.
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bioRxiv - Molecular Biology 2024Quote: EGM-2 media was replaced with DMEM media containing D-Glucose (U-13C6, CLM-1396-PK, Cambridge Isotope Laboratories Inc.) 3 hours prior to collection ...
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bioRxiv - Molecular Biology 2024Quote: ... 15N labeled protein was expressed in a modified M9 medium containing D-glucose (Cambridge Isotope Laboratories, Inc., U-13C6, 99%) and ammonium chloride (Cambridge Isotope Laboratories ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were washed with 0.9% NaCl twice and lysed with pre-chilled extraction buffer that was composed of 480 ul of MeOH mixed with 120 ul of H2O and 1 uM of pre-mixed Heavy Amino Acid mix (Cambridge Isotope Laboratories). Lysates were transferred to an Eppendorf tube and vortexed at 4 °C for 10 min before being centrifuged at 16,000 g at 4 °C for 10 min ...
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bioRxiv - Cell Biology 2020Quote: ... L amino acids were substituted for the same concentrations of heavy isotope–labeled (H) amino acids 13C6-15N2 L-Lys:2HCl and 13C6-15N4 L-Arg (Cambridge Isotope Laboratories). For immature dendritic cells (Dc ...
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bioRxiv - Cell Biology 2020Quote: Cells were washed twice with ice cold PBS and extracted on dry-ice in 0.8 mL 80% methanol containing 500 nM internal standards (Metabolomics Amino Acid Mix Standard: Cambridge Isotope Laboratories, Inc.). Cell extracts were collected using a cell scraper and transferred to a microcentrifuge tube ...
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bioRxiv - Cell Biology 2022Quote: ... Polar metabolites were extracted in 80 % methanol containing 15N and 13C fully-labeled amino acid standards (MSK-A2-1.2, Cambridge Isotope Laboratories, Inc). Extracts were shaken for 10 min with a vortexer ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% FBS and heavy isotope-labeled amino acids (L-Lysine 13C6, L-Arginine 13C6) (CLM-2247-H-1, CLM-2265-H-1, Cambridge Isotope Laboratories). DAC-treated cells grown in the regular medium were considered as SILAC light counterparts.
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bioRxiv - Cell Biology 2021Quote: ... and 500 μl of ice-cold 80% methanol in water with 250nM 13C/15N labeled amino acid standards (MSK-A2-1.2: Cambridge Isotope Laboratories, Inc.) was added to each well ...
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bioRxiv - Cell Biology 2021Quote: ... which were replaced with either unlabelled amino-acids (Lys0 and Arg0) or stable isotopic forms 13C6 15N2-lysine (Lys8) and 13C6 15N4-arginine (Arg10) (Cambridge Isotope Laboratories). The medium was supplemented with 10% dialyzed FBS using previous published methods (Golebiowski et al ...
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bioRxiv - Microbiology 2021Quote: ... 2 mL of LC-MS grade water containing 0.1 μL of MSK-A2-1.2 stable isotope-labeled amino acid standards (Cambridge Isotope Laboratories, Tewksbury, MA) was added before vortexing for 1 minute ...
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bioRxiv - Biochemistry 2021Quote: ... then 10% of bead volume was lysed with 1% Triton buffer for protein extracts and the remaining 90% was extracted in 80% methanol containing heavy labeled amino acid standards (Cambridge Isotope Labs) on a rotator shaker for 10□min at 4□°C.
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bioRxiv - Cancer Biology 2022Quote: ... H2O/ACN/MeOH supplemented with stable isotopic labeled amino acids solution at 1 nmol/mL (MSK-A2-1.2, Cambridge Isotope Laboratories, USA).
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Sex-specific fear acquisition following early life stress is linked to amygdala glutamate metabolismbioRxiv - Animal Behavior and Cognition 2024Quote: ... U-13C15N-labeled amino acids at a final concentration of 0.25 µM (prepared as 2.5 mM in 0.1 N HCl, Cambridge Isotope Laboratories, MSK-A2-1.2), citric acid d4 at 0.02 µg/mL (dissolved at 100 µg/mL in H2O ...
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bioRxiv - Microbiology 2023Quote: ... or medium (84 mg L−1 13C6 L-arginine and 146 mg L−1 2H4 L-lysine) SILAC amino acids (Cambridge Isotope Laboratories). Medium SILAC labeled HeLa or HeLa S3 cells were used as spike-in control ...
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bioRxiv - Cell Biology 2023Quote: ... and either “Heavy” amino acids (50 μg/ml 13C6, 15N4 Arginine HCl and 100 μg/ml 13C6, 15N2 Lysine 2HCl; Cambridge Isotope Laboratories), or the equivalent “Light” amino acids ...
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bioRxiv - Cancer Biology 2023Quote: ... Media was aspirated from cells and then washed rapidly in ice cold bank saline followed by addition of 500 mL ice-cold 80% methanol in water containing 500 nM each of 17 isotopically labeled 13C/15N amino acids (Cambridge Isotope Laboratories). Alternatively ...
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bioRxiv - Cell Biology 2024Quote: ... samples were extracted using 80% methanol in LC-MS grade water containing 500 nM isotope-labeled amino acids as internal standards (Cambridge Isotope Laboratories). Samples were then vortexed for 10 min at 4 °C ...
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bioRxiv - Genetics 2020Quote: ... injection of 4 mmol/kg of 15N-labeled ammonium chloride (15NH4Cl, Cambridge Isotope Laboratories) 20 min before terminal blood collection via cardiac puncture ...
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bioRxiv - Cancer Biology 2020Quote: ... 10 mM U-13C-glucose or 4 mM U-13C-glutamine (Cambridge Isotope Laboratories), which included control (DMSO ...
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bioRxiv - Molecular Biology 2020Quote: ... cultures were first grown at 37°C in 1 L of LB media until an OD600 of 0.6 before the cells were pelleted and resuspended in 0.5 L M9 minimal media prepared in D2O supplemented with 13C-D-glucose and 15NH4Cl (Cambridge Isotope Laboratories). After 1 hour recovery at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... G7021 and for labeled glucose we used either D-glucose-U-13C (99%, Cambridge Isotope Laboratories, cat no CLM-1396-10) or D-glucose-2-13C glucose (99% ...
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bioRxiv - Biochemistry 2021Quote: ... 450 μL of supernatant were transferred to a new tube and 150 μL of deuterated water were added (D2O, D, 99.9%, Cambridge Isotope Laboratories). Each sample was vortex-mixed for 1 min before transferring into 5 mm SampleJet NMR tubes ...
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bioRxiv - Biophysics 2022Quote: HDX samples of I552A SLO-1 were exchanged as a function of time by a 10-fold dilution into D2O (99% D; Cambridge Isotope) buffer ...
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bioRxiv - Biophysics 2024Quote: ... 4.0 gL-1 of D-glucose provided in specific isotopic ratios (12C, Merek, Germany; 13C, Cambridge Isotope Laboratories, USA; Table S1). 109 cells were harvested at late-exponential growth phase (Figure S3) ...
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bioRxiv - Neuroscience 2023Quote: ... Experiments with deuterated lipids were conducted by replacing glucose with 10mM D-glucose (U-13C6, Cambridge isotope laboratories CLM-1396-PK) for 24h hours prior to treatment with 5 μM Aβ fibrils or no treatment.
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bioRxiv - Microbiology 2023Quote: When the protein samples were prepared for NMR analysis the bacteria were cultured in M9 media containing D-[13C] glucose (U-13C6, 99%, CLM-1396-1, Cambridge Isotope) and 15NH4Cl (15NH4 Chloride ...
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bioRxiv - Cancer Biology 2023Quote: ... no-glucose and no-glutamine media was supplemented with 5 mM of 13C6 D-Glucose (Cambridge Isotope Laboratories, Inc., CLM-1396) and 4 mM unlabeled glutamine ...
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bioRxiv - Cancer Biology 2023Quote: U-[13C]-Glc labelling media consisted of DMEM supplemented with 10 mM D-glucose (U-13C6; CLM-1396; Cambridge Isotope Laboratories), 2 mM glutamine ...
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bioRxiv - Cancer Biology 2020Quote: ... 10μL of media was mixed with 10μL of a mixture of isotopically labeled metabolites and amino acids of known concentrations (Cambridge Isotope Laboratories, Tewksbury, MA) and extracted with 600μL ice-cold HPLC grade methanol ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell pellets were resuspended in 1 mL of 80% methanol solution containing 500 nM internal standards (Metabolomics Amino Acid Mix, Cambridge Isotope Laboratories Inc.). Samples were vortexed at 4 degrees ...
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bioRxiv - Neuroscience 2020Quote: F2 SILAM wide type female mice were purchased from Cambridge Isotope Laboratories and then crossed with regular C57BL6 wild type males by fed with stable isotope labeling using amino acids in cell culture (SILAC) L-lysine (13C6) feed (Cambridge Isotope Laboratories, Inc). After 7 days ...