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Citations for Cambridge Isotope Labs :
101 - 144 of 144 citations for palmitic acid ethoxylated phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2024Quote: ... 20% LC-MS water) and supplemented with isotopically labelled internal standards (17 amino acids and isotopically labelled reduced glutathione, Cambridge Isotope Laboratories ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were washed with 0.9% NaCl twice and lysed with pre-chilled extraction buffer that was composed of 480 ul of MeOH mixed with 120 ul of H2O and 1 uM of pre-mixed Heavy Amino Acid mix (Cambridge Isotope Laboratories). Lysates were transferred to an Eppendorf tube and vortexed at 4 °C for 10 min before being centrifuged at 16,000 g at 4 °C for 10 min ...
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bioRxiv - Cell Biology 2020Quote: ... L amino acids were substituted for the same concentrations of heavy isotope–labeled (H) amino acids 13C6-15N2 L-Lys:2HCl and 13C6-15N4 L-Arg (Cambridge Isotope Laboratories). For immature dendritic cells (Dc ...
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bioRxiv - Cell Biology 2020Quote: Cells were washed twice with ice cold PBS and extracted on dry-ice in 0.8 mL 80% methanol containing 500 nM internal standards (Metabolomics Amino Acid Mix Standard: Cambridge Isotope Laboratories, Inc.). Cell extracts were collected using a cell scraper and transferred to a microcentrifuge tube ...
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bioRxiv - Cell Biology 2022Quote: ... Polar metabolites were extracted in 80 % methanol containing 15N and 13C fully-labeled amino acid standards (MSK-A2-1.2, Cambridge Isotope Laboratories, Inc). Extracts were shaken for 10 min with a vortexer ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% FBS and heavy isotope-labeled amino acids (L-Lysine 13C6, L-Arginine 13C6) (CLM-2247-H-1, CLM-2265-H-1, Cambridge Isotope Laboratories). DAC-treated cells grown in the regular medium were considered as SILAC light counterparts.
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bioRxiv - Cell Biology 2021Quote: ... and 500 μl of ice-cold 80% methanol in water with 250nM 13C/15N labeled amino acid standards (MSK-A2-1.2: Cambridge Isotope Laboratories, Inc.) was added to each well ...
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bioRxiv - Biochemistry 2020Quote: ... and 0.798 mM L-(13C6,15N2)-lysine (Lys“8”)] or “medium” amino acids [0.398 mM L-(15N4)-arginine (Arg“4”) and 0.798 mM L-(D4)-lysine (Lys“4”)] (Cambridge Isotope Laboratories, Tewksbury, USA), and incubated for 2 h ...
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bioRxiv - Cell Biology 2021Quote: ... which were replaced with either unlabelled amino-acids (Lys0 and Arg0) or stable isotopic forms 13C6 15N2-lysine (Lys8) and 13C6 15N4-arginine (Arg10) (Cambridge Isotope Laboratories). The medium was supplemented with 10% dialyzed FBS using previous published methods (Golebiowski et al ...
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bioRxiv - Microbiology 2021Quote: ... 2 mL of LC-MS grade water containing 0.1 μL of MSK-A2-1.2 stable isotope-labeled amino acid standards (Cambridge Isotope Laboratories, Tewksbury, MA) was added before vortexing for 1 minute ...
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bioRxiv - Biochemistry 2022Quote: ... and 0.798 mM L-(13C6,15N2)-lysine (Lys”8”)] or “medium-heavy” amino acids [0.398 mM L-(13C6)-arginine (Arg”6”) and 0.798 mM L-(D4)-lysine (Lys”4”)] (Cambridge Isotope Laboratories, Tewksbury, USA). For chloramphenicol (CAP ...
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bioRxiv - Biochemistry 2021Quote: ... then 10% of bead volume was lysed with 1% Triton buffer for protein extracts and the remaining 90% was extracted in 80% methanol containing heavy labeled amino acid standards (Cambridge Isotope Labs) on a rotator shaker for 10□min at 4□°C.
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bioRxiv - Neuroscience 2023Quote: ... each rat was fed 1g of a pancake that contained 4 µl of [13C]-octanoic acid (Cambridge Isotope Laboratories, Inc, Tewksbury MA). After visually confirming the rats had eaten the entire pancake (in less than 5min or they were omitted from the study) ...
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Sex-specific fear acquisition following early life stress is linked to amygdala glutamate metabolismbioRxiv - Animal Behavior and Cognition 2024Quote: ... U-13C15N-labeled amino acids at a final concentration of 0.25 µM (prepared as 2.5 mM in 0.1 N HCl, Cambridge Isotope Laboratories, MSK-A2-1.2), citric acid d4 at 0.02 µg/mL (dissolved at 100 µg/mL in H2O ...
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bioRxiv - Cell Biology 2023Quote: ... and either “Heavy” amino acids (50 μg/ml 13C6, 15N4 Arginine HCl and 100 μg/ml 13C6, 15N2 Lysine 2HCl; Cambridge Isotope Laboratories), or the equivalent “Light” amino acids ...
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bioRxiv - Cancer Biology 2023Quote: ... Media was aspirated from cells and then washed rapidly in ice cold bank saline followed by addition of 500 mL ice-cold 80% methanol in water containing 500 nM each of 17 isotopically labeled 13C/15N amino acids (Cambridge Isotope Laboratories). Alternatively ...
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bioRxiv - Cancer Biology 2022Quote: ... H2O/ACN/MeOH supplemented with stable isotopic labeled amino acids solution at 1 nmol/mL (MSK-A2-1.2, Cambridge Isotope Laboratories, USA).
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bioRxiv - Microbiology 2023Quote: ... or medium (84 mg L−1 13C6 L-arginine and 146 mg L−1 2H4 L-lysine) SILAC amino acids (Cambridge Isotope Laboratories). Medium SILAC labeled HeLa or HeLa S3 cells were used as spike-in control ...
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bioRxiv - Cell Biology 2024Quote: ... samples were extracted using 80% methanol in LC-MS grade water containing 500 nM isotope-labeled amino acids as internal standards (Cambridge Isotope Laboratories). Samples were then vortexed for 10 min at 4 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... 10μL of media was mixed with 10μL of a mixture of isotopically labeled metabolites and amino acids of known concentrations (Cambridge Isotope Laboratories, Tewksbury, MA) and extracted with 600μL ice-cold HPLC grade methanol ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell pellets were resuspended in 1 mL of 80% methanol solution containing 500 nM internal standards (Metabolomics Amino Acid Mix, Cambridge Isotope Laboratories Inc.). Samples were vortexed at 4 degrees ...
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bioRxiv - Neuroscience 2020Quote: F2 SILAM wide type female mice were purchased from Cambridge Isotope Laboratories and then crossed with regular C57BL6 wild type males by fed with stable isotope labeling using amino acids in cell culture (SILAC) L-lysine (13C6) feed (Cambridge Isotope Laboratories, Inc). After 7 days ...
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bioRxiv - Immunology 2020Quote: ... 25 μl of serum and 0.5 ml of Lipid Extraction Buffer containing 1 μg of D2-oleic acid (Cambridge isotope: DLM-689-0.1) and 1 μg of D14-palmitoleic acid (Cayman ...
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bioRxiv - Microbiology 2023Quote: ... were prepared via protein precipitation with the addition of 9 volumes of 74.9:24.9:0.2 v/v/v acetonitrile/methanol/formic acid containing stable isotope-labeled internal standards (valine-d8, Sigma-Aldrich; and phenylalanine-d8, Cambridge Isotope Laboratories). The samples were centrifuged (10 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... The tissue powder was then weighed into pre-chilled tubes and extracted with methanol (containing 500 nM each of 17 isotopically labeled 13C/15N amino acids (Cambridge Isotope Laboratories, Inc.)) ...
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bioRxiv - Cancer Biology 2022Quote: ... the cell pellet was resuspended into 1 mL of ice-cold 2:2:1 MeOH: ACN: H2O (v/v/v) containing 10 μM stable isotope-labeled canonical amino acid mix (Cambridge Isotope Laboratories, Inc) as internal standards ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 µL of biofluid samples or serial dilutions of a chemical standard library of 149 metabolites were extracted with 45 µL of a 75:25:0.1 HPLC grade acetonitrile:methanol:formic acid extraction mix that included the following labeled stable isotope internal standards:13C-labeled yeast extract (Cambridge Isotope Laboratory, Andover, MA, ISO1), 2H9 choline (Cambridge Isotope Laboratory ...
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bioRxiv - Cancer Biology 2022Quote: ... Dried samples were rehydrated with 2:1 methanol:water into which a mixture of isotopically labeled amino acids of known concentrations (Cambridge Isotope Laboratories, MSK-A2-1.2) was added ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... were diluted using 90 µL of 74.9:24.9:0.2 v/v/v acetonitrile/methanol/formic acid containing stable isotope-labeled internal standards (valine-d8, Isotec; and phenylalanine-d8, Cambridge Isotope Laboratories; Andover, MA). The samples were centrifuged (10 min ...
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bioRxiv - Physiology 2021Quote: ... continuous infusion of stable amino acid isotope [13C6]-phenylalanine (L-phenylalanine, ring-13C6, 99%, CLM-1055-MPT; Cambridge Isotope Laboratories, Inc., Tewksbury, MA, USA) was used for measurement of amino acid kinetics across the limb and incorporation of labeled phenylalanine into muscle as previously described [20] ...
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bioRxiv - Biochemistry 2021Quote: ... Tissue was incubated in Krebs-Ringer buffer (formulations used in each figure specified below below) supplemented with 5 mM glucose and [U-13C]-succinic acid (Cambridge isotope CLM-1571-0.1) or [U-13C]-malate (Cambridge isotope CLM-8065 ...
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bioRxiv - Cancer Biology 2023Quote: 5 μL of adult bovine serum or ftABSB was mixed with 45 μL of HPLC-grade methanol:water (80:20) including 500 nM 13C/15N labeled amino acids (Cambridge Isotope Laboratories, MSK-CAA-1). Samples were vortexed for 15 min at 4°C and insoluble material was sedimented by centrifugation at 16,000 x g for 10 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 μL of media was mixed with 45 μL of HPLC-grade methanol:water (80:20) including 500 nM 13C/15N labeled amino acids (Cambridge Isotope Laboratories, MSK-CAA-1). Samples were then vortexed for 15 min at 4°C ...
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bioRxiv - Biochemistry 2022Quote: Amino acids were quantified from 10 µL plasma precipitated with 180 µl ice-cold methanol containing 1 nmol of stable isotope labelled amino acids (Cambridge Isotope Laboratories, MSK-A2-1.2). Samples were incubated at -20°C for 30 min followed by centrifugation at 4°C (14,000g ...
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bioRxiv - Microbiology 2022Quote: ... were resuspended in 300 μL of LC/MS grade methanol containing a mixture of 17 isotope-labeled amino acids (200 nM, Cambridge Isotope Laboratories, MSK-A2-1.2). To this solution ...
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Multi-omics characterization of partial chemical reprogramming reveals evidence of cell rejuvenationbioRxiv - Molecular Biology 2023Quote: ... Protein was precipitated from 10 µl of sample by addition of nine volumes of 74.9:24.9:0.2 v/v/v acetonitrile/methanol/formic acid containing internal standards (valine-d8, Sigma-Aldrich; St. Louis, MO; and phenylalanine-d8, Cambridge Isotope Laboratories; Andover, MA). Precipitated material was cleared by centrifugation and the supernatant was injected directly onto a 150 x 2 mm 3 µm Atlantis HILIC column (Waters ...
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bioRxiv - Genomics 2023Quote: ... followed by metabolite extraction in ice cold 80:20 LC/MS grade methanol: water buffer containing 15N and 13C fully-labeled amino acid standards (Cambridge Isotope Laboratories #MSK-A2-1.2). Samples were vortexed for 10 minutes at 4°C and then centrifuged at maximum speed (> 14,000g ...
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bioRxiv - Cell Biology 2022Quote: ... Polar metabolites were extracted in ice cold 80% LC/MS grade methanol containing 15N and 13C fully-labeled amino acid standards (Cambridge Isotope Laboratories #MSK-A2-1.2). Tissues were homogenized by Bead Ruptor 24 (Omni International ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed twice with ice-cold 0.9% NaCl and polar metabolites extracted in ice cold 80% LC/MS grade methanol containing 15N and 13C fully-labeled amino acid standards (Cambridge Isotope Laboratories #MSK-A2-1.2). Extracts were vigorously shaken by vortex for 10 minutes at 4°C and spun at 14,000 rpm at 4°C for 10 minutes to remove insoluble cell debris ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 µL of fluid was extracted in 45 µL of 80% methanol containing 500 nM 13C and 15N amino acid standards (Cambridge Isotope Laboratory, MSK-A2-1.2). Samples were vortexed for 15 min ...
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bioRxiv - Biophysics 2023Quote: ... They were then resuspended in 500 ml of M9 media supplemented with 100 mg of a desired 15N-labeled or 13C-labeled L-amino acid (Cambridge Isotope Labs, Inc. or Sigma-Aldrich, Inc.) as well as the remaining 19 unlabeled amino acids ...
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bioRxiv - Cell Biology 2024Quote: ... LysoIP and MockIP samples were resuspended in LC-MS grade 80% methanol (v/v) with isotopically labeled amino acids (Cambridge Isotope Laboratories, Inc. Catalog #MSK-A2-1.2). Metabolites were profiled by liquid chromatography/mass spectrometry (LC/MS ...
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bioRxiv - Systems Biology 2024Quote: ... 10 µL of spent media samples were mixed with 10 µL of known concentration of isotopically labeled internal standards of amino acids (Cambridge Isotope Laboratory, Andover, MA, MSK-A2-1.2). 600 µL of ice cold HPLC grade methanol was added ...
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bioRxiv - Immunology 2022Quote: ... of the samples was used to scale the metabolite extraction to a ratio of 1×106 cells / 1 mL extraction solution [80% LCMS grade methanol (aq) with 500nM labelled amino acid internal standard (Cambridge Isotope Laboratories, Inc., Cat No. MSK-A2-1.2)] ...