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Citations for Cambridge Isotope Labs :
101 - 150 of 197 citations for N Ethyl Benzamid 3 carboxylate 15N 13C2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... we exchanged the centrifuge tube with one containing a 10 mM solution of either 13C1- or 13C2-pyruvate (99 atom % 13C, Cambridge Isotope Laboratories, Andover, MA, USA). We used the mass decline of the centrifuge tube over time to calculate the label uptake ...
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bioRxiv - Molecular Biology 2021Quote: ... and proteins were produced in the M9 media supplemented with 2 g/L [15N]NH4Cl (Cambridge Isotope Laboratories) and 3 g/L d-[13C6]glucose (Cambridge Isotope Laboratories) ...
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bioRxiv - Biochemistry 2021Quote: ... Uniformly 15N-labeled CaM was prepared in M9 minimal medium containing 0.5 g/L 15NH4Cl (Cambridge Isotope Laboratories) as the sole nitrogen source.
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bioRxiv - Microbiology 2021Quote: Growth trials were conducted for each isolate using labeled substrates (> 98% 13C, 99% 15N, Cambridge Isotope Laboratories, Inc.) to track C into 13C labeled microbial biomass as well as respired 13CO2 ...
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bioRxiv - Biochemistry 2024Quote: ... Heavy arginine and lysine contain the isotopes 13C and 15N: L-lysine:2HCl (Cambridge Isotope #CNLM-291-H) and L-arginine:HCl (Cambridge isotope # CNLM-539-H).
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bioRxiv - Systems Biology 2023Quote: ... T87 cells were cultured in 15N-enriched NT-1 liquid medium containing K15NO3 and 15NH415NO3 (Cambridge Isotope Labs), under identical culture conditions ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated with media containing the designated tracer γ-15N-glutamine (Cambridge Isotope Laboratories: NLM-557-1), or U-13C-glucose (Cambridge Isotope Laboratories ...
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bioRxiv - Systems Biology 2024Quote: ... cells were grown at 37°C in minimal medium supplemented with 15N-ammonium chloride (Cambridge Isotope Laboratories, Inc) in the presence of 50 μg/mL of kanamycin to an OD of 0.8 ...
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bioRxiv - Biochemistry 2024Quote: ... cells were grown to OD600 ≈ 0.6 in M9 medium containing 15N-labeled ammonium chloride (Cambridge Isotope Laboratories, Inc.) and induced by adding isopropyl β-d-1-thiogalactopyranoside to a final concentration of 200 µM for 12 h at 30 °C ...
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bioRxiv - Biophysics 2021Quote: ... 13C,15N-labelled 24x’ was expressed using a 1/100 dilution of Bioexpress media (Cambridge Isotope Labs, Tewksbury, MA) in labelled M9 media (51) ...
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bioRxiv - Biophysics 2022Quote: ... Ammonium chloride (15N, 99%), D-glucose (U-13C6, 99%), and deuterium oxide (D, 99.9%) were purchased from Cambridge Isotope Laboratory Inc ...
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bioRxiv - Cancer Biology 2022Quote: ... and re-plated in glutamine free media that was supplemented with either 2 mM 15N-glutamine (Cambridge isotope laboratories) or 2 mM GlutaMAX (represents the 0 hours timepoint of 15N-glutamine tracing) ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were incubated for 24 h in L-L or H-H medium supplemented with either 0.8mM 15N-ammonium chloride (99% enrichment, NLM-467-1) or 0.4mM 15N-leucine (98% enrichment, NLM-142-0.5) purchased from Cambridge Isotope Laboratories ...
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bioRxiv - Plant Biology 2023Quote: ... The plant materials were extracted with the extraction buffer containing 2 μM labeled Met (13C, 15N, Cambridge Isotope Laboratories), 10 μM DL-dithiothreitol (DTT ...
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bioRxiv - Microbiology 2023Quote: ... glycine (2-13C, 99%; 15N, 98%+), L-phenylalanine (D8, 98%), or tyramine:HCl (1,1,2,2-D4, 98%) (Cambridge Isotope Laboratories, Inc.) was added to BHIS containing 0.1% (w/v ...
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bioRxiv - Biophysics 2024Quote: ... 1 L cultures of autoinduction media (Cold Spring Harbor Protocols) were used with 15N labeled ammonium chloride (Cambridge Isotope Laboratories). The final antibiotic concentration was increased to 0.1 mg/mL of kanamycin to reduce plasmid loss and poor yields ...
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bioRxiv - Cancer Biology 2023Quote: ... 50 μL of internal standard solution (U-13C-15N-cysteine, catalog no: CNLM-3871-H-PK, Cambridge Isotope Laboratories catalog) was added ...
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bioRxiv - Microbiology 2024Quote: ... but supplemented with 6.0 g/L of 13C-glucose and 10.0 g/L of 15N-ammonium sulfate (Cambridge Isotope Laboratories) to enable uniform labeling of fungal biomass ...
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bioRxiv - Molecular Biology 2024Quote: ... 15N labeled protein was expressed in a modified M9 medium containing D-glucose (Cambridge Isotope Laboratories, Inc., U-13C6, 99%) and ammonium chloride (Cambridge Isotope Laboratories ...
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bioRxiv - Cell Biology 2021Quote: ... and then harvested with 1mL/well of 80% methanol (on dry ice) with 500 nM 13C- and 15N-labeled amino acids (Cat#MSK-A1-1.2, Cambridge Isotope Laboratories) as internal standards ...
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bioRxiv - Cancer Biology 2022Quote: ... and SVVESVKFL+7 were synthesized at the MIT-Koch Institute Swanson Biotechnology Center in Biopolymers and Proteomics Facility using standard Fmoc chemistry using an Intavis model MultiPep peptide synthesizer with HATU activation and 5 μmol chemistry cycles as previously described.(24) Standard Fmoc amino acids were procured from NovaBiochem and Fmoc-Leu (13C6, 15N) was obtained from Cambridge Isotope Laboratories ...
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bioRxiv - Cancer Biology 2023Quote: GMP clones were lysed in 100 µl ice-cold 80% methanol in water and polar metabolites were extracted using methanol-chloroform phase separation (1 ml methanol containing 2.5 µM of an internal standard (13C-, 15N-labeled amino acid mix; Cambridge Isotope Laboratories), 500 µl water ...
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bioRxiv - Biochemistry 2023Quote: ... The 6% site-specific 13C/15N isotopically labelled oligonucleotide were synthesized on K&A H8 synthesizer using the 2’-deoxyadenosine phosphoramidite (98% 15N) purchased from Cambridge Isotope Laboratories (USA) ...
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bioRxiv - Neuroscience 2024Quote: ... with 0.15% formic acid) containing 10 μM of [13C-U] [15N-U] labelled internal standards (MSK-CAA-1; Cambridge Isotope Laboratories). Analytes were separated using a SeQuant ZIC cHILIC 3μm 100Å 150 x 2.1 mm column (EMD Millipore ...
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bioRxiv - Microbiology 2024Quote: ... three bottles or bags were filled and spiked with [15N]-tracer (99 atm% 15NH4Cl or 98 atm% Na15NO2-, Cambridge Isotope Laboratories) to a final label concentration of 70 to 200 nM ...
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bioRxiv - Cell Biology 2022Quote: ... Polar metabolites were extracted in 80 % methanol containing 15N and 13C fully-labeled amino acid standards (MSK-A2-1.2, Cambridge Isotope Laboratories, Inc). Extracts were shaken for 10 min with a vortexer ...
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bioRxiv - Biochemistry 2021Quote: ... The labeled M9 minimal medium had the same composition except for the replacement of NH4Cl and glucose by [15N]NH4Cl and uniformly labeled [13C]glucose at the same concentrations (99% labeling for both compounds; Cambridge Isotope Laboratories). The growth media were sterilized by filtration (Millex-LG 0.2 µm ...
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bioRxiv - Biophysics 2021Quote: ... Deuterated RNA samples were prepared in the same way using selective deuterated rNTPs (D-H5, H3′, H4′, H5′ and H5′′) and 13C/15N-labeled samples were prepared using labeled rNTPs (Cambridge Isotope Laboratories). The resulting RNAs were purified for NMR studies by gel electrophoresis ...
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bioRxiv - Biophysics 2020Quote: ... The rinsed cells were then used to inoculate 1 L of M9 medium supplemented with 1 g/L 15N-NH4Cl (Cambridge Isotope Labs), 4 g/L dextrose ...
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bioRxiv - Cell Biology 2021Quote: ... and 500 μl of ice-cold 80% methanol in water with 250nM 13C/15N labeled amino acid standards (MSK-A2-1.2: Cambridge Isotope Laboratories, Inc.) was added to each well ...
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bioRxiv - Cancer Biology 2021Quote: ... whereas the medium for the ‘heavy’ labelled cells contained 84 mg/l 15N L-arginine and 175 mg/l C615N2 L-lysine (Cambridge Isotope Laboratories). Cells were regularly tested for mycoplasma and MCF10DCIS.com cells authenticated ...
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bioRxiv - Cancer Biology 2022Quote: ... and re-plated for 12 hours in glutamine free media that was supplemented with either 2 mM 15N-glutamine (Cambridge isotope laboratories) or 2 mM GlutaMAX (Gibco Inc. ...
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bioRxiv - Biophysics 2020Quote: ... Cells were pelleted and transferred to M9 media containing 1 g of 15N-ammonium chloride and 2 g of 13C uniformly label glucose (Cambridge Isotope Laboratories). After transfer ...
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Synthetase and Hydrolase Specificity Collectively Excludes 2’-Deoxyguanosine from Bacterial AlarmonebioRxiv - Microbiology 2024Quote: ... 20 μM FeSO4-EDTA and 0.50 g/L regular NH4Cl (light medium) or 0.51 g/L NH4Cl (15N, 99%, Cambridge Isotope Laboratories, heavy medium) were used ...
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bioRxiv - Cancer Biology 2023Quote: ... Media was aspirated from cells and then washed rapidly in ice cold bank saline followed by addition of 500 mL ice-cold 80% methanol in water containing 500 nM each of 17 isotopically labeled 13C/15N amino acids (Cambridge Isotope Laboratories). Alternatively ...
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bioRxiv - Microbiology 2024Quote: ... 1 g/L of either (NH4)2SO4 (designated as 14N throughout the text) or (15NH4)2SO4 (designated as 15N, Cambridge Isotope Laboratories) was used as a source of nitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... For the 15N isotope labeling cells were cultivated at 37 °C in M9 minimal medium with 1 g/L 15NH4Cl (Cambridge Isotope Laboratories, Inc.).
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bioRxiv - Cancer Biology 2023Quote: ... The tissue powder was then weighed into pre-chilled tubes and extracted with methanol (containing 500 nM each of 17 isotopically labeled 13C/15N amino acids (Cambridge Isotope Laboratories, Inc.)) ...
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bioRxiv - Biophysics 2024Quote: ... The 1 L M9 culture contained 1 g of 15N labelled ammonium chloride and 2 g of 13C uniformly labelled α-D glucose (Cambridge Isotope Laboratories). The M9 culture was incubated for 30 min with shaking at 37 °C and 220 RPM before adding IPTG to 0.5 mM ...
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bioRxiv - Biophysics 2022Quote: ... then centrifuging and resuspending in M9 minimal medium containing 2 g/L unlabeled glucose and 1 g/L 15N-labeled ammonium acetate (Cambridge Isotope Labs, Tewksbury, MA) and induced with 1 mM IPTG ...
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bioRxiv - Biochemistry 2022Quote: ... 1 % P/S and isotope-labelled D3-15N-L-serine (1 mM unless indicated otherwise) and D4-L-alanine 2 mM (Cambridge Isotope Laboratories, MA, USA). Cells were grown further in the labelling media for 16 hours ...
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bioRxiv - Cancer Biology 2023Quote: 5 μL of adult bovine serum or ftABSB was mixed with 45 μL of HPLC-grade methanol:water (80:20) including 500 nM 13C/15N labeled amino acids (Cambridge Isotope Laboratories, MSK-CAA-1). Samples were vortexed for 15 min at 4°C and insoluble material was sedimented by centrifugation at 16,000 x g for 10 min at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... Pellets were reconstituted in 60 μl acetonitrile:ultrapure water (4:1, containing 13C6/15N-isoleucine as internal standard, 40 μM, Cambridge Isotope Laboratories, CNLM-561-H) and vortexed vigorously ...
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bioRxiv - Biophysics 2023Quote: ... Uniformly-labeled 13C 15N Sup35NM samples were prepared by growing BL21(DES)-Rosetta Escherichia coli in the presence of M9 media with 2 g L-1 D-glucose 1H,13C6 and 1 g L-1 of 15N ammonium chloride (Cambridge Isotope Labs, Cambridge, MA). Purified ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 μL of media was mixed with 45 μL of HPLC-grade methanol:water (80:20) including 500 nM 13C/15N labeled amino acids (Cambridge Isotope Laboratories, MSK-CAA-1). Samples were then vortexed for 15 min at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... uniformly 15N-labeled and 13C/15N-labeled Cd-IF1 were over-expressed in M9 media with 15NH4Cl and 15NH4Cl/13C-glucose (Cambridge Isotope Laboratories, Inc. Andover, MA) using the high-yield protein expression protocol [11] and purified as previously described [12] ...
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bioRxiv - Physiology 2023Quote: ... three female FVB mice were fed a spirulina 15N-containg chow for the duration of the study (initial labeling, breeding, pregnancy, and weaning) (Cambridge Isotope Laboratories, Inc., Tewksbury, MA). After 13 weeks of being on a 15N diet ...
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bioRxiv - Genomics 2023Quote: ... followed by metabolite extraction in ice cold 80:20 LC/MS grade methanol: water buffer containing 15N and 13C fully-labeled amino acid standards (Cambridge Isotope Laboratories #MSK-A2-1.2). Samples were vortexed for 10 minutes at 4°C and then centrifuged at maximum speed (> 14,000g ...
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bioRxiv - Cell Biology 2022Quote: ... Polar metabolites were extracted in ice cold 80% LC/MS grade methanol containing 15N and 13C fully-labeled amino acid standards (Cambridge Isotope Laboratories #MSK-A2-1.2). Tissues were homogenized by Bead Ruptor 24 (Omni International ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed twice with ice-cold 0.9% NaCl and polar metabolites extracted in ice cold 80% LC/MS grade methanol containing 15N and 13C fully-labeled amino acid standards (Cambridge Isotope Laboratories #MSK-A2-1.2). Extracts were vigorously shaken by vortex for 10 minutes at 4°C and spun at 14,000 rpm at 4°C for 10 minutes to remove insoluble cell debris ...