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Citations for Cambridge Isotope Labs :
101 - 137 of 137 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... the media was removed and replaced with glutamine-free media supplemented with 10% dFBS and 4 mmol/L U-13C5-L-glutamine (CLM-1822-H, Cambridge Isotope Laboratories) for 2 hours prior to harvest (n = 3 per condition) ...
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bioRxiv - Cell Biology 2023Quote: Mice were injected intraperitoneally with AZD 5 mg/kg, 2 h prior to intravenous tail vein injection with [U13C] succinate (10 mg/kg, 2 min) (Cambridge Isotope Laboratories). Intravenous injections were performed as a bolus over 20 s ...
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bioRxiv - Cancer Biology 2023Quote: ... Plates were then washed with PBS then switched to labeling media containing either 1 mM 13C5-glutamine (CLM-1822-H-PK, Cambridge Isotope Laboratories) or 5 mM 13C1,2-glucose (CLM-504-PK ...
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bioRxiv - Molecular Biology 2022Quote: ... supplemented with 15NH4Cl and when needed 13C-D-glucose as sole nitrogen and carbon sources (purchased from Cambridge Isotope Laboratories). The cells were grown at 37°C before they were induced with 0.2 mM IPTG at an OD600 of 1.2 for TB and 0.8 for M9 minimal medium and incubated at 17°C for 16 h.
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bioRxiv - Molecular Biology 2024Quote: EGM-2 media was replaced with DMEM media containing D-Glucose (U-13C6, CLM-1396-PK, Cambridge Isotope Laboratories Inc.) 3 hours prior to collection ...
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bioRxiv - Molecular Biology 2024Quote: ... 15N labeled protein was expressed in a modified M9 medium containing D-glucose (Cambridge Isotope Laboratories, Inc., U-13C6, 99%) and ammonium chloride (Cambridge Isotope Laboratories ...
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bioRxiv - Biochemistry 2022Quote: ... Cell pellets were resuspended in 250 mL of isotopically labelled minimal media M9 salt solution supplemented with 13C D-glucose 4.0 g/L and 15NH4Cl 1.0 g/L (Cambridge Isotope Laboratories, Inc.), then incubated to allow the recovery of growth and clearance of unlabeled metabolites ...
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bioRxiv - Molecular Biology 2020Quote: ... cultures were first grown at 37°C in 1 L of LB media until an OD600 of 0.6 before the cells were pelleted and resuspended in 0.5 L M9 minimal media prepared in D2O supplemented with 13C-D-glucose and 15NH4Cl (Cambridge Isotope Laboratories). After 1 hour recovery at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... G7021 and for labeled glucose we used either D-glucose-U-13C (99%, Cambridge Isotope Laboratories, cat no CLM-1396-10) or D-glucose-2-13C glucose (99% ...
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bioRxiv - Biochemistry 2021Quote: ... 450 μL of supernatant were transferred to a new tube and 150 μL of deuterated water were added (D2O, D, 99.9%, Cambridge Isotope Laboratories). Each sample was vortex-mixed for 1 min before transferring into 5 mm SampleJet NMR tubes ...
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bioRxiv - Biophysics 2022Quote: HDX samples of I552A SLO-1 were exchanged as a function of time by a 10-fold dilution into D2O (99% D; Cambridge Isotope) buffer ...
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bioRxiv - Biophysics 2024Quote: ... 4.0 gL-1 of D-glucose provided in specific isotopic ratios (12C, Merek, Germany; 13C, Cambridge Isotope Laboratories, USA; Table S1). 109 cells were harvested at late-exponential growth phase (Figure S3) ...
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bioRxiv - Neuroscience 2023Quote: ... Experiments with deuterated lipids were conducted by replacing glucose with 10mM D-glucose (U-13C6, Cambridge isotope laboratories CLM-1396-PK) for 24h hours prior to treatment with 5 μM Aβ fibrils or no treatment.
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bioRxiv - Microbiology 2023Quote: When the protein samples were prepared for NMR analysis the bacteria were cultured in M9 media containing D-[13C] glucose (U-13C6, 99%, CLM-1396-1, Cambridge Isotope) and 15NH4Cl (15NH4 Chloride ...
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bioRxiv - Cancer Biology 2023Quote: ... no-glucose and no-glutamine media was supplemented with 5 mM of 13C6 D-Glucose (Cambridge Isotope Laboratories, Inc., CLM-1396) and 4 mM unlabeled glutamine ...
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bioRxiv - Cancer Biology 2023Quote: U-[13C]-Glc labelling media consisted of DMEM supplemented with 10 mM D-glucose (U-13C6; CLM-1396; Cambridge Isotope Laboratories), 2 mM glutamine ...
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bioRxiv - Microbiology 2024Quote: ... Pellets were reconstituted in 60 μl acetonitrile:ultrapure water (4:1, containing 13C6/15N-isoleucine as internal standard, 40 μM, Cambridge Isotope Laboratories, CNLM-561-H) and vortexed vigorously ...
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bioRxiv - Molecular Biology 2021Quote: ... expression was carried out in M9 minimal media supplemented with 3 g/L (13C6, 99%)-D-glucose and/or 1 g/l (15N, 99%)-NH4Cl (Cambridge Isotope Laboratories). To generate isotopically labeled rad6-A126T ...
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bioRxiv - Biophysics 2021Quote: ... Deuterated RNA samples were prepared in the same way using selective deuterated rNTPs (D-H5, H3′, H4′, H5′ and H5′′) and 13C/15N-labeled samples were prepared using labeled rNTPs (Cambridge Isotope Laboratories). The resulting RNAs were purified for NMR studies by gel electrophoresis ...
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bioRxiv - Pathology 2024Quote: ... and control uninfected mice were injected intraperitoneally with either 1 g/kg of 13C6-labeled D-glucose (CLM-1396-1, Cambridge Isotope Laboratories) dissolved in sterile PBS or 0.5 mg/gram of body weight 13C3 Sodium L-lactate (CLM-1579-0.5 ...
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bioRxiv - Biophysics 2024Quote: ... M9 minimal media containing 1 g/L of 15NH4Cl and 3 g/L of [13C-6]-D-glucose (or natural abundance glucose) (Cambridge Isotope Laboratories) were used ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 µL of urine was mixed with 90 µL of methanol: water (1:1) and internal standards (Labeled tryptophan (CNLM-2475-H-PK, Cambridge Isotope Laboratories, Inc., Andover, MA), zeatine and inosine ...
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bioRxiv - Microbiology 2021Quote: ... uniformly 15 N- and 13 C-labeled proteins were produced by growing cell cultures in M9 minimal medium that contained 1 g/l 15 N-ammonium chloride and 2 g/l 13 C-D-glucose (Cambridge Isotope Laboratories, Inc.) as the sources of nitrogen and carbon ...
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bioRxiv - Biochemistry 2021Quote: ... 13C-1 labelled polyM was produced from fermentation of the same strain using 13C-1 (99 % 13C) D-fructose (Cambridge Isotope Laboratories, USA). This substrate was further subjected to acid hydrolysis as described elsewhere (39 ...
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bioRxiv - Molecular Biology 2020Quote: ... Unlabeled samples were prepared using Luria Broth (LB) (Melford) and minimal media M9 with 15NH4Cl and/or D- [13C] glucose (Cambridge Isotope Laboratories, Inc) were used to prepare the labeled samples (25) ...
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bioRxiv - Biophysics 2024Quote: ... The cells were used to inoculate 2L of H2O-based M9 media supplemented with 3 g L-1 of [U-13C6] d-glucose (Cambridge Isotope Laboratories, CIL) and 2 g L-1 of [15N] ammonium chloride (Cambridge Isotope Laboratories ...
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bioRxiv - Biophysics 2024Quote: ... The 1 L M9 culture contained 1 g of 15N labelled ammonium chloride and 2 g of 13C uniformly labelled α-D glucose (Cambridge Isotope Laboratories). The M9 culture was incubated for 30 min with shaking at 37 °C and 220 RPM before adding IPTG to 0.5 mM ...
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bioRxiv - Cell Biology 2024Quote: ... grown in monolayers were switched to glucose free media for 3 hours and incubated in 5mm D-glucose (unlabeled) or 13C6- glucose (Cambridge Isotope Laboratories, Inc) for 6 hours ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were prepared using 500uL of sample mixed with 500uL of an NMR buffer containing a chemical shift reference and analytical standard (TSP-2, 2, 3, 3-d4 (D,98% Sodium-3-Trimethylsilyl-Propionate, Cambridge Isotope Laboratories, Inc.). The NMR buffer was prepared with Mono- and Dibasic Potassium Phosphate at 0.5M concentration and a pH of 7 ...
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bioRxiv - Cancer Biology 2022Quote: 500k BMDMs were seeded on 50 mm2 varied stiffness ECM coated PA-gels and cultured for 24h in 25 mM glucose DMEM or 20-22 h in 25 mM glucose DMEM followed by medium change to DMEM lacking glutamine (Gibco, 11960044 replaced with with 13C5-glutamine (Cambridge Isotope Laboratories, CLM-1822-H-PK) for 2 h ...
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bioRxiv - Neuroscience 2023Quote: ... neurons were gently washed with PBS twice and switched into media containing the same components except for replacing light lysine with heavy stable isotope labeled (13C615N2) lysine (Cambridge Isotope Laboratories #CNLM-291-H-PK). For multiple time point experiments ...
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bioRxiv - Cancer Biology 2023Quote: ... Tracing of glutamine was performed by supplementing no-glucose and no-glutamine media with 4 mM 13C5 L-glutamine (Cambridge Isotope Laboratories, Inc., CLM-1822-H) and 5 mM unlabeled glucose ...
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bioRxiv - Developmental Biology 2022Quote: ... and L-Lysine (0.2 mM) were replaced with the non-radioactive isotopic forms Arg-10 (13C6 H14 15N4O2; Cambridge Isotope Laboratories, cat. no. CNLM-539-H-PK) and Lys-8 (13C6 H14 15N2O2 ...
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bioRxiv - Biophysics 2023Quote: ... The exo-PS accumulated on the 10 kDa membrane surface as a clear gel and was collected and dialyzed extensively against MilliQ-grade H2O or D2O (Cambridge Isotope Labs, 99.9% D). The H2O solutions provide scattering profiles at an additional contrast ...
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bioRxiv - Biophysics 2023Quote: ... Uniformly-labeled 13C 15N Sup35NM samples were prepared by growing BL21(DES)-Rosetta Escherichia coli in the presence of M9 media with 2 g L-1 D-glucose 1H,13C6 and 1 g L-1 of 15N ammonium chloride (Cambridge Isotope Labs, Cambridge, MA). Purified ...
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Metabolic function in aging retina and retinal pigment epithelium remains robust despite vision lossbioRxiv - Biochemistry 2021Quote: Either 5 mM U-13C glucose alone or 5 mM unlabeled glucose and 2 mM U-13C glutamine U-13C metabolic tracers (99% isotopic purity) included 13C labeled D-Glucose and L-Glutamine (Cambridge Isotope Laboratories, Inc; Tewksbury, MA). Incubations ...
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bioRxiv - Microbiology 2022Quote: ... DNA with different levels of 13C enrichment was produced by varying the ratio of unlabeled glucose to uniformly-labeled 13C6-D-glucose (Cambridge Isotope Laboratories; CLM-1396; 99 atom %), e.g ...