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Citations for Cambridge Isotope Labs :
101 - 150 of 249 citations for 7 Methoxy 9 methylphenazine 1 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: RPE cells were incubated in pH 7.4 α-MEM and 50 µM [U-13C16]-palmitic acid (Cambridge isotope labs, CLM-409) complexed to bovine serum albumin at a 6:1 ratio of fatty acid to albumin ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were washed three times with 1 ml of cold 0.9% NaCl and scraped into 500µL of cold 80%v/v LC-MS grade methanol containing heavy amino acid internal standards (Cambridge Isotope Laboratories). After 10 minutes of vortexing at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Chromatographic performance was quality controlled using a mixture of unlabeled standard amino acids and a mixture of chemically diverse compounds (Cambridge Isotope Laboratories ...
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bioRxiv - Cell Biology 2023Quote: ... of 80% methanol in LC–MS water containing 500 nM isotope-labelled amino acids used as internal standards (Cambridge Isotope Laboratories). Following extraction ...
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bioRxiv - Cell Biology 2024Quote: ... SRM transitions were detailed in the TraceFinder software and then verified with the Metabolomics Amino Acid Mix Standard (Cambridge Isotope Laboratories) for unique transitions ...
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bioRxiv - Cell Biology 2019Quote: 3 μL of human plasma were spiked with 3 μL amino acid isotope labelled internal standards (Cambridge Isotope Laboratories, #MSK-A2-1.2) and extracted with 250 μL –20 °C methanol for 10 min and centrifuged at 4°C for 10 min at 15 000 g ...
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bioRxiv - Cell Biology 2020Quote: ... L amino acids were substituted for the same concentrations of heavy isotope–labeled (H) amino acids 13C6-15N2 L-Lys:2HCl and 13C6-15N4 L-Arg (Cambridge Isotope Laboratories). For immature dendritic cells (Dc ...
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bioRxiv - Cell Biology 2020Quote: Cells were washed twice with ice cold PBS and extracted on dry-ice in 0.8 mL 80% methanol containing 500 nM internal standards (Metabolomics Amino Acid Mix Standard: Cambridge Isotope Laboratories, Inc.). Cell extracts were collected using a cell scraper and transferred to a microcentrifuge tube ...
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bioRxiv - Cell Biology 2022Quote: ... Polar metabolites were extracted in 80 % methanol containing 15N and 13C fully-labeled amino acid standards (MSK-A2-1.2, Cambridge Isotope Laboratories, Inc). Extracts were shaken for 10 min with a vortexer ...
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bioRxiv - Cell Biology 2021Quote: ... and 500 μl of ice-cold 80% methanol in water with 250nM 13C/15N labeled amino acid standards (MSK-A2-1.2: Cambridge Isotope Laboratories, Inc.) was added to each well ...
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bioRxiv - Biochemistry 2020Quote: ... and 0.798 mM L-(13C6,15N2)-lysine (Lys“8”)] or “medium” amino acids [0.398 mM L-(15N4)-arginine (Arg“4”) and 0.798 mM L-(D4)-lysine (Lys“4”)] (Cambridge Isotope Laboratories, Tewksbury, USA), and incubated for 2 h ...
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bioRxiv - Cell Biology 2021Quote: ... which were replaced with either unlabelled amino-acids (Lys0 and Arg0) or stable isotopic forms 13C6 15N2-lysine (Lys8) and 13C6 15N4-arginine (Arg10) (Cambridge Isotope Laboratories). The medium was supplemented with 10% dialyzed FBS using previous published methods (Golebiowski et al ...
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bioRxiv - Microbiology 2021Quote: ... 2 mL of LC-MS grade water containing 0.1 μL of MSK-A2-1.2 stable isotope-labeled amino acid standards (Cambridge Isotope Laboratories, Tewksbury, MA) was added before vortexing for 1 minute ...
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bioRxiv - Biochemistry 2022Quote: ... and 0.798 mM L-(13C6,15N2)-lysine (Lys”8”)] or “medium-heavy” amino acids [0.398 mM L-(13C6)-arginine (Arg”6”) and 0.798 mM L-(D4)-lysine (Lys”4”)] (Cambridge Isotope Laboratories, Tewksbury, USA). For chloramphenicol (CAP ...
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bioRxiv - Biochemistry 2021Quote: ... then 10% of bead volume was lysed with 1% Triton buffer for protein extracts and the remaining 90% was extracted in 80% methanol containing heavy labeled amino acid standards (Cambridge Isotope Labs) on a rotator shaker for 10□min at 4□°C.
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bioRxiv - Neuroscience 2023Quote: ... each rat was fed 1g of a pancake that contained 4 µl of [13C]-octanoic acid (Cambridge Isotope Laboratories, Inc, Tewksbury MA). After visually confirming the rats had eaten the entire pancake (in less than 5min or they were omitted from the study) ...
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bioRxiv - Cell Biology 2023Quote: ... and either “Heavy” amino acids (50 μg/ml 13C6, 15N4 Arginine HCl and 100 μg/ml 13C6, 15N2 Lysine 2HCl; Cambridge Isotope Laboratories), or the equivalent “Light” amino acids ...
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bioRxiv - Cancer Biology 2023Quote: ... Media was aspirated from cells and then washed rapidly in ice cold bank saline followed by addition of 500 mL ice-cold 80% methanol in water containing 500 nM each of 17 isotopically labeled 13C/15N amino acids (Cambridge Isotope Laboratories). Alternatively ...
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Sex-specific fear acquisition following early life stress is linked to amygdala glutamate metabolismbioRxiv - Animal Behavior and Cognition 2024Quote: ... U-13C15N-labeled amino acids at a final concentration of 0.25 µM (prepared as 2.5 mM in 0.1 N HCl, Cambridge Isotope Laboratories, MSK-A2-1.2), citric acid d4 at 0.02 µg/mL (dissolved at 100 µg/mL in H2O ...
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bioRxiv - Cancer Biology 2020Quote: ... 10μL of media was mixed with 10μL of a mixture of isotopically labeled metabolites and amino acids of known concentrations (Cambridge Isotope Laboratories, Tewksbury, MA) and extracted with 600μL ice-cold HPLC grade methanol ...
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bioRxiv - Neuroscience 2020Quote: F2 SILAM wide type female mice were purchased from Cambridge Isotope Laboratories and then crossed with regular C57BL6 wild type males by fed with stable isotope labeling using amino acids in cell culture (SILAC) L-lysine (13C6) feed (Cambridge Isotope Laboratories, Inc). After 7 days ...
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bioRxiv - Microbiology 2023Quote: ... were prepared via protein precipitation with the addition of 9 volumes of 74.9:24.9:0.2 v/v/v acetonitrile/methanol/formic acid containing stable isotope-labeled internal standards (valine-d8, Sigma-Aldrich; and phenylalanine-d8, Cambridge Isotope Laboratories). The samples were centrifuged (10 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... The tissue powder was then weighed into pre-chilled tubes and extracted with methanol (containing 500 nM each of 17 isotopically labeled 13C/15N amino acids (Cambridge Isotope Laboratories, Inc.)) ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... were diluted using 90 µL of 74.9:24.9:0.2 v/v/v acetonitrile/methanol/formic acid containing stable isotope-labeled internal standards (valine-d8, Isotec; and phenylalanine-d8, Cambridge Isotope Laboratories; Andover, MA). The samples were centrifuged (10 min ...
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bioRxiv - Physiology 2021Quote: ... continuous infusion of stable amino acid isotope [13C6]-phenylalanine (L-phenylalanine, ring-13C6, 99%, CLM-1055-MPT; Cambridge Isotope Laboratories, Inc., Tewksbury, MA, USA) was used for measurement of amino acid kinetics across the limb and incorporation of labeled phenylalanine into muscle as previously described [20] ...
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bioRxiv - Biochemistry 2021Quote: ... Tissue was incubated in Krebs-Ringer buffer (formulations used in each figure specified below below) supplemented with 5 mM glucose and [U-13C]-succinic acid (Cambridge isotope CLM-1571-0.1) or [U-13C]-malate (Cambridge isotope CLM-8065 ...
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bioRxiv - Microbiology 2022Quote: ... were resuspended in 300 μL of LC/MS grade methanol containing a mixture of 17 isotope-labeled amino acids (200 nM, Cambridge Isotope Laboratories, MSK-A2-1.2). To this solution ...
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bioRxiv - Cell Biology 2022Quote: ... Polar metabolites were extracted in ice cold 80% LC/MS grade methanol containing 15N and 13C fully-labeled amino acid standards (Cambridge Isotope Laboratories #MSK-A2-1.2). Tissues were homogenized by Bead Ruptor 24 (Omni International ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed twice with ice-cold 0.9% NaCl and polar metabolites extracted in ice cold 80% LC/MS grade methanol containing 15N and 13C fully-labeled amino acid standards (Cambridge Isotope Laboratories #MSK-A2-1.2). Extracts were vigorously shaken by vortex for 10 minutes at 4°C and spun at 14,000 rpm at 4°C for 10 minutes to remove insoluble cell debris ...
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bioRxiv - Genomics 2023Quote: ... followed by metabolite extraction in ice cold 80:20 LC/MS grade methanol: water buffer containing 15N and 13C fully-labeled amino acid standards (Cambridge Isotope Laboratories #MSK-A2-1.2). Samples were vortexed for 10 minutes at 4°C and then centrifuged at maximum speed (> 14,000g ...
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Multi-omics characterization of partial chemical reprogramming reveals evidence of cell rejuvenationbioRxiv - Molecular Biology 2023Quote: ... Protein was precipitated from 10 µl of sample by addition of nine volumes of 74.9:24.9:0.2 v/v/v acetonitrile/methanol/formic acid containing internal standards (valine-d8, Sigma-Aldrich; St. Louis, MO; and phenylalanine-d8, Cambridge Isotope Laboratories; Andover, MA). Precipitated material was cleared by centrifugation and the supernatant was injected directly onto a 150 x 2 mm 3 µm Atlantis HILIC column (Waters ...
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bioRxiv - Biophysics 2023Quote: ... They were then resuspended in 500 ml of M9 media supplemented with 100 mg of a desired 15N-labeled or 13C-labeled L-amino acid (Cambridge Isotope Labs, Inc. or Sigma-Aldrich, Inc.) as well as the remaining 19 unlabeled amino acids ...
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bioRxiv - Plant Biology 2023Quote: ... and 1 g/L KNO3 or 1 g/L K15NO3 (Cambridge Isotope Laboratories), pH 5.8 ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1 g/L KNO3 or 1 g/L K15NO3 (Cambridge Isotope Laboratories), pH 5.8 ...
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bioRxiv - Biophysics 2023Quote: ... Uniformly-labeled 13C 15N Sup35NM samples were prepared by growing BL21(DES)-Rosetta Escherichia coli in the presence of M9 media with 2 g L-1 D-glucose 1H,13C6 and 1 g L-1 of 15N ammonium chloride (Cambridge Isotope Labs, Cambridge, MA). Purified ...
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bioRxiv - Physiology 2020Quote: ... constant infusion (0.0085 mg•kg-1•min-1) of [ring-13C6]-L-phenylalanine (Cambridge Isotope Laboratories, Tewksbury, MA) from 1000 to the end of the protocol ...
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bioRxiv - Biochemistry 2019Quote: ... with 1 g 15NH4Cl (Cambridge Isotope Laboratories) as the sole nitrogen source ...
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bioRxiv - Bioengineering 2022Quote: ... 100 % 1-13C glucose (Cambridge Isotope Laboratories), or unlabelled glucose ...
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bioRxiv - Biochemistry 2022Quote: ... 1 g/L 15NH4Cl (Cambridge Isotope Laboratories) and 50 μg/mL kanamycin ...
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bioRxiv - Cancer Biology 2020Quote: ... Heavy lysine (1G: CLM-265-H-1) and arginine (1G: CNLM-291-H-1) were purchased from Cambridge Isotope Laboratories (CIL ...
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bioRxiv - Cell Biology 2022Quote: ... TKO and Glut 5 were gavaged with a mix of 1:1 universally labeled fructose (U-13C6, Cambridge Isotope Laboratories) and regular glucose at 1g/kg body weight ...
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bioRxiv - Biochemistry 2019Quote: ... 13C6-glucose (Cambridge Isotope Laboratories CLM-1396-1), Vitamin B5-[13C6 15N2] (calcium pantothenate-[13C6 15N2] ...
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bioRxiv - Cancer Biology 2020Quote: ... 99% (Cambridge Isotope Laboratories, Inc., CLM-1396-1) and 2 mL of bovine pituitary extract ...
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bioRxiv - Biophysics 2021Quote: ... [1-13C]pyruvate (Cambridge Isotope Labs, Massachusetts, USA) with 15mM Ox063 (Oxford Instruments ...
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bioRxiv - Cancer Biology 2021Quote: ... PaTu-8902 and MIAPaCa-2 iDox-shGOT2.1 cells were cultured in media containing 1 mM unlabelled glucose and 1 mM U13C-Pyruvate (Cambridge Isotope Laboratories) for 16 hours.
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bioRxiv - Cancer Biology 2020Quote: ... TIF was mixed 1:1 with a solution of isotopically labeled glucose at a known concentration (Cambridge Isotope Laboratories, CLM-1396). Samples were extracted in 300 µl of ice cold HPLC grade methanol ...
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bioRxiv - Biochemistry 2019Quote: ... assay and immediately diluted (1:1) to a final protein concentration of 0.5 mg/mL with either 18O heavy (Cambridge Isotope Laboratories) or 16O light (Fisher ...
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bioRxiv - Plant Biology 2019Quote: ... or 15N MS medium (1/2 MS without nitrogen source [PhytoTechnology Laboratories], 15NH415NO3 [0.5 g/L, NLM-390-1, Cambridge Isotope Laboratory] ...
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bioRxiv - Biophysics 2020Quote: ... The rinsed cells were then used to inoculate 1 L of M9 medium supplemented with 1 g/L 15N-NH4Cl (Cambridge Isotope Labs), 4 g/L dextrose ...
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bioRxiv - Biochemistry 2023Quote: ... To the dried peptide, 300 μL of a 1:1 mixture 12 M DCl (35% w/v, Aldrich) and D2O (Cambridge Isotope Laboratory) containing 3% phenol (w/v ...