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Citations for Cambridge Isotope Labs :
51 - 100 of 135 citations for Hexadecanoic acid reaction products with tetraethylenepentamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and isotopically labeled 17 amino acids (at 1/5000, Cambridge Isotope Laboratories, MSK-A2-1.2) and isotopically labeled reduced glutathione (at 1 μM ...
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bioRxiv - Biochemistry 2024Quote: ... We obtained 13C6-nicotinic acid (CLM-9954) and D4-nicotinamide (DLM-6883) from Cambridge Isotope Laboratories (Cambridge ...
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bioRxiv - Biochemistry 2021Quote: ... with inclusion of 3 µL 100 µM labeled (13C,15N) amino acid standard mix (Cambridge Isotope Laboratories ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were processed as mentioned above except that [1,2,3,4-13C4]L-malic acid (Cambridge Isotope Laboratories) was added as an internal standard and dried extracts were derivatized with diacetyl-L-tartaric anhydride (DATAN ...
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bioRxiv - Molecular Biology 2022Quote: ... and 60 mg/L of alpha-ketoisovaleric acid (13C5, 98%; 3-D1, 98%, Cambridge Isotope Laboratory). All the recombinant protein expressions were performed at 37°C during 4 hours in presence of 1 mM IPTG.
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bioRxiv - Molecular Biology 2022Quote: ... 100 mg/L of alpha-ketobutyric acid (methyl-13C, 99%; 3,3-D2, 98%, Cambridge Isotope Laboratory) and 60 mg/L of alpha-ketoisovaleric acid (13C5 ...
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bioRxiv - Cell Biology 2022Quote: ... This was supplemented with 500 nM isotopically labelled amino acids (Cambridge Isotope Laboratories. Cat# MSK-A2-S).
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bioRxiv - Genomics 2024Quote: ... and heavy amino acid isotopes (L-lysine, +8Da; Cambridge Isotope Laboratories, #CNLM291H; L-arginine +10Da; Cambridge Isotope Laboratories ...
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bioRxiv - Plant Biology 2024Quote: ... v/v) and 5% 1 M hydrochloric acid] with a [ring-13C6]-labeled dopamine (Cambridge Isotope Laboratories) at concentrations of 5 mM ...
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bioRxiv - Cell Biology 2020Quote: ... and extracted with 80% methanol containing 500 nM isotope-labeled amino acids as internal standards (Cambridge Isotope Laboratories). Where indicated in the section “Mitochondrial isolations for metabolite analyses” a different extraction method was used to specifically quantify NAD levels ...
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bioRxiv - Cell Biology 2021Quote: ... the reactions were supplemented with either normal isotopic ATP ([16O]ATP) or heavy ATP ([18O]ATP) (Cambridge Isotope Laboratories). To compare Ndd1 phosphorylation at low initial substrate turnover and high turnover ...
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bioRxiv - Cell Biology 2020Quote: ... L amino acids were substituted for the same concentrations of intermediate isotope–labeled (M) amino acids 4.4.5.5-D4 L-Lys:2HCl and 13C6 L-Arg (Cambridge Isotope Laboratories). All media were changed every other day for 8 to 10 days ...
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bioRxiv - Microbiology 2021Quote: ... Commercial reagents and standards for analysis were purchased from the following suppliers: Deuterated Dimethylsulfoxide (DMSO-d6, 99.9%) and 4,4-dimethyl-4-silapentane-1-sulfonic acid (Cambridge Isotope Laboratories ...
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bioRxiv - Cancer Biology 2020Quote: ... metabolites were extracted with 80:20 methanol:water (−80°C) containing stable isotope-labeled internal standard [1,2,3,4-13C4]α-ketoglutaric acid (Cambridge Isotope Laboratories) and incubated at −80°C for 1 hr as described previously63,64 ...
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bioRxiv - Cell Biology 2022Quote: ... 600 μl LC/MS grade methanol containing 500 nM internal standards (Amino Acid Metabolomics Mix; Cambridge Isotope Laboratories, Inc.) was added to each well ...
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bioRxiv - Cell Biology 2023Quote: ... Lipids were extracted according to a modified Folch method62 using 50 μM tridecanoic-d25 acid (Cambridge Isotope Laboratories, Inc.) as an internal standard ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... NY) and 0.26 mM internal standard (4,4-dimethyl-4-silapentane-1-sulfonic acid/DSS, Cambridge Isotope Laboratory, Andover, MA). Briefly ...
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bioRxiv - Biochemistry 2021Quote: ... 25.9 mM NaHCO3) supplemented with 5 mM glucose and 50 μM [U-13C]-succinic acid (Cambridge isotope CLM-1571-0.1). This buffer was pre-equilibrated at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... and 0.798 mM L-(13C6,15N2)-lysine (Lys”8”)] or “medium” amino acids [0.398 mM L-(15N4)-arginine (Arg”4”) and 0.798 mM L-(D4)-lysine (Lys”4”)] (Cambridge Isotope Laboratories), and incubated for 2 hr as previously described elsewhere (Imami et al. ...
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bioRxiv - Genetics 2024Quote: ... Polar metabolites were extracted in 500μL ice-cold 80% methanol containing amino acid standards (Cambridge Isotope Labs, MSK-A2-1.2). Samples were vortexed at 4°C for 10 minutes and centrifuged for 10 minutes at 20000xg at 4°C before supernatant was collected and dried via nitrogen evaporation ...
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bioRxiv - Cancer Biology 2023Quote: ... Metabolites were extracted in 1 mL ice-cold 80% methanol containing 500 nM internal amino acid standards (Cambridge Isotope Laboratories). Following a 10 min vortex and centrifugation at 21130 g for 3 min at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... L-Tryptophan (indole-d5, 98%) and indoleacetic acid (2,2-d2, 96%)) of the highest purity grade available were obtained from Cambridge Isotope Laboratories Inc.
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bioRxiv - Cancer Biology 2023Quote: ... and resuspended in different volumes of water containing labeled non-standard amino acid mix (Cambridge Isotope Laboratories, MSK-NCAA-1) to account for differences in starting tissue weight.
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bioRxiv - Physiology 2022Quote: ... ice-cold 400μL saline and ice-cold dH2O containing amino acid isotope labelled internal standards (Cambridge Isotope Laboratories, #MSK-A2-1.2). An aliquot of homogenate (50μL ...
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bioRxiv - Biochemistry 2020Quote: An internal standard stock mixture was prepared using the following compounds and concentrations: citric acid-d4 (Cambridge Isotope Laboratories, DLM-3487), 225 μM ...
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bioRxiv - Cell Biology 2021Quote: ... and then harvested with 1mL/well of 80% methanol (on dry ice) with 500 nM 13C- and 15N-labeled amino acids (Cat#MSK-A1-1.2, Cambridge Isotope Laboratories) as internal standards ...
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bioRxiv - Neuroscience 2020Quote: ... and SILAC amino acids (13C6-15N2-lysine, 13C6-15N4-arginine (Lys8/Arg10) and D4-lysine/13C6-arginine (Lys4/Arg6)) were obtained from Cambridge Isotope Laboratories ...
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bioRxiv - Cancer Biology 2022Quote: ... and SVVESVKFL+7 were synthesized at the MIT-Koch Institute Swanson Biotechnology Center in Biopolymers and Proteomics Facility using standard Fmoc chemistry using an Intavis model MultiPep peptide synthesizer with HATU activation and 5 μmol chemistry cycles as previously described.(24) Standard Fmoc amino acids were procured from NovaBiochem and Fmoc-Leu (13C6, 15N) was obtained from Cambridge Isotope Laboratories ...
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bioRxiv - Microbiology 2020Quote: ... irregularis was cultured in modified SC solid medium supplied with 1 mM neutralized [1-13C]myristic acid (Cambridge Isotope Laboratories, Inc.) for 2.5 months ...
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bioRxiv - Biochemistry 2021Quote: ... Extraction solution contained 80:20 methanol:water with 1:2500 dilution of a heavy amino acid mix standard (MSK-A2, Cambridge Isotope Laboratories); derivatization solution is described below ...
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bioRxiv - Cell Biology 2021Quote: ... that cannot form lysine-linked polySUMO chains were grown for at least ten divisions in synthetic complete media supplemented either with unlabeled (Lys0 and Arg0; light) or heavy isotope-labeled amino acids (Lys8 and Arg10; heavy) from Cambridge Isotope Laboratories ...
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bioRxiv - Molecular Biology 2022Quote: ... medium (L-arginine:HCL [U13C6] and L-lysine:2HCL [4,4,5,5-D4], or heavy (L-arginine:HCL (13C6, 15N4) and L-lysine:2HCL (13C6, 15N2)) amino acid isotopes (Cambridge Isotope Laboratories Inc.).
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bioRxiv - Cell Biology 2024Quote: ... SRM transitions were detailed in the TraceFinder software and then verified with the Metabolomics Amino Acid Mix Standard (Cambridge Isotope Laboratories) for unique transitions ...
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bioRxiv - Cancer Biology 2023Quote: GMP clones were lysed in 100 µl ice-cold 80% methanol in water and polar metabolites were extracted using methanol-chloroform phase separation (1 ml methanol containing 2.5 µM of an internal standard (13C-, 15N-labeled amino acid mix; Cambridge Isotope Laboratories), 500 µl water ...
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bioRxiv - Cancer Biology 2023Quote: ... Two transitions were optimised using an authentic standard of glycocholic acid (glycine-1-13C, CLM-191-PK, Cambridge Isotope Laobratories, UK), from the negative precursor ion (m/z 465 ...
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bioRxiv - Biochemistry 2023Quote: RPE cells were incubated in pH 7.4 α-MEM and 50 µM [U-13C16]-palmitic acid (Cambridge isotope labs, CLM-409) complexed to bovine serum albumin at a 6:1 ratio of fatty acid to albumin ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were washed three times with 1 ml of cold 0.9% NaCl and scraped into 500µL of cold 80%v/v LC-MS grade methanol containing heavy amino acid internal standards (Cambridge Isotope Laboratories). After 10 minutes of vortexing at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Chromatographic performance was quality controlled using a mixture of unlabeled standard amino acids and a mixture of chemically diverse compounds (Cambridge Isotope Laboratories ...
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bioRxiv - Cell Biology 2023Quote: ... of 80% methanol in LC–MS water containing 500 nM isotope-labelled amino acids used as internal standards (Cambridge Isotope Laboratories). Following extraction ...
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bioRxiv - Systems Biology 2024Quote: ... and either heavy (13C615N4 L-arginine or Arg10 an 13C615N2 L-lysine or Lys8) and light amino acids (Cambridge Isotope Laboratories) at 0.4 mmol/L and 0.8 mmol/L ...
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bioRxiv - Neuroscience 2024Quote: ... with 0.15% formic acid) containing 10 μM of [13C-U] [15N-U] labelled internal standards (MSK-CAA-1; Cambridge Isotope Laboratories). Analytes were separated using a SeQuant ZIC cHILIC 3μm 100Å 150 x 2.1 mm column (EMD Millipore ...
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bioRxiv - Systems Biology 2024Quote: ... 20% LC-MS water) and supplemented with isotopically labelled internal standards (17 amino acids and isotopically labelled reduced glutathione, Cambridge Isotope Laboratories ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were washed with 0.9% NaCl twice and lysed with pre-chilled extraction buffer that was composed of 480 ul of MeOH mixed with 120 ul of H2O and 1 uM of pre-mixed Heavy Amino Acid mix (Cambridge Isotope Laboratories). Lysates were transferred to an Eppendorf tube and vortexed at 4 °C for 10 min before being centrifuged at 16,000 g at 4 °C for 10 min ...
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bioRxiv - Cell Biology 2020Quote: ... L amino acids were substituted for the same concentrations of heavy isotope–labeled (H) amino acids 13C6-15N2 L-Lys:2HCl and 13C6-15N4 L-Arg (Cambridge Isotope Laboratories). For immature dendritic cells (Dc ...
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bioRxiv - Cell Biology 2020Quote: Cells were washed twice with ice cold PBS and extracted on dry-ice in 0.8 mL 80% methanol containing 500 nM internal standards (Metabolomics Amino Acid Mix Standard: Cambridge Isotope Laboratories, Inc.). Cell extracts were collected using a cell scraper and transferred to a microcentrifuge tube ...
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bioRxiv - Cell Biology 2022Quote: ... Polar metabolites were extracted in 80 % methanol containing 15N and 13C fully-labeled amino acid standards (MSK-A2-1.2, Cambridge Isotope Laboratories, Inc). Extracts were shaken for 10 min with a vortexer ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% FBS and heavy isotope-labeled amino acids (L-Lysine 13C6, L-Arginine 13C6) (CLM-2247-H-1, CLM-2265-H-1, Cambridge Isotope Laboratories). DAC-treated cells grown in the regular medium were considered as SILAC light counterparts.
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bioRxiv - Cell Biology 2021Quote: ... and 500 μl of ice-cold 80% methanol in water with 250nM 13C/15N labeled amino acid standards (MSK-A2-1.2: Cambridge Isotope Laboratories, Inc.) was added to each well ...
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bioRxiv - Biochemistry 2020Quote: ... and 0.798 mM L-(13C6,15N2)-lysine (Lys“8”)] or “medium” amino acids [0.398 mM L-(15N4)-arginine (Arg“4”) and 0.798 mM L-(D4)-lysine (Lys“4”)] (Cambridge Isotope Laboratories, Tewksbury, USA), and incubated for 2 h ...
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bioRxiv - Cell Biology 2021Quote: ... which were replaced with either unlabelled amino-acids (Lys0 and Arg0) or stable isotopic forms 13C6 15N2-lysine (Lys8) and 13C6 15N4-arginine (Arg10) (Cambridge Isotope Laboratories). The medium was supplemented with 10% dialyzed FBS using previous published methods (Golebiowski et al ...