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Citations for Cambridge Isotope Labs :
51 - 93 of 93 citations for 6 6' Di O tert butyldimethylsilyl D lactal since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Isotope tracing experiments were performed using heavy D-13C6-Glucose (Cambridge Isotope Laboratories, #CLM-1396). Cells were seeded in 6-well plates ...
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bioRxiv - Cell Biology 2022Quote: ... Labelled media refers to EMM with 2% D-Glucose (U-13C6) (Cambridge Isotope Laboratories, Inc.). Once cells came into contact with labelled media ...
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bioRxiv - Bioengineering 2022Quote: ... 100 μl of 5 M isotope labelled D-Glucose (U-13C6, 99% from Cambridge Isotope Laboratories) prior to feeding ...
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bioRxiv - Biochemistry 2024Quote: ... and 100 mg/L 2-keto-3-[D]-[13CH3,12CD3]- isovalerate (Cambridge Isotope Laboratories, Inc. CDLM-7317) to enable selective labeling of ILV methyl groups ...
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bioRxiv - Biophysics 2021Quote: ... 2 g/l of U-[2H,13C] D-glucose and 1 g/l 15NH4Cl (Cambridge Isotope Laboratories). For production of U-[2H ...
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bioRxiv - Biochemistry 2020Quote: ... retinas were placed in pre-warmed KRB containing D-[U-13C]-glucose (Cambridge Isotope Laboratories, CLM-1396), D-[U-13C]-succinate (Cambridge Isotope Laboratories ...
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bioRxiv - Bioengineering 2020Quote: C13 pyruvate (CLM-1082-PK) and C13 D-glucose (U-13C6, 99%) were purchased from Cambridge Isotope Laboratories ...
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bioRxiv - Cancer Biology 2024Quote: ... and then switched into normal DMEM containing 4.5 mg/mL D-Glucose (DLM-2062, Cambridge Isotope Laboratories) + 1mM Sodium Pyruvate (Catalog # ...
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bioRxiv - Cancer Biology 2021Quote: ... all tested cells were treated with DMEM containing 25mM D-Glucose U-13C6 (Cambridge Isotope Laboratories (Tewksbury, MA))and 2% BSA under 37°C/5% CO2 for 24 hours or indicated time points for flux rate tests ...
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bioRxiv - Zoology 2021Quote: ... we added 25% (w/w) of the stable isotope-labelled precursors [13C6] D-glucose (Cambridge Isotope Laboratories, Inc.) as well as a control with untreated wheat grass ...
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bioRxiv - Microbiology 2023Quote: ... Biomass was scraped into 20ml flask of Celtone-R4 broth (10 g D-glucose; Cambridge Isotope Laboratories (CLM) #CLM-1396-5 ...
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bioRxiv - Neuroscience 2020Quote: ... mice were IP-injected with 500 mg/kg 13C-labeled D-glucose (U-13C6, 99%, Cambridge Isotope Laboratories Inc.). Retinas were rapidly collected 45 minutes after injection using an established technique 8 in 200 µl cold HBSS ...
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bioRxiv - Cancer Biology 2022Quote: ... Dried pellet from unlabeled samples was mixed with [U13C]-D/L-2HG (internal standard for unlabeled samples, Cambridge isotope laboratories ...
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bioRxiv - Biochemistry 2024Quote: ... The culture was diluted one-to-one with M9/D2O medium (prepared with D-[2H;12C]-glucose, Cambridge Isotope Laboratories ...
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bioRxiv - Molecular Biology 2024Quote: EGM-2 media was replaced with DMEM media containing D-Glucose (U-13C6, CLM-1396-PK, Cambridge Isotope Laboratories Inc.) 3 hours prior to collection ...
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bioRxiv - Molecular Biology 2022Quote: ... supplemented with 15NH4Cl and when needed 13C-D-glucose as sole nitrogen and carbon sources (purchased from Cambridge Isotope Laboratories). The cells were grown at 37°C before they were induced with 0.2 mM IPTG at an OD600 of 1.2 for TB and 0.8 for M9 minimal medium and incubated at 17°C for 16 h.
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bioRxiv - Molecular Biology 2024Quote: ... 15N labeled protein was expressed in a modified M9 medium containing D-glucose (Cambridge Isotope Laboratories, Inc., U-13C6, 99%) and ammonium chloride (Cambridge Isotope Laboratories ...
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bioRxiv - Biochemistry 2022Quote: ... Cell pellets were resuspended in 250 mL of isotopically labelled minimal media M9 salt solution supplemented with 13C D-glucose 4.0 g/L and 15NH4Cl 1.0 g/L (Cambridge Isotope Laboratories, Inc.), then incubated to allow the recovery of growth and clearance of unlabeled metabolites ...
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bioRxiv - Molecular Biology 2020Quote: ... cultures were first grown at 37°C in 1 L of LB media until an OD600 of 0.6 before the cells were pelleted and resuspended in 0.5 L M9 minimal media prepared in D2O supplemented with 13C-D-glucose and 15NH4Cl (Cambridge Isotope Laboratories). After 1 hour recovery at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... G7021 and for labeled glucose we used either D-glucose-U-13C (99%, Cambridge Isotope Laboratories, cat no CLM-1396-10) or D-glucose-2-13C glucose (99% ...
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bioRxiv - Biochemistry 2021Quote: ... 450 μL of supernatant were transferred to a new tube and 150 μL of deuterated water were added (D2O, D, 99.9%, Cambridge Isotope Laboratories). Each sample was vortex-mixed for 1 min before transferring into 5 mm SampleJet NMR tubes ...
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bioRxiv - Microbiology 2023Quote: When the protein samples were prepared for NMR analysis the bacteria were cultured in M9 media containing D-[13C] glucose (U-13C6, 99%, CLM-1396-1, Cambridge Isotope) and 15NH4Cl (15NH4 Chloride ...
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bioRxiv - Neuroscience 2023Quote: ... Experiments with deuterated lipids were conducted by replacing glucose with 10mM D-glucose (U-13C6, Cambridge isotope laboratories CLM-1396-PK) for 24h hours prior to treatment with 5 μM Aβ fibrils or no treatment.
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bioRxiv - Biophysics 2024Quote: ... 4.0 gL-1 of D-glucose provided in specific isotopic ratios (12C, Merek, Germany; 13C, Cambridge Isotope Laboratories, USA; Table S1). 109 cells were harvested at late-exponential growth phase (Figure S3) ...
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bioRxiv - Cancer Biology 2023Quote: U-[13C]-Glc labelling media consisted of DMEM supplemented with 10 mM D-glucose (U-13C6; CLM-1396; Cambridge Isotope Laboratories), 2 mM glutamine ...
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bioRxiv - Biophysics 2022Quote: HDX samples of I552A SLO-1 were exchanged as a function of time by a 10-fold dilution into D2O (99% D; Cambridge Isotope) buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... no-glucose and no-glutamine media was supplemented with 5 mM of 13C6 D-Glucose (Cambridge Isotope Laboratories, Inc., CLM-1396) and 4 mM unlabeled glutamine ...
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bioRxiv - Molecular Biology 2021Quote: ... expression was carried out in M9 minimal media supplemented with 3 g/L (13C6, 99%)-D-glucose and/or 1 g/l (15N, 99%)-NH4Cl (Cambridge Isotope Laboratories). To generate isotopically labeled rad6-A126T ...
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bioRxiv - Biophysics 2021Quote: ... Deuterated RNA samples were prepared in the same way using selective deuterated rNTPs (D-H5, H3′, H4′, H5′ and H5′′) and 13C/15N-labeled samples were prepared using labeled rNTPs (Cambridge Isotope Laboratories). The resulting RNAs were purified for NMR studies by gel electrophoresis ...
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bioRxiv - Microbiology 2021Quote: ... and Rpk9-I53-50A trimers were H/D exchanged (HDX) in the deuteration buffer (pH* 7.5, 85% D2O, Cambridge Isotope Laboratories, Inc.) for 3 ...
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bioRxiv - Immunology 2020Quote: 3 µg of monomeric RBD and RBD-8GS-I53-50A were incubated and H/D exchanged (HDX) in the deuteration buffer (pH* 7.6, 85% D2O, Cambridge Isotope Laboratories, Inc.) for 3 ...
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bioRxiv - Pathology 2024Quote: ... and control uninfected mice were injected intraperitoneally with either 1 g/kg of 13C6-labeled D-glucose (CLM-1396-1, Cambridge Isotope Laboratories) dissolved in sterile PBS or 0.5 mg/gram of body weight 13C3 Sodium L-lactate (CLM-1579-0.5 ...
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bioRxiv - Microbiology 2021Quote: ... uniformly 15 N- and 13 C-labeled proteins were produced by growing cell cultures in M9 minimal medium that contained 1 g/l 15 N-ammonium chloride and 2 g/l 13 C-D-glucose (Cambridge Isotope Laboratories, Inc.) as the sources of nitrogen and carbon ...
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bioRxiv - Biochemistry 2021Quote: ... 13C-1 labelled polyM was produced from fermentation of the same strain using 13C-1 (99 % 13C) D-fructose (Cambridge Isotope Laboratories, USA). This substrate was further subjected to acid hydrolysis as described elsewhere (39 ...
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bioRxiv - Molecular Biology 2020Quote: ... Unlabeled samples were prepared using Luria Broth (LB) (Melford) and minimal media M9 with 15NH4Cl and/or D- [13C] glucose (Cambridge Isotope Laboratories, Inc) were used to prepare the labeled samples (25) ...
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bioRxiv - Biophysics 2024Quote: ... The cells were used to inoculate 2L of H2O-based M9 media supplemented with 3 g L-1 of [U-13C6] d-glucose (Cambridge Isotope Laboratories, CIL) and 2 g L-1 of [15N] ammonium chloride (Cambridge Isotope Laboratories ...
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bioRxiv - Cell Biology 2024Quote: ... grown in monolayers were switched to glucose free media for 3 hours and incubated in 5mm D-glucose (unlabeled) or 13C6- glucose (Cambridge Isotope Laboratories, Inc) for 6 hours ...
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bioRxiv - Biophysics 2024Quote: ... The 1 L M9 culture contained 1 g of 15N labelled ammonium chloride and 2 g of 13C uniformly labelled α-D glucose (Cambridge Isotope Laboratories). The M9 culture was incubated for 30 min with shaking at 37 °C and 220 RPM before adding IPTG to 0.5 mM ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were prepared using 500uL of sample mixed with 500uL of an NMR buffer containing a chemical shift reference and analytical standard (TSP-2, 2, 3, 3-d4 (D,98% Sodium-3-Trimethylsilyl-Propionate, Cambridge Isotope Laboratories, Inc.). The NMR buffer was prepared with Mono- and Dibasic Potassium Phosphate at 0.5M concentration and a pH of 7 ...
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bioRxiv - Biophysics 2023Quote: ... The exo-PS accumulated on the 10 kDa membrane surface as a clear gel and was collected and dialyzed extensively against MilliQ-grade H2O or D2O (Cambridge Isotope Labs, 99.9% D). The H2O solutions provide scattering profiles at an additional contrast ...
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bioRxiv - Biophysics 2023Quote: ... Uniformly-labeled 13C 15N Sup35NM samples were prepared by growing BL21(DES)-Rosetta Escherichia coli in the presence of M9 media with 2 g L-1 D-glucose 1H,13C6 and 1 g L-1 of 15N ammonium chloride (Cambridge Isotope Labs, Cambridge, MA). Purified ...
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Metabolic function in aging retina and retinal pigment epithelium remains robust despite vision lossbioRxiv - Biochemistry 2021Quote: Either 5 mM U-13C glucose alone or 5 mM unlabeled glucose and 2 mM U-13C glutamine U-13C metabolic tracers (99% isotopic purity) included 13C labeled D-Glucose and L-Glutamine (Cambridge Isotope Laboratories, Inc; Tewksbury, MA). Incubations ...
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bioRxiv - Microbiology 2022Quote: ... DNA with different levels of 13C enrichment was produced by varying the ratio of unlabeled glucose to uniformly-labeled 13C6-D-glucose (Cambridge Isotope Laboratories; CLM-1396; 99 atom %), e.g ...