Labshake search
Citations for Cambridge Isotope Labs :
51 - 100 of 1125 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2024Quote: ... 15N]-GSH (CNLM-6245-50) were purchased from Cambridge Isotope Laboratory ...
-
bioRxiv - Genetics 2024Quote: ... Polar metabolites were extracted in 500μL ice-cold 80% methanol containing amino acid standards (Cambridge Isotope Labs, MSK-A2-1.2). Samples were vortexed at 4°C for 10 minutes and centrifuged for 10 minutes at 20000xg at 4°C before supernatant was collected and dried via nitrogen evaporation ...
-
bioRxiv - Immunology 2024Quote: ... and labeled (U-13C6, Cambridge Isotope Laboratories CLM-1396-1) or unlabeled glucose (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2024Quote: ... Labeled ([3-13C]-Lactate, Cambridge Isotope Laboratories CLM-1578) or unlabeled lactate (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2024Quote: ... 10 µL supernatant was added 40 µL solution of stable isotope-labeled internal standards (phenylalanine-D5, tyrosine-D4, valine-D8, methionine-D3, lysine-13C6-15N2, and arginine 13C6-15N4; Cambridge Isotope Laboratories) and 180 µL solvent (water with 0.2% heptafluorobutyric acid) ...
-
bioRxiv - Biochemistry 2024Quote: ... and deuterated Celtone base powder (Cambridge Isotope). The 2H ...
-
bioRxiv - Biochemistry 2024Quote: ... NMR solvents were purchased from Cambridge Isotope Laboratories ...
-
bioRxiv - Biochemistry 2024Quote: ... 13C-depleted glucose was purchased from Cambridge Isotope Laboratories and 15N-depleted (NH4)2SO4 was from Merck ...
-
bioRxiv - Biophysics 2024Quote: ... and 2 g L-1 of [15N] ammonium chloride (Cambridge Isotope Laboratories, CIL) as the sole carbon and nitrogen source ...
-
bioRxiv - Biophysics 2024Quote: ... The cells were used to inoculate 2L of H2O-based M9 media supplemented with 3 g L-1 of [U-13C6] d-glucose (Cambridge Isotope Laboratories, CIL) and 2 g L-1 of [15N] ammonium chloride (Cambridge Isotope Laboratories ...
-
bioRxiv - Biochemistry 2024Quote: ... The buffer was first prepared in ultrapure water and then redissolved in an equivalent volume of 99.96% D2O (Cambridge Isotope Laboratories, Inc., Andover, MA) just prior to use ...
-
bioRxiv - Biochemistry 2024Quote: ... and L-arginine:HCl (Cambridge isotope # CNLM-539-H).
-
bioRxiv - Biochemistry 2024Quote: ... Heavy arginine and lysine contain the isotopes 13C and 15N: L-lysine:2HCl (Cambridge Isotope #CNLM-291-H) and L-arginine:HCl (Cambridge isotope # CNLM-539-H).
-
bioRxiv - Biophysics 2024Quote: ... 4.0 gL-1 of D-glucose provided in specific isotopic ratios (12C, Merek, Germany; 13C, Cambridge Isotope Laboratories, USA; Table S1). 109 cells were harvested at late-exponential growth phase (Figure S3) ...
-
Sex-specific fear acquisition following early life stress is linked to amygdala glutamate metabolismbioRxiv - Animal Behavior and Cognition 2024Quote: ... U-13C15N-labeled amino acids at a final concentration of 0.25 µM (prepared as 2.5 mM in 0.1 N HCl, Cambridge Isotope Laboratories, MSK-A2-1.2), citric acid d4 at 0.02 µg/mL (dissolved at 100 µg/mL in H2O ...
-
bioRxiv - Biochemistry 2024Quote: ... and 2 g/L U-13C6-Glucose (Cambridge Isotope Laboratories) as the sole nitrogen and carbon sources ...
-
bioRxiv - Biochemistry 2024Quote: ... Isotopes were purchased from Cambridge Isotope Laboratories ...
-
bioRxiv - Cancer Biology 2024Quote: ... the media was removed and replaced with glutamine-free media supplemented with 10% dFBS and 4 mmol/L U-13C5-L-glutamine (CLM-1822-H, Cambridge Isotope Laboratories) for 2 hours prior to harvest (n = 3 per condition) ...
-
bioRxiv - Cancer Biology 2024Quote: ... Samples were quenched in 1 mL pre-chilled −80°C 80/20 methanol:water (v/v) and spiked with 50µL 1µM isotope labeled TCA cycle mix (Cambridge Isotope Laboratories MSK-TCA-A) pre-diluted in 80/20 methanol:water and 50µL 0.02 ng/µL Propionyl-L-carnitine-(N-methyl-d3 ...
-
bioRxiv - Cancer Biology 2024Quote: ... supplemented with 1 g/L [U-13C]-glucose (Cambridge Isotope Laboratories, #110187-42-3), 10% dialyzed fetal bovine serum (R&D Systems ...
-
bioRxiv - Cancer Biology 2024Quote: ... BT20 and MDAMB157 were labeled with 13C6 Lys (#CNLM-2247, Cambridge isotope laboratories) in RPMI1640 containing 10% dialyzed FBS and 1 % penicillin/streptomycin cocktail (Gibco) ...
-
bioRxiv - Biophysics 2024Quote: ... to obtain the 15N uniformly single labelled protein or in a media containing 15NH4Cl (0.1% w/v) and 13C-labelled glucose (0.4% w/v) (Cambridge isotope Laboratories, Inc.) for 15N and 13C uniformly double labelled protein ...
-
bioRxiv - Biophysics 2024Quote: ... 1 L cultures of autoinduction media (Cold Spring Harbor Protocols) were used with 15N labeled ammonium chloride (Cambridge Isotope Laboratories). The final antibiotic concentration was increased to 0.1 mg/mL of kanamycin to reduce plasmid loss and poor yields ...
-
bioRxiv - Biophysics 2024Quote: ... 10.0 g/L 13C-glucose (CLM-1396-PK, Cambridge Isotope Laboratories) and 6.0 g/L15N-(NH4)2SO4 (NLM-713-PK ...
-
bioRxiv - Biophysics 2024Quote: ... and 6.0 g/L15N-(NH4)2SO4 (NLM-713-PK, Cambridge Isotope Laboratories), with the pH adjusted to 7.0 ...
-
bioRxiv - Biophysics 2024Quote: ... the cells were transferred to fresh LB medium for the overexpression of the unlabeled protein or to M9 minimal medium supplied with 15NH4Cl (Cambridge Isotope Laboratories Inc.) as the only nitrogen source for the labeled protein overexpression ...
-
bioRxiv - Cell Biology 2024Quote: ... SRM transitions were detailed in the TraceFinder software and then verified with the Metabolomics Amino Acid Mix Standard (Cambridge Isotope Laboratories) for unique transitions ...
-
bioRxiv - Cell Biology 2024Quote: ... 13C6-Glucose or 13C3-pyruvate were added for either the full 48 hours or in the last 8 hours accompanied by a media change (Cambridge Isotope Laboratories). Cells were then washed with ice-cold PBS three times and metabolites extracted using an extraction solvent consisting of 50% methanol ...
-
bioRxiv - Systems Biology 2024Quote: ... 10 µL of spent media samples were mixed with 10 µL of known concentration of isotopically labeled internal standards of amino acids (Cambridge Isotope Laboratory, Andover, MA, MSK-A2-1.2). 600 µL of ice cold HPLC grade methanol was added ...
-
bioRxiv - Plant Biology 2024Quote: ... The dimethylation reaction was carried out by adding 2 µL of a 4% formaldehyde solution (Formaldehyde 13C, d2 solution, 596388, Sigma-Aldrich or Formaldehyde-D2, DLM-805-PK, Cambridge Isotope Laboratories Inc.) and 2 µL of a 500 mM NaCNBH3 solution per 100 µL sample at 37°C for 4 h ...
-
bioRxiv - Biophysics 2024Quote: ... The 1 L M9 culture contained 1 g of 15N labelled ammonium chloride and 2 g of 13C uniformly labelled α-D glucose (Cambridge Isotope Laboratories). The M9 culture was incubated for 30 min with shaking at 37 °C and 220 RPM before adding IPTG to 0.5 mM ...
-
bioRxiv - Immunology 2024Quote: ... For 13C-[U]-Glutamine (#CLM-1822, Cambridge Isotope Laboratories) tracing ...
-
bioRxiv - Microbiology 2024Quote: ... or 50% 2H2O (99.9%-2H; Cambridge Isotope Laboratories). The optical density (600 nm ...
-
bioRxiv - Microbiology 2024Quote: ... A single colony was then used to inoculate 100 mL of fresh M9 medium (Helmholtz Center Munich, see Supplementary Information S1) containing 0.4 % 13C-glucose (Cambridge Isotope Laboratories Inc, USA). The inoculated liquid culture was incubated in a 250mL baffled Erlenmeyer flask on shaker at 150 rpm at 37 °C for 24 h ...
-
bioRxiv - Cell Biology 2024Quote: ... supplemented with 10% (v/v) dialyzed fetal bovine serum, 600mg/l proline, 42mg/l arginine hydrochloride (or 13C6, 15N4-arginine in ‘heavy’ media) (Cambridge Isotope Laboratories) and 146mg/l lysine hydrochloride (or 13C6 ...
-
bioRxiv - Physiology 2024Quote: Metabolomics was performed on microglia by first washing microglia primary cell cultures with PBS and re-cultured in DMEM (lacking glucose) containing 10% dialyzed FBS and uniformly labeled [13C]-Glucose (Cambridge Isotope Laboratories). Microglia (2 x 106 per well in 6 well plates ...
-
bioRxiv - Immunology 2024Quote: ... and provided DMEM described above except with 25mM 13C6-glucose (Cambridge Isotope Laboratories, CLM-1396-PK) to competitively label metabolites between conditions as a proxy for metabolic pathway activity ...
-
bioRxiv - Biochemistry 2024Quote: ... Fmoc-protected amino acids were obtained from Cambridge Isotope Laboratories and used without further modification ...
-
bioRxiv - Microbiology 2024Quote: ... Data were normalized by a 13C stable isotope labeled internal standard (Cambridge Isotope Laboratories, Inc., Tewksbury, MA). The results were quantified using a standard curve with concentrations ranging from 0.78 μM to 50 μM ...
-
bioRxiv - Cancer Biology 2024Quote: ... including 30µM 13C9,15N2-uridine (Cambridge Isotope Laboratories Inc), or unlabelled equivalent (Sigma) ...
-
bioRxiv - Cancer Biology 2024Quote: ... [U-13C]-18:2(n-6) (Cambridge Isotope Laboratories, CLM-6855-PK) was supplemented as bovine serum albumin-fatty acid (BSA-FA ...
-
bioRxiv - Immunology 2024Quote: ... 15N(amino)-glutamine (NLM-1016, Cambridge Isotope Laboratories) (2mM) ...
-
bioRxiv - Immunology 2024Quote: ... or 13C5-glutamine (CLM-1822, Cambridge Isotope Laboratories) (2mM ...
-
bioRxiv - Immunology 2024Quote: ... 15N(amide)-glutamine (NLM-557, Cambridge Isotope Laboratories) (2mM) ...
-
bioRxiv - Biochemistry 2024Quote: ... 15N labelled amino acid standard (Cambridge Isotope Laboratories, MSK-CAA-1) was spiked into samples at the first step of the extraction ...
-
bioRxiv - Cell Biology 2024Quote: ... larvae were incubated for 4.5 h in either labeled (4 mM NaH13CO3 sodium bicarbonate; Cambridge Isotope Laboratories, Tewksbury MA USA), unlabeled (4mM NaHCO3 sodium bicarbonate) ...
-
bioRxiv - Biochemistry 2024Quote: ... [13C6]D-Glucose (#CLM-1396-1) was purchased from Cambridge Isotope Laboratories ...
-
bioRxiv - Neuroscience 2024Quote: ... NeuCode L-lysine:2HCL(3,3,4,4,5,5,6,6-D8) or L-Lysine:2HCL(13C6; 15N2) (Cambridge Isotope Laboratories) were used to label either wildtype or knockout cells ...
-
bioRxiv - Physiology 2024Quote: Mice were fasted for 2 hours then injected intraperitoneally (i.p.) with 1g/kg uniformly labelled U-13C-glucose (CLM-1396, Cambridge Isotope Labs) in saline ...
-
bioRxiv - Neuroscience 2024Quote: ... while heavy isotope labelled GSH (glycine-13C2, 15N-Glutathione) (GSH*) was purchased from Cambridge Isotope (UK). All of these reagents were resuspended in water to make a stock of 1 mg/mL for each and used as standards and internal standards (GSH*) ...