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Citations for Cambridge Isotope Labs :
501 - 550 of 1125 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... and 10 mM 13C-labeled glucose (Cambridge Isotope Laboratories). Cells were washed with ice-cold saline and snap frozen on dry-ice and stored at −80°C.
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bioRxiv - Cell Biology 2022Quote: ... 15N2 l-lysine (heavy SCD, Cambridge Isotope Laboratories, 25 mg/L).
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bioRxiv - Biochemistry 2022Quote: ... and 0.798 mM L-(13C6,15N2)-lysine (Lys”8”)] or “medium-heavy” amino acids [0.398 mM L-(13C6)-arginine (Arg”6”) and 0.798 mM L-(D4)-lysine (Lys”4”)] (Cambridge Isotope Laboratories, Tewksbury, USA). For chloramphenicol (CAP ...
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bioRxiv - Microbiology 2022Quote: ... were resuspended in 300 μL of LC/MS grade methanol containing a mixture of 17 isotope-labeled amino acids (200 nM, Cambridge Isotope Laboratories, MSK-A2-1.2). To this solution ...
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bioRxiv - Microbiology 2022Quote: ... and 50 nmol of DSS-d6 (Cambridge Isotope Laboratories) was added to each sample for chemical shift referencing ...
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bioRxiv - Systems Biology 2022Quote: ... and methionine (Cambridge Isotope Laboratories, Inc.) and analyzed by LC-MS using a Waters BEH Amide 150 × 2.1 mm ...
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bioRxiv - Systems Biology 2022Quote: ... non-polar samples were reconstituted in CDCl3 (99.96%; Cambridge Isotope Laboratories, Inc.). Samples were vortexed until fully soluble ...
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bioRxiv - Systems Biology 2022Quote: ... polar samples were reconstituted in D2O (99%, Cambridge Isotope Laboratories, Inc.) in a 100 mM sodium phosphate buffered solution with 0.11 mM sodium 2,2-dimethyl-2-silapentane-5-sulfonate (DSS-D6 ...
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bioRxiv - Systems Biology 2022Quote: ... in a 100 mM sodium phosphate buffered solution with 0.11 mM sodium 2,2-dimethyl-2-silapentane-5-sulfonate (DSS-D6; 98%; Cambridge Isotope Laboratories, Inc.). Once dry ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 600 μL of DMSO-d6 containing 0.3% tetramethylsilane (Cambridge Isotope Laboratories Inc. Cat#DLM-10TC-25), and 10 μL of a 306 mM solution of 1,2,4,5-tetrachloro-3-nitrobenzene in DMSO-d6 were combined ...
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bioRxiv - Cell Biology 2022Quote: ... Metabolites were extracted with 1 mL 80% MeOH pre-cooled to −80 °C containing 10 nmol [D8]-valine as an internal standard (Cambridge Isotope Labs). Insoluble material was removed by centrifugation at 21,000 ×g for 15 min at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... the dried pellet was dissolved in 550 µl D2O containing 0.01 mg/ml Sodium 2,2-Dimethyl-2-Silapentane-5-Sulfonate (DSS; Cambridge Isotope). The samples were then vortexed and centrifuged at 16000 rpm for 12 min ...
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bioRxiv - Immunology 2022Quote: ... of the samples was used to scale the metabolite extraction to a ratio of 1×106 cells / 1 mL extraction solution [80% LCMS grade methanol (aq) with 500nM labelled amino acid internal standard (Cambridge Isotope Laboratories, Inc., Cat No. MSK-A2-1.2)] ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by treatment with 12.5μM PA-d31 (Cambridge Isotope Lab) for 24 hours ...
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bioRxiv - Biochemistry 2022Quote: ... 13C-glucose (Cambridge Isotope Laboratories) was used in medium for expression of 15N ...
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bioRxiv - Microbiology 2022Quote: ... and d4-palmitic acid (d4-C16:0, 98%, Cambridge Isotope Laboratories, DLM-2893) were used as injection standards for ultra-performance LC-HRMS (UPLC-HRMS ...
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bioRxiv - Biochemistry 2022Quote: ... uniformly labelled 13C-glucose was used (#CLM-1396, Cambridge Isotope Laboratories Inc., USA). The M9 precultures were grown overnight at 37°C and at 220 rpm shaking ...
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bioRxiv - Plant Biology 2022Quote: ... [13C]-labeled sodium bicarbonate (Cambridge Isotope Laboratories, Inc.: 99% 13C) was added to the culture.
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bioRxiv - Synthetic Biology 2022Quote: ... 13CO2 was obtained from Cambridge Isotope Laboratories (Andover ...
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bioRxiv - Cell Biology 2022Quote: ... and 13C6 15N2 l-lysine (175 mg/liter) (Cambridge Isotope Laboratories). Experiments were conducted when incorporation rate of labelled amino acids was superior to 95% ...
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bioRxiv - Immunology 2022Quote: ... and re-cultured (2.5 × 106 cells/well in 24-well plates) for indicated times in medium containing 13C-labeled metabolites (Cambridge Isotope Laboratories) at the following concentrations ...
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bioRxiv - Immunology 2022Quote: ... and [13C4]-ethyl-acetoacetate (Cambridge Isotope labs) were hydrolysed by mixing 1ml of ethyl acetoacetate with 8 ml 1M NaOH at 60°C by stirring for 30 min ...
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bioRxiv - Microbiology 2022Quote: ... 99% [2,3-13C] sodium succinate or an equimolar molar 1:1 mixture of [U-13C] sodium succinate (obtained from Cambridge Isotope Laboratories, Inc., Andover, MA, USA) and naturally labelled sodium succinate.
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bioRxiv - Microbiology 2022Quote: ... supplemented with 10 mM 13C6-glucose (Cambridge Isotope Laboratories, CLM-1396-2) and dialyzed FBS (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Fmoc-Glu (13C5 15N) were obtained from Cambridge Isotope Laboratories ...
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bioRxiv - Cancer Biology 2022Quote: ... 1×106 cells were plated in medium supplemented with 17.5 mM glucose (unlabeled glucose or 13C6-glucose [CLM-1396, Cambridge Isotope Laboratories]) or 2 mM glutamine (unlabeled glutamine or 13C5-glutamine [CLM-1822] ...
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bioRxiv - Cancer Biology 2022Quote: 500k BMDMs were seeded on 50 mm2 varied stiffness ECM coated PA-gels and cultured for 24h in 25 mM glucose DMEM or 20-22 h in 25 mM glucose DMEM followed by medium change to DMEM lacking glutamine (Gibco, 11960044 replaced with with 13C5-glutamine (Cambridge Isotope Laboratories, CLM-1822-H-PK) for 2 h ...
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bioRxiv - Cancer Biology 2022Quote: ... replaced with 13C6-arginine (Cambridge Isotope Laboratories, CLM-2265-H-PK) and L-lysine (sigma ...
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bioRxiv - Developmental Biology 2022Quote: ... flies were fed for 12hrs a mixture of 0.5M 13C-glucose (Cambridge Isotope Laboratories, Inc #CLM-1396-1) and 0.5M unlabeled glucose (Sigma #G5400) ...
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bioRxiv - Plant Biology 2022Quote: ... six leaf discs from the same leaf were transferred in a well of a 6-well microplate filled with 4 mL/well of a buffer containing 10 mM MES-KOH (pH 6.5) and 10 mM U-13C-pyruvate (13C isotopic purity of 99%; sodium salt from Cambridge Isotope Laboratories (Eurisotop, France)) ...
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bioRxiv - Plant Biology 2022Quote: ... 2-13C-acetate and U-13C-acetate (13C isotopic purity of 99%, sodium salts from Cambridge Isotope Laboratories (Eurisotop, France); Figure 1) ...
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bioRxiv - Cancer Biology 2022Quote: ... Due to [U-13C16]palmitate tracer purity of 98% (Cat#. #CLM-409, Cambridge Isotope Laboratories, Inc), we also observed a low fraction of labeled M+15 as well as M+31 on deoxyDHCer m18:0/16:0 ...
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bioRxiv - Cancer Biology 2022Quote: ... the cell pellet was resuspended into 1 mL of ice-cold 2:2:1 MeOH: ACN: H2O (v/v/v) containing 10 μM stable isotope-labeled canonical amino acid mix (Cambridge Isotope Laboratories, Inc) as internal standards ...
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bioRxiv - Cancer Biology 2022Quote: ... which was used as the qualitative standard (Cambridge Isotope Laboratories, Inc). Optimized spray voltage was at 5.5 kV for positive and 4.2 kV for negative mode ...
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bioRxiv - Molecular Biology 2022Quote: ... medium (L-arginine:HCL [U13C6] and L-lysine:2HCL [4,4,5,5-D4], or heavy (L-arginine:HCL (13C6, 15N4) and L-lysine:2HCL (13C6, 15N2)) amino acid isotopes (Cambridge Isotope Laboratories Inc.).
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bioRxiv - Cancer Biology 2022Quote: ... All heavy isotope labeled amino acids (99% pure) were purchased from Cambridge Isotope Laboratories (Andover ...
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bioRxiv - Molecular Biology 2022Quote: ... All labelled amino acids were purchased from Cambridge Isotope Laboratories ...
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bioRxiv - Molecular Biology 2022Quote: ... and 60 mg/L of alpha-ketoisovaleric acid (13C5, 98%; 3-D1, 98%, Cambridge Isotope Laboratory). All the recombinant protein expressions were performed at 37°C during 4 hours in presence of 1 mM IPTG.
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bioRxiv - Molecular Biology 2022Quote: ... 100 mg/L of alpha-ketobutyric acid (methyl-13C, 99%; 3,3-D2, 98%, Cambridge Isotope Laboratory) and 60 mg/L of alpha-ketoisovaleric acid (13C5 ...
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bioRxiv - Plant Biology 2022Quote: ... pH 5.7) or 15N media (½ strength MS nutrient without nitrogen, 15NH 15NO [0.5 g/L, Cambridge Isotope Laboratory] ...
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bioRxiv - Biochemistry 2022Quote: ... 15N2 (Lys8, Cambridge Isotope Laboratories, #CNLM-291-H), and 0.398 mM “heavy” L-arginine-13C6 ...
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bioRxiv - Biochemistry 2022Quote: ... 15N4 (Arg10, Cambridge Isotope Laboratories, #CNLM-539-H) for SILAC heavy group or the same concentrations of “light” L-lysine (Lys0 ...
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bioRxiv - Bioengineering 2022Quote: ... 100 % 1-13C glucose (Cambridge Isotope Laboratories), or unlabelled glucose ...
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bioRxiv - Cell Biology 2022Quote: ... Polar metabolites were extracted in ice cold 80% LC/MS grade methanol containing 15N and 13C fully-labeled amino acid standards (Cambridge Isotope Laboratories #MSK-A2-1.2). Tissues were homogenized by Bead Ruptor 24 (Omni International ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed twice with ice-cold 0.9% NaCl and polar metabolites extracted in ice cold 80% LC/MS grade methanol containing 15N and 13C fully-labeled amino acid standards (Cambridge Isotope Laboratories #MSK-A2-1.2). Extracts were vigorously shaken by vortex for 10 minutes at 4°C and spun at 14,000 rpm at 4°C for 10 minutes to remove insoluble cell debris ...
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bioRxiv - Cell Biology 2022Quote: ... or fresh media with 3mg/L [1,2-13C2]Choline chloride (Cambridge Isotope Laboratories #CLM-548.01) (as indicated in figure legends) ...
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bioRxiv - Cell Biology 2022Quote: ... The second plate was cultured in medium SILAC media with the same composition as above except Arg0 and Lys0 were replaced by L-Arginine [13C6] HCl (Arg-6) and L-ysine-4,4,5,5-d4 (Lys-4) (Cambridge Isotope Laboratories). The third plate was cultured in heavy SILAC media with the same composition except arginine and lysine were replaced by L-Arginine [13C6,D7,15N4]HCl (Arg-17 ...
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bioRxiv - Cell Biology 2022Quote: ... The third plate was cultured in heavy SILAC media with the same composition except arginine and lysine were replaced by L-Arginine [13C6,D7,15N4]HCl (Arg-17) and L-Lysine [13C6,15N2]HCl (Lys-8) (Cambridge Isotope Laboratories). Cells were passaged and expanded in the corresponding SILAC media for 10 doublings.
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bioRxiv - Cell Biology 2022Quote: ... 50 µl of 2× kinase buffer containing 80 µM O18 ATP (OLM-7858-PK, Cambridge Isotope laboratories, Inc) and 1 µg of bacterial purified human PIP4Kα-GST was added and the reaction was incubated at 30 °C for 1 hour ...
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bioRxiv - Plant Biology 2022Quote: ... or 15N media (½ strength MS nutrient without nitrogen, 15NH 15NO [0.5 g/L, Cambridge Isotope Laboratory], K15NO3 [0.5 g/L, Cambridge Isotope Laboratory], pH 5.7) for 10 days ...