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Citations for BestGene :
51 - 69 of 69 citations for Human LACC1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... A mixture of both plasmids was injected into flies expressing Cas9 under nos regulatory sequences (#54591; BDSC) by BestGene. CRISPR edited lines were identified by the presence of DsRed eye fluorescence and the DsRed marker was removed by crossing to flies expressing cre recombinase (#1092 ...
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bioRxiv - Genetics 2022Quote: ... The gw chiRNA plasmid was injected into embryos of the y1 M(vas-Cas9)ZH2A w1118 strain (BestGene Inc.). Males or females carrying vas-Cas9 and a sgRNA transgene were crossed to W1118 ...
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bioRxiv - Cell Biology 2019Quote: ... UASp-CFP-Msps plasmid was injected into embryos for targeted insertion on chromosome 3 at ZH-96E by Bestgene, Inc.
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Activity-dependent capture of neuropeptide vesicles prepares clock neuron synapses for daily releasebioRxiv - Neuroscience 2023Quote: ... A plasmid for UAS-Kir2.1 without a GFP tag provided by Sean Sweeney (University of York) was used by Bestgene Inc ...
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bioRxiv - Cell Biology 2019Quote: ... The plasmid pCFD4-Pcs was injected into embryos of PBac{yellow[+]-attP-3B}VK00033 (Bloomington stock center #9750) by BestGene Inc ...
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bioRxiv - Neuroscience 2021Quote: The appropriate combination of gRNA and donor plasmids was mixed and injected into embryos of vasa-Cas9 strain (Bloomington #51323) by BestGene Inc ...
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bioRxiv - Developmental Biology 2022Quote: The plasmids obtained were injected into flies (BDSC 9744) which contain an attP-9A insertion at 3R chromosome (89E11) by BestGene.
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bioRxiv - Developmental Biology 2021Quote: The robo2 gRNA plasmid was co-injected with the robo2robo2 or robo2robo2ΔIg1 homologous donor plasmids into nos-Cas9.P embryos (Bloomington Drosophila Stock Center stock #54591) (Port et al. 2014) by BestGene Inc (Chino Hills ...
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bioRxiv - Microbiology 2023Quote: ... Other original UAS transgenic lines listed below were generated using germline transformation by P-element based plasmid vectors (BestGene Inc.): UASp-Spaid.C290A-EGFP ...
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bioRxiv - Neuroscience 2023Quote: pFlpStop-attB-UAS-2.1-tdTom (addgene #88910) donor plasmid was injected into shakB[MI15228] flies together with φC31 integrase-expressing transgene (BestGene Inc.). The orientation of the FlpStop cassette was identified using primers MiL-F ...
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bioRxiv - Microbiology 2024Quote: ... The resulting pUASt-based plasmids containing the VSV G or GP64 fragments were injected into W1118 flies (BestGene Inc, California), and transgenic fly lines were verified for expression of the viral envelope proteins (WT and ΔY versions of VSV G and GP64 ...
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bioRxiv - Developmental Biology 2021Quote: ... Resultant plasmid was verified by sequencing and transgenic flies were generated using strain attP2 by PhiC31 integrase-mediated transgenesis (BestGene Inc.).
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bioRxiv - Cell Biology 2021Quote: ... the pGE-attB-GMR integration plasmid containing the new allele was injected into αTub84BattP-KO embryos expressing the integrase PhiC31 (BestGene Inc.). The following mutations were introduced into the integration plasmid by Phusion high fidelity polymerase ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... UAS-HA-YTHDF and UAS-HA-YTHDF-3A (described above) were injected into w[1118] with Δ2-3 helper plasmid to obtain transformants (Bestgene, Inc.) Flies were raised on standard cornmeal-based food medium containing 1.25% w/v agar ...
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bioRxiv - Developmental Biology 2022Quote: ... The donor vector and gRNA plasmid (pCFD5-4) were co-injected into vas-Cas9 embryos (BL-55821; BestGene Inc, Chino Hills, CA) and the single RFP+ line obtained was balanced ...
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bioRxiv - Neuroscience 2023Quote: ... attB-containing plasmids with ppk1 knock-in alleles were injected into ppk1attP-KO embryos expressing ΦC31 integrase (BestGene Inc., Chino Hills, CA). The GMR-mini-w+ and 3xP3-DsRed markers were subsequently removed by crossing to flies expressing Cre recombinase.
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bioRxiv - Developmental Biology 2022Quote: ... The obtained tey-pCFD6-4 plasmid containing the four tey guide RNAs under the control of UAS sequences were injected for insertion into AttB40 (BestGene Inc, Chino Hills, CA). Homozygous transgenic lines were crossed with nos-Gal4VP14 UAS-cas9 and balanced flies from established lines were tested by PCR and sequencing for CRISPR/Cas9-induced deletions in tey.
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bioRxiv - Developmental Biology 2021Quote: ... The gRNA plasmid was confirmed by sequencing and injected into transgenic embryos expressing Vasa-Cas9 and allowed to develop to adulthood (BestGene Inc., Chino Hills, USA). Each adult fly was individually crossed with a balancer stock to generate stocks of putative mutants ...
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bioRxiv - Neuroscience 2023Quote: Transgenic flies harboring the FlpStop 2.0 cassette in an intron of the Ecdysone Receptor (EcR) were generated via standard construct injection (∼50ng plasmid) by Bestgene (Chino Hills, CA, USA). The MiMIC strain152 used for injection was y[1] w[*] ...