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Citations for Akoya Biosciences :
101 - 140 of 140 citations for WD Repeat Containing Planar Cell Polarity Effector WDPCP Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated with spectral DAPI (Akoya Biosciences, cat #SKU FP1490), for 5 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... All images were analyzed using inForm Cell Analysis software (Akoya Biosciences). Sixty-three images of encompassing the 26 different control and experimental slides were batched analyzed after using a mouse formulated library consisting of each single TSA fluorophore listed above ...
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bioRxiv - Microbiology 2021Quote: Antigen retrieval and blocking were performed on the dewaxed slides using the Antigen retrieval reagent (pH 6.0) and Blocking/antibody diluent provided in the Opal Polaris Multicolor Manual IHC Detection Kit (Akoya Biosciences, USA), following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... polymer-conjugated secondary antibody for 20 min and reacted with one of the tyramide signal amplification reagents including Opal 520 (Akoya, FP1487001KT), Opal 570 (Akoya ...
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bioRxiv - Cancer Biology 2021Quote: ... was used for antibody raised in rabbit (PH3 and PHGDH) and the OPAL Polymer HRP Ms+Rb (Akoya/Perkin Elmer, ARH1001EA) was used for the antibody raised in mouse (panCK) ...
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bioRxiv - Cancer Biology 2023Quote: ... Epitope retrieval/microwave treatment (MWT) for all antibodies was performed by boiling slides in Antigen Retrieval buffer 6 (AR6 pH6; Akoya, AR6001KT). Protein blocking was performed using antibody diluent/blocking buffer (Akoya ...
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bioRxiv - Developmental Biology 2023Quote: All fixed frozen tissue CODEX antibody stainings were done according to Akoya Biosciences staining protocol associated with the CODEX Staining Kit with some modifications (Akoya; 7000008). After tissue hydration ...
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bioRxiv - Cell Biology 2023Quote: ... Experimental sections (4-μm thickness) and positive tissues were stained with specific primary antibodies (1:100) followed by Opal multiplex immunostaining system (Akoya Biosciences) to generate the stained slides ...
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bioRxiv - Molecular Biology 2024Quote: ... Experimental sections (4-μm thickness) and positive tissues were stained with specific primary antibodies followed by Opal multiplex immunostaining system (Akoya Biosciences) to generate the stained slides ...
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bioRxiv - Cancer Biology 2024Quote: mIHC staining was performed according to the manufacturer’s instructions (OpalTM 4-Color Anti-Rabbit Automation IHC; OpalTM Polymer Anti-Rabbit HRP Secondary Antibody Kit; Akoya Biosciences®). The following primary rabbit antibodies were used for staining ...
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bioRxiv - Immunology 2022Quote: ... secondary HRP-labeled antibody and detection with optimized fluorescent Opal tyramide signal amplification (TSA) dye (Opal 7-color Automation IHC kit, from Akoya, Ref. NEL821001KT) and repeated antibody denaturation cycles ...
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bioRxiv - Developmental Biology 2023Quote: ... The samples were then washed with Tris-NaCl-Tween Buffer after incubation with anti-DIG-POD antibodies and incubated with Cy3-tyramide solution (TSA Plus Cyanine 3; AKOYA Biosciences, NEL744001KT) for 10 min ...
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bioRxiv - Cancer Biology 2023Quote: ... images were analyzed using inForm® Cell Analysis™ software (Akoya Biosciences).
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bioRxiv - Developmental Biology 2023Quote: A manual cell classification was performed in CODEX MAV 1.5 (Akoya Biosciences). Annotation of the macrophage clusters was done using the same gating strategy as in flow cytometry ...
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bioRxiv - Immunology 2022Quote: ... the HRP-coupled secondary anti-rat OmniMap antibody (Ventana Medical Systems) for 12 min and finally Opal 520 fluorophore (Akoya Biosciences, 1:100) at RT for 8 min ...
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Single-cell analysis of shared signatures and transcriptional diversity during zebrafish developmentbioRxiv - Developmental Biology 2023Quote: ... the antibody solution was washed off using PBST and the antibody was developed in dark for 45 minutes without agitation using a tyramide staining solution (TSA PLUS Fluorescein Reagent, Akoya Biosciences, TS-000200) diluted 1:50 in amplification buffer (1X Plus Amplification Diluent ...
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bioRxiv - Cancer Biology 2020Quote: ... Spectral unmixing and cell phenotype identification was performed using inForm software (Akoya Biosciences). Digital immune phenotype plots were created by assembling xy data from identified cell phenotypes in individual tile scans into a whole biopsy composite using ggplot ...
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bioRxiv - Immunology 2021Quote: ... Cells were quantified in a blinded manner using the inForm Software (Akoya Biosciences).
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Cell Ecosystem and Signaling Pathways of Primary and Metastatic Pediatric Posterior Fossa EpendymomabioRxiv - Cancer Biology 2020Quote: ... The LGR5 probe (311021, Advanced Cell Diagnostics) was detected using Opal 520 (FP1487001KT, Akoya Biosciences) at a 1:1500 dilution ...
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bioRxiv - Cancer Biology 2021Quote: Supervised machine learning algorithms were applied for tissue and cell segmentation (inForm 2.4.1, Akoya Biosciences). Single-cell-level imaging data were exported and further processed and analyzed using R (v3.6.2) ...
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bioRxiv - Developmental Biology 2021Quote: ... with probe for human TINAGL1(Advanced Cell Diagnostics #857221-C2) and OPAL 570 fluorophore (Akoya #FP1488001KT), following the manufacturer’s protocol.
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bioRxiv - Cell Biology 2021Quote: ... Mouse (Cat. No. 468281; Advanced Cell Diagnostics) and Opal 690 Dye (Cat. No. FP1497001KT; Akoya BioSciences) were used according to the manufacturer’s instructions for fluorescence in-situ hybridization ...
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bioRxiv - Cancer Biology 2023Quote: Cell numbers of myeloid subsets were estimated with object module of InForm Tissue Analysis Software (AKOYA). Myeloid response score was determined as described previously65,108 ...
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bioRxiv - Immunology 2022Quote: ... Cell classification to detect alveolar macrophages and other mononuclear phagocytes was performed in CODEX MAV (Akoya Biosciences), following a similar gating scheme as the one used for flow cytometry.
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bioRxiv - Cancer Biology 2022Quote: ... anti-ER (Cell Marque, 249-R-15-ASR, clone: SP1, 1:70 dilution) with Opal 650 (Akoya Biosciences ...
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bioRxiv - Cell Biology 2024Quote: ... The ER was then visualized incubating the cells using fluorescent tyramide (TSA-FITC 1:200, AKOYA Biosciences), 0.003 H202 and 0,05% Tween-20 in 100 mM borate buffer ...
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bioRxiv - Immunology 2021Quote: ... the RNAScope multiplex fluorescent kit V2 (Advanced Cell Diagnostics) was used with the opal fluorophores system (Akoya Bioscience) according to the manufacturer’s instructions and as described previously66 with some modifications ...
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bioRxiv - Microbiology 2023Quote: ... and analyzed for several immune cell populations using the inForm® tissue Finder (Version 2.4.8) and PhenochartTM (Version 1.0.12) software (Akoya Biosciences).
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bioRxiv - Cancer Biology 2023Quote: ... The slide was then transferred to the flow cell carrier and imaged on a PhenoCycler-Fusion (Akoya Biosciences, MA) using the following exposure settings ...
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bioRxiv - Cancer Biology 2023Quote: A T cell panel comprising five markers for five channels was generated for imaging with a Vectra (Akoya Biosciences) as previously described41 for validation purposes ...
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bioRxiv - Immunology 2023Quote: ... The vRNA distribution in relation to T cell and B cell zones was conducted with Voronoi spatial analysis (min:3 µm max:30 µm) using CODEX Multiplex Analysis program (Akoya).
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bioRxiv - Neuroscience 2023Quote: ... CasL/NEDD9 (ACD, 446311), and Sin/EFS (ACD, 806951) probes generated by Advanced Cell Diagnostics were uniquely amplified with Opal dyes (Akoya Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: ... Spectral unmixing and further tissue /cell segmentation as well as phenotyping were performed using the inForm Advanced Image Analysis software (inForm v2.4.10, Akoya Biosciences®). Multiplex data analyses ...
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bioRxiv - Neuroscience 2021Quote: In situ hybridization was performed with the RNAscope® Multiplex Fluorescent Assay v2 from Advanced Cell Diagnostics (ACD) combined with Tyramide Signal Amplification technology (TSA™) and dyes from Akoya Biosciences ...
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bioRxiv - Cancer Biology 2020Quote: ... based on canonical marker expression values and were confirmed via visual overlay of the cell populations on the immunofluorescent images utilizing the multiplex analysis viewer (MAV, Akoya Biosciences). Voronoi diagrams were created based on the spatial coordinates of the cell types and utilized to mathematically compute adjacent “cell-cell” contacts ...
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bioRxiv - Immunology 2023Quote: ... Slides were acquired on an automated slide scanning brightfield microscope (Vectra) and positive cells were quantified using inForm automated image analysis software (Akoya Biosciences).
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bioRxiv - Cancer Biology 2023Quote: ... A training set of 9 representative images was used to train analyses algorithms for tissue and cell segmentation and phenotyping using inForm software (Akoya Biosciences). Representative autofluorescence was measured on an unstained control slide and subtracted from study slides ...
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bioRxiv - Systems Biology 2023Quote: ... RNA in situ detection was performed using the RNAscope Multiplex Fluorescent V2 Assay kit (Advanced Cell Diagnostics) in combination with OPAL dyes 520 and 570 (Akoya Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The green and red dyes were prepared at a concentration of 1:1000 by combining 1000 µl of TSA Buffer (Advanced Cell Diagnostics, 322809) with 1 µl of Opal 520 Reagent (in DMSO, Akoya Biosciences, OP-001001) and Opal 570 Reagent (in DMSO ...
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bioRxiv - Neuroscience 2024Quote: ... Kcnv1 mRNA was detected using the target probe Mm-Kcnv1 (Advanced Cell Diagnostics) and the Opal™ 650 dye (1:1500, Akoya Biosciences, Marlborough, MA, USA)