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Citations for Akoya Biosciences :
101 - 150 of 208 citations for Roundabout Guidance Receptor 1 ROBO1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Slides were then blocked with 10% BSA + 0.05% Tween20 and the antibodies were diluted in 2% BSA in 0.05% PBST and used in a multiplexed manner with OPAL reagents (Akoya Biosciences). All primary antibodies (described above for western blotting ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were then blocked with 10% BSA + 0.05% Tween20 and the antibodies were diluted in 2% BSA in 0.05% PBST and used in a multiplexed manner with OPAL reagents (Akoya Biosciences). All primary antibodies were incubated overnight at 4 °C ...
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bioRxiv - Pathology 2024Quote: ... we stained antibodies on tonsil tissue and aorta tissue microarray using the sample kit for PhenoCycler-Fusion (Akoya Biosciences, 7000017) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... All other antibodies were custom conjugated to their respective CODEX barcode (Supplemental Table S5) according to Akoya’s PhenoCycler-Fusion user guide using the antibody conjugation kit (Akoya, 7000009) following manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... and 1/1000 for TSA-CY5 (Akoya Biosciences, NEL745001KT). To reveal C1 probes ...
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bioRxiv - Microbiology 2022Quote: ... Opal 570 Reagent Pack (1:1500; Akoya Biosciences, FP1488001KT) for detection of HDV RNA ...
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bioRxiv - Neuroscience 2023Quote: ... Detection of mRNA was achieved using Opal™ fluorophore dyes from the 520 (1:500) and 620 (1:750) reagent packs (Akoya Biosciences, USA). Full protocol details are available in Supplementary Methods ...
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bioRxiv - Microbiology 2021Quote: Antigen retrieval and blocking were performed on the dewaxed slides using the Antigen retrieval reagent (pH 6.0) and Blocking/antibody diluent provided in the Opal Polaris Multicolor Manual IHC Detection Kit (Akoya Biosciences, USA), following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... polymer-conjugated secondary antibody for 20 min and reacted with one of the tyramide signal amplification reagents including Opal 520 (Akoya, FP1487001KT), Opal 570 (Akoya ...
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bioRxiv - Cancer Biology 2022Quote: ... The tissue was washed and equilibrated before staining for 3 hours at room temperature with the 28-plex CODEX antibody cocktail in a staining buffer containing blocking solution (Akoya Biosciences). After staining ...
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bioRxiv - Cancer Biology 2021Quote: ... was used for antibody raised in rabbit (PH3 and PHGDH) and the OPAL Polymer HRP Ms+Rb (Akoya/Perkin Elmer, ARH1001EA) was used for the antibody raised in mouse (panCK) ...
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bioRxiv - Cancer Biology 2023Quote: ... Epitope retrieval/microwave treatment (MWT) for all antibodies was performed by boiling slides in Antigen Retrieval buffer 6 (AR6 pH6; Akoya, AR6001KT). Protein blocking was performed using antibody diluent/blocking buffer (Akoya ...
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bioRxiv - Developmental Biology 2023Quote: All fixed frozen tissue CODEX antibody stainings were done according to Akoya Biosciences staining protocol associated with the CODEX Staining Kit with some modifications (Akoya; 7000008). After tissue hydration ...
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bioRxiv - Molecular Biology 2024Quote: ... Experimental sections (4-μm thickness) and positive tissues were stained with specific primary antibodies followed by Opal multiplex immunostaining system (Akoya Biosciences) to generate the stained slides ...
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bioRxiv - Cancer Biology 2024Quote: mIHC staining was performed according to the manufacturer’s instructions (OpalTM 4-Color Anti-Rabbit Automation IHC; OpalTM Polymer Anti-Rabbit HRP Secondary Antibody Kit; Akoya Biosciences®). The following primary rabbit antibodies were used for staining ...
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bioRxiv - Cell Biology 2023Quote: ... and 1:2000 TSA Plus Cy5 for C2-Pdgfrβ (Akoya, FP1487001 ...
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bioRxiv - Developmental Biology 2023Quote: ... and Opal-570 reagent pack (Akoya Biosciences, #FP1488001KT, 1:2000) for the C3 probe ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-Cytokeratin 5 (1:300 dilution) with Opal 690 (Akoya Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1:750 dilution of TSA-Cyanine 3 Plus (AKOYA). The RNAscope® 3-plex LS Multiplex Negative Control Probe (Bacillus subtilis dihydrodipicolinate reductase (dapB ...
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bioRxiv - Neuroscience 2024Quote: ... and 620 fluorescent dyes (1:5000, Akoya Biosystems, FP1487001KT, FP1495001KT). All HRP solutions (C1-C2 ...
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bioRxiv - Immunology 2022Quote: ... secondary HRP-labeled antibody and detection with optimized fluorescent Opal tyramide signal amplification (TSA) dye (Opal 7-color Automation IHC kit, from Akoya, Ref. NEL821001KT) and repeated antibody denaturation cycles ...
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bioRxiv - Developmental Biology 2023Quote: ... The samples were then washed with Tris-NaCl-Tween Buffer after incubation with anti-DIG-POD antibodies and incubated with Cy3-tyramide solution (TSA Plus Cyanine 3; AKOYA Biosciences, NEL744001KT) for 10 min ...
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bioRxiv - Molecular Biology 2023Quote: ... and 690 fluorophores were used to visualize expression (1:1,000; Akoya Biosciences ...
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bioRxiv - Physiology 2022Quote: ... followed by Opal 690 (1:500, Akoya Biosciences, Cat. No. FP1497001KT). Negative controls for each immunostaining were incubated with secondary antibody only.
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bioRxiv - Neuroscience 2023Quote: ... for C1 and Opal 570 Reagent (1:750; Akoya Biosciences, #FP1488001KT) for C3 ...
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bioRxiv - Developmental Biology 2023Quote: ... we used Opal-690 reagent pack (Akoya Biosciences, FP1497001KT, 1:750) for the C1 probe ...
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bioRxiv - Immunology 2023Quote: ... Opal 570 was used for signal amplification (1:100, Akoya Bioscience). Counterstaining was performed with DAPI for 5 min at room temperature (1 drop per 500 µL ...
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Single-cell analysis of shared signatures and transcriptional diversity during zebrafish developmentbioRxiv - Developmental Biology 2023Quote: ... the antibody solution was washed off using PBST and the antibody was developed in dark for 45 minutes without agitation using a tyramide staining solution (TSA PLUS Fluorescein Reagent, Akoya Biosciences, TS-000200) diluted 1:50 in amplification buffer (1X Plus Amplification Diluent ...
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bioRxiv - Neuroscience 2021Quote: ... Opal 570 dye (Akoya Biosciences; 1:1500; 30 min at 40°C), followed by HRP blocker (15 min at 40°C) ...
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bioRxiv - Neuroscience 2022Quote: ... Probes were used at 1:50 dilution and Opal dyes from Akoya Biosciences were used at 1:100 dilution ...
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bioRxiv - Cell Biology 2022Quote: ... and 1:750 dilution of TSA-Cyanine 5 Plus (AKOYA, SKU NEL745001KT) and TSA-Cyanine 3 Plus (AKOYA ...
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bioRxiv - Neuroscience 2023Quote: ... slices were incubated in tyramide conjugated fluorescein (Akoya Biosciences, NEL741001KT, 1:50) for 10 min ...
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bioRxiv - Cancer Biology 2024Quote: ... diluted 1:300 in 1X Plus Automation Amplification Diluent (Akoya Biosciences, #FP1609). All steps were carried out at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... TSA reagents were diluted with 1×□Plus Amplification Diluent (PerkinElmer/Akoya Biosciences, US).
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bioRxiv - Immunology 2022Quote: ... with OmniMap anti-rabbit-HRP and Opal 690 fluorophore (Akoya Biosciences, 1:50). Finally ...
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bioRxiv - Immunology 2022Quote: ... with OmniMap anti-rabbit-HRP and Opal 570 fluorophore (Akoya Biosciences, 1:1000), and lastly CD3 (abcam ...
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bioRxiv - Microbiology 2022Quote: ... and 1:750 dilution of TSA Plus-Cyanine 3 (AKOYA Biosciences, cat# NEL744001KT) and TSA Plus-Cyanine 5 (AKOYA Biosciences ...
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Single-cell analysis of shared signatures and transcriptional diversity during zebrafish developmentbioRxiv - Developmental Biology 2023Quote: ... diluted 1:50 in amplification buffer (1X Plus Amplification Diluent, Akoya Biosciences, FP1135). Next ...
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bioRxiv - Neuroscience 2023Quote: ... The vGLUT2 immunoreaction was enhanced with tyramide signal amplification (1:50; Akoya Biosciences). Next ...
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bioRxiv - Immunology 2024Quote: ... Slides were then incubated with TSA Plus (Opal 570, 1:100, Akoya Biosciences) for 10 minutes at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... The C4 probe complexes were first incubated with TSA–Biotin (Akoya NEL700A001KT, 1:250) for 30 min at RT ...
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bioRxiv - Developmental Biology 2022Quote: ... The C4 probe complexes were first incubated with TSA–Biotin (Akoya NEL700A001KT, 1:250) for 30 min at RT ...
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bioRxiv - Cancer Biology 2021Quote: ... for 12 minutes and with Opal 780 reactive fluorophore for 1 hour (Akoya Biosciences). Before each round of staining ...
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bioRxiv - Neuroscience 2021Quote: ... fos mRNA was then tagged to the fluorophore fluorescein (1:1500; NEL741E001KT, Akoya Biosciences), and v1aR-mRNA was tagged to the fluorophore Cy5 (1:1500 ...
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bioRxiv - Neuroscience 2021Quote: ... and v1aR-mRNA was tagged to the fluorophore Cy5 (1:1500; NEL745E001KT, Akoya Biosciences). Slides were then rinsed in TBS and stained with a fluorescent Nissl (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were treated with TSA-plus Cyanine 3 (NEL744001KT; Akoya Biosciences; 1:1500). Fluoromount (K024 ...
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bioRxiv - Neuroscience 2023Quote: ... Cy3 or fluorescein at 1:1000 in TSA buffer (TSATM Plus System, Akoya Bioosciences). Following that slides were incubated in HRP blocker for 15 min and then rinsed 3 times with wash buffer reagent ...
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bioRxiv - Cancer Biology 2023Quote: ... the slides were developed with Opal fluorescent dye at 1:50 dilution (Akoya Biosciences) in different channels ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by incubation in Opal 690 reagent for 15 minutes (Akoya Biosciences, 1:100) in blocking buffer ...
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bioRxiv - Neuroscience 2023Quote: ... HRP-C2 and HRP-C3 were labelled with Opal 520 (1:750, Akoya FP1487001KT) and Opal 570 (1:1500 ...