Labshake search
Citations for Akoya Biosciences :
1 - 50 of 148 citations for Retinol Binding Protein RBP Multi format ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2024Quote: The multi-IF was performed using the Opal 6-Plex Manual Detection Kit (NEL811001KT, Akoya), following the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2023Quote: ... The unmixing algorithm was applied to the annotated region to decompose multi-spectral profiles into spectrally unmixed multi-channel TIFF tiles using inForm automated image analysis software (Akoya Biosciences). Autofluorescence ...
-
bioRxiv - Developmental Biology 2021Quote: ... TSA Plus Cyanine 5 Kit (Akoya Biosciences, NEL745001KT) was used ...
-
bioRxiv - Cancer Biology 2020Quote: Multi-spectral IHC analyses were performed using Vectra Automated Quantitative Pathology Systems (Akoya Biosciences) as previously described [25] ...
-
bioRxiv - Pathology 2023Quote: ... 20x high power fields were selected to image all dense lymphocytic aggregates multi-spectrally in each slide and images were processed using the Inform software v2.6 (Akoya).
-
bioRxiv - Molecular Biology 2021Quote: ... The binding sites of HRP-conjugated secondary antibodies were detected with Red Opal (1:100, AKOYA Biosciences) and a DAPI counterstain was applied (1:1000) ...
-
bioRxiv - Immunology 2023Quote: ... TIFFs from single fields of view were then stitched together fused into a single multi-layer pyramidal TIFF in Halo software (Indica Labs) that were unmixed using inForm software (Akoya Biosciences) utilizing an algorithm based on a library of fluorescence spectra generated from control slides labeled with single fluorophores ...
-
bioRxiv - Cell Biology 2022Quote: ... The binding of antibodies were detected with a 1:150 dilution of OPAL 520 Reagent (AKOYA, SKU FP1487001KT). Slides were digitally imaged using an Aperio ScanScope FL Scanner (Leica Biosystems).
-
bioRxiv - Cancer Biology 2023Quote: ... incubation times and appropriate antibody concentrations were optimized using the Opal™ 5-color kit (Akoya Biosciences, NEL840001KT). Epitope stability was assessed to determine the order of each antibody in sequence ...
-
bioRxiv - Immunology 2023Quote: ... Antibody binding was visualized using secondary anti-mouse/anti-rabbit polymer HRP followed with TSA-Opal reagents (Akoya NEL811001KT). Tissue slides were incubated with DAPI as a counterstain and coverslipped with VectaShield mounting media (Vector Labs) ...
-
bioRxiv - Immunology 2020Quote: We stained 5-µm-thick formalin-fixed paraffin-embedded sections from human xenograft tumors (UMSCC-1) using Opal multiplex kits (PerkinElmer/Akoya), for a panel of DAPI ...
-
bioRxiv - Developmental Biology 2023Quote: ... followed by streptavidin-conjugated peroxidase incubation and signal amplification using the TSA Plus Cyanine-3 or -5 kits (Akoya Biosciences).
-
bioRxiv - Neuroscience 2021Quote: ... Slides were then washed for 3 x 5 minutes in NTT and developed with Cy5-Tyramide signal amplification reagent (TSA Plus Kit, Akoya Biosciences #NEL745001KT) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2024Quote: ... Cyanine 3 and Cyanine 5 reagents from Akoya Biosciences were used for probe visualization.
-
bioRxiv - Neuroscience 2024Quote: ... Cyanine 3 and Cyanine 5 reagents from Akoya Biosciences at 1:1500 ...
-
bioRxiv - Cancer Biology 2024Quote: ... a 5 min DAPI staining (Akoya Biosciences, #FP1490) was performed before mounting with ProLong Diamond Antifade media (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2021Quote: ... in light-protected 96-well plates sealed with foil (Akoya Biosciences) and automated image acquisition and fluidics exchange were performed using the Akoya CODEX instrument and CODEX Instrument Manager (CIM ...
-
bioRxiv - Developmental Biology 2024Quote: ... embryos were washed for 2 days at 4◦C and then incubated for 5 minutes with TSA Cyanine 5 at room temperature (NEL705A001, Akoya, biosciences). All embryos were nuclei stained with DAPI 1:1000 diluted in PBS ...
-
bioRxiv - Microbiology 2022Quote: ... and TSA Plus-Cyanine 5 (AKOYA Biosciences, cat# NEL745001KT), respectively ...
-
bioRxiv - Cancer Biology 2022Quote: Stained coverslips were mounted onto the CODEX stage plate version 2 (Akoya) and secured onto the stage of a BZ-X810 inverted fluorescence microscope (Keyence) ...
-
bioRxiv - Cancer Biology 2022Quote: ... anti-Cytokeratin 5 (1:300 dilution) with Opal 690 (Akoya Biosciences ...
-
bioRxiv - Neuroscience 2023Quote: ... signals were amplified with TSA-plus Cyanine 5 (NEL745001KT, Akoya Biosciences ...
-
bioRxiv - Immunology 2021Quote: ... protein blocking in Akoya Antibody Diluent/Block (Akoya biosciences, MA) took place for 10 minutes ...
-
bioRxiv - Cancer Biology 2023Quote: ... Protein blocking was performed using antibody diluent/blocking buffer (Akoya, ARD1001EA) for 10 minutes at room temperature ...
-
bioRxiv - Cell Biology 2022Quote: ... and 1:750 dilution of TSA-Cyanine 5 Plus (AKOYA, SKU NEL745001KT) and TSA-Cyanine 3 Plus (AKOYA ...
-
bioRxiv - Developmental Biology 2023Quote: ... Bound probes were detected using TSA Plus fluorophores Cyanine 5 (Akoya Bioscience, NEL745001KT) and Cyanine 3 (Akoya Bioscience ...
-
bioRxiv - Developmental Biology 2024Quote: ... Fluorescence was revealed with the TSA+ Cyanin 3 or 5 system (AKOYA biosciences).
-
bioRxiv - Cancer Biology 2023Quote: Opal Multiplex IF Kit (Akoya) was used to perform multiplex immunofluorescence according to manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2023Quote: ... Signal amplification was done with 1:50 TSA Plus Cyanine-3 or -5 (Akoya Biosciences) for 1 h at RT ...
-
bioRxiv - Pathology 2019Quote: ... 7-color manual IHC kit (Akoya Biosciences ...
-
bioRxiv - Neuroscience 2023Quote: ... TSA-Cy3 Plus kit (Akoya #NEL744001KT) was used to detect anti-DIG antibody at 1:200 ...
-
bioRxiv - Neuroscience 2022Quote: ... washed again (2 x 5 min) and incubated with OPAL 650 Tyramide (Akoya Biosciences, FP1496001KT, 1:500) for 10 minutes ...
-
bioRxiv - Cell Biology 2023Quote: ... or the tyramide signal amplification (TSA) fluorophores (TSA Fluorescein, TSA Cyanine 3, TSA Cyanine 5) (Akoya Biosciences) were used for detection ...
-
bioRxiv - Immunology 2022Quote: ... was stained with 6 protein markers (Figure 4A) and captured with PhenoImager® imaging system (Akoya Biosciences). Over 15,000 cell type annotations from 35 training set slides were used as a training set ...
-
bioRxiv - Systems Biology 2023Quote: ... Proximal protein biotinylation occurs with treatment of H2O2 and tyramide-biotin (TSA Biotin System, Akoya Bioscience NEL700A001KT), resulting in tyramide-biotin radicalization and covalent deposition onto proximal proteins ...
-
bioRxiv - Pathology 2022Quote: ... using CODEX conjugation kit (Cat#: 7000009, Akoya) following CODEX conjugation manual.
-
bioRxiv - Neuroscience 2021Quote: ... 570 and 690 reagent kits (Akoya Biosciences). Cells were stained with DAPI (ACD ...
-
bioRxiv - Developmental Biology 2023Quote: ... using TSA Plus amplification kits (Akoya Biosciences) and mouse anti-β-galactosidase (Promega #Z378 ...
-
bioRxiv - Neuroscience 2023Quote: ... tissue was washed before ISH signal was developed with the TSA Plus Cyanine 5 System (Akoya Biosciences # NEL745001KT). Slides were then stripped of horseradish peroxidase and blocked with normal goat serum before incubating with goat anti-rabbit Alexa Fluor 488 (1:400 ...
-
bioRxiv - Immunology 2023Quote: ... Counterstaining was performed with DAPI for 5 min at room temperature (1 drop per 500 µL, Akoya Bioscience). Microscope images were taken using the Zeiss AxioImager M2 and analyzed using ZEN blue software (v2.3).
-
bioRxiv - Neuroscience 2022Quote: ... CODEX reporter 96-well plates were prepared using the cycle reporter configuration as outlined in the CODEX user manual (Akoya Biosciences, 2021a). Imaging was performed using a Keyence BZ-X800 epifluorescence microscope equipped with either a 20x objective (Nikon CFI Plan Apo 20x/0.75 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Additional antibodies were obtained from commercial vendors as indicated and conjugated to custom oligo barcodes according to instructions in Akoya’s antibody conjugation kit (Conjugation kit, Akoya, #7000009). Briefly ...
-
bioRxiv - Immunology 2023Quote: ... and 4-Plex Ancillary Kit (ACD Biotechne, Cat. No. 323120) kits were used in combination with Opal reagents from Akoya Biosciences (Opal 520 – Cat ...
-
bioRxiv - Developmental Biology 2021Quote: ... using the TSA Plus fluorescein detection kit (Akoya Biosciences ...
-
bioRxiv - Cancer Biology 2022Quote: ... or TSA Plus Fluorescein kit (Akoya Biosciences NEL741001KT) were used to amplify signal for immunohistochemistry or immunofluorescence ...
-
bioRxiv - Cancer Biology 2022Quote: ... using OPAL detection kits (ref NEL871001KT, AKOYA bioscience). Primary antibodies specific to Survivin (1/400 ...
-
bioRxiv - Neuroscience 2020Quote: ... Fluorescence signals were amplified and visualized using the TSA Plus Cyanine-5 and the TSA Plus Fluorescein systems (Akoya Biosciences ...
-
bioRxiv - Cancer Biology 2021Quote: ... 1:200 in TNB blocking buffer) for 45 min followed by DAPI staining for 5 min (Spectral DAPI, Akoya Biosciences ...
-
bioRxiv - Immunology 2022Quote: ... 5 ROIs in 34 WSI from the melanoma dataset were spectrally unmixed using inForm® software v6.4.2 (Akoya Biosciences), and analyzed with the HALO® image analysis platform (Indica Labs) ...
-
bioRxiv - Cancer Biology 2022Quote: ... tissue slides were exposed to multiple staining cycles each including a 30 minute incubation with a protein block (Akoya), 1 hour incubation with the respective primary antibody ...