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Citations for Akoya Biosciences :
51 - 100 of 120 citations for Rabbit Anti Borrelia burgdorferi sensu stricto B31 CRASP 2 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: Commercially available oligonucleotide conjugated antibodies were purchased from Akoya Biosciences and used at a standard dilution of 1/200 (Supplemental Table 1) ...
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bioRxiv - Cancer Biology 2023Quote: Commercially available oligo-conjugated antibodies were obtained from Akoya Biosciences (Marlborough ...
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bioRxiv - Developmental Biology 2024Quote: ... 100 μl of antibody storage buffer (Akoya Biosciences, 7000009) was added on top of antibodies and centrifuged (3000 x g ...
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bioRxiv - Cancer Biology 2021Quote: ... the slides were counterstained with 4’,6-diamidino-2-phenylindole (DAPI, Akoya Biosciences) and coverslipped using ProLong Gold antifade mounting media (Thermo Fisher Scientific) ...
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bioRxiv - Pathology 2021Quote: IHC tissues were imaged with a Mantra 2 Quantitative Pathology Workstation (Akoya Biosciences) with a 20x objective ...
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bioRxiv - Cancer Biology 2023Quote: ... 2x for 2 min and incubated in CODEX staining buffer (Akoya Biosciences, 232106) for 20 min ...
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bioRxiv - Immunology 2023Quote: ... CDKN1A (channel 2; ACD 311401-C2) conjugated to Opal Polaris 780 (Akoya FP1501001KT), and IL1B (channel 4 ...
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bioRxiv - Neuroscience 2023Quote: ... assay # 2: VIP expression was identified by Opal 520 (Akoya Bioscience, catalog # FP1487001KT), CCK expression was identified by Opal 570 (Akoya Bioscience ...
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bioRxiv - Cancer Biology 2021Quote: Tissue sections were baked for 3 hours at 62°C in vertical slide orientation with subsequent deparaffinization performed on the Leica Bond RX followed by 30 minutes of antigen retrieval with Leica Bond ER2 followed by 6 sequential cycles of staining with each round including a 30-minute combined block and primary antibody incubation (Akoya antibody diluent/block ARD1001).
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bioRxiv - Cancer Biology 2023Quote: 4 µm FFPE tissue sections were baked for 2.5 hours at 63°C in vertical slide orientation with subsequent deparaffinization performed on the Leica Bond RX followed by 30 min of antigen retrieval with Leica Bond ER2 followed by 6 sequential cycles of staining with each round including a 30-minute combined block and primary antibody incubation (Akoya antibody diluent/block ARD1001EA) except HER2 ...
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bioRxiv - Immunology 2024Quote: ... Biotinylated antibodies were visualized with Streptavadin-AF594 (Cat. No.: S32356) and the CD4 antibody was visualized with OPAL 520 (Akoya Biosciences, Cat. No.: FP1487001KT). The ROI’s selected to undergo GeoMx® DSP spatial transcriptomics were chosen based on Lyve-1 staining ...
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bioRxiv - Immunology 2023Quote: ... The nuclei were stained with spectral DAPI (4,6-diamidino-2-phenylindole) solution (Akoya Biosciences). Finally ...
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bioRxiv - Physiology 2022Quote: ... antibodies were reduced using a “Reduction Master Mix” (Akoya Biosciences) to which lyophilized barcodes resuspended in molecular biology grade water and “Conjugation Solution” (Akoya Biosciences ...
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bioRxiv - Physiology 2022Quote: ... Antibodies were imaged cyclically using the CODEX system from Akoya Biosciences and a Keyence BZ-X810 slide scanning microscope fitted with a 20x air objective (0.75 NA) ...
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bioRxiv - Immunology 2021Quote: ... protein blocking in Akoya Antibody Diluent/Block (Akoya biosciences, MA) took place for 10 minutes ...
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bioRxiv - Genomics 2023Quote: ... and incubated with the primary antibody before being probed with an appropriate OPAL™ secondary antibody conjugated to a fluorophore (Akoya Biosciences, Supplementary Table 21). This was followed by a further HIER to remove the primary antibody before staining with the next primary/secondary combination ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein blocking was performed using antibody diluent/blocking buffer (Akoya, ARD1001EA) for 10 minutes at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... tissue sections were incubated with 1X Antibody Diluent/Block (Akoya Biosciences) for 5 minutes ...
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bioRxiv - Immunology 2022Quote: ... Primary antibodies were detected with OPAL Polymer HRP Ms + Rb (Akoya Biosciences) for 15 minutes and visualized following a 10-minute incubation with Tyramide signal amplification and Opal Fluorophore (Akoya ...
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bioRxiv - Immunology 2023Quote: ... Primary antibodies were diluted in 1X Plus Automation Amplification Diluent (Akoya Biosciences). Primaries were detected using according anti-species secondary antibodies conjugated to HRP (OmniMap Ventana ...
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bioRxiv - Cancer Biology 2024Quote: ... Scanning of the full antibody panel was performed on PhenoImager (Akoya Biosciences). A spectral fluorophore for each primary antibody and autofluorescence library was made to enable optimal multispectral unmixing ...
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bioRxiv - Cancer Biology 2022Quote: ... The tissue was then allowed to dry for 2 minutes and hydrated in hydration buffer (Akoya Biosciences) twice for 2 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... washed again (2 x 5 min) and incubated with OPAL 650 Tyramide (Akoya Biosciences, FP1496001KT, 1:500) for 10 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... and SARS-CoV-2 spike mRNA with TSA Plus CY5 (1:1500, #NEL745001KT, Akoya Biosciences, MA, USA). Fluorescent images were acquired with NanoString GeoMX DSP at 20×.
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Secondary antibodies were obtained from Akoya (OPAL 690, OPAL 520, and OPAL 570) and diluted to a concentration of 1:750 ...
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bioRxiv - Cancer Biology 2023Quote: ... panCK staining was performed by using an OPAL TSA-DIG antibody (Akoya Biosciences) in combination with an OPAL 780 fluorophore conjugated anti-DIG antibody ...
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bioRxiv - Immunology 2023Quote: ... Mixed RNAscope (Advanced Cell Diagnostics)/antibody antigen retrieval and staining with Opal (Akoya Biosciences) fluorophores was performed manually following the RNAscope Multiplex Fluorescent v2 Assay combined with the immunofluorescence protocol (322818-TN ...
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bioRxiv - Pathology 2024Quote: ... To validate the efficacy of commercial DNA-barcode-conjugated antibodies for PhenoCycler by Akoya Biosciences ...
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bioRxiv - Immunology 2023Quote: ... The tissue section was stained with a cocktail of DNA-barcoded antibodies purchased from Akoya company (CD4/EPR6855 ...
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bioRxiv - Bioengineering 2023Quote: The antibody conjugation and the staining were done according to the manufacturer’s protocol (Akoya Biosciences). Details regarding primary antibody ...
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bioRxiv - Cancer Biology 2024Quote: ... Signal for antibody complexes was labeled and visualized by their corresponding Opal Fluorophore Reagents (Akoya) by incubating the slides for 10 minutes ...
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bioRxiv - Immunology 2022Quote: ... several steps are carried out in order to amplify the IL-22BP signal: polymer enhancer (M023 from ImPath) then HRP-2 (M024 from ImPath) and finally Opal 650 (FP1496A from Akoya) following manufacturer’s instruction ...
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bioRxiv - Pathology 2023Quote: ... Spatial co-localization of CD34 and fibrinogen was carried out using the 2-color IHC images obtained with the Mantra 2.0 microscope (Akoya Biosciences) with spectral unmixing using Inform software ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were blocked with 10% BSA + 0.05% Tween 20 and the antibodies listed in Supplementary Table 4 were diluted in 2% BSA in 0.05% PBST and used in a multiplexed manner using the OPAL reagents (Akoya Biosciences). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-Cytokeratin 5 (1:300 dilution) with Opal 690 (Akoya Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... Opal Anti-Ms + Rb HRP (Akoya Biosciences, Marlborough, MA ARH1001EA), before conjugating with Opal fluorophores Following incubation ...
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bioRxiv - Immunology 2021Quote: ... antibodies was performed using the OPAL Automation Multiplex IHC Detection Kit (Akoya Biosciences, Catalog 160 #NEL830001KT) implemented onto a BOND Research Detection System (DS9455) ...
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bioRxiv - Neuroscience 2023Quote: ... followed by a 10-minute incubation with Opal Antibody Dilutent/Block solution (Akoya Bioscience, Marlborough, USA). The first primary antibody was then applied overnight at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated for 2hr with HRP-conjugated anti-mouse IgG diluted at 1:300 and followed by 1 hr incubation of TSA plus Cyanine-2 (1:300; cat#NEL745001KT, Akoya Biosciences). Sections were counterstained with DAPI and mounted on slides with CC/Mount mounting medium (cat# C9368 ...
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bioRxiv - Bioengineering 2022Quote: ... The fluorescent Opal 570 dye was added to channel 2 containing SLC17A7-C2 (excitation: 550 nm, emission: 570 nm; Akoya Biosciences). DAPI was added in the final step to spheroids for 30-sec ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were washed for 2 days at 4◦C and then incubated for 5 minutes with TSA Cyanine 5 at room temperature (NEL705A001, Akoya, biosciences). All embryos were nuclei stained with DAPI 1:1000 diluted in PBS ...
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bioRxiv - Physiology 2022Quote: 14 of the 23 antibodies used here were conjugated in-house using the protocol outlined by Akoya Biosciences (Table 3)19,20 ...
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bioRxiv - Molecular Biology 2021Quote: ... The binding sites of HRP-conjugated secondary antibodies were detected with Red Opal (1:100, AKOYA Biosciences) and a DAPI counterstain was applied (1:1000) ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were then rinsed through two rounds of incubation in distilled water for 2 minutes and stored in Hydration Buffer from the PhenoCycler Staining Kit (Akoya Biosciences, MA, #7000008) until ready for staining.
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bioRxiv - Immunology 2021Quote: ... RNA probes and panCK antibody were detected with the following Opal Fluorophores pairings: CXCL13 Opal 480 (Akoya FP1500001KT), CXCL10/11 Opal 520 (Akoya FP1487001KT) ...
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bioRxiv - Immunology 2022Quote: ... Each target was linked using the OPAL Polymer HRP conjugated secondary antibodies (ready-to-use, ARH1001EA, AKOYA Biosciences). Visualization of the different targets was established using the OPAL Polaris 570 (FP1488001KT ...
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bioRxiv - Cancer Biology 2023Quote: ... incubation times and appropriate antibody concentrations were optimized using the Opal™ 5-color kit (Akoya Biosciences, NEL840001KT). Epitope stability was assessed to determine the order of each antibody in sequence ...
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bioRxiv - Cancer Biology 2022Quote: ... Slides were washed in TBS + 0.1% tween (TBS-T) and blocked in Antibody Diluent/Block (Akoya Biosciences, ARD1001EA) for 30 minutes at RT ...
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bioRxiv - Cell Biology 2022Quote: ... The binding of antibodies were detected with a 1:150 dilution of OPAL 520 Reagent (AKOYA, SKU FP1487001KT). Slides were digitally imaged using an Aperio ScanScope FL Scanner (Leica Biosystems).
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bioRxiv - Cancer Biology 2021Quote: ... staining was performed at 1:15,000 dilution followed by secondary antibody 570 opal reagent (1:100 dilution) (Akoya Biosciences) and peak wavelength at 555/570 ...