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Citations for Akoya Biosciences :
1 - 50 of 214 citations for Mouse IgG2b Isotype Control Antibody A 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... Negative Control Probe (Akoya biosciences 320871), and Positive Control Probe (Akoya biosciences 320881 ...
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bioRxiv - Developmental Biology 2022Quote: ... and Positive Control Probe (Akoya biosciences 320881) following the kit instructions ...
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bioRxiv - Immunology 2021Quote: ... A mixture of anti-Mouse + anti-Rabbit HRP-conjugated secondary antibodies (Akoya Biosciences) was added for 10 minutes ...
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bioRxiv - Immunology 2022Quote: ... conjugated primary antibodies cocktail (Akoya CD3e 1:100 and Akoya CD20 1:200 in Abcam Antibody Diluent) 2x for 90 minutes each ...
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bioRxiv - Immunology 2023Quote: ... Antibody binding was visualized using secondary anti-mouse/anti-rabbit polymer HRP followed with TSA-Opal reagents (Akoya NEL811001KT). Tissue slides were incubated with DAPI as a counterstain and coverslipped with VectaShield mounting media (Vector Labs) ...
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bioRxiv - Genomics 2024Quote: ... Slides were incubated for 1 hour at room temperature with primary antibodies diluted in 1x Opal blocking/antibody diluent (Akoya biosciences). Slides were subsequently incubated with the Opal Polymer HRP Ms + Rb secondary polymer for 30 minutes prior to incubation with Opal fluorophores (Opal 520 ...
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bioRxiv - Cancer Biology 2021Quote: ... staining was performed at 1:15,000 dilution followed by secondary antibody 570 opal reagent (1:100 dilution) (Akoya Biosciences) and peak wavelength at 555/570 ...
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bioRxiv - Immunology 2021Quote: ... followed by incubation with the first primary antibody for 1 hour, subsequently with the secondary antibody (Polymer HRP, Ms + Rb (Akoya biosciences, MA)) for 30 minutes and finally with an Opal fluorophore ((Akoya biosciences ...
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bioRxiv - Cancer Biology 2021Quote: ... staining was performed at 1:100 dilution followed by secondary antibody 520 reagent (1:100) (Akoya Biosciences; Menlo Park, California) and peak wavelength at 494/525 ...
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bioRxiv - Neuroscience 2024Quote: ... HRP-conjugated anti-flu antibody (at a dilution of 1:250; #NEF710001EA, AKOYA Bioscience) was used to detect Flu-labeled RNA probes by a 1:70 TSA-plus biotin (#NEL749A001KT ...
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bioRxiv - Immunology 2024Quote: ... The images were spectrally unmixed using single stain positive control images in the InForm software (Akoya Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... All antibodies (including pre-conjugated antibodies from Akoya) were additionally validated on the respective GBM control samples using multiple CODEX runs to adjust antibody concentration and exposure times to optimize signal to noise ratio ...
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bioRxiv - Molecular Biology 2021Quote: ... The binding sites of HRP-conjugated secondary antibodies were detected with Red Opal (1:100, AKOYA Biosciences) and a DAPI counterstain was applied (1:1000) ...
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bioRxiv - Cell Biology 2022Quote: ... The binding of antibodies were detected with a 1:150 dilution of OPAL 520 Reagent (AKOYA, SKU FP1487001KT). Slides were digitally imaged using an Aperio ScanScope FL Scanner (Leica Biosystems).
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bioRxiv - Cancer Biology 2023Quote: ... antibodies were conjugated using Akoya Antibody Conjugation Kit (Akoya Biosciences, SKU 7000009) with a barcode (Akoya Biosciences ...
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bioRxiv - Immunology 2021Quote: The antibody panel was composed of commercially available AKOYA-conjugated antibodies and self-conjugated custom antibodies (CODEX Conjugation Kit, AKOYA Biosciences). Tissue staining was performed with the CODEX Staining Kit (AKOYA Biosciences) ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated with HRP-conjugated anti-DIG (1:500) antibody overnight and treated with TSA-plus biotin (NEL749A001KT; Akoya Biosciences), followed by streptavidin-Alexa Fluor 488 (S32354 ...
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bioRxiv - Cell Biology 2023Quote: ... Experimental sections (4-μm thickness) and positive tissues were stained with specific primary antibodies (1:100) followed by Opal multiplex immunostaining system (Akoya Biosciences) to generate the stained slides ...
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bioRxiv - Cancer Biology 2023Quote: ... NRG1 and TGFa primary antibodies were detected with HRP-conjugated antibody (ARR1001KT, Akoya Biosciences) and TSA-based amplification with Opal dye 690 (FP1497001KT ...
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bioRxiv - Immunology 2022Quote: ... conjugated primary antibodies cocktail (Akoya CD3e 1:100 and Akoya CD20 1:200 in Abcam Antibody Diluent ...
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bioRxiv - Molecular Biology 2023Quote: For antibodies purchased from Akoya Biosciences ...
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bioRxiv - Immunology 2022Quote: ... the HRP-coupled secondary anti-rat OmniMap antibody (Ventana Medical Systems) for 12 min and finally Opal 520 fluorophore (Akoya Biosciences, 1:100) at RT for 8 min ...
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bioRxiv - Neuroscience 2021Quote: ... HRP-conjugated anti-flu antibody (AKOYA Bioscience NEF710001EA ...
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bioRxiv - Cell Biology 2021Quote: Antibodies were purchased preconjugated from Akoya Biosciences or sourced from other vendors and conjugated in-house using the CODEX Conjugation Kit (Akoya Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: Antibody conjugation requires reagents from Akoya Biosciences ...
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bioRxiv - Developmental Biology 2024Quote: The high-parametric antibody panel consisted of both a selection of pre-conjugated antibodies (n=23, Akoya Biosciences) and antibodies available through other commercial sources that were conjugated in house (n=49 ...
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bioRxiv - Cell Biology 2022Quote: ... a secondary HRP antibody (ARH1001EA, AKOYA Biosciences) followed by Opal 520 fluorophore (FP1487001KT ...
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bioRxiv - Neuroscience 2022Quote: ... diluted in Antibody Diluent/Block (Akoya, ARD1001EA) for 30min at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... Opal Polymer HRP secondary antibody (AKOYA Biosciences), Opal Fluorophore (AKOYA Biosciences) ...
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bioRxiv - Cancer Biology 2023Quote: ... All antibodies not commercially available from Akoya and hence requiring custom-conjugation with DNA-barcodes were first validated with conventional immunofluorescence (Supplementary Note 2) ...
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bioRxiv - Molecular Biology 2023Quote: ... five conjugated antibodies were purchased from Akoya Biosciences ...
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bioRxiv - Systems Biology 2023Quote: ... Oligo-conjugated primary antibodies were prepared 50µg at a time using the Akoya Antibody Conjugation Kit (Akoya Biosciences, Delaware, USA) according to the manufacturer’s recommended protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... 30 minute incubation with the secondary antibody (Akoya), 10 minute incubation with the respective OPAL (Akoya ...
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bioRxiv - Cancer Biology 2022Quote: ... 30 minute incubation with the secondary antibody (Akoya), 10 minute incubation with the respective OPAL (Akoya ...
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bioRxiv - Cancer Biology 2023Quote: ... The slides were washed and anti-mouse/anti-rabbit HRP polymer (Akoya Biosciences) was added for 10 min at room temperature ...
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bioRxiv - Genomics 2021Quote: ... Slides were incubated in primary antibody overnight and secondary antibody conjugated to peroxidase and then developed using Tyramide Signal Amplification (TSA, Akoya Biosciences). Slides were counter stained with DAPI ...
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bioRxiv - Cancer Biology 2022Quote: ... Endogenous peroxidase activity was blocked with 3% hydrogen peroxide prior to the addition of any primary antibodies which were prepared in the Opal Blocking/Antibody Diluent (Akoya biosciences). A pan-immune antibody panel and a macrophage subset antibody panel were used to investigate immune cell localization in brain metastasis samples ...
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bioRxiv - Developmental Biology 2024Quote: ... Slides were incubated in primary antibody overnight and secondary antibody conjugated to peroxidase and then developed using Tyramide Signal Amplification (TSA, Akoya Biosciences). Samples were stained with either hematoxylin and eosin or the following antibodies ...
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bioRxiv - Immunology 2022Quote: Commercially available oligonucleotide conjugated antibodies were purchased from Akoya Biosciences and used at a standard dilution of 1/200 (Supplemental Table 1) ...
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bioRxiv - Cancer Biology 2023Quote: Commercially available oligo-conjugated antibodies were obtained from Akoya Biosciences (Marlborough ...
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bioRxiv - Developmental Biology 2024Quote: ... 100 μl of antibody storage buffer (Akoya Biosciences, 7000009) was added on top of antibodies and centrifuged (3000 x g ...
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bioRxiv - Cancer Biology 2021Quote: Tissue sections were baked for 3 hours at 62°C in vertical slide orientation with subsequent deparaffinization performed on the Leica Bond RX followed by 30 minutes of antigen retrieval with Leica Bond ER2 followed by 6 sequential cycles of staining with each round including a 30-minute combined block and primary antibody incubation (Akoya antibody diluent/block ARD1001).
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bioRxiv - Cancer Biology 2023Quote: 4 µm FFPE tissue sections were baked for 2.5 hours at 63°C in vertical slide orientation with subsequent deparaffinization performed on the Leica Bond RX followed by 30 min of antigen retrieval with Leica Bond ER2 followed by 6 sequential cycles of staining with each round including a 30-minute combined block and primary antibody incubation (Akoya antibody diluent/block ARD1001EA) except HER2 ...
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bioRxiv - Immunology 2024Quote: ... Biotinylated antibodies were visualized with Streptavadin-AF594 (Cat. No.: S32356) and the CD4 antibody was visualized with OPAL 520 (Akoya Biosciences, Cat. No.: FP1487001KT). The ROI’s selected to undergo GeoMx® DSP spatial transcriptomics were chosen based on Lyve-1 staining ...
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bioRxiv - Physiology 2022Quote: ... antibodies were reduced using a “Reduction Master Mix” (Akoya Biosciences) to which lyophilized barcodes resuspended in molecular biology grade water and “Conjugation Solution” (Akoya Biosciences ...
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bioRxiv - Physiology 2022Quote: ... Antibodies were imaged cyclically using the CODEX system from Akoya Biosciences and a Keyence BZ-X810 slide scanning microscope fitted with a 20x air objective (0.75 NA) ...
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bioRxiv - Immunology 2021Quote: ... protein blocking in Akoya Antibody Diluent/Block (Akoya biosciences, MA) took place for 10 minutes ...
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bioRxiv - Genomics 2023Quote: ... and incubated with the primary antibody before being probed with an appropriate OPAL™ secondary antibody conjugated to a fluorophore (Akoya Biosciences, Supplementary Table 21). This was followed by a further HIER to remove the primary antibody before staining with the next primary/secondary combination ...
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bioRxiv - Cell Biology 2021Quote: ... Mouse (Cat. No. 468281; Advanced Cell Diagnostics) and Opal 690 Dye (Cat. No. FP1497001KT; Akoya BioSciences) were used according to the manufacturer’s instructions for fluorescence in-situ hybridization ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein blocking was performed using antibody diluent/blocking buffer (Akoya, ARD1001EA) for 10 minutes at room temperature ...