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Citations for Akoya Biosciences :
101 - 141 of 141 citations for Mouse Caspase 2 CASP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... incubation times and appropriate antibody concentrations were optimized using the Opal™ 5-color kit (Akoya Biosciences, NEL840001KT). Epitope stability was assessed to determine the order of each antibody in sequence ...
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bioRxiv - Neuroscience 2023Quote: ... Fluorophore reaction was performed using OPAL 570 and OPAL 650 reaction kits for 10 min at RT (Akoya Biosciences ...
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bioRxiv - Cancer Biology 2021Quote: Multispectral analysis of FFPE tissue utilised the MOTiF™ PD-1/PD-L1 kit (Akoya Biosciences, cat# OP-000001). Staining with Leica BOND RX (Leica Biosystems ...
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bioRxiv - Immunology 2022Quote: ... Tissue sections were deparaffined and rehydrated before multiple-color fluorescent staining using Opal 7-Color IHC Kits (Akoya bioscience). Samples were incubated with primary antibody (1:50 ...
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bioRxiv - Immunology 2022Quote: OPAL multiplex immunofluorescence staining was performed using the OPAL Multiplex 7-color IHC kit (Cat. no. NEL811001KT; Akoya Biosciences), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... the embryos were blocked with the blocking buffer that is included with the TSA Signal Amplification kit from Akoya Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... CA) using probes against the leptin receptor mRNA (Cat# 415951) and Opal 650 dye kit (Akoya Biosciences, Marlborough, MA) to label LepR mRNA ...
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bioRxiv - Cancer Biology 2023Quote: Breast tumour sections were stained with antibodies using the Opal 7-7-colour multiplex immunohistochemistry kit (NEL811001KT, Akoya Biosciences). Staining was performed manually per the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... mIF was performed according to the manufacturer’s protocol using the OPAL Multiplex Manual IHC kit and antigen retrieval (AR) buffers AR6 and AR9 (Akoya Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: ... Multiplex immunofluorescence staining was performed with the Akoya Opal™ Polaris 7-color IHC kit fluorophores (Akoya Biosciences [NEL861001KT]). FFPE tissue sections were baked for 30 minutes ...
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bioRxiv - Immunology 2024Quote: ... Immunofluorescence staining of paraffin embedded tissues were processed using the OPAL 4-Color Manual IHC Kit (Akoya Biosciences, NEL810001KT).
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bioRxiv - Neuroscience 2021Quote: ... Multiplexed immunofluorescent staining was carried out on 4-6µm sections using the Opal Polaris 7 color IHC detection kit (Akoya biosciences) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... The procedure for multiplex immunohistochemistry (mIHC) was followed by a manufacturer’s protocol for Opal7-color automation IHC kit (Akoya Bioscience), and the staining was performed with Autostainer DISCOVERY ULTRA (Ventana) ...
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bioRxiv - Immunology 2020Quote: We stained 5-µm-thick formalin-fixed paraffin-embedded sections from human xenograft tumors (UMSCC-1) using Opal multiplex kits (PerkinElmer/Akoya), for a panel of DAPI ...
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bioRxiv - Neuroscience 2022Quote: ... Bound probes were detected using the RNAscope v2 multiplex fluorescent detection kit and Opal 520 and 690 Dyes (Akoya Biosciences).
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bioRxiv - Immunology 2022Quote: Oligonucleotide barcoded antibody staining of tissue sections mounted on cover slips (n=1 section per tissue region per donor) was performed using a commercially available CODEX Staining Kit according to the manufacturer’s instructions for FFPE tissue (Akoya Biosciences) and as recorded for HuBMAP Lymphatic System TMC SOPs (https://www.protocols.io/view/codex-antibody-staining-protocol-for-ffpe-tissues-bbsdina6) ...
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bioRxiv - Cancer Biology 2022Quote: ... All other antibodies were custom conjugated to their respective CODEX barcode (Supplemental Table 4) according to Akoya’s CODEX user manual using the CODEX Conjugation Kit (Akoya, 7000009). Briefly ...
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bioRxiv - Developmental Biology 2023Quote: ... the DIG-labeled probes were amplified with the Cy3 TSA reagent included with the TSA Signal Amplification kit from Akoya Biosciences ...
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bioRxiv - Developmental Biology 2023Quote: ... which was diluted 1:500 in the same blocking buffer that is included with the TSA Signal Amplification kit from Akoya Biosciences ...
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bioRxiv - Developmental Biology 2023Quote: ... the embryos were washed for five minutes in 500 μL of the 1X amplification diluent included in the TSA Signal Amplification kit from Akoya Biosciences ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by streptavidin-conjugated peroxidase incubation and signal amplification using the TSA Plus Cyanine-3 or -5 kits (Akoya Biosciences).
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bioRxiv - Systems Biology 2023Quote: ... Oligo-conjugated primary antibodies were prepared 50µg at a time using the Akoya Antibody Conjugation Kit (Akoya Biosciences, Delaware, USA) according to the manufacturer’s recommended protocol ...
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bioRxiv - Cell Biology 2024Quote: ... All other antibodies were custom conjugated to their respective CODEX barcode (Supplemental Table S5) according to Akoya’s PhenoCycler-Fusion user guide using the antibody conjugation kit (Akoya, 7000009) following manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: Double fluorescent immunostainings for PARP-1/EpCAM and PARP-1/FRα were performed using Opal Multiplex IHC kit (Akoya Biosciences).
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bioRxiv - Pathology 2024Quote: ... we stained antibodies on tonsil tissue and aorta tissue microarray using the sample kit for PhenoCycler-Fusion (Akoya Biosciences, 7000017) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Staining was performed on a Leica BOND RX autostainer according to the modified Opal 7-color (v5.2 plus) preset protocol using the Opal 6-Plex Detection kit for Whole Slide Imaging (Akoya Biosciences). Prior to the first staining round ...
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bioRxiv - Developmental Biology 2021Quote: ... FISH was performed as described (Brend and Holley 2009; Marra et al., 2017) using TSA Plus Fluorescein or Cyanine Kits (Akoya Biosciences). For all gene expression studies ...
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bioRxiv - Immunology 2021Quote: The antibody panel was composed of commercially available AKOYA-conjugated antibodies and self-conjugated custom antibodies (CODEX Conjugation Kit, AKOYA Biosciences). Tissue staining was performed with the CODEX Staining Kit (AKOYA Biosciences) ...
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bioRxiv - Microbiology 2021Quote: Antigen retrieval and blocking were performed on the dewaxed slides using the Antigen retrieval reagent (pH 6.0) and Blocking/antibody diluent provided in the Opal Polaris Multicolor Manual IHC Detection Kit (Akoya Biosciences, USA), following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Fluorescent RNA in situ hybridization was done with DIG-labeled probes for white and yellow with TSA Plus Fluorescence Kits (Akoya Biosciences) and used for in situ hybridization to imaginal discs as described (Tian et al ...
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bioRxiv - Systems Biology 2023Quote: ... RNA in situ detection was performed using the RNAscope Multiplex Fluorescent V2 Assay kit (Advanced Cell Diagnostics) in combination with OPAL dyes 520 and 570 (Akoya Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... The formalin fixed paraffin embedded bullae were sectioned and processed using Opal Polaris™ 7-color Manual IHC Kit (Akoya Biosciences) following the manufacturer’s recommendation ...
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bioRxiv - Developmental Biology 2023Quote: All fixed frozen tissue CODEX antibody stainings were done according to Akoya Biosciences staining protocol associated with the CODEX Staining Kit with some modifications (Akoya; 7000008). After tissue hydration ...
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bioRxiv - Cancer Biology 2024Quote: mIHC staining was performed according to the manufacturer’s instructions (OpalTM 4-Color Anti-Rabbit Automation IHC; OpalTM Polymer Anti-Rabbit HRP Secondary Antibody Kit; Akoya Biosciences®). The following primary rabbit antibodies were used for staining ...
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bioRxiv - Immunology 2022Quote: ... secondary HRP-labeled antibody and detection with optimized fluorescent Opal tyramide signal amplification (TSA) dye (Opal 7-color Automation IHC kit, from Akoya, Ref. NEL821001KT) and repeated antibody denaturation cycles ...
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bioRxiv - Neuroscience 2021Quote: ... the RNAscope probe for TNFR1 (product #408111) and the TSA Cyanine 3 Plus Evaluation kit (product #NEL744001KT, Akoya Biosciences, Boston, MA, USA). After completing the FISH protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were then washed for 3 x 5 minutes in NTT and developed with Cy5-Tyramide signal amplification reagent (TSA Plus Kit, Akoya Biosciences #NEL745001KT) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: Immunohistochemical staining for insulin and glucagon was conducted using a Tyramide Signal Amplification (TSA) Plus Biotin kit according to the manufacturer’s protocol (NEL700A001KT, Akoya Biosciences, Marlborough, MA, USA), as previously described [15] ...
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bioRxiv - Developmental Biology 2022Quote: ... Signals were amplified using biotin/streptavidin HRP-conjugated secondary Ab (Jackson Immuno, 111-065-003 and 016-030-084) and detected using Tyramide Signal Amplification Plus kit (Akoya Biosciences, NEL744001KT, 1:100). Images were taken using a Leica SP5X laser scanning confocal microscope and further processed using ImageJ Fiji software (Schindelin et al. ...
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bioRxiv - Developmental Biology 2022Quote: ... the samples were incubated in a 1:100 dilution of Cy3-Tyramide Signal Amplification Solution (TSA plus Cyanine 3 Kit, Akoya Biosciences, Marlborough, MA, USA) for 5 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: All antibodies used for multiplexed immunofluorescence were validated individually first by chromogenic singleplex immunohistochemistry (IHC) and then by singleplex immunofluorescence using the Opal 7 kit reagents (NEL871001KT, Akoya Biosciences, Waltham, MA, USA). Detailed methods and antibody information are in supplementary data ...