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Citations for Akoya Biosciences :
51 - 100 of 119 citations for Mouse Anti Hepatitis C Virus NS4b Antibody 1867 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: Tissue sections were baked for 3 hours at 62°C in vertical slide orientation with subsequent deparaffinization performed on the Leica Bond RX followed by 30 minutes of antigen retrieval with Leica Bond ER2 followed by 6 sequential cycles of staining with each round including a 30-minute combined block and primary antibody incubation (Akoya antibody diluent/block ARD1001).
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bioRxiv - Cancer Biology 2023Quote: 4 µm FFPE tissue sections were baked for 2.5 hours at 63°C in vertical slide orientation with subsequent deparaffinization performed on the Leica Bond RX followed by 30 min of antigen retrieval with Leica Bond ER2 followed by 6 sequential cycles of staining with each round including a 30-minute combined block and primary antibody incubation (Akoya antibody diluent/block ARD1001EA) except HER2 ...
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bioRxiv - Immunology 2024Quote: ... Biotinylated antibodies were visualized with Streptavadin-AF594 (Cat. No.: S32356) and the CD4 antibody was visualized with OPAL 520 (Akoya Biosciences, Cat. No.: FP1487001KT). The ROI’s selected to undergo GeoMx® DSP spatial transcriptomics were chosen based on Lyve-1 staining ...
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bioRxiv - Physiology 2022Quote: ... antibodies were reduced using a “Reduction Master Mix” (Akoya Biosciences) to which lyophilized barcodes resuspended in molecular biology grade water and “Conjugation Solution” (Akoya Biosciences ...
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bioRxiv - Physiology 2022Quote: ... Antibodies were imaged cyclically using the CODEX system from Akoya Biosciences and a Keyence BZ-X810 slide scanning microscope fitted with a 20x air objective (0.75 NA) ...
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bioRxiv - Immunology 2021Quote: ... protein blocking in Akoya Antibody Diluent/Block (Akoya biosciences, MA) took place for 10 minutes ...
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bioRxiv - Immunology 2022Quote: ... Antigen retrieval was performed by boiling samples to 100°C for 15 minutes in pH 9 Antigen retrieval buffer (Akoya). Tissues were incubated for 45 minutes at room temperature with primary antibodies specific for CD20 (L26 ...
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bioRxiv - Genomics 2023Quote: ... and incubated with the primary antibody before being probed with an appropriate OPAL™ secondary antibody conjugated to a fluorophore (Akoya Biosciences, Supplementary Table 21). This was followed by a further HIER to remove the primary antibody before staining with the next primary/secondary combination ...
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bioRxiv - Cell Biology 2021Quote: ... Mouse (Cat. No. 468281; Advanced Cell Diagnostics) and Opal 690 Dye (Cat. No. FP1497001KT; Akoya BioSciences) were used according to the manufacturer’s instructions for fluorescence in-situ hybridization ...
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bioRxiv - Molecular Biology 2022Quote: ... The hybridization detection was carried out using Opal Fluorophore Reagent Packs Opal-520 (channel (C) 1)/570 (C2)/690 (C3) (1:1500) (Akoya Biosciences). Probes (Advanced Cell Diagnostics ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were washed for 2 days at 4◦C and then incubated for 5 minutes with TSA Cyanine 5 at room temperature (NEL705A001, Akoya, biosciences). All embryos were nuclei stained with DAPI 1:1000 diluted in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein blocking was performed using antibody diluent/blocking buffer (Akoya, ARD1001EA) for 10 minutes at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... tissue sections were incubated with 1X Antibody Diluent/Block (Akoya Biosciences) for 5 minutes ...
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bioRxiv - Immunology 2023Quote: ... HRP-C1 was applied for 15 min at 40°C after which the slides were washed in wash buffer and incubated with Opal dye 570 (Akoya, OP-001003) diluted 1:1500 in TSA buffer (ACD ...
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bioRxiv - Cell Biology 2024Quote: ... 15 minutes of Protease III (ACD) at 40°C and 1:750 dilution of OPAL570 or OPAL690 reagents (Akoya Biosciences, Marlborough, MA). Immunohistochemistry for claudin-5 co-localization was performed after RNAScope staining ...
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bioRxiv - Immunology 2022Quote: ... Primary antibodies were detected with OPAL Polymer HRP Ms + Rb (Akoya Biosciences) for 15 minutes and visualized following a 10-minute incubation with Tyramide signal amplification and Opal Fluorophore (Akoya ...
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bioRxiv - Immunology 2023Quote: ... Primary antibodies were diluted in 1X Plus Automation Amplification Diluent (Akoya Biosciences). Primaries were detected using according anti-species secondary antibodies conjugated to HRP (OmniMap Ventana ...
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bioRxiv - Cancer Biology 2024Quote: ... Scanning of the full antibody panel was performed on PhenoImager (Akoya Biosciences). A spectral fluorophore for each primary antibody and autofluorescence library was made to enable optimal multispectral unmixing ...
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bioRxiv - Neuroscience 2024Quote: ... after which the hybridized probes were detected using peroxidase conjugated anti-FLU or anti-DIG Fab(2) fragments and the Tyramide signal amplification (TSA) system from Akoya Biosciences ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Secondary antibodies were obtained from Akoya (OPAL 690, OPAL 520, and OPAL 570) and diluted to a concentration of 1:750 ...
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bioRxiv - Cancer Biology 2023Quote: ... panCK staining was performed by using an OPAL TSA-DIG antibody (Akoya Biosciences) in combination with an OPAL 780 fluorophore conjugated anti-DIG antibody ...
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bioRxiv - Cancer Biology 2020Quote: ... CODEX staining and imaging was performed on pancreatic mouse tumors utilizing this panel following the manufacturer’s protocol (Akoya Bioscience). Raw fluorescent TIFF image files were processed ...
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bioRxiv - Immunology 2023Quote: ... Mixed RNAscope (Advanced Cell Diagnostics)/antibody antigen retrieval and staining with Opal (Akoya Biosciences) fluorophores was performed manually following the RNAscope Multiplex Fluorescent v2 Assay combined with the immunofluorescence protocol (322818-TN ...
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bioRxiv - Pathology 2024Quote: ... To validate the efficacy of commercial DNA-barcode-conjugated antibodies for PhenoCycler by Akoya Biosciences ...
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bioRxiv - Neuroscience 2022Quote: ... Each probe was then developed by sequential application of the appropriate HRP reagent (HRP-C1, HRP-C2) for 15 minutes each at 40°C and fluorophore (Akoya Biosciences Opal 690 and Opal 520) for 30 minutes each at 40°C ...
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bioRxiv - Immunology 2023Quote: ... The tissue section was stained with a cocktail of DNA-barcoded antibodies purchased from Akoya company (CD4/EPR6855 ...
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bioRxiv - Bioengineering 2023Quote: The antibody conjugation and the staining were done according to the manufacturer’s protocol (Akoya Biosciences). Details regarding primary antibody ...
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bioRxiv - Cancer Biology 2024Quote: ... Signal for antibody complexes was labeled and visualized by their corresponding Opal Fluorophore Reagents (Akoya) by incubating the slides for 10 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-Cytokeratin 5 (1:300 dilution) with Opal 690 (Akoya Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... Opal Anti-Ms + Rb HRP (Akoya Biosciences, Marlborough, MA ARH1001EA), before conjugating with Opal fluorophores Following incubation ...
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bioRxiv - Immunology 2021Quote: ... antibodies was performed using the OPAL Automation Multiplex IHC Detection Kit (Akoya Biosciences, Catalog 160 #NEL830001KT) implemented onto a BOND Research Detection System (DS9455) ...
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bioRxiv - Neuroscience 2023Quote: ... followed by a 10-minute incubation with Opal Antibody Dilutent/Block solution (Akoya Bioscience, Marlborough, USA). The first primary antibody was then applied overnight at 4°C ...
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bioRxiv - Physiology 2022Quote: 14 of the 23 antibodies used here were conjugated in-house using the protocol outlined by Akoya Biosciences (Table 3)19,20 ...
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bioRxiv - Molecular Biology 2021Quote: ... The binding sites of HRP-conjugated secondary antibodies were detected with Red Opal (1:100, AKOYA Biosciences) and a DAPI counterstain was applied (1:1000) ...
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bioRxiv - Immunology 2021Quote: ... RNA probes and panCK antibody were detected with the following Opal Fluorophores pairings: CXCL13 Opal 480 (Akoya FP1500001KT), CXCL10/11 Opal 520 (Akoya FP1487001KT) ...
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bioRxiv - Immunology 2022Quote: ... Each target was linked using the OPAL Polymer HRP conjugated secondary antibodies (ready-to-use, ARH1001EA, AKOYA Biosciences). Visualization of the different targets was established using the OPAL Polaris 570 (FP1488001KT ...
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bioRxiv - Cancer Biology 2023Quote: ... incubation times and appropriate antibody concentrations were optimized using the Opal™ 5-color kit (Akoya Biosciences, NEL840001KT). Epitope stability was assessed to determine the order of each antibody in sequence ...
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bioRxiv - Cancer Biology 2022Quote: ... Slides were washed in TBS + 0.1% tween (TBS-T) and blocked in Antibody Diluent/Block (Akoya Biosciences, ARD1001EA) for 30 minutes at RT ...
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bioRxiv - Cell Biology 2022Quote: ... The binding of antibodies were detected with a 1:150 dilution of OPAL 520 Reagent (AKOYA, SKU FP1487001KT). Slides were digitally imaged using an Aperio ScanScope FL Scanner (Leica Biosystems).
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bioRxiv - Immunology 2022Quote: ... with OmniMap anti-rabbit-HRP and Opal 690 fluorophore (Akoya Biosciences, 1:50). Finally ...
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bioRxiv - Immunology 2022Quote: ... with OmniMap anti-rabbit-HRP and Opal 570 fluorophore (Akoya Biosciences, 1:1000), and lastly CD3 (abcam ...
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bioRxiv - Cancer Biology 2021Quote: ... staining was performed at 1:15,000 dilution followed by secondary antibody 570 opal reagent (1:100 dilution) (Akoya Biosciences) and peak wavelength at 555/570 ...
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bioRxiv - Neuroscience 2023Quote: ... Detection of probe and antibody were performed using Tyramide Signal Amplification system with Opal 570 or 520 reagents (Akoya Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: Breast tumour sections were stained with antibodies using the Opal 7-7-colour multiplex immunohistochemistry kit (NEL811001KT, Akoya Biosciences). Staining was performed manually per the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Then a staining dye was added after secondary antibody washes: α-SMA – Opal-dye 520 (Akoya Biosciences OP-001001) at 1:100 ...
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bioRxiv - Cancer Biology 2021Quote: ... staining was performed at 1:100 dilution followed by secondary antibody 520 reagent (1:100) (Akoya Biosciences; Menlo Park, California) and peak wavelength at 494/525 ...
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bioRxiv - Genomics 2022Quote: A total of 32 antibodies were used for CODEX and 19 of them were conjugated in-house using a protocol outlined by Akoya Biosciences (Akoya Biosciences ...
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bioRxiv - Neuroscience 2022Quote: FFPE tissue sections containing OB and OT were stained consecutively with specific primary antibodies according to standard protocols provided by Akoya and performed routinely by the HIMSR ...
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bioRxiv - Pathology 2023Quote: ... secondary antibodies labeled with horseradish peroxidase (all with reduced cross-reactivity from Jackson-Immunoresearch) in combination with 7plex Opal system (Akoya) were used according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Additional antibodies were obtained from commercial vendors as indicated and conjugated to custom oligo barcodes according to instructions in Akoya’s antibody conjugation kit (Conjugation kit, Akoya, #7000009). Briefly ...