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Citations for Akoya Biosciences :
1 - 50 of 122 citations for Mouse Anti Canine Parvovirus 2 Antibody 3H6 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... A mixture of anti-Mouse + anti-Rabbit HRP-conjugated secondary antibodies (Akoya Biosciences) was added for 10 minutes ...
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bioRxiv - Immunology 2023Quote: ... Antibody binding was visualized using secondary anti-mouse/anti-rabbit polymer HRP followed with TSA-Opal reagents (Akoya NEL811001KT). Tissue slides were incubated with DAPI as a counterstain and coverslipped with VectaShield mounting media (Vector Labs) ...
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bioRxiv - Neuroscience 2021Quote: ... HRP-conjugated anti-flu antibody (AKOYA Bioscience NEF710001EA ...
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bioRxiv - Cancer Biology 2023Quote: ... The slides were washed and anti-mouse/anti-rabbit HRP polymer (Akoya Biosciences) was added for 10 min at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... Anti-mouse and anti-rabbit Opal Polymer Horseradish Peroxidase (Opal Polymer HRP Ms + Rb, Akoya Biosciences, Cat. ARH1001EA) were then applied as a secondary label for 10 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... after which the hybridized probes were detected using peroxidase conjugated anti-FLU or anti-DIG Fab(2) fragments and the Tyramide signal amplification (TSA) system from Akoya Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... slides were incubated with anti-rabbit HRP-conjugated secondary antibody (Akoya Biosciences, NEL840001KT). Sections underwent rounds of antigen stripping via incubation at 100 °C in AR9 buffer (Akoya Biosciences ...
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bioRxiv - Neuroscience 2024Quote: ... HRP-conjugated anti-flu antibody (at a dilution of 1:250; #NEF710001EA, AKOYA Bioscience) was used to detect Flu-labeled RNA probes by a 1:70 TSA-plus biotin (#NEL749A001KT ...
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bioRxiv - Cancer Biology 2024Quote: mIHC staining was performed according to the manufacturer’s instructions (OpalTM 4-Color Anti-Rabbit Automation IHC; OpalTM Polymer Anti-Rabbit HRP Secondary Antibody Kit; Akoya Biosciences®). The following primary rabbit antibodies were used for staining ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were then blocked with 10% BSA + 0.05% Tween20 and the antibodies were diluted in 2% BSA in 0.05% PBST and used in a multiplexed manner with OPAL reagents (Akoya Biosciences). All primary antibodies (described above for western blotting ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were then blocked with 10% BSA + 0.05% Tween20 and the antibodies were diluted in 2% BSA in 0.05% PBST and used in a multiplexed manner with OPAL reagents (Akoya Biosciences). All primary antibodies were incubated overnight at 4 °C ...
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bioRxiv - Bioengineering 2022Quote: ... All sections were then incubated with an Opal 520 polymer anti-rabbit HRP antibody (Akoya Biosciences) and counterstained with DAPI ...
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bioRxiv - Microbiology 2023Quote: ... Each antibody was optimized using Opal 3-Plex Anti-Rb Detection Kit (Akoya Biosciences Inc. cat# NEL830001KT) and stained with automated LabSatTM Research (Lunaphore Technologies SA ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were then incubated with HRP conjugated goat anti rabbit (1:100, Table S1) diluted 2% NHS for 1.5hr followed by OPAL 570 (1:500, Akoya FP1488001KT) reagent incubation for 15 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were then incubated with HRP conjugated goat anti rabbit (1:100, Table S1) diluted 2% NHS for 1.5hr followed by OPAL 650 (1:500, Akoya FP1496001KT) reagent incubation for 15 min ...
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bioRxiv - Cancer Biology 2023Quote: ... All antibodies (including pre-conjugated antibodies from Akoya) were additionally validated on the respective GBM control samples using multiple CODEX runs to adjust antibody concentration and exposure times to optimize signal to noise ratio ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated with HRP-conjugated anti-DIG (1:500) antibody overnight and treated with TSA-plus biotin (NEL749A001KT; Akoya Biosciences), followed by streptavidin-Alexa Fluor 488 (S32354 ...
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bioRxiv - Developmental Biology 2023Quote: ... The samples were then washed with Tris-NaCl-Tween Buffer after incubation with anti-DIG-POD antibodies and incubated with Cy3-tyramide solution (TSA Plus Cyanine 3; AKOYA Biosciences, NEL744001KT) for 10 min ...
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bioRxiv - Cancer Biology 2023Quote: ... antibodies were conjugated using Akoya Antibody Conjugation Kit (Akoya Biosciences, SKU 7000009) with a barcode (Akoya Biosciences ...
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bioRxiv - Immunology 2022Quote: ... the HRP-coupled secondary anti-rat OmniMap antibody (Ventana Medical Systems) for 12 min and finally Opal 520 fluorophore (Akoya Biosciences, 1:100) at RT for 8 min ...
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bioRxiv - Immunology 2021Quote: The antibody panel was composed of commercially available AKOYA-conjugated antibodies and self-conjugated custom antibodies (CODEX Conjugation Kit, AKOYA Biosciences). Tissue staining was performed with the CODEX Staining Kit (AKOYA Biosciences) ...
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bioRxiv - Developmental Biology 2023Quote: ... The probes were for Mus musculus Fgf10 (channel 2, 446371) and Wnt5a (channel 2, 316791) and the fluorophores were Opal Polaris 520 and Opal 620 (Akoya Biosciences FP1487001KT and FP1495001KT) ...
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bioRxiv - Cancer Biology 2022Quote: ... 6-diamidino-2-phenylindole (Spectral DAPI, Akoya Biosciences) was applied per provided protocols to label nuclei ...
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bioRxiv - Systems Biology 2023Quote: ... 6-diamidino-2-phenylindole (Spectral DAPI, Akoya Biosciences) was applied per provided protocols to label nuclei ...
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bioRxiv - Cancer Biology 2023Quote: ... NRG1 and TGFa primary antibodies were detected with HRP-conjugated antibody (ARR1001KT, Akoya Biosciences) and TSA-based amplification with Opal dye 690 (FP1497001KT ...
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bioRxiv - Immunology 2022Quote: ... conjugated primary antibodies cocktail (Akoya CD3e 1:100 and Akoya CD20 1:200 in Abcam Antibody Diluent ...
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bioRxiv - Molecular Biology 2023Quote: For antibodies purchased from Akoya Biosciences ...
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bioRxiv - Cell Biology 2021Quote: Antibodies were purchased preconjugated from Akoya Biosciences or sourced from other vendors and conjugated in-house using the CODEX Conjugation Kit (Akoya Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: Antibody conjugation requires reagents from Akoya Biosciences ...
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bioRxiv - Immunology 2022Quote: ... conjugated primary antibodies cocktail (Akoya CD3e 1:100 and Akoya CD20 1:200 in Abcam Antibody Diluent) 2x for 90 minutes each ...
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bioRxiv - Developmental Biology 2024Quote: The high-parametric antibody panel consisted of both a selection of pre-conjugated antibodies (n=23, Akoya Biosciences) and antibodies available through other commercial sources that were conjugated in house (n=49 ...
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bioRxiv - Cell Biology 2022Quote: ... a secondary HRP antibody (ARH1001EA, AKOYA Biosciences) followed by Opal 520 fluorophore (FP1487001KT ...
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bioRxiv - Neuroscience 2022Quote: ... diluted in Antibody Diluent/Block (Akoya, ARD1001EA) for 30min at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... Opal Polymer HRP secondary antibody (AKOYA Biosciences), Opal Fluorophore (AKOYA Biosciences) ...
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bioRxiv - Cancer Biology 2023Quote: ... All antibodies not commercially available from Akoya and hence requiring custom-conjugation with DNA-barcodes were first validated with conventional immunofluorescence (Supplementary Note 2) ...
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bioRxiv - Molecular Biology 2023Quote: ... five conjugated antibodies were purchased from Akoya Biosciences ...
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bioRxiv - Systems Biology 2023Quote: ... Oligo-conjugated primary antibodies were prepared 50µg at a time using the Akoya Antibody Conjugation Kit (Akoya Biosciences, Delaware, USA) according to the manufacturer’s recommended protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... 30 minute incubation with the secondary antibody (Akoya), 10 minute incubation with the respective OPAL (Akoya ...
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bioRxiv - Cancer Biology 2022Quote: ... 30 minute incubation with the secondary antibody (Akoya), 10 minute incubation with the respective OPAL (Akoya ...
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bioRxiv - Cancer Biology 2022Quote: Stained coverslips were mounted onto the CODEX stage plate version 2 (Akoya) and secured onto the stage of a BZ-X810 inverted fluorescence microscope (Keyence) ...
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bioRxiv - Genomics 2021Quote: ... Slides were incubated in primary antibody overnight and secondary antibody conjugated to peroxidase and then developed using Tyramide Signal Amplification (TSA, Akoya Biosciences). Slides were counter stained with DAPI ...
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bioRxiv - Cancer Biology 2022Quote: ... Endogenous peroxidase activity was blocked with 3% hydrogen peroxide prior to the addition of any primary antibodies which were prepared in the Opal Blocking/Antibody Diluent (Akoya biosciences). A pan-immune antibody panel and a macrophage subset antibody panel were used to investigate immune cell localization in brain metastasis samples ...
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bioRxiv - Developmental Biology 2024Quote: ... Slides were incubated in primary antibody overnight and secondary antibody conjugated to peroxidase and then developed using Tyramide Signal Amplification (TSA, Akoya Biosciences). Samples were stained with either hematoxylin and eosin or the following antibodies ...
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bioRxiv - Genomics 2024Quote: ... Slides were incubated for 1 hour at room temperature with primary antibodies diluted in 1x Opal blocking/antibody diluent (Akoya biosciences). Slides were subsequently incubated with the Opal Polymer HRP Ms + Rb secondary polymer for 30 minutes prior to incubation with Opal fluorophores (Opal 520 ...
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bioRxiv - Immunology 2021Quote: ... followed by incubation with the first primary antibody for 1 hour, subsequently with the secondary antibody (Polymer HRP, Ms + Rb (Akoya biosciences, MA)) for 30 minutes and finally with an Opal fluorophore ((Akoya biosciences ...
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bioRxiv - Immunology 2022Quote: Commercially available oligonucleotide conjugated antibodies were purchased from Akoya Biosciences and used at a standard dilution of 1/200 (Supplemental Table 1) ...
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bioRxiv - Cancer Biology 2023Quote: Commercially available oligo-conjugated antibodies were obtained from Akoya Biosciences (Marlborough ...
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bioRxiv - Developmental Biology 2024Quote: ... 100 μl of antibody storage buffer (Akoya Biosciences, 7000009) was added on top of antibodies and centrifuged (3000 x g ...
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bioRxiv - Cancer Biology 2021Quote: ... the slides were counterstained with 4’,6-diamidino-2-phenylindole (DAPI, Akoya Biosciences) and coverslipped using ProLong Gold antifade mounting media (Thermo Fisher Scientific) ...
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bioRxiv - Pathology 2021Quote: IHC tissues were imaged with a Mantra 2 Quantitative Pathology Workstation (Akoya Biosciences) with a 20x objective ...