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Citations for Akoya Biosciences :
151 - 200 of 207 citations for Laminin Alpha 1 LAMA1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... slides were incubated with Opal fluorophore-conjugated tyramide signal amplification (TSA; 1:100, Akoya Biosciences). After the application of tyramides ...
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bioRxiv - Neuroscience 2022Quote: ... followed by tyramide-conjugated Cy3 (1:50) diluted in TSA amplification diluents (Akoya Biosciences, NEL741001KT). Sections were mounted ...
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bioRxiv - Developmental Biology 2023Quote: ... Signal amplification was done with 1:50 TSA Plus Cyanine-3 or -5 (Akoya Biosciences) for 1 h at RT ...
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bioRxiv - Neuroscience 2024Quote: ... The signals were amplified by a 1:70 TSA-plus Cyanine 3 (#NEL744001KT; AKOYA Bioscience) for 25 minutes ...
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bioRxiv - Immunology 2021Quote: ... and PanCK was performed using the Motif PD-1/PD-L1 panel (Akoya Biosciences OP-000001) on a Leica Bond Rx instrument (Leica Biosystems ...
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bioRxiv - Neuroscience 2022Quote: ... and 1hr incubation with TSA plus Cyanine-3 (1:300; cat# NEL744001KT; Akoya Biosciences, Marlborough, MA) was performed ...
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bioRxiv - Microbiology 2022Quote: ... The following fluorophores were used during incubations: Opal 520 Reagent Pack (1:1500; Akoya Biosciences, FP1487001KT) for COX I detection ...
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bioRxiv - Neuroscience 2023Quote: ... slices were incubated in tyramide conjugated fluorescein (1:50) in TSA amplification diluent (Akoya Biosciences, NEL741001KT) for 10min at RT ...
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bioRxiv - Genomics 2020Quote: ... Fluorescent labeling was conducted using Opal 480 (GFAP) and Opal 570 (CD44) dyes (1:150; Akoya Biosciences) for 10 minutes at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... Tyramide Signal Amplification (TSA) solutions were prepared as follows: 1/500 for TSA-Fluorescein (Akoya Biosciences, NEL741001KT), 1/2000 for TSA-CY3 (Akoya Biosciences ...
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bioRxiv - Neuroscience 2022Quote: ... All probes were detected using a 1:750 dilution of TSA plus fluorescein 488 (Akoya Biosciences NEL741001KT). Slides were then dried overnight ...
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bioRxiv - Neuroscience 2022Quote: ... washed again (2 x 5 min) and incubated with OPAL 650 Tyramide (Akoya Biosciences, FP1496001KT, 1:500) for 10 minutes ...
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bioRxiv - Immunology 2023Quote: ... Sections were stained with probes against CCL2 (channel 1; ACD 423811) conjugated to Opal 690 (Akoya FP1497001KT), CDKN1A (channel 2 ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-ER (Cell Marque, 249-R-15-ASR, clone: SP1, 1:70 dilution) with Opal 650 (Akoya Biosciences ...
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bioRxiv - Cell Biology 2024Quote: ... The ER was then visualized incubating the cells using fluorescent tyramide (TSA-FITC 1:200, AKOYA Biosciences), 0.003 H202 and 0,05% Tween-20 in 100 mM borate buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... and SARS-CoV-2 spike mRNA with TSA Plus CY5 (1:1500, #NEL745001KT, Akoya Biosciences, MA, USA). Fluorescent images were acquired with NanoString GeoMX DSP at 20×.
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bioRxiv - Neuroscience 2024Quote: ... FITC detection was performed using TSA Plus fluorescein diluted 1:50 in amplification diluent (NEL741001KT, Akoya Biosciences). Samples were washed several times in TNT ...
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bioRxiv - Developmental Biology 2021Quote: ... Slices were covered by 80 μL of Opal 650 at 1/1000 (Opal 650 reagent pack, AKOYA Biosciences) during 30 min at 40°C and washed at RT with RNAscope wash buffer two times ...
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bioRxiv - Neuroscience 2022Quote: ... Hybridized slides were incubated with amplification reagents and Opal 570 and 690 dyes (Akoya Biosciences, 1:1500 dilution) followed by sequential incubation with tyrosine hydroxylase (Aves Labs ...
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bioRxiv - Developmental Biology 2023Quote: ... sections were incubated for 30 minutes with 1:200 Cy3-Tyramide in TSA amplification buffer (Akoya Biosciences®), at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... the FITC-labeled probe was visualized using tyramide-FITC Plus (1/50 dilution, 15-minute incubation, Akoya Biosciences). Following washes in TNT ...
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bioRxiv - Neuroscience 2024Quote: ... was used to detect Flu-labeled RNA probes by a 1:70 TSA-plus biotin (#NEL749A001KT; AKOYA Bioscience), which was then visualized with streptavidin-AF488 (at a dilution of 1:250 ...
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bioRxiv - Immunology 2023Quote: ... Counterstaining was performed with DAPI for 5 min at room temperature (1 drop per 500 µL, Akoya Bioscience). Microscope images were taken using the Zeiss AxioImager M2 and analyzed using ZEN blue software (v2.3).
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bioRxiv - Cancer Biology 2021Quote: Multispectral analysis of FFPE tissue utilised the MOTiF™ PD-1/PD-L1 kit (Akoya Biosciences, cat# OP-000001). Staining with Leica BOND RX (Leica Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: ... 1:200 in TNB blocking buffer) for 45 min followed by DAPI staining for 5 min (Spectral DAPI, Akoya Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... The fluorophores applied to detect the signal from the probes were Opal 690 Reagent (1:750; Akoya Biosciences, #FP1497001KT) for C1 and Opal 570 Reagent (1:750 ...
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bioRxiv - Developmental Biology 2023Quote: ... TSA plus cyanine 3 solution (dilute TSA-Cy3 1:250 in 100mM Boric Acid, pH8.5 containing 0.003% H2O2) (Akoya Biosciences) was added to the tissue sections and incubated for 10 min at RT ...
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bioRxiv - Neuroscience 2021Quote: ... the C4 probe signal was developed as above but with HRP-C4 and Opal 620 dye (Akoya Biosciences; 1:1500). Slides were coverslipped with ProLong Gold Antifade Mountant (Invitrogen™ ...
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bioRxiv - Cell Biology 2021Quote: ... For amplified targets the appropriate HRP-conjugated secondary was then incubated at 1:250 in PBS for 45 minutes-1hr followed by TSA fluorophore treatment (Akoya). For unamplified targets appropriate Cy3 or FITC labeled secondary antibodies were used at 1:100 ...
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bioRxiv - Immunology 2020Quote: We stained 5-µm-thick formalin-fixed paraffin-embedded sections from human xenograft tumors (UMSCC-1) using Opal multiplex kits (PerkinElmer/Akoya), for a panel of DAPI ...
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bioRxiv - Cancer Biology 2023Quote: ... the sections were incubated in Opal 520 diluted in blocking buffer (for CK19 and CD8 primary) (Akoya Biosciences, 1:100) for 15 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... which was diluted 1:500 in the same blocking buffer that is included with the TSA Signal Amplification kit from Akoya Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... ISH was completed by washing slides 3x for 15 minutes in PBS before incubation in Polaris 780 (1:125 in diluent buffer, Akoya) for 30 minutes at 40C ...
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bioRxiv - Neuroscience 2023Quote: ... Final detection was achieved with the Opal-570 and Opal-690 fluorophore reagent packs (Akoya BioSciences, Inc.,diluted 1:1000), and samples were conterstained with DAPI (ACD ...
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bioRxiv - Neuroscience 2024Quote: ... Opal dyes (1:1000) were assigned for each channel: Vgat expression was identified by Opal 520 (Akoya Bioscience, catalog # FP1487001KT), Npy expression was identified by Opal 570 (Akoya Bioscience ...
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Wnt-dependent activation of ERK mediates repression of chondrocyte fate during calvarial developmentbioRxiv - Developmental Biology 2021Quote: ... The slides were then washed in 1X TBS-T and incubated in HRP-conjugated goat-anti-rabbit secondary (1:1500, PerkinElmer NEF812001EA, Akoya Biosciences) for 45 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... a working Opal dye solution was made fresh using a 1:500 ratio of Opal dye (Akoya Biosciences, Marlborough, MA, USA) to TSA buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... then covered by 80 μL of Opal 520 at 1/1500 (Opal 520 reagent pack, FP1487001KT, AKOYA Biosciences, Malborough, United States) during 30 min at 40°C and washed at RT with RNAscope wash buffer two times ...
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bioRxiv - Developmental Biology 2022Quote: ... the sections were further incubated with 1:200 TSA Plus FITC or Cy3 reagents for 3-5 minutes of signal development (NEL774001KT or NEL771B001KT, Akoya Bioscience). Sections were then counterstained with 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Cancer Biology 2022Quote: ... Immunofluorescence was visualized using Opal fluorophores (Opal 520, 540, 570, 620, 650 and 690) diluted at 1:150 in a Plus Automation Amplification Diluent (Akoya biosciences). Serial multiplexing was performed by repeating the sequence of antigen retrieval ...
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bioRxiv - Cancer Biology 2023Quote: ... 690 fluorophores according to the protocol using 1:750 dilution of each fluorophore (Cat #FP1488001, #FP1497001, #FP1501001, Akoya Biosciences, Marlborough, MA). Slides were then mounted using Prolong Gold anti-Fade mounting media with DAPI and imaged at 600X magnification using the Nikon Ti2-E inverted microscope system (Nikon ...
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bioRxiv - Neuroscience 2023Quote: ... After the described washes and blocking steps the DIG-labeled probes were visualized using tyramide-Cy3 Plus (1/75 dilution, 15-minute incubation, Akoya Biosciences). After washes in TNT ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue was washed several more times in TNT and DIG detection was performed using TSA Plus Cyanine diluted 1:50 in amplification diluent (NEL744001KT, Akoya Biosciences). Fluorescently labeled samples were imaged using confocal microscopy.
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bioRxiv - Genomics 2024Quote: ... Slides were subsequently incubated with the Opal Polymer HRP Ms + Rb secondary polymer for 30 minutes prior to incubation with Opal fluorophores (Opal 520, 540, 570, 620, 650 and 690) diluted at 1:150 in 1x Plus Automation Amplification Diluent (Akoya biosciences) for 10 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... Probe remaining on the tissue sections was amplified and labeled with a single Opal 520 fluorophore at a dilution of 1:750 (OP-001001, Akoya Biosciences, Marlborough). After RNA labeling ...
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bioRxiv - Neuroscience 2023Quote: ... sections underwent signal amplification steps and fluorophore (1:750 dilutions of Opal™ Dyes: 520 cat# FP1487001KT, 570 cat# 1488001KT, 690 cat# 1497001KT; Akoya Biosciences) treatments according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... probes were amplified and fluorescently labeled with Opal dyes as follows: PROX1 was labeled with 1:500 Opal 520 (catalog no. FP1487001KT, Akoya Biosciences [AB]), LAMP5 was labeled with 1:500 Opal 690 (catalog no ...
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bioRxiv - Cell Biology 2024Quote: ... 15 minutes of Protease III (ACD) at 40°C and 1:750 dilution of OPAL570 or OPAL690 reagents (Akoya Biosciences, Marlborough, MA). Immunohistochemistry for claudin-5 co-localization was performed after RNAScope staining ...
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bioRxiv - Neuroscience 2021Quote: ... The embryos are incubated with the 1:1,000 anti-digoxigenin-POD antibody overnight at 4°C and amplified with Tyramide Amplification working solution and exposed with fluorescein (1:150 dilution of TSA stock with 1x Plus Amplification Diluent-fluorescein) (Akoya Biosciences, Marlborough, MA). All FISH embryos were mounted using DAPI in MOPs buffer and NucBlue (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... for 1 hour at room temperature and amplified with Tyramide Amplification working solution (1:150 dilution of TSA stock with 1x Plus Amplification Diluent-fluorescence) (Akoya Biosciences, Marlborough, MA). Cells were mounted in VectaShield Anti-Fade mounting media with DAPI (Vector Laboratories ...