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Citations for Akoya Biosciences :
151 - 200 of 242 citations for Human Lymphoid Enhancer Binding Factor 1 LEF1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: Oligonucleotide barcoded antibody staining of tissue sections mounted on cover slips (n=1 section per tissue region per donor) was performed using a commercially available CODEX Staining Kit according to the manufacturer’s instructions for FFPE tissue (Akoya Biosciences) and as recorded for HuBMAP Lymphatic System TMC SOPs (https://www.protocols.io/view/codex-antibody-staining-protocol-for-ffpe-tissues-bbsdina6) ...
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bioRxiv - Cancer Biology 2022Quote: ... All other antibodies were custom conjugated to their respective CODEX barcode (Supplemental Table 4) according to Akoya’s CODEX user manual using the CODEX Conjugation Kit (Akoya, 7000009). Briefly ...
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bioRxiv - Developmental Biology 2023Quote: ... the DIG-labeled probes were amplified with the Cy3 TSA reagent included with the TSA Signal Amplification kit from Akoya Biosciences ...
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bioRxiv - Developmental Biology 2023Quote: ... the embryos were washed for five minutes in 500 μL of the 1X amplification diluent included in the TSA Signal Amplification kit from Akoya Biosciences ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by streptavidin-conjugated peroxidase incubation and signal amplification using the TSA Plus Cyanine-3 or -5 kits (Akoya Biosciences).
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bioRxiv - Systems Biology 2023Quote: ... Oligo-conjugated primary antibodies were prepared 50µg at a time using the Akoya Antibody Conjugation Kit (Akoya Biosciences, Delaware, USA) according to the manufacturer’s recommended protocol ...
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bioRxiv - Pathology 2024Quote: ... we stained antibodies on tonsil tissue and aorta tissue microarray using the sample kit for PhenoCycler-Fusion (Akoya Biosciences, 7000017) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... All other antibodies were custom conjugated to their respective CODEX barcode (Supplemental Table S5) according to Akoya’s PhenoCycler-Fusion user guide using the antibody conjugation kit (Akoya, 7000009) following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Staining was performed on a Leica BOND RX autostainer according to the modified Opal 7-color (v5.2 plus) preset protocol using the Opal 6-Plex Detection kit for Whole Slide Imaging (Akoya Biosciences). Prior to the first staining round ...
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bioRxiv - Neuroscience 2020Quote: ... The C4 probe complexes were first incubated with TSA–Biotin (Akoya NEL700A001KT, 1:250) for 30 min at RT ...
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bioRxiv - Developmental Biology 2022Quote: ... The C4 probe complexes were first incubated with TSA–Biotin (Akoya NEL700A001KT, 1:250) for 30 min at RT ...
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bioRxiv - Cancer Biology 2021Quote: ... for 12 minutes and with Opal 780 reactive fluorophore for 1 hour (Akoya Biosciences). Before each round of staining ...
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bioRxiv - Neuroscience 2021Quote: ... fos mRNA was then tagged to the fluorophore fluorescein (1:1500; NEL741E001KT, Akoya Biosciences), and v1aR-mRNA was tagged to the fluorophore Cy5 (1:1500 ...
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bioRxiv - Neuroscience 2021Quote: ... and v1aR-mRNA was tagged to the fluorophore Cy5 (1:1500; NEL745E001KT, Akoya Biosciences). Slides were then rinsed in TBS and stained with a fluorescent Nissl (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were treated with TSA-plus Cyanine 3 (NEL744001KT; Akoya Biosciences; 1:1500). Fluoromount (K024 ...
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bioRxiv - Neuroscience 2023Quote: ... Cy3 or fluorescein at 1:1000 in TSA buffer (TSATM Plus System, Akoya Bioosciences). Following that slides were incubated in HRP blocker for 15 min and then rinsed 3 times with wash buffer reagent ...
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bioRxiv - Cancer Biology 2023Quote: ... the slides were developed with Opal fluorescent dye at 1:50 dilution (Akoya Biosciences) in different channels ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by incubation in Opal 690 reagent for 15 minutes (Akoya Biosciences, 1:100) in blocking buffer ...
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bioRxiv - Neuroscience 2023Quote: ... HRP-C2 and HRP-C3 were labelled with Opal 520 (1:750, Akoya FP1487001KT) and Opal 570 (1:1500 ...
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bioRxiv - Neuroscience 2024Quote: ... HRP-conjugated anti-flu antibody (at a dilution of 1:250; #NEF710001EA, AKOYA Bioscience) was used to detect Flu-labeled RNA probes by a 1:70 TSA-plus biotin (#NEL749A001KT ...
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bioRxiv - Developmental Biology 2021Quote: ... FISH was performed as described (Brend and Holley 2009; Marra et al., 2017) using TSA Plus Fluorescein or Cyanine Kits (Akoya Biosciences). For all gene expression studies ...
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bioRxiv - Immunology 2021Quote: The antibody panel was composed of commercially available AKOYA-conjugated antibodies and self-conjugated custom antibodies (CODEX Conjugation Kit, AKOYA Biosciences). Tissue staining was performed with the CODEX Staining Kit (AKOYA Biosciences) ...
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bioRxiv - Microbiology 2021Quote: Antigen retrieval and blocking were performed on the dewaxed slides using the Antigen retrieval reagent (pH 6.0) and Blocking/antibody diluent provided in the Opal Polaris Multicolor Manual IHC Detection Kit (Akoya Biosciences, USA), following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Fluorescent RNA in situ hybridization was done with DIG-labeled probes for white and yellow with TSA Plus Fluorescence Kits (Akoya Biosciences) and used for in situ hybridization to imaginal discs as described (Tian et al ...
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bioRxiv - Systems Biology 2023Quote: ... RNA in situ detection was performed using the RNAscope Multiplex Fluorescent V2 Assay kit (Advanced Cell Diagnostics) in combination with OPAL dyes 520 and 570 (Akoya Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: All fixed frozen tissue CODEX antibody stainings were done according to Akoya Biosciences staining protocol associated with the CODEX Staining Kit with some modifications (Akoya; 7000008). After tissue hydration ...
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bioRxiv - Cancer Biology 2024Quote: ... The formalin fixed paraffin embedded bullae were sectioned and processed using Opal Polaris™ 7-color Manual IHC Kit (Akoya Biosciences) following the manufacturer’s recommendation ...
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bioRxiv - Cancer Biology 2024Quote: mIHC staining was performed according to the manufacturer’s instructions (OpalTM 4-Color Anti-Rabbit Automation IHC; OpalTM Polymer Anti-Rabbit HRP Secondary Antibody Kit; Akoya Biosciences®). The following primary rabbit antibodies were used for staining ...
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bioRxiv - Immunology 2022Quote: ... secondary HRP-labeled antibody and detection with optimized fluorescent Opal tyramide signal amplification (TSA) dye (Opal 7-color Automation IHC kit, from Akoya, Ref. NEL821001KT) and repeated antibody denaturation cycles ...
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bioRxiv - Neuroscience 2021Quote: ... the RNAscope probe for TNFR1 (product #408111) and the TSA Cyanine 3 Plus Evaluation kit (product #NEL744001KT, Akoya Biosciences, Boston, MA, USA). After completing the FISH protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were then washed for 3 x 5 minutes in NTT and developed with Cy5-Tyramide signal amplification reagent (TSA Plus Kit, Akoya Biosciences #NEL745001KT) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Opal 780) at a 1:250 dilution made in tyramide signal amplification reagent (Akoya, #FP1498) for 60 min ...
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bioRxiv - Neuroscience 2022Quote: ... slides were incubated with Opal fluorophore-conjugated tyramide signal amplification (TSA; 1:100, Akoya Biosciences). After the application of tyramides ...
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bioRxiv - Neuroscience 2022Quote: ... followed by tyramide-conjugated Cy3 (1:50) diluted in TSA amplification diluents (Akoya Biosciences, NEL741001KT). Sections were mounted ...
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bioRxiv - Developmental Biology 2023Quote: ... Signal amplification was done with 1:50 TSA Plus Cyanine-3 or -5 (Akoya Biosciences) for 1 h at RT ...
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bioRxiv - Neuroscience 2024Quote: ... The signals were amplified by a 1:70 TSA-plus Cyanine 3 (#NEL744001KT; AKOYA Bioscience) for 25 minutes ...
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bioRxiv - Physiology 2023Quote: Immunohistochemical staining for insulin and glucagon was conducted using a Tyramide Signal Amplification (TSA) Plus Biotin kit according to the manufacturer’s protocol (NEL700A001KT, Akoya Biosciences, Marlborough, MA, USA), as previously described [15] ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were then rinsed through two rounds of incubation in distilled water for 2 minutes and stored in Hydration Buffer from the PhenoCycler Staining Kit (Akoya Biosciences, MA, #7000008) until ready for staining.
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bioRxiv - Immunology 2021Quote: ... and PanCK was performed using the Motif PD-1/PD-L1 panel (Akoya Biosciences OP-000001) on a Leica Bond Rx instrument (Leica Biosystems ...
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bioRxiv - Neuroscience 2022Quote: ... and 1hr incubation with TSA plus Cyanine-3 (1:300; cat# NEL744001KT; Akoya Biosciences, Marlborough, MA) was performed ...
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bioRxiv - Microbiology 2022Quote: ... The following fluorophores were used during incubations: Opal 520 Reagent Pack (1:1500; Akoya Biosciences, FP1487001KT) for COX I detection ...
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bioRxiv - Neuroscience 2023Quote: ... slices were incubated in tyramide conjugated fluorescein (1:50) in TSA amplification diluent (Akoya Biosciences, NEL741001KT) for 10min at RT ...
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bioRxiv - Immunology 2023Quote: All antibodies used for multiplexed immunofluorescence were validated individually first by chromogenic singleplex immunohistochemistry (IHC) and then by singleplex immunofluorescence using the Opal 7 kit reagents (NEL871001KT, Akoya Biosciences, Waltham, MA, USA). Detailed methods and antibody information are in supplementary data ...
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bioRxiv - Genomics 2020Quote: ... Fluorescent labeling was conducted using Opal 480 (GFAP) and Opal 570 (CD44) dyes (1:150; Akoya Biosciences) for 10 minutes at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... Tyramide Signal Amplification (TSA) solutions were prepared as follows: 1/500 for TSA-Fluorescein (Akoya Biosciences, NEL741001KT), 1/2000 for TSA-CY3 (Akoya Biosciences ...
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bioRxiv - Neuroscience 2022Quote: ... All probes were detected using a 1:750 dilution of TSA plus fluorescein 488 (Akoya Biosciences NEL741001KT). Slides were then dried overnight ...
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bioRxiv - Neuroscience 2022Quote: ... washed again (2 x 5 min) and incubated with OPAL 650 Tyramide (Akoya Biosciences, FP1496001KT, 1:500) for 10 minutes ...
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bioRxiv - Immunology 2023Quote: ... Sections were stained with probes against CCL2 (channel 1; ACD 423811) conjugated to Opal 690 (Akoya FP1497001KT), CDKN1A (channel 2 ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-ER (Cell Marque, 249-R-15-ASR, clone: SP1, 1:70 dilution) with Opal 650 (Akoya Biosciences ...
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bioRxiv - Cell Biology 2024Quote: ... The ER was then visualized incubating the cells using fluorescent tyramide (TSA-FITC 1:200, AKOYA Biosciences), 0.003 H202 and 0,05% Tween-20 in 100 mM borate buffer ...