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Citations for Akoya Biosciences :
1 - 50 of 151 citations for Human G Protein Coupled Receptor Family C Group 6 Member A GPRC6A ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... CA) using probes against the leptin receptor mRNA (Cat# 415951) and Opal 650 dye kit (Akoya Biosciences, Marlborough, MA) to label LepR mRNA ...
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bioRxiv - Genomics 2024Quote: ... For multiplexed IHC staining the Opal 6-plex Detection Kit (Akoya Biosciences) was used ...
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bioRxiv - Immunology 2022Quote: ... was stained with 6 protein markers (Figure 4A) and captured with PhenoImager® imaging system (Akoya Biosciences). Over 15,000 cell type annotations from 35 training set slides were used as a training set ...
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bioRxiv - Cancer Biology 2023Quote: ... Multiplexed staining was performed using the Opal 6-plex detection kit (Akoya Biosciences NEL871001KT) on a Bond Rx Research Stainer (Leica Biosystems) ...
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bioRxiv - Cell Biology 2023Quote: ... and Opal 6-Plex Detection Kit for Whole Slide Imaging (Akoya Biosciences, Marlborough, MA, USA) were employed for the staining process ...
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bioRxiv - Cancer Biology 2023Quote: ... which was performed using an Opal 6-Plex Detection Kit (Akoya Biosciences, Menlo Park, CA) with the following Abs ...
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bioRxiv - Cancer Biology 2024Quote: The multi-IF was performed using the Opal 6-Plex Manual Detection Kit (NEL811001KT, Akoya), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Multispectral fluorescence staining was performed using the Opal 6-Plex Detection kit (Akoya Biosciences, Marlborough, MA) using formalin-fixed ...
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bioRxiv - Immunology 2024Quote: Multiplexed immunohistochemistry (mIHC) assay was performed using the Opal 6-Plex Detection Kit (AKOYA #811001, USA) as described previously73 ...
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bioRxiv - Cancer Biology 2024Quote: ... Staining was performed on a Leica BOND RX autostainer according to the modified Opal 7-color (v5.2 plus) preset protocol using the Opal 6-Plex Detection kit for Whole Slide Imaging (Akoya Biosciences). Prior to the first staining round ...
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bioRxiv - Immunology 2020Quote: We stained 5-µm-thick formalin-fixed paraffin-embedded sections from human xenograft tumors (UMSCC-1) using Opal multiplex kits (PerkinElmer/Akoya), for a panel of DAPI ...
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bioRxiv - Cancer Biology 2022Quote: ... 6-diamidino-2-phenylindole (Spectral DAPI, Akoya Biosciences) was applied per provided protocols to label nuclei ...
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bioRxiv - Systems Biology 2023Quote: ... 6-diamidino-2-phenylindole (Spectral DAPI, Akoya Biosciences) was applied per provided protocols to label nuclei ...
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bioRxiv - Cancer Biology 2024Quote: ... the 6-plex TSA with Opal dyes (Akoya) 6-plex and the 8-plex Signal Star (CST) ...
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bioRxiv - Cancer Biology 2023Quote: ... S and G blockers (Akoya Biosciences, MA, #7000008). Samples were first allowed to equilibrate to room temperature in Staining Buffer for 20-30 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... J and G blocking solutions to CODEX staining buffer (Akoya Bioscience). Antibodies were then added to this blocking buffer in a 1:50 titration to make a total volume of 100 µl ...
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bioRxiv - Cancer Biology 2021Quote: ... the slides were counterstained with 4’,6-diamidino-2-phenylindole (DAPI, Akoya Biosciences) and coverslipped using ProLong Gold antifade mounting media (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... the HRP-coupled secondary anti-rat OmniMap antibody (Ventana Medical Systems) for 12 min and finally Opal 520 fluorophore (Akoya Biosciences, 1:100) at RT for 8 min ...
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bioRxiv - Immunology 2023Quote: ... followed by probing with an appropriate OPAL fluorophore-conjugated secondary Ab (Akoya Biosciences, Supplementary Table 6). This was followed by a further HIER to remove the primary Ab before staining with the next primary/secondary Ab combination ...
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bioRxiv - Immunology 2022Quote: ... The standard Akoya CODEX protocols were followed (Akoya CODEX manual, revision C). For tyramide-barcode staining ...
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bioRxiv - Neuroscience 2021Quote: ... Opal 570 dye (Akoya Biosciences; 1:1500; 30 min at 40°C), followed by HRP blocker (15 min at 40°C) ...
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bioRxiv - Genetics 2022Quote: ... samples were incubated for 30 min at 40°C with Opal 620 (AKOYA Biosciences ...
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bioRxiv - Immunology 2021Quote: ... protein blocking in Akoya Antibody Diluent/Block (Akoya biosciences, MA) took place for 10 minutes ...
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bioRxiv - Developmental Biology 2021Quote: ... with probe for human TINAGL1(Advanced Cell Diagnostics #857221-C2) and OPAL 570 fluorophore (Akoya #FP1488001KT), following the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2024Quote: ... The reduction solution was removed by centrifugation (12,000 x g) and exchanged with the conjugation solution (Akoya Biosciences, 7000009). Each oligonucleotide barcode was rehydrated in 10 µl nuclease-free water (Life Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated at 40°C for 30 min with Opal 570 Dyes (Akoya Biosciences ...
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bioRxiv - Genomics 2020Quote: ... and human retina background fluorescence slide was used to spectrally unmix images in InForm software (Akoya Biosciences) for visualization of each color.
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bioRxiv - Cancer Biology 2023Quote: ... Protein blocking was performed using antibody diluent/blocking buffer (Akoya, ARD1001EA) for 10 minutes at room temperature ...
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bioRxiv - Physiology 2023Quote: ... sections were incubated in HRP-C2 at 40°C for 15mins followed by Opal650 (Akoya) in TSAP diluent for 30mins at 40°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Epitope retrieval/microwave treatment (MWT) for all antibodies was performed by boiling slides in Antigen Retrieval buffer 6 (AR6 pH6; Akoya, AR6001KT). Protein blocking was performed using antibody diluent/blocking buffer (Akoya ...
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bioRxiv - Neuroscience 2023Quote: ... The probe combination used was human specific SCN9A (Cat 562251, ACD) paired with Cy3 (Cat NEL744001KT, Akoya Biosciences) and TACR1 (Cat 310701-3 ...
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bioRxiv - Cancer Biology 2022Quote: ... at 85°C prior to the next staining cycles and finally stained with fluorescent DAPI (Akoya) for 10 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... at 85°C prior to the next staining cycles and finally stained with fluorescent DAPI (Akoya) for 10 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... and imaging were completed in accordance with Phenocycler manufacturer instructions (Akoya Biosciences User Manual, Revision-C)38 ...
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bioRxiv - Cell Biology 2023Quote: PhenoCycler-Fusion (PCF) on Human Tissues: All reagents in this section were purchased and used as received from Akoya Biosciences unless otherwise noted ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections underwent rounds of antigen stripping via incubation at 100 °C in AR9 buffer (Akoya Biosciences, AR900250ML) for 25 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... The tissue sections were then fixed and stored at 4℃° C in Storage Buffer (Akoya Biosciences, MA) until image acquisition ...
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bioRxiv - Genomics 2023Quote: Formalin fixed paraffin embedded human breast tissue was analyzed using COdetection by inDEXing (CODEX®/PhenoCycler - Akoya Biosciences, Marlborough, MA). The experiments were performed following manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2021Quote: Tissue sections were baked for 3 hours at 62°C in vertical slide orientation with subsequent deparaffinization performed on the Leica Bond RX followed by 30 minutes of antigen retrieval with Leica Bond ER2 followed by 6 sequential cycles of staining with each round including a 30-minute combined block and primary antibody incubation (Akoya antibody diluent/block ARD1001).
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bioRxiv - Cancer Biology 2023Quote: 4 µm FFPE tissue sections were baked for 2.5 hours at 63°C in vertical slide orientation with subsequent deparaffinization performed on the Leica Bond RX followed by 30 min of antigen retrieval with Leica Bond ER2 followed by 6 sequential cycles of staining with each round including a 30-minute combined block and primary antibody incubation (Akoya antibody diluent/block ARD1001EA) except HER2 ...
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bioRxiv - Developmental Biology 2023Quote: ... rinsed in wash buffer and incubated at 40°C for 30 minutes in OPAL flurophore 520 (Akoya Biosciences) diluted 1 in 100 with TSA diluent ...
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bioRxiv - Cancer Biology 2023Quote: Opal Multiplex IF Kit (Akoya) was used to perform multiplex immunofluorescence according to manufacturer’s instructions ...
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bioRxiv - Pathology 2019Quote: ... 7-color manual IHC kit (Akoya Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... TSA-Cy3 Plus kit (Akoya #NEL744001KT) was used to detect anti-DIG antibody at 1:200 ...
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bioRxiv - Immunology 2022Quote: ... Antigen retrieval was performed by boiling samples to 100°C for 15 minutes in pH 9 Antigen retrieval buffer (Akoya). Tissues were incubated for 45 minutes at room temperature with primary antibodies specific for CD20 (L26 ...
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bioRxiv - Molecular Biology 2022Quote: ... The hybridization detection was carried out using Opal Fluorophore Reagent Packs Opal-520 (channel (C) 1)/570 (C2)/690 (C3) (1:1500) (Akoya Biosciences). Probes (Advanced Cell Diagnostics ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were washed for 2 days at 4◦C and then incubated for 5 minutes with TSA Cyanine 5 at room temperature (NEL705A001, Akoya, biosciences). All embryos were nuclei stained with DAPI 1:1000 diluted in PBS ...
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bioRxiv - Systems Biology 2023Quote: ... Proximal protein biotinylation occurs with treatment of H2O2 and tyramide-biotin (TSA Biotin System, Akoya Bioscience NEL700A001KT), resulting in tyramide-biotin radicalization and covalent deposition onto proximal proteins ...
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bioRxiv - Pathology 2022Quote: ... using CODEX conjugation kit (Cat#: 7000009, Akoya) following CODEX conjugation manual.
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bioRxiv - Neuroscience 2021Quote: ... 570 and 690 reagent kits (Akoya Biosciences). Cells were stained with DAPI (ACD ...