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Citations for Akoya Biosciences :
1 - 50 of 190 citations for Fab ZAP mouse Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... antibodies were conjugated using Akoya Antibody Conjugation Kit (Akoya Biosciences, SKU 7000009) with a barcode (Akoya Biosciences ...
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bioRxiv - Neuroscience 2024Quote: ... after which the hybridized probes were detected using peroxidase conjugated anti-FLU or anti-DIG Fab(2) fragments and the Tyramide signal amplification (TSA) system from Akoya Biosciences ...
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bioRxiv - Immunology 2021Quote: ... A mixture of anti-Mouse + anti-Rabbit HRP-conjugated secondary antibodies (Akoya Biosciences) was added for 10 minutes ...
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bioRxiv - Immunology 2021Quote: The antibody panel was composed of commercially available AKOYA-conjugated antibodies and self-conjugated custom antibodies (CODEX Conjugation Kit, AKOYA Biosciences). Tissue staining was performed with the CODEX Staining Kit (AKOYA Biosciences) ...
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bioRxiv - Systems Biology 2023Quote: ... Oligo-conjugated primary antibodies were prepared 50µg at a time using the Akoya Antibody Conjugation Kit (Akoya Biosciences, Delaware, USA) according to the manufacturer’s recommended protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Additional antibodies were obtained from commercial vendors as indicated and conjugated to custom oligo barcodes according to instructions in Akoya’s antibody conjugation kit (Conjugation kit, Akoya, #7000009). Briefly ...
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bioRxiv - Immunology 2023Quote: ... Antibody binding was visualized using secondary anti-mouse/anti-rabbit polymer HRP followed with TSA-Opal reagents (Akoya NEL811001KT). Tissue slides were incubated with DAPI as a counterstain and coverslipped with VectaShield mounting media (Vector Labs) ...
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bioRxiv - Immunology 2021Quote: ... antibodies was performed using the OPAL Automation Multiplex IHC Detection Kit (Akoya Biosciences, Catalog 160 #NEL830001KT) implemented onto a BOND Research Detection System (DS9455) ...
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bioRxiv - Microbiology 2023Quote: ... Each antibody was optimized using Opal 3-Plex Anti-Rb Detection Kit (Akoya Biosciences Inc. cat# NEL830001KT) and stained with automated LabSatTM Research (Lunaphore Technologies SA ...
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bioRxiv - Cancer Biology 2023Quote: ... incubation times and appropriate antibody concentrations were optimized using the Opal™ 5-color kit (Akoya Biosciences, NEL840001KT). Epitope stability was assessed to determine the order of each antibody in sequence ...
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bioRxiv - Cancer Biology 2023Quote: Breast tumour sections were stained with antibodies using the Opal 7-7-colour multiplex immunohistochemistry kit (NEL811001KT, Akoya Biosciences). Staining was performed manually per the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... All antibodies (including pre-conjugated antibodies from Akoya) were additionally validated on the respective GBM control samples using multiple CODEX runs to adjust antibody concentration and exposure times to optimize signal to noise ratio ...
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bioRxiv - Pathology 2024Quote: ... we stained antibodies on tonsil tissue and aorta tissue microarray using the sample kit for PhenoCycler-Fusion (Akoya Biosciences, 7000017) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... All other antibodies were custom conjugated to their respective CODEX barcode (Supplemental Table S5) according to Akoya’s PhenoCycler-Fusion user guide using the antibody conjugation kit (Akoya, 7000009) following manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: Antigen retrieval and blocking were performed on the dewaxed slides using the Antigen retrieval reagent (pH 6.0) and Blocking/antibody diluent provided in the Opal Polaris Multicolor Manual IHC Detection Kit (Akoya Biosciences, USA), following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: All fixed frozen tissue CODEX antibody stainings were done according to Akoya Biosciences staining protocol associated with the CODEX Staining Kit with some modifications (Akoya; 7000008). After tissue hydration ...
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bioRxiv - Cancer Biology 2024Quote: mIHC staining was performed according to the manufacturer’s instructions (OpalTM 4-Color Anti-Rabbit Automation IHC; OpalTM Polymer Anti-Rabbit HRP Secondary Antibody Kit; Akoya Biosciences®). The following primary rabbit antibodies were used for staining ...
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bioRxiv - Immunology 2022Quote: ... secondary HRP-labeled antibody and detection with optimized fluorescent Opal tyramide signal amplification (TSA) dye (Opal 7-color Automation IHC kit, from Akoya, Ref. NEL821001KT) and repeated antibody denaturation cycles ...
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bioRxiv - Cancer Biology 2023Quote: ... NRG1 and TGFa primary antibodies were detected with HRP-conjugated antibody (ARR1001KT, Akoya Biosciences) and TSA-based amplification with Opal dye 690 (FP1497001KT ...
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bioRxiv - Immunology 2022Quote: ... conjugated primary antibodies cocktail (Akoya CD3e 1:100 and Akoya CD20 1:200 in Abcam Antibody Diluent ...
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bioRxiv - Molecular Biology 2023Quote: For antibodies purchased from Akoya Biosciences ...
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bioRxiv - Neuroscience 2021Quote: ... HRP-conjugated anti-flu antibody (AKOYA Bioscience NEF710001EA ...
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bioRxiv - Cell Biology 2021Quote: Antibodies were purchased preconjugated from Akoya Biosciences or sourced from other vendors and conjugated in-house using the CODEX Conjugation Kit (Akoya Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: Antibody conjugation requires reagents from Akoya Biosciences ...
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bioRxiv - Immunology 2022Quote: ... conjugated primary antibodies cocktail (Akoya CD3e 1:100 and Akoya CD20 1:200 in Abcam Antibody Diluent) 2x for 90 minutes each ...
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bioRxiv - Developmental Biology 2024Quote: The high-parametric antibody panel consisted of both a selection of pre-conjugated antibodies (n=23, Akoya Biosciences) and antibodies available through other commercial sources that were conjugated in house (n=49 ...
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bioRxiv - Cell Biology 2022Quote: ... a secondary HRP antibody (ARH1001EA, AKOYA Biosciences) followed by Opal 520 fluorophore (FP1487001KT ...
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bioRxiv - Neuroscience 2022Quote: ... diluted in Antibody Diluent/Block (Akoya, ARD1001EA) for 30min at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... five conjugated antibodies were purchased from Akoya Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... All antibodies not commercially available from Akoya and hence requiring custom-conjugation with DNA-barcodes were first validated with conventional immunofluorescence (Supplementary Note 2) ...
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bioRxiv - Cancer Biology 2023Quote: ... Opal Polymer HRP secondary antibody (AKOYA Biosciences), Opal Fluorophore (AKOYA Biosciences) ...
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bioRxiv - Cancer Biology 2022Quote: ... 30 minute incubation with the secondary antibody (Akoya), 10 minute incubation with the respective OPAL (Akoya ...
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bioRxiv - Cancer Biology 2022Quote: ... 30 minute incubation with the secondary antibody (Akoya), 10 minute incubation with the respective OPAL (Akoya ...
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bioRxiv - Cancer Biology 2023Quote: ... The slides were washed and anti-mouse/anti-rabbit HRP polymer (Akoya Biosciences) was added for 10 min at room temperature ...
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bioRxiv - Genomics 2021Quote: ... Slides were incubated in primary antibody overnight and secondary antibody conjugated to peroxidase and then developed using Tyramide Signal Amplification (TSA, Akoya Biosciences). Slides were counter stained with DAPI ...
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bioRxiv - Cancer Biology 2022Quote: ... Endogenous peroxidase activity was blocked with 3% hydrogen peroxide prior to the addition of any primary antibodies which were prepared in the Opal Blocking/Antibody Diluent (Akoya biosciences). A pan-immune antibody panel and a macrophage subset antibody panel were used to investigate immune cell localization in brain metastasis samples ...
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bioRxiv - Developmental Biology 2024Quote: ... Slides were incubated in primary antibody overnight and secondary antibody conjugated to peroxidase and then developed using Tyramide Signal Amplification (TSA, Akoya Biosciences). Samples were stained with either hematoxylin and eosin or the following antibodies ...
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bioRxiv - Genomics 2024Quote: ... Slides were incubated for 1 hour at room temperature with primary antibodies diluted in 1x Opal blocking/antibody diluent (Akoya biosciences). Slides were subsequently incubated with the Opal Polymer HRP Ms + Rb secondary polymer for 30 minutes prior to incubation with Opal fluorophores (Opal 520 ...
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bioRxiv - Cancer Biology 2023Quote: Opal Multiplex IF Kit (Akoya) was used to perform multiplex immunofluorescence according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... followed by incubation with the first primary antibody for 1 hour, subsequently with the secondary antibody (Polymer HRP, Ms + Rb (Akoya biosciences, MA)) for 30 minutes and finally with an Opal fluorophore ((Akoya biosciences ...
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bioRxiv - Immunology 2022Quote: Commercially available oligonucleotide conjugated antibodies were purchased from Akoya Biosciences and used at a standard dilution of 1/200 (Supplemental Table 1) ...
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bioRxiv - Cancer Biology 2023Quote: Commercially available oligo-conjugated antibodies were obtained from Akoya Biosciences (Marlborough ...
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bioRxiv - Developmental Biology 2024Quote: ... 100 μl of antibody storage buffer (Akoya Biosciences, 7000009) was added on top of antibodies and centrifuged (3000 x g ...
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bioRxiv - Pathology 2019Quote: ... 7-color manual IHC kit (Akoya Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... TSA-Cy3 Plus kit (Akoya #NEL744001KT) was used to detect anti-DIG antibody at 1:200 ...
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bioRxiv - Cancer Biology 2021Quote: Tissue sections were baked for 3 hours at 62°C in vertical slide orientation with subsequent deparaffinization performed on the Leica Bond RX followed by 30 minutes of antigen retrieval with Leica Bond ER2 followed by 6 sequential cycles of staining with each round including a 30-minute combined block and primary antibody incubation (Akoya antibody diluent/block ARD1001).
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bioRxiv - Cancer Biology 2023Quote: 4 µm FFPE tissue sections were baked for 2.5 hours at 63°C in vertical slide orientation with subsequent deparaffinization performed on the Leica Bond RX followed by 30 min of antigen retrieval with Leica Bond ER2 followed by 6 sequential cycles of staining with each round including a 30-minute combined block and primary antibody incubation (Akoya antibody diluent/block ARD1001EA) except HER2 ...
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bioRxiv - Immunology 2024Quote: ... Biotinylated antibodies were visualized with Streptavadin-AF594 (Cat. No.: S32356) and the CD4 antibody was visualized with OPAL 520 (Akoya Biosciences, Cat. No.: FP1487001KT). The ROI’s selected to undergo GeoMx® DSP spatial transcriptomics were chosen based on Lyve-1 staining ...
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bioRxiv - Physiology 2022Quote: ... antibodies were reduced using a “Reduction Master Mix” (Akoya Biosciences) to which lyophilized barcodes resuspended in molecular biology grade water and “Conjugation Solution” (Akoya Biosciences ...
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bioRxiv - Physiology 2022Quote: ... Antibodies were imaged cyclically using the CODEX system from Akoya Biosciences and a Keyence BZ-X810 slide scanning microscope fitted with a 20x air objective (0.75 NA) ...